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http://dx.doi.org/10.7853/kjvs.2011.34.1.013

Application of a nested-polymerase chain reaction assay to differentiate the genotypes of porcine circovirus 2  

Chu, Keum-Suk (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research)
Publication Information
Korean Journal of Veterinary Service / v.34, no.1, 2011 , pp. 13-18 More about this Journal
Abstract
The purpose of this study was to apply a nested-polymerase chain reaction (nPCR) assay to detect and differentiate PCV 2a and PCV 2b. The compared with nPCR and one-step PCR and nPCR showed more sensitive in the detection of PCV-2 from tissue and blood samples. The total of 52 tissue samples was collected from postweanning pigs from 2006 to 2010. All tissue samples showed positive for PCV-2 in one-step PCR and nPCR, followed by the nPCR in order to identify the genotypes of PCV-2. 2 samples (3.8%) showed positive for PCV 2a, and 35 samples were positive for PCV 2b (67.3%), 15 samples (28.9%) were positive the dual genotypes. In addition, 42 blood samples which were collected from the 5 different swine farms were compared figure out the detection rates of nPCR and one-step PCR. The PCV 2 was positive by one-step PCR in 21 samples (50.0%) and nPCR was positive in 37 samples (88.1%). The PCV 2 genotypes in blood samples and 32 samples (76.2%) were positive for PCV 2b and none were positive for PCV 2a, 5 samples (11.9%) were positive for dual genotypes. These results suggest that the nPCR is very efficient for genotyping blood samples and differentiating the genotypes of PCV-2 from field samples.
Keywords
Porcine circovirus 2 (PCV-2); One-step polymerase chain reaction (PCR); Nestedpolymerase chain reaction (nPCR);
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Times Cited By KSCI : 7  (Citation Analysis)
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