Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Acute Toxicity of Emulsifiable Concentrate of Coriander Essential Oils against Cyprinus carpio

고수 정유를 원제로 하는 유제 제형의 잉어에 대한 급성독성

  • Nam, Tae-Hoon (School of Applied Biosciences, Kyungpook National University) ;
  • Jeon, Hwang-Ju (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Kyeongnam (School of Applied Biosciences, Kyungpook National University) ;
  • Choi, Yeonseo (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Sung-Eun (School of Applied Biosciences, Kyungpook National University)
  • 남태훈 (경북대학교 응용생명과학부) ;
  • 전황주 (경북대학교 응용생명과학부) ;
  • 김경남 (경북대학교 응용생명과학부) ;
  • 최연서 (경북대학교 응용생명과학부) ;
  • 이성은 (경북대학교 응용생명과학부)
  • Received : 2016.09.04
  • Accepted : 2016.09.09
  • Published : 2016.09.30
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Abstract

Essential oils (EOs) extracted from plants possess various biological activities and have been considered as natural insecticides due to their potent insecticidal activities. In regard to develop natural insecticides, EOs are formulated as an emulsifiable concentrate and their acute toxicity against to fishes were determined in a static condition using Cyprinus carpio. Coriander EO was used as an active ingredient mixed with ethanol for solvent and various surface active agents. The tested EOs were obtained from a commercial market, and three different extractions were also undertaken to produce EO using steam distillation, solvent extraction, and supercritical fluid extraction. Among the emulsifiable concentrate including a commercial coriander EO, surface active agents such as Tween 80, sodium dodecyl benzene sulfonate (SDBS), and mixture of SDBS and Nonidet showed acute toxicity to the fish. With the three different EO extraction, coriander EO obtained from supercritical fluids with Triton X-100 exhibited acute toxicity to C. carpio. Taken together, Tetgitol and Nondet are considered as surface active agents for the emulsifiable formulation of coriander EO.

식물로부터 추출한 정유 (Essential oil, E. O.)는 다양한 생리활성을 나타내며 특히 해충에 대한 강한 살충 효과를 지니고 있어서 식물 정유를 이용한 해충방제 연구가 활발히 진행되고 있다. 해충방제 목적으로 정유를 사용하기 위하여 정유를 유제 (Emulsifiable Concentrate)형태로 개발하였고 이들의 수계 환경 중 급성어독성을 지수식으로 시험하였다. 유제 제형은 고수 정유 (Coriander E. O.)를 원제로 하였으며 용제로서 에탄올을 이용하였고 유화제로서 계면활성제를 사용하였다. 사용된 정유는 상업화된 정유, 연구실에서 수증기 증류법, 용매추출법, 초임계추출법을 이용하여 얻은 정유를 각각 실험에 사용하였다. 어독성 시험을 위하여 잉어를 이용하였으며 유제 제형 중 Tween 80, sodium dodecyl benzene sulfonate (SDBS) 및 SDBS와 Nonidet의 혼합형태를 계면활성제로 사용하였을 때, 상용화된 고수 정유가 잉어에 대한 급성독성을 나타내었다. 다양한 추출법에 의해서 얻은 고수 정유의 경우, 초임계추출법에 의하여 얻은 정유가 Triton X-100을 계면활성제로 사용하였을 때, 어독성을 나타내었다. 위의 결과를 토대로 본 시험에서 유화제로서 사용된 계면활성제 중 Tergitol과 Nonidet만이 고수 정유를 원제로 하는 유제 제형에 적합함을 확인할 수 있었다.

Keywords

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