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Structural Characteristics and Anti-inflammatory Activities of Chemically Sulfated-hyaluronic Acid from Streptococcus dysgalactiae (Streptococcus dysgalactiae로부터 분리된 히알루론산과 황화된 유도체의 구조와 항염증 활성)

  • Hong, Chang-Il;Jung, Eui-Gil;Han, Kook-Il;Kim, Yong Hyun;Lee, Sung Hee;Lee, Hong Sub;Han, Man-Deuk
    • Journal of Life Science
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    • v.26 no.5
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    • pp.545-554
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    • 2016
  • Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. HA is a natural and linear polymer composed of repeating disaccharide units of β-1, 3-N-acetyl glucosamine and β-1, 4-glucuronic acid. This work aimed to confirm the structural characteristics and anti-inflammatory activities of HA and its chemically sulfated-HA. HA was produced from a fed-batch fermentation process using Streptococcus dysgalactiae in a 5 l bioreactor. HA was isolated water-soluble form (HA-WS) and water-insoluble form (HA-WI) from culture medium, and was obtained chemically sulfated-derivative (S-HA) that resulted in a 90% yield from HA-WI. The structural features of the sulfated- HA (S-HA) were investigated by FT-IR and 1H-NMR spectroscopy. The FT-IR and NMR patterns revealed the similarity in both the FTIR spectrum as well as NMR spectrum of both reference standard and purified HA from S. dysgalactiae. The anti-inflammatory activities of HA and S-HA were examined on LPS-induced RAW 264.7 cells. S-HA was significantly inhibited production of pro-inflammatory mediators such as nitric oxide (NO) and PGE2 and the gene levels of iNOS and COX-2, which are responsible for the production of NO and PGE2, respectively. Furthermore, S-HA also suppressed the overproduction of pro-inflammatory cytokine TNF-α (<80 pg/ml) and IL-6 (<100 pg/ml) compared to that of HA-WI. The present study clearly demonstrates that HA-S exhibits anti-inflammatory activities in RAW 264.7 macrophage cells.

Quantitative Evaluation of Foodborne Pathogenic Bacteria in Commercial Sangshik (시판 생식에서 식중독균의 정량적 평가)

  • Kwak Hyo-Sun;Whang In-Kyun;Park Jong-Seok;Kim Mi-Gyeung;Lee Kyun-Young;Gho Young-Ho;Bae Yoon-Young;Moon Sung-Yang;Byun Ju-Sun;Kwon Ki-Sung;Woo Gun-Jo
    • Journal of Food Hygiene and Safety
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    • v.21 no.1
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    • pp.41-46
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    • 2006
  • This study was carried out to survey the prevalence of foodborne pathogens in Sangshik products and their raw materials far the purpose of ensuring safety of those products in market, and establishing microbial regulatory standard. From 2002 to 2004, a total of 191 Sangshik products were purchased from market or mail-order sales, and major foodborne pathogens; E. coli, Salmonella, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Listeria monocytogenes, Campylobacter jejuni, Yersinia enterocolitica, E. coli O157:H7, Vibrio parahaemolyticus were tested. B. cereus, C. perfringens and E. coli were detected from 29 samples (15.2%), 21 samples (11.0%) and 1 sample (0.5%), respectively. But other tested bacteria were not detected. For the identification of contamination source, 53 Sangshik ingredients were collected from 9 different manufacture factories. The results were similar with the Sangshik products. Aerobic plate counts were ranging from $1.0X10^3cfu/g\;to\;1.5X10^8cfu/g$. B. cereus was detected from 13 samples (24.5%), and counted as less than 100 cfu/g. C. perfringens were detected from 2 samples (3.8%), and counted as less than 100 cfu/g. Other foodborne pathogens were not detected except for B. cereus and C. perfringens. From the results, it was revealed that potential of microbial hazard by Sangshik was relatively low. However, it would be suggested that hygienic management and controling be needed for the prevention of growing contaminated pathogens and cross contamination during process and sale due to improper storage and management.

Prediction of field failure rate using data mining in the Automotive semiconductor (데이터 마이닝 기법을 이용한 차량용 반도체의 불량률 예측 연구)

  • Yun, Gyungsik;Jung, Hee-Won;Park, Seungbum
    • Journal of Technology Innovation
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    • v.26 no.3
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    • pp.37-68
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    • 2018
  • Since the 20th century, automobiles, which are the most common means of transportation, have been evolving as the use of electronic control devices and automotive semiconductors increases dramatically. Automotive semiconductors are a key component in automotive electronic control devices and are used to provide stability, efficiency of fuel use, and stability of operation to consumers. For example, automotive semiconductors include engines control, technologies for managing electric motors, transmission control units, hybrid vehicle control, start/stop systems, electronic motor control, automotive radar and LIDAR, smart head lamps, head-up displays, lane keeping systems. As such, semiconductors are being applied to almost all electronic control devices that make up an automobile, and they are creating more effects than simply combining mechanical devices. Since automotive semiconductors have a high data rate basically, a microprocessor unit is being used instead of a micro control unit. For example, semiconductors based on ARM processors are being used in telematics, audio/video multi-medias and navigation. Automotive semiconductors require characteristics such as high reliability, durability and long-term supply, considering the period of use of the automobile for more than 10 years. The reliability of automotive semiconductors is directly linked to the safety of automobiles. The semiconductor industry uses JEDEC and AEC standards to evaluate the reliability of automotive semiconductors. In addition, the life expectancy of the product is estimated at the early stage of development and at the early stage of mass production by using the reliability test method and results that are presented as standard in the automobile industry. However, there are limitations in predicting the failure rate caused by various parameters such as customer's various conditions of use and usage time. To overcome these limitations, much research has been done in academia and industry. Among them, researches using data mining techniques have been carried out in many semiconductor fields, but application and research on automotive semiconductors have not yet been studied. In this regard, this study investigates the relationship between data generated during semiconductor assembly and package test process by using data mining technique, and uses data mining technique suitable for predicting potential failure rate using customer bad data.

Effect of Bark and Drying Waste Liquor of Larix kaempferi Used as An Additive on The Fuel Characteristics of Wood Pellet Fabricated with Rigida Pine and Quercus mongolica Sawdust (첨가제로서 낙엽송의 수피 및 건조폐액이 리기다소나무 및 신갈나무 펠릿의 연료적 특성에 미치는 영향)

  • Yang, In;Chae, Hyun-Gyu;Han, Gyu-Seong
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.3
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    • pp.258-267
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    • 2017
  • In this study, pitch pine (Pinus rigida, PIR) and Mongolian oak (Quercus mongolica, QUM) pellets were fabricated with bark or/and drying waste liquor (DWL) of larch (Larix kaempferi, LAK) as an additive. Based on the results of fuel characteristics of the pellets, optimal conditions for producing the high-quality pellets were provided. In the analysis of chemical composition, bark contained holocelluose and lignin of 90% and over. DWL had 0.1% solid assumed to sugars which are generated from the oven-drying of LAK logs. QUM showed high ash content (2.2%) by containing of bark in the sawdust. Bark and DWL of LAK had high ash content of 4% and over. Calorific values of all specimens and additives were higher than that of the $1^{st}$-grade standard of wood pellets designated by NIFOS (18.0 MJ/kg). PIR and QUM pellets were fabricated with additive of 2 wt% based on the solid weight of oven-dried sawdust using a piston-type flat-die pelletizer, and thus ash content and calorific value of the pellets did not affect by the use of additive. Durability of the pellets increased with the use of additive. Durabilties of pellets, which were fabricated with bark as an additive and DWL as a controller of moisture content for sawdust, did not differ from those of pellets without additives and were lower than those of pellets either with bark or DWL. However, use of both bark and DWL for the production of wood pellets might be favorable because it can make a profit from the collection process of DWL. Based on the results of fuel characteristics of the pellets, QUM and PIR pellets were produced by a flat-die pelletizer. Moisture content (MC), bulk density and durability of the pellets improved with the use of additive. Particularly, sawdust MC of 10% and the addition of bark or DWL for PIR as well as sawdust MC of 12% and the addition of bark for QUM might be optimal conditions for the production of high-quality pellets. Except for the ash content of QUM pellets, other properties of PIR and QUM pellets exceeded the $1^{st}$-grade wood pellets standards of NIFOS.

A Comparison between BSCS's Guide and the Korean Curriculum for Developing Biological Literacy (생물학적 소양의 함양을 위한 BSCS 통합 권고안과 6,7차 교육과정 비교)

  • Koo, Soo-Jeong;Kim, Young-Shin;Kim, Byung-Suk;Lee, Sung-Jo;Chung, Wan-Ho
    • Journal of The Korean Association For Science Education
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    • v.20 no.3
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    • pp.396-410
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    • 2000
  • In this study, the concept presentation form, the content coherence of sub-dimensional concepts and the number of concepts of the 6th and the 7th Korean curriculum were analyzed comparing the guide to developing the secondary biology curricula to develop biological literacy with BSCS. According to the result, the discrimination between concept levels in the frame of contents of the Korean curricula is insufficient, because each of concepts presented in the knowledge domain as upper level and sub-dimensional concept elements as lower level are simply arrayed. Considering too much concepts of ecosystem, genetics, reproduction and metabolism, there should be an effort to reform the biological curriculum to include concepts evenly, not in the biased state, to reflect all the 6 unifying principles by BSCS for developing students' biological literacy. Finally there should be an effort to reflect the characteristics of each subjects concretely among Science 10, Biology I and Biology IT in the 7th curriculum considering the result that essential concepts to develop biological literacy are presented more in some principles of Biology II than Biology I. Thinking the results of the present study, concrete discussions should be made to set up the standard reference about biological literacy and to present essential concepts for teaching and learning to develop it in the process of biology textbook development for meeting the 7th Korean curriculum and in the development of 8th Korean curriculum in advance.

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The costume culture of China is as old and varied as her long history (중국 소수민족의 복식 연구(1))

  • 박춘순
    • Journal of the Korean Society of Costume
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    • v.26
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    • pp.175-206
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    • 1995
  • The costume culture of China is as old and varied as her long history. As China is a multiracial nation and consists of fifty-six min-ority races including Han race, there are not only fifty-six different costumes in China but each races' costume habit is very different. Therefore, Chinese penninsula can be considered an enor-mous exhibition center of the costumes. This study undertook on the assumption that the costumes' mainstream of Korea and east-northern Asia as well as that of China could be examined by investigating the minority races' costumes in the east-and west-northern areas of China. The process of evolution of the costume of a particular people, country or area is subject not only to constraints related to geography such as climate, topography or local products but is also affected by numorous environmental influences including cultural, economic, social and even pol-itical ones in terms of the selection of material, styling, color and standard of tailoring. In other words, things like philosophy of life, religious be-lief, aesthetic outlook, moral code, class system, degree of affluence, and cultural exchange will all be reflected directly or indirectly by features of a people's or country's style costume. Of course, there are several factors affecting to the style of costume of the minority people in China. However, the only three factors-geo-graphical and environmental, production method, and religious belef-will be touched in this study. First of all, the geograghical and eenviron-mental factor would be the decisive one because the costume should be designed to overcome the constraints of climate and geographical environ-ments. Accordingly, each race has an unique style of costume. The costume of the minority races in the northern parts are loose and wide, and made of warm furs. For instance, Mongolian robe has the quality of anti-wind, anti-cold and warmness, and the width of a sleeve is narrow and long. Secondly, the costume style can be said to be limited by the production pattern, when the geo-graphical environment was affected to decide the costume style, the production pattern was together affected to it . In case of Mongolian robe, they should satisfy the dual condition as the practical function. One is the condition that they should be fitted to the climate, and the other is the condition that they should be suit-able to the nomadic life. Mongolian robes are suitable to the nomadic peoples because they are designed for not only overcoming the cold wind and weather but being used as the bedquit at night. The costumes of Hoche people was made of the skin of the fish and wild animals because of their main means of living being fishing and hunting. Accordingly, their costumes are dur-able, warm and water-proof. Finally, the style of the costume is affected by the religious belief. In other words, the pattern in fashion is closely related with the religious be-lief or ancestor worship and nature worship. Ac-cordingly, the symbols of these worship are often emerged in the decoration of the costume. The design of costume of the people in the northern areas of China is very simple. It is related with their monotheism. On the other hand, the costumes of twen쇼 minority races in the east-northern parts of China can be devided into three racial groups such as the long robes of Man people and Mongols, Tunics of the peoples in the west-northern areas, and the pants and jackets of Hoche people. The minorority races all has not only the unique costume habit but their costumes are also related with their living style and production means.

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A Study on the Development of Aerobic Exercise Equipment Design for User-Centered -Focusing on Elliptical Cross Trainer- (사용자 중심의 유산소 운동기구 디자인 개발에 관한 연구 -Elliptical Cross Trainer를 중심으로-)

  • Chung, Kyung-Ryul;Song, Bok-Hee;Yoon, Se-Kyun;Park, Il-Woo
    • Archives of design research
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    • v.19 no.5 s.67
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    • pp.129-138
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    • 2006
  • It is expected that the typical lifestyle of the future will be transformed into an opulent and comfortable existence as the quality of life improves due to the increase in household income and reduction in working hours. In the meantime, as the standard of living becomes increasingly more comfortable and plentiful, the toll on physical health becomes magnified as a result of obesity and insufficient exercise caused by super nutrition and change in labor conditions (from physical labor to mental labor). This has instigated a deep awareness in fitness on the part of many people, forcing them to recognize the significance of daily exercise and physical activity. The high annual growth rate in the fitness and athletic apparatus market, which is more than 20%, is attributed to this phenomenon. The Elliptical Cross Trainer(ECT), which has drawn wide attention recently, is a non-impact athletic apparatus that not only promotes exercise of the upper body parts in such sports as skiing but also the exercise of lower parts of the body on a treadmill. It is a type of cross training athletic gear that has been developed for aerobic exercise throughout the entire body. It has already formed a market as big as that of the treadmill in Europe, America, etc. Recently, its demand is growing sharply in the Korean markets as well as those in Northeast Asian countries, Despite such demand increase and expansion, since most of the expensive ECTs are exclusively supplied by suppliers in only a few advanced countries, localization of the ECT is urgently required in order to enhance competitiveness of Korean manufacturers and to expand the market. This paper introduces the process and results of a design-engineering cooperative study that was peformed for the development of the ECT.

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SysML-Based System Modeling for Design of BIPV Electric Power Generation (건물일체형 태양광 시스템의 전력발전부 설계를 위한 SysML기반 시스템 모델링)

  • Lee, Seung-Joon;Lee, Jae-Chon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.10
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    • pp.578-589
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    • 2018
  • Building Integrated Photovoltaic (BIPV) system is a typical integrated system that simultaneously performs both building function and solar power generation function. To maximize its potential advantage, however, the solar photovoltaic power generation function must be integrated from the early conceptual design stage, and maximum power generation must be designed. To cope with such requirements, preliminary research on BIPV design process based on architectural design model and computer simulation results for improving solar power generation performance have been published. However, the requirements of the BIPV system have not been clearly identified and systematically reflected in the subsequent design. Moreover, no model has verified the power generation design. To solve these problems, we systematically model the requirements of BIPV system and study power generation design based on the system requirements model. Through the study, we consistently use the standard system modeling language, SysML. Specifically, stakeholder requirements were first identified from stakeholders and related BIPV standards. Then, based on the domain model, the design requirements of the BIPV system were derived at the system level, and the functional and physical architectures of the target system were created based on the system requirements. Finally, the power generation performance of the BIPV system was evaluated through a simulated SysML model (Parametric diagram). If the SysML system model developed herein can be reinforced by reflecting the conditions resulting from building design, it will open an opportunity to study and optimize the power generation in the BIPV system in an integrated fashion.

Characteristics of Melting Slag Using Sewage Sludge by Inorganic Additives (무기성 폐기물 첨가제 혼합에 따른 하수슬러지의 용융특성)

  • Bae, Jung-Suk;Lee, Sang-Won;Cha, Jae-Hwan;Kim, Hyo-Su;Kim, Chang-Won
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.11
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    • pp.1116-1122
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    • 2008
  • The existing technology which is for recycling aggregate using dried swage sludge have been limited for practical application, because the properties of aggregate are not regular and don't meet the recycling aggregate standard. In this research, an innovative slag-producing technology is developed by addition of oyster shell, waste cast-sand and iron-rust as inorganic waste additives. The mixed slag with the additives was evaluated at the various ratio of CaO/SiO$_2$, SiO$_2$/Al$_2$O$_3$ and Fe$_2$O$_3$/SiO$_2$. When the waste sludge was melted at 1,400$^{\circ}C$ during 20 minutes, the optimal ratio of CaO/SiO$_2$ for the slag added the oyster shell, SiO$_2$/Al$_2$O$_3$ for added the waste cast-sand and Fe$_2$O$_3$/SiO$_2$ for added the iron-rust were 1.00, 3.00 and 0.60, respectively. At the optimal condition, the bulk density of the slag was 2.24 g/cm$^3$, 2.45 g/cm$^3$ and 2.73 g/cm$^3$, And the 24 h water adsorption was 4.72%, 1.44% and 0.37%, respectively. Therefore it is proved that adding the waste additives to the process of the slag production contributed for elevation of recycling aggregate properties. And also it is expected that production cost can be reduced by decreased melting temperature.

Korean Art from the view of foreigners in Korea from the period of independence to 1950s (광복 후부터 1950년대까지 한국에서 활동한 외국인이 본 한국미술)

  • Cho, Eun-Jung
    • The Journal of Art Theory & Practice
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    • no.4
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    • pp.123-144
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    • 2006
  • Foreigners who arrived in Korea after the age of enlightenment were Japanese, Chinese and 'Westerners' who were Europeans and Americans. The westerners were diplomats who visited Korea for colonization or for increasing their economical profits by trading after the spread of imperialism, and tourists curious of back countries, artists, explores and missionaries to perform their roles for their religious beliefs. They contacted with Korean cultural and educational people as missionaries and instructors during Japanese colonial period. In 1945, the allied forces occupied Korea under the name of takeover of Japanese colony after Japan's surrender and the relation between foreigners and Korean cultured men enter upon a new phase. For 3 years, American soldiers enforced lots of systems in Korea and many pro-American people were educated. This relationship lasted even after the establishment of the government of Korean Republic and especially, diplomats called as pro-Korean group came again after Korean War. Among them, there were lots of foreigners interested in cultures and arts. In particular, government officials under American Forces who were influential on political circles or diplomats widened their insights toward Korean cultural assets and collected them a lot. Those who were in Korea from the period of independence to 1950s wrote their impressions about Korean cultural assets on newspapers or journals after visiting contemporary Korean exhibitions. Among them, A. J. McTaggart, Richard Hertz and the Hendersons were dominant. They thought the artists had great interests in compromising and uniting the Orient and the West based on their knowledge of Korean cultural assets and they advised. However, it was different from Korean artist's point of view that the foreigners thought Korean art adhered oriental features and contained western contents. From foreigners' point of view, it is hard to understand the attitude Korean artists chose to keep their self-respect through experiencing the Korean war. It is difficult to distinguish their thought about Korean art based on their exotic taste from the Korean artists' local and peninsular features under Japanese imperialism. We can see their thought about Korean art and their viewpoint toward the third world, after staying in Korea for a short period and being a member of the first world. The basic thing was that they could see the potentialities through the worldwide, beautiful Korean cultural assets and they thought it was important to start with traditions. It is an evidence showing Korean artists' pride in regard to the art culture through experiencing the infringement of their country. By writing about illuminating Korean art from the third party's view, foreigners represented their thoughts through it that their economical, military superiority goes with their cultural superiority. The Korean artist's thought of emphasizing Korean history and traditions, reexamining and using it as an original creation may have been inspired by westerners' writings. 'The establishment of national art' that Korean artists gave emphasis then, didn't only affect one of the reactions toward external impact, 'the adhesion of tradition'. In the process of introducing Korean contemporary art and national treasure in America, different view caused by role differences-foreigner as selector and Korean as assistant-showed the fact evidently that the standard of beauty differed between them. By emphasizing that the basis to classify Korean cultural assets is different from the neighborhood China and Japan, they tried to reflect their understanding that the feature of Korean art is on speciality other than universality. And this make us understand that even when Korean artists profess modernism, they stress that the roots are on Korean and oriental tradition. It was obviously a different thought from foreigners' view on Korean art that Korean artists' conception of modernism and traditional roots are inherent in Korean history. In 1950s, after the independence, Korea had different ideas from foreigners that abstract was to be learned from the west. Korea was enduring tough times with their artists' self-respect which made them think that they can learn the method, but the spirit of abstract is in the orient.

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