• Microbiology and Biotechnology Letters
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• v.28 no.3
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• pp.139-146
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• 2000

산업적으로 유용한 미생물 유래 셀룰로오스를 생산 이용하기 위해 전국 각지 양조식초의 덧으로부터 세룰로오스의 생산성이 높고 조질의 균일성을 나타내는 균주를 분리하였다 분리균 GS11은 gram 음성이고 간균(0.6$\times$2.2~3.2 $\mu\textrm{m}$)의형태를 하고 있으며 편모를 가지고 있어 운동성을 보였다. 또한 세포내 지방산 조성은 다량의 불포화 지방산 {{{{ {C}_{18:1} }}}}과 포화지반산 {{{{ {C}_{16:0 } }}}}, {{{{ {C}_{14:0 } }}}} 이 대부분을 차지하였고 DNA 염기조성 (G+C) 함량은 58.4% 이였으며 ubiqunone 은 {{{{ { Q}_{10 } }}}}을 갖는 것으로 나타났다 이러한 형태학적 생리.생화학적 특성의 결과에 따라 본 균주는 Acetobacter xylinum GS11으로 동정되었다 A. xylinum, GS11 의배양기간 동안 셀룰로오스 생산성을 검토하고자 250mL 삼각플라스크에 균주를 접종하여 $30^{\circ}C$에서 12일간 정치배양하였다 그결과 기질인 glucose의 소비는 접종 후 급소하게 감소하여 셀룰로오스 생산에 이용되었으며 셀룰오스의 생산은 배양 9일 경에 2.8g/l로 최대의 생산량을 나타냈다.

• Steel and Composite Structures
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• v.15 no.1
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• pp.57-79
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• 2013

This paper studies the dynamic instability of laminated composite plates under thermal and arbitrary in-plane periodic loads using first-order shear deformation plate theory. The governing partial differential equations of motion are established by a perturbation technique. Then, the Galerkin method is applied to reduce the partial differential equations to ordinary differential equations. Based on Bolotin's method, the system equations of Mathieu-type are formulated and used to determine dynamic instability regions of laminated plates in the thermal environment. The effects of temperature, layer number, modulus ratio and load parameters on the dynamic instability of laminated plates are investigated. The results reveal that static and dynamic load, layer number, modulus ratio and uniform temperature rise have a significant influence on the thermal dynamic behavior of laminated plates.

• Journal of Korean Medical classics
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• v.21 no.4
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• pp.161-177
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• 2008

The oriental medicine is not static knowledge. Since the publication of "Hwangjenaegyeong(黃帝內經)", it has unceasingly changed and developed, that changes and developments ate the reflex of culture and civil progression, and they also are active acceptance of the time's demand. when we look back into the history of the Oriental medicine, angle of understanding disease can be classified into two concepts ; internal-external and Eumyang. Internal-external is whether we find the source of disease internal or external. Eumyang is deciding which is more important between yin-essence and yang-gi which are components of the essence-gi of body. This thesis view course of development of theory of the oriental medicine since publication of "Hwangjenaegyeong(黃帝內經)" at certain point of internal-external and Eumyang and with those as background, will inquire into sameness and difference between the Korean medicine and the Chinese medicine, and provide an advanced course for the oriental medicine.

• Journal of the Korea Furniture Society
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• v.26 no.4
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• pp.392-397
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• 2015

This study was carried out to analyze the effects on mechanical properties of deteriorated wood member by outdoor condition. The surface color, mechanical properties and structural stability of traditional wooden structures, exposed in water and UV, could be changeable. For the purpose, accelerated weathering test based on outdoor condition was carried out. The weathering time levels were composed 0, 500, 1000, 1,500 and 2,000 hours and mechanical properties were evaluated on each specimen according to weathering time level. Bending properties were decreased on weathering but recovered after 1,000 hours. Fatigue and impact strength were decreased to 1,000 hours and did not changed since then. Abrasion resistance was minimized in 1,000 hours. This results could be utilized for effectively stability management of traditional wooden structures and members.

• Journal of Microbiology
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• v.42 no.2
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• pp.94-98
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• 2004

Phenanthrene is a three-ring polycyclic aromatic hydrocarbon and commonly found as a pollutant in various environments. Degradation of phenanthrene by white rot fungus Trametes versicolor 951022 and its laccase, isolated in Korea, was investigated. After 36 h of incubation, about 46％ and 65％ of 100 mg/l of phenanthrene added in shaken and static fungal cultures were removed, respectively. Phenanthrene degradation was maximal at pH 6 and the optimal temperature for phenanthrene removal was 30$^{\circ}C$. Although the removal percentage of phenanthrene was highest (76.7％) at 10 mg/1 of phenanthrene concentration, the transformation rate was maximal (0.82 mg/h) at 100 mg/L of phenanthrene concentration in the fungal culture. When the purified laccase of T. versicolor 951022 reacted with phenanthrene, phenanthrene was not transformed. The addition of redox mediator, 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) or 1-hydroxybenzotriazole (HBT) to the reac-tion mixture increased oxidation of phenanthrene by laccase about 40％ and 30％, respectively.

• Mycobiology
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• v.46 no.4
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• pp.396-406
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• 2018

A newly isolated white rot fungal strain KU-RNW027 was identified as Trametes polyzona, based on an analysis of its morphological characteristics and phylogenetic data. Aeration and fungal morphology were important factors which drove strain KU-RNW027 to secrete two different ligninolytic enzymes as manganese peroxidase (MnP) and laccase. Highest activities of MnP and laccase were obtained in a continuous shaking culture at 8 and 47 times higher, respectively, than under static conditions. Strain KU-RNW027 existed as pellets and free form mycelial clumps in submerged cultivation with the pellet form producing more enzymes. Fungal biomass increased with increasing amounts of pellet inoculum while pellet diameter decreased. Strain KU-RNW027 formed terminal chlamydospore-like structures in cultures inoculated with 0.05 g/L as optimal pellet inoculum which resulted in highest enzyme production. Enzyme production efficiency of T. polyzona KU-RNW027 depended on fungal pellet morphology as size, porosity, and formation of chlamydospore-like structures.

• Korean Institute of Interior Design Journal
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• v.21 no.4
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• pp.77-86
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• 2012

The 5-day schoolweek system at the present has increased spare time of the youth. The society should use this opportunity to lead the youth to grow as a responsible community member as offering them programs to fulfill their desires and potential abilities. The purpose of this study is to have the improvements to give the youth appropriate and comfortable space. As the scope of the study, I selected a total of 5 places as each rating (the best, good, moderate, insufficient) 4 places in the comprehensive assessment conducted by youth facilities association among the independent buildings located in Seoul and 1 place after the assessment. As the methods of the study, with regard to environmental psychology and behavior and Youth cultural center, I investigated them theoretically as literature survey method, organized and analyzed, put them together through facilities research analysis and the survey. The research results are as follows : First, a static individual space should be placed in well accessibility due to high affinity for the facilities. Second, a dynamic individual space needs the place and equipment for physically and mentally healthy leisure culture. Third, a dynamic group space needs to provide ensuring service hours, setting proper size of space, comfortable indoor environment considering the relationship between physical activity and others. Fourth, a static group space needs to vary the programs to develop potential abilities by a variety of experiences to maintain the correlation the layout adjacent to leader's office.

• Journal of Digital Convergence
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• v.15 no.1
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• pp.497-507
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• 2017

The purpose of this study was to investigate the relationship among major satisfaction, career preparation behavior and career sense maturity of sports-related university students. To achieve the objective of this study 221 students in sports-related university were investigated with convenience sampling method. Frequency analysis, exploratory factor analysis, confirmatory factor analysis, correlations analysis and structural equation model analysis were executed using SPSS Statistics v21 and SPSS Amos v21. As the results, the following conclusion was derived. First, it was discovered that the major satisfaction had static influence to the career preparation behavior. Second, it showed that the major satisfaction gave static influence to the career sense maturity. Third, it showed that the career preparation behavior affected the career sense maturity.

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1430-1435
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• 2020

Bacterial cellulose (BC) has outstanding physical and chemical properties, including high crystallinity, moisture retention, and tensile strength. Currently, the major producer of BC is Komagataeibacter xylinus. However, due to limited tools of expression, this host is difficult to engineer metabolically to improve BC productivity. In this study, a regulated expression system for K. xylinus with synthetic ribosome binding site (RBS) was developed and used to engineer a BC biosynthesis pathway. A synthetic RBS library was constructed using green fluorescent protein (GFP) as a reporter, and three synthetic RBSs (R4, R15, and R6) with different strengths were successfully isolated by fluorescence-activated cell sorting (FACS). Using synthetic RBS, we optimized the expression of three homologous genes responsible for BC production, pgm, galU, and ndp, and thereby greatly increased it under both static and shaking culture conditions. The final titer of BC under static and shaking conditions was 5.28 and 3.67 g/l, respectively. Our findings demonstrate that reinforced metabolic flux towards BC through quantitative gene expression represents a practical strategy for the improvement of BC productivity.

• Cross-Cultural Studies
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• v.50
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• pp.453-482
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• 2018

This study is about the French preposition de. This preposition is considered to have no meaning in the French language, and to have only the grammatical function in its use. This study begins with doubts about such views regarding this preposition. We organize and classify general descriptions of the uses of this preposition. The uses are classified as 'expressing starting point,' 'giving characteristics' and 'performing grammatical functions' and this data is analyzed in terms of the associated cognitive grammar. In the case of expressing the starting point, it is assumed that the trajector moves from the starting point, to the destination point, in the domain of space and in the domain of time. Therefore, it is noted in this case that the preposition de has a dynamic concept. On the other hand, it is significant to understand that the preposition of the 'giving characteristics' defines an outline of a particular class by giving the class's characteristics. The preposition de has a static concept in this case. For the preposition de which introduces the infinitive, all of these dynamic and static concepts are faded and extremely grammaticalized.