• Journal of IKEEE
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• v.23 no.4
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• pp.1430-1439
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• 2019

In Functional Mockup Interface(FMI)-based co-simulation, a bisectional algorithm can be used to find the zerocrossing point as a way to improve the accuracy of the simulation results. In this paper, the proposed master algorithm(MA) analyzes the repeated interval graph and predicts the next interval by applying the Markov Chain to the step size. In the simulation, we propose an algorithm to minimize the rollback by storing the step size that changes according to the graph type as an array and applying it to the next prediction interval when the rollback occurs in the simulation. Simulation results show that the proposed algorithm reduces the simulation time by more than 20% compared to the existing algorithm.

• Journal of the Korean Society for Precision Engineering
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• v.19 no.2
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• pp.26-32
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• 2002

• Journal of Microbiology and Biotechnology
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• v.26 no.12
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• pp.2184-2191
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• 2016

The time-consuming and high-cost preparation of soluble peptide-major histocompatibility complexes (pMHC) currently limits their wide uses in monitoring antigen-specific T cells. The single-chain trimer (SCT) of peptide-${\beta}2m$-MHC class I heavy chain was developed as an alternative strategy, but its gene fusion is hindered in many cases owing to the incompatibility between the multiple restriction enzymes and the restriction endonuclease sites of plasmid vectors. In this study, overlap extension PCR and one-step cloning were adopted to overcome this restriction. The SCT gene of the $OVA_{257-264}$ peptide-$(GS_4)_3-{\beta}2m-(GS_4)_4-H-2K^b$ heavy chain was constructed and inserted into plasmid pET28a by overlap extension PCR and one-step cloning, without the requirement of restriction enzymes. The SCT protein was expressed in Escherichia coli, and then purified and refolded. The resulting $H-2K^b/OVA_{257-264}$ complex showed the correct structural conformation and capability to bind with $OVA_{257-264}$-specific T-cell receptor. The overlap extension PCR and one-step cloning ensure the construction of single-chain MHC class I molecules associated with random epitopes, and will facilitate the preparation of soluble pMHC multimers.

• Journal of Korean Society for Quality Management
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• v.41 no.1
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• pp.39-52
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• 2013

Purpose: The purpose of this study was to investigate if purchasing companies' efforts of supplier development activities to supplier companies have positive impacts on the purchasing companies' performance as the first step. In the second step, we tried to confirm if the concept of Supply Chain Corporate Social Responsibility activities and Coexistence activities take the roles of moderate variable on relationship between supplier development and three performances respectively. Methods: The collected data through survey were analysed using multiple regression for the first step of the study and moderate regression for the second one of it. Results: The results of this study are as follows; supplier development efforts effect on all three performances positively. Moreover, Supply Chain CSR has significant moderate effect on relationship between supplier management and corporate performances, while Coexistence does between supplier management and logistics performances. In the case of relationship between supplier management and production performances, both Supply Chain CSR and Coexistence show significant moderate effect. Conclusion: Manufacturing companies in Korea need to make effort of supplier development in selective way when they want to practice Supply Chain CSR and Coexistence concurrently considering strategies and objectives.

• Macromolecular Research
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• v.10 no.6
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• pp.297-303
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• 2002

We have investigated the microstructural properties of a weakly charged polyelectrolyte modeled with both Hookean spring and Debye-Huckel potential, by employing a novel hybrid scheme of molecular dynamics (MD) and Monte Carlo (MC) simulations. Although the off-lattice pivot step facilitates the earlier computations stage, it gives rise to oscillations and hinders the stable equilibrium state. In order to overcome this problem, we adopt the MC off-lattice pivot step in early stage only, and then switch the computation to a pure MD step. The result shows that the computational speed-up compared to the previous method is entirely above 10 to 50, without loss of the accuracy. We examined the conformations of polyelectrolyte in solvents in terms of the end-to-end distance, radius of gyration, and structure factor with variations of the screening effects of solvent and the monomer charges. The emphasis can favorably be given on the elongation behavior of a polyelectrolyte chain, with observing the simultaneous snapshots.

• Journal of Information Processing Systems
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• v.18 no.2
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• pp.173-187
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• 2022

A special government agency has been charged with implementing quality management to guarantee the quality of wild-simulated ginseng. However, these processes are carried out by use of documents, and this has resulted in information omission and high document management costs. To solve this problem, this study analyzed the existing quality management process by using a smart contract for the existing offline form and proposed a new quality management system for storing and managing all log data in the blockchain. This system reduced documentation management costs about quality management and recorded information in the previous step through the quality management steps, thus forming a step-by-step record chain. Experiments were conducted by implementing this system, which improved data integrity and reliability. Additionally, sensitive information, such as personal information, was included in the system by use of the off-chain technology.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.9
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• pp.4180-4196
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• 2017

In this paper, we analyze average capacity of an amplify-and-forward (AF) cooperative communication system model in multi-relay multiuser networks. In contrast to conventional cooperative networks, relays in the considered network have no embedded energy supply. They need to rely on the energy harvested from the signals broadcasted by the source for their cooperative information transmission. Based on this structure, a two-step selection scheme is proposed considering both channel state information (CSI) and battery status of relays. Assuming each relay has infinite or finite energy storage for accumulating the energy, we use the infinite or finite Markov chain to capture the evolution of relay batteries and certain simplified assumptions to reduce computational complexity of the Markov chain analysis. The approximate closed-form expressions for the average capacity of the proposed scheme are derived. All theoretical results are validated by numerical simulations. The impacts of the system parameters, such as relay or user number, energy harvesting threshold and battery size, on the capacity performance are extensively investigated. Results show that although the performance of our scheme is inferior to the optimal joint selection scheme, it is still a practical scheme because its complexity is much lower than that of the optimal scheme.

• Korean Journal of Veterinary Service
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• v.34 no.1
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• pp.13-18
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• 2011

The purpose of this study was to apply a nested-polymerase chain reaction (nPCR) assay to detect and differentiate PCV 2a and PCV 2b. The compared with nPCR and one-step PCR and nPCR showed more sensitive in the detection of PCV-2 from tissue and blood samples. The total of 52 tissue samples was collected from postweanning pigs from 2006 to 2010. All tissue samples showed positive for PCV-2 in one-step PCR and nPCR, followed by the nPCR in order to identify the genotypes of PCV-2. 2 samples (3.8%) showed positive for PCV 2a, and 35 samples were positive for PCV 2b (67.3%), 15 samples (28.9%) were positive the dual genotypes. In addition, 42 blood samples which were collected from the 5 different swine farms were compared figure out the detection rates of nPCR and one-step PCR. The PCV 2 was positive by one-step PCR in 21 samples (50.0%) and nPCR was positive in 37 samples (88.1%). The PCV 2 genotypes in blood samples and 32 samples (76.2%) were positive for PCV 2b and none were positive for PCV 2a, 5 samples (11.9%) were positive for dual genotypes. These results suggest that the nPCR is very efficient for genotyping blood samples and differentiating the genotypes of PCV-2 from field samples.

• Communications for Statistical Applications and Methods
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• v.17 no.3
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• pp.441-450
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• 2010

In this paper we consider the problem of forecasting binomial time series, modelled by the binomial autoregressive model. This paper considers proposed by McKenzie (1985) and is extended to a higher order by $Wei{\ss}$(2009). Since the binomial autoregressive model is a Markov chain, we can apply the earlier work of Bu and McCabe (2008) for integer valued autoregressive(INAR) model to the binomial autoregressive model. We will discuss how to compute the h-step-ahead forecast of the conditional probabilities of $X_{T+h}$ when T periods are used in fitting. Then we obtain the maximum likelihood estimator of binomial autoregressive model and use it to derive the maximum likelihood estimator of the h-step-ahead forecast of the conditional probabilities of $X_{T+h}$. The methodology is illustrated by applying it to a data set previously analyzed by $Wei{\ss}$(2009).

• Safety and Health at Work
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• v.12 no.4
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• pp.544-545
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• 2021

The article by Tae-Gu Kim et al. conducted elastic FE modeling, which was inappropriate for fracture of elastic-plastic chain material (11.3% of elongation). FE analysis results and the findings in the fracto-graphic analysis did not tally but contradicted each other. The article identified "incorrect installation"/bending forces as the root cause while FE results of the chain under bending forces showed very low stresses at fracture locations but the highest stress in the middle of shank of the chain. The article's "step-like topographies indicating the fracture due to bending moment rather than uniaxial tension" lacked scientific support. The load value carried by each chain section under bending/incorrect installation was only half of that under tension, thus the article using same load value in FE simulation comparison for bending and tension was incorrect. The real cause of the chain fracture was likely improper checking the lifted load or/and using the wrong chain with much lower safety working load.