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Studies on Biological Activities of the Polysaccharides and Oligosaccharides of Orostachys japonicus (와송 다당체 및 올리고당류의 생리 활성)

  • Kim Ki Hoon;Kim Eun Young;Kim Yea Oon;Baek Geum Ok;Kim Han Bok;Lee Dong Seok
    • Korean Journal of Microbiology
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    • v.40 no.4
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    • pp.334-341
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    • 2004
  • Polysaccharides were prepared from Orostachys japonicus by extration with hot steam water (OJPl). The OIPl fraction was further purified by Sephadex G-50 gel filtration chromatography to produce FI (polysaccharides) and FII (oligosaccharides) fraction. The average molecular masses o fFI and FII fraction were determined to be 3050 kDa and 13 kDa, respectively. The antimicrobial activity of OIPl was tested against 8 strains of bacteria and one strain of yeast by the disc diffusion method, fluorescein diacetate (FDA) method and broth dilution method. The OIPl exhibited a very strong growth inhibition to Candida albicans. The OIPl remarkably sup­pressed the growth of Salmonella typhimurium and Staphylococcus aureus. The OIPl showed higher growth inhibition to Escherichia coli and Pseudomonas aeruginosa than propolis, positive control. When the anticancer activity of the OIPl, FI or FII was examined against human cancer cell lines and the Sarcoma 180 cells, these widely suppressed the proliferation of cell lines in the MTT assay and morphology study. Especially, they remarkably inhibited the growth of A549, HeLa and AGS cells. Also treatment of cancer cells with OJPl, FI or FII induced apoptotic cell death characterized by DNA fragmentation. The OJPl, FI or FII exhibiting various biological activities such as antimicrobial activity and anticancer activity is expected to be developed as new biohealth products.

Antimicrobial Activity and Distilled Components of Garlic(Allium sativum L.) and Ginger(Zingiber officinale Roscoe) (마늘과 생강의 항균성 및 증류성분)

  • Ji, Won-Dae;Jeong, Min-Seon;Chung, Hyun-Chae;Lee, Suk-Jeong;Chung, Yung-Gun
    • Applied Biological Chemistry
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    • v.40 no.6
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    • pp.514-518
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    • 1997
  • In order to investigate the possible use of spices as natural preservatives) antimicrobial activities of garlic and ginger, representative spices, were examinated. Distilled components of garlic and ginger were also analyzed. Each extract of garlic was more suppressive on the growth of Gram negative bacteria than that of Gram positive bacteria. Extracts of garlic were showed effective suppressions on the growth of yeasts. Of the extracts of garlic, ether extract was highest among antimicrobial activities. In the case of ginger, ether extract of ginger was effective for growth inhibition of tested bacteria. Distilled components of garlic and ginger were extracted by simultaneous steam distillation extraction apparatus. The concentrates were analyzed with GC/MSD and Kovat's retention index and identified 13 components and 21 components respectively. Distilled components of garlic and ginger were full of sulfur compounds and hydrocarbons, respectively. di-2-Propenyl disulfide, methyl 2-propenyl disulfide, 2-vinyl-4H-1,3-dithiirane and 3,3'-thiobis-1-propene were major principal aroma compounds in garlic. ${\alpha}-zingibirene$, ${\beta}-phellandrene$, ${\beta}-sesquiphellandrene$ and camphene were major principal compounds in ginger.

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Acute Toxicity of Emulsifiable Concentrate of Coriander Essential Oils against Cyprinus carpio (고수 정유를 원제로 하는 유제 제형의 잉어에 대한 급성독성)

  • Nam, Tae-Hoon;Jeon, Hwang-Ju;Kim, Kyeongnam;Choi, Yeonseo;Lee, Sung-Eun
    • Korean Journal of Environmental Biology
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    • v.34 no.3
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    • pp.208-211
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    • 2016
  • Essential oils (EOs) extracted from plants possess various biological activities and have been considered as natural insecticides due to their potent insecticidal activities. In regard to develop natural insecticides, EOs are formulated as an emulsifiable concentrate and their acute toxicity against to fishes were determined in a static condition using Cyprinus carpio. Coriander EO was used as an active ingredient mixed with ethanol for solvent and various surface active agents. The tested EOs were obtained from a commercial market, and three different extractions were also undertaken to produce EO using steam distillation, solvent extraction, and supercritical fluid extraction. Among the emulsifiable concentrate including a commercial coriander EO, surface active agents such as Tween 80, sodium dodecyl benzene sulfonate (SDBS), and mixture of SDBS and Nonidet showed acute toxicity to the fish. With the three different EO extraction, coriander EO obtained from supercritical fluids with Triton X-100 exhibited acute toxicity to C. carpio. Taken together, Tetgitol and Nondet are considered as surface active agents for the emulsifiable formulation of coriander EO.

Effect of Cu Addition in Cu/Fe/Zr-Mixed Metal Oxide Mediums for Two-step Thermochemical Methane Reforming (2단계 열화학 메탄 개질을 위한 Cu/Fe/Zr-혼합 산화물 매체 내 Cu 첨가 효과)

  • Cha, Kwang-Seo;Kim, Hong-Soon;Lee, Dong-Hee;Jo, Won-Jun;Lee, Young-Seak;Kim, Young-Ho
    • Applied Chemistry for Engineering
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    • v.18 no.6
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    • pp.618-624
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    • 2007
  • thermochemical methane reforming consisting of two steps on Cu/Fe/Zr mixed oxide media was carried out using a fixed bed infrared reactor. In the first step, the metal oxide was reduced with methane to produce CO, $H_2$ and the reduced metal oxide in the temperature of 1173 K. In the second step, the reduced metal oxide was re-oxidized with steam to produce $H_2$ and the metal oxide in the temperature of 973 K. The reaction characteristics on the added amounts of Cu in Cu/Fe/Zr mixed oxide media and the cyclic tests were evaluated. With the increase of the added amount of Cu in Cu/Fe/Zr mixed oxide media, the conversion of $CH_4$, the selectivity of $CO_2$ and the $H_2/CO$ molar ratio were increased, while the selectivity of CO was decreased in the first step. On the other hand, the evolved amount of $H_2$ was decreased with increasing the added amount of Cu in the second step. The $Cu_xFe_{3-x}O_4/ZrO_2$ medium added with Cu of x = 0.7 showed good regeneration properties in the 10th cyclic tests indicating that the medium had high durability. In addition, the gasification of the deposited carbon in the water splitting step was promoted with the addition of Cu in the media.

Growth of Plug Seedlings of Petunia 'Madness Rose' and Pansy 'Magestic GT' in Various Mixtures of Recycled Horticultural Media (원예용 폐배지를 재활용한 혼합배지에서 페튜니아와 팬지 플러그묘의 생육)

  • Shin, Woo Gun;Jeong, Byoung Ryong
    • Horticultural Science & Technology
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    • v.18 no.4
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    • pp.523-528
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    • 2000
  • Plug seedlings of Petunia hybrida 'Madness Rose' and Viola tricolor 'Magestic GT' were cultured in media containing various volume ratios of recycled plug medium, recycled coir, perlite, granular rockwool, and vermiculite for 36 and 43 days after sowing, respectively. Recycled plug medium and recycled coir were steam pasteurized for 30 minutes at $120^{\circ}C$ and 1.5 atmosphere. An unused commercial plug medium (Tosilee, pH 5.10, EC $0.12mS{\cdot}cm^{-1}$ at 1:5 dilution, v/v, Shinan Grow Co.) was used as the control. The pH of different media before and after growing seedlings was similar. Medium EC was high when recycled plug medium was included. Recycled coir (75%)+vermiculite (25%) mixture also had high medium EC. However, medium EC was low when granular rockwool or perlite was included. Height, root formation, shoot dry weight and leaf count (ea) of petunia, and height, total fresh and dry weights, and shoot fresh and root dry weights of pansy were the highest in recycled coir (75%)+perlite (25%) mixture. Recycled coir was better than recycled plug medium in physicochemical properties, and also in resultant plant growth. It is recommended to include perlite or granular rockwool when plug media including recycled horticultural media are prepared.

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The Long-term Durability Evaluation of PC Box for Near-surface Transit System manufactured by Microwave Heat curing (마이크로웨이브 발열양생에 의해 제작된 저심도 철도시스템용 PC BOX의 장기내구성 평가)

  • Koh, Tae-Hoon;Yoo, Han-Ju
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.4
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    • pp.766-771
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    • 2016
  • This study examined the long-term durability of PC boxes, which was manufactured by low-carbon eco-friendly concrete using an alternative binder to cement and alternative fine aggregate to sand and microwave heat curing system to reduce the construction cost of a near-surface transit system. Based on the test results, the initial compressive strength of microwave heat cured concrete was higher than that of the steam cured concrete, but those were similar in the long-term age. In addition, there was no significant difference between the two curing conditions in the chemical resistance and the freeze-thawing resistance, and the chloride ion penetration level of the concrete cured by two methods was very low. Therefore, low-carbon eco-friendly concrete and microwave heat curing technology are expected to contribute to the economic construction of a near-surface transit system, and reduce carbon dioxide emissions and environmental impact.

Simulation of the flue gas treatment processes of an industrial-waste incinerator using Aspen plus (Aspen plus를 이용한 산업폐기물 소각로의 배가스 처리 공정 모사)

  • Lee, Ju-Ho;Jung, Moon-Hun;Kwon, Young-Hyun;Lee, Gang-Woo;Shon, Byung-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.11
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    • pp.3246-3252
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    • 2009
  • The interest on the recovery of thermal energy using the waste has been rising to solve the problems of continuous increase of waste generation and the depletion of the fossil fuel recently. The incineration has been used most popularly as a treatment process of the waste for the energy recovery. However, it is expected that incineration and design cost will increase in the treatment of air contaminant emitted from incinerator. This research has simulated the actual incinerator and the flue gas treatment system using the Aspen plus which is the software to simulate the chemical process. The incineration process is composed of the 1st and 2nd combustor to burn the waste, SNCR process to reduce the $NO_x$ using the urea, and the steam generation process to save the energy during incineration. The $Ca(OH)_2$ slurry was used as an acid gas (HCl, $SO_2$) treatment materials and the removal efficiency for the products from the neutralization of acid gas in SDA and combustion ash was simulated at the bag filter. The simulation result has been corresponded with the treatment efficiency of emitted gas from the actual industrial waste incinerator and it is presumed to be used to forecast the efficiencies of flue gas treatment system in the future.

Comparison of Gingerols in Various Fractions and the Antioxidant Effects of Supercritical Fluid Extracts from Ginger (생강 분획의 gingerols 분석 및 초임계 추출물의 항산화 효과)

  • Lee, Eun-Ju;Yang, Seun-Ah;Choi, Hee-Don;Im, Hyo-Gwon;Whang, Key;Lee, In-Seon
    • Korean Journal of Food Science and Technology
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    • v.43 no.4
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    • pp.469-474
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    • 2011
  • Ginger (Zingiber officinale Rosc.) is a well known edible plant that is traditionally used to treat various illnesses related to inflammation and oxidative stress. Steam distillated ginger oil or water extract are mainly used for related products; however, it is unclear whether these fractions contain most of the bioactive compounds or the highest efficacy. This investigated the antioxidant effects of extracts prepared by supercritical fluid extraction (SFE). 6-Gingerol was the most abundant component in hexane fraction of ethanol extract from ginger. The antioxidative properties of SFE oil and Marc ethanol fractions were demonstrated using the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azinobis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals scavenging assays. A clear correlation was observed between total polyphenol contents and $RC_{50}$ values in the Marc ethanol fraction. These results indicate that not only SFE oil but the marc after SFE could be good sources for the food industry.

Zooplankton Grazing on Bacteria and Factors Affecting Bacterial C-flux in Lake Paldang Ecosystem (팔당호 생태계에서 동물플랑크톤의 박테리아 섭식 및 영향인자)

  • Uhm, Seong-Hwa;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.39 no.4 s.118
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    • pp.424-434
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    • 2006
  • This study investigates bacteria-zooplankton grazing link and factors affecting their grazing relationship at trophically different two sites (Paldang Dam and Kyungan Stream) of Lake Paldang Ecosystem from April to December, 2005. Zooplankton were divided into two size groups; microzooplankton (MICZ) : 60-200 ${\mu}m$ and macrozooplankton (MACZ): >200 ${\mu}m$), and their grazing rates on bacteria were conducted for each size group separately. Bacterial abundance and seasonal change pattern were similar between two sites. MICZ, mostly rotifers (e.g., Brachionus, Keratella, Polyathra) were numerically dominant at both sites, while carbon biomass was highest in cladocerans. Zooplankton biomass was higher at the Kyungan Steam site compared to Paldang Dam site, and their high biomass during spring decreased as they were passing through the storm events in summer season at both sites. Zooplankton clearance rate (CR) was high in spring and autumn while low in summer at Paldang Dam site. However, zooplankton CR was high during the summer at Kyungan Stream site. Bacterial C-flux was high in spring and autumn when MACZ (esp. cladecerans) developed at a high biomass level at both sites. Overall, MACZ community CR and carbon flux (C-flux) were higher than those of MICZ, and the degree of difference between them was higher at Kyungan Stream site. Short hydraulic residence time and physical disturbance caused by summer storm event appeared to affect the zooplankton grazing on bacteria at both sites. The results of this study indicate that bacteria are potentially important carbon source of zooplankton, and that both biotic (e.g,, prey and predator taxa composition and abundance) and physical parameters appear to alter energy transfer in the planktonic food web of this river-reservoir hybrid system.

Changes of Volatile Flavor Components on Roasting Conditions in Cassia tora Seeds (결명자 종실의 볶음조건에 따른 향기성분 변화)

  • Kim, Jong-Kuk;Hawer, Woo-Derck;Ha, Jae-Ho;Moon, Kwang-Deok;Chung, Shin-Kyo
    • Korean Journal of Food Science and Technology
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    • v.27 no.5
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    • pp.736-741
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    • 1995
  • This study was conducted to investigated the change of volatile flavor components of Cassia tora seeds during roasting treatments. The flavor components of Cassia tora seeds were analyzed by gas chromatography(GC) and combined gas chromatography-mass spectrometry(GC/MS). Among the flavor compounds collected by simultaneous steam distillation and extraction(SDE) method, 38 components were separated and identified. They consisted of 3 pyrazines, 4 pyrroles or pyridine, 4 alcohols, 11 aldehydes or ketones, 9 furans or phenols and 7 others. The flavor compounds collected from unroasted Cassia tora seeds were 7 components. During roasting process, many other flavor components were formed and increased in their contents. The contents of pyrazines and furans were highest and increased conspicuously, whereas, the contents of aldehydes, ketones, alcohols and pyridines were not increased significantly.

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