• Title/Summary/Keyword: Spot damage

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Occurrence of Apple Scar Skin viroid and Relative Quantity Analysis Using Real-time RT-PCR (Apple Scar Skin viroid 발생상황 및 Real-time RT-PCR을 이용한 상대정량 분석)

  • Kim, Dae-Hyun;Kim, Hyun-Ran;Heo, Seong;Kim, Se-Hee;Kim, Min-A;Shin, Il-Sheob;Kim, Jeong-Hee;Cho, Kang-Hee;Hwang, Jeong-Hwan
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.247-253
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    • 2010
  • Apple scar skin viroid (ASSVd) is one of the smallest viral pathogens infecting fruits, especially apple, and causes a significant damage to fruit trees. ASSVd usually induced the skin-dapple ring symptoms, but in 'Fuji' varieties, corked spot were occurred on the fruit skin in 2009. This new symptom will be of great helpful to diagnosis ASSVd in sight. ASSVd was surveyed in apple and pear from 2009 to 2010 in Korea, and ASSVd was identified in 20 out of 1,193 trees. The infection rate was 1.7%. To screen the infectivity of ASSVd among apple cultivars, real-time RT-PCR was applied followed by designing of ASSVd specific primers based on highly conserved regions of several ASSVd isolates including Korean isolate. NADH dehydrogenase subunit 5 (nad 5) gene, which is mRNA of the mitochondrial gene, was used for internal control. In this study, ASSVd infected apples were classified into 12 groups depending on different symptoms and symptom severity (scaring, rusting or malformation). Taken together, this study suggested that real-time PCR analysis was more sensitive to detect the low copy of ASSVd on early viroid infected apple skins than regular RT-PCR method.

Some Seaweed Deseases Occurred at Seaweed Farms along the South-Eastern Coast of Korea (동해남부연안 미역양식장의 병충해)

  • KANG Jae-Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.14 no.3
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    • pp.165-170
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    • 1981
  • Recently an unknown microbial desease and some parasitic crustaceans are prevailing in the sea-weed farms of Uudaria pinnatifida along the south-eastern coast of Korea.(1) Green spots probably caused by the microbial pathogens were found on the fronds of Undaira Pinnatifide. Particularly they were densely distributed on the distal half of the fronds. The tissues of the green spot area rot off, and small holes with green colored margin are formed. The holes at the distal part of the fronds are enlarged and they finally coalesced each other. Then this process accelerates decaying of the distal ends of the fronds.. The fronds growing in the central part of the farms are usually severely damaged, whereas in the marginal area of the farm toward the open sea side the damage is less serious. An examination revealed that the deseased fronds bore a number of viable bacteria, $6.8\times10^5\;to\;1.2\times10^6$ per gram at $15^{\circ}C$, whereas the healthy fronds $1.1\times10^4$. Twenty-six kinds of colonies, 247 strains of bacteria, were isolated from deseased fronds, belonging to Moraxella, Achromobacter, Vibrio, Flavobacterium, Acinetobacter, Pseudomonas, etc. (2) Pinholes occurred in one series on the frond. They were probably caused by a harpacticoid copepod, Thalestris sp. Seven years ago when the disease was first found to occur the copepod was observed on the fronds from March of the year. Recently, however, they have been found as early as December of the previous year. (3) A gammarid amphipod, Ceinina iaponica, invades the pith of the midrib through holdfast of thalli. This rarely causes the longitudinal seperation of the entire frond through the midrib as they bore a tunnel in the pith. Sometimes holdfasts of tile heavy damaged thalli make the frond departed from the substrate.

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Evaluation of the status of subgrade of high speed railway using HWAW method (HWAW방법을 이용한 고속철도 하부 노반 평가)

  • Park, Hyung-Choon;Park, Jin-O;Jin, Nam-Hui;Noh, Hee-Kwan;Bae, Hyun-Jung
    • Proceedings of the Korean Geotechical Society Conference
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    • 2010.03a
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    • pp.208-212
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    • 2010
  • The high-speed railway consists of tracks, gravel ballast and subgrade, and the dynamic load is passed to subgrade through track and gravel ballast. The relaxation condition of the gravel ballast is able to be evaluate relatively and to be repaired through a continuous management, but it is difficult to evaluate the condition of subgrade, which is final part of supporting dynamic load and to repair it when made a problem. The gravel ballast and subgrade are evaluated by determining shear wave velocity. To evaluate ballast and subgrade, a good method to determine shear wave velocity is a non-destructive experiment such as surface wave tests providing a prompt experiment because an experiment in railway has a lot of tests which are carried out following railway directions and needs to prevent damage of the system. In general, a railway has limitation of an experimental space by narrow width, sleeper and etc., and background noise by a reflector exists. The existing surface wave tests need a minimum space, and it is difficult to get a reliable test results on account of background noise effect. Therefore, it is difficult or impossible to apply to existing surface wave test of subgrade and ballast. In this study, the HWAW method is applied to determine a shear wave velocity profile of the underground. The HWAW method is the experiment which is able to be carried out on a narrow space, and it determines share wave velocity of a site by measuring the wave from surface sources on the same spot. In addition, it removes effects of background noise accordingly to a signal processing using harmonic wavelet transforms, so it is useful to evaluate subgrade of a high-speed railway in the narrow space and the situation of background noise. In order to check an application of the HWAW method, an experiment is carried out on a high-speed railway field and a test result is compared to boring results.

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Injury Aspects of the Stone Leek Leafminer, Liriomyza chinensis Kato (Diptera: Agromyzidae) on Welsh Onion (파를 가해하는 파굴파리의 충태별 피해 양상 및 행동)

  • 최인후;김정화;김길하;김철우
    • Korean journal of applied entomology
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    • v.42 no.4
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    • pp.335-343
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    • 2003
  • An experiment was conducted to investigate types of injury inflicted by the stone leek leafminer, Liriomyza chinensis Kato (Diptera: Agromyzidae) on welsh onion. A feeding scar made by an adult female was a hole round in shape, with diameter of 0.08 mm and 0.48 mm in lesion, resulting in a white spot, many of which often form vertical dotted lines on a leaf. Egg spots were oval with 0.1 ${\times}$0.14 mm in size, one or several of which often form a V-shape in group. Feeding by adults began immediately after emergence and was very active from 4th to 5th day. Oviposition was done from 2nd to 6th day after emergence. In both feeding and oviposition, they were more active in the day time. Larvae after emergence crawled up the leaf at first, and then moved up and down to feed on mesophyll. When in high density, they feed on leaf from leaf tip to bottom, and let the leaf die. Area of damage per one larva was calculated as 72.1 $\textrm{mm}^2$. The aged larvae escaped from the leaf in early morning, usually between 5 and 7 am. Most pupation sites were distributed near plants,5cm in soil depth and within 10 cm away from the plant. Pupae of L. chinensis overwintered 10cm below soil surface and emerged from early May to late June the next year Adults then moved to welsh onions near over wintering sites, nursery, transplanted, and levee.

Determination of Economic Injury Levels and Control Thresholds for Spodoptera exigua on Chinese Cabbage (배추 파밤나방의 경제적 피해수준 및 요방제 수준 설정)

  • Kim, Seon-Gon;Kim, Do-Ik;Ko, Suk-Ju;Kang, Beom-Ryong;Kim, Hong-Jae;Choi, Kyeong-Ju
    • Korean journal of applied entomology
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    • v.48 no.1
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    • pp.81-86
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    • 2009
  • Economic injury level and control thresholds for the management of beet army worm, Spodoptera exigua (Lepidoptera: Noctuidae) were evaluated on chinese cabbage of two different planting time. Two inoculation times were tested for each planting and the number of inoculated larva was 10, 20, 40, 80, respectively. Damages of leaves by first inoculation were 63.2% after eight days planting on 80 larva inoculation plot. By the second inoculation, those were below 50% after 20 days planting on the end of September. The linear relationships between population density and yield reduction were as following; Y = -10.62x + 867.9 ($R^2\;=\;0.643$) for 5 days and Y=-6.432x + 1074 ($R^2\;=\;0.720$) for 20 days. Based on these results the economic injury level was 5.4 larva for five days and 9.0 larva for 20 days per 20 chinese cabbage. The control thresholds calculated by 80% level of economic injury level were 4.3 and 7.2 larva, respectively.

In Vitro Screening of Antibacterial Agents for Suppression of Fire Blight Disease in Korea (기내 검정법을 이용한 국내 과수 화상병 방제제 선발)

  • Lee, Min Su;Lee, Ingyeong;Kim, Sam Kyu;Oh, Chang-Sik;Park, Duck Hwan
    • Research in Plant Disease
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    • v.24 no.1
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    • pp.41-51
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    • 2018
  • Since fire blight disease on apple and pear was produced in Korea in 2015, there were no registered chemicals to control against this disease. Instead, several antibacterial chemicals that were registered for other bacterial diseases such as soft rot and bacterial spot have been authorized by Rural Development Administration (RDA). However, these chemicals are not tested efficacy for fire blight disease except damage by those treatments on apple and pear in Korea. Thus, we evaluated efficiency using in vitro and in planta assays of antibacterial chemicals such as antibiotics and copper compounds including kasugamycin, oxytetracycline, oxolinic acid and streptomycin, and copper hydroxide, copper sulfate, oxine copper and tribasic copper sulfate, respectively. We also tested two kinds of biological agents. As expected, significant antibacterial effect was observed in vitro test of both antibiotics and copper-based chemicals. In planta test based on disease severity including ooze and water-soaked formation on immature pears, bacterial populations on blooms, and blight lesion formation in artificially inoculated shoots, kasugamycin, oxytetracycline and streptomycin have been shown the most efficiency among tested antibiotics. Four copper-based chemicals tested in this study, control effects are little bit lower than agricultural antibiotics but they seem to be available to use in terms of winter season. Biocontrol agents were also shown possibility to treat in eco-friendly farms. In addition, there are no antibiotic resistance genes in Korean isolates against antibiotics, which were selected for suppression of fire blight in this study.

A Study on the Culture and Clothing Behavior of Chunghahk Village (청학동의 문화와 의복행동에 관한 연구)

  • 이경화;한명숙
    • The Research Journal of the Costume Culture
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    • v.9 no.1
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    • pp.61-72
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    • 2001
  • Chunghahk-dong is located in a retired spot, Jiri Mountain and has been built up its won religion and culture without any exchange outside world. People in Chunghahk-dong believe in a religion called Genjungyutaosim. Forming a trinity on which mind is Taoism, body is Buddhism, and behavior is confucianism. These are the principle elements to form a village. They made their own unique cultural factor such as longhaired boys and girls, wearing traditional cloths and hat, a monastic life, folk mores and family rituals, and private school system. This shows a great deal of affection on their life style and organization and is closely connected with community and culture. Grasping a culture and behavior on clothing, it has propose to understand Chunghahk-dong properly according to this study. A method of study has a purpose to understand culture and clothing behavior precisely in Chunghahk-dong. I inquire ito clothing behavior with investigation and study of picture script. The results are as followed; People in Chunghahk-dong show unique clothing and hair style behavior based on Genjungyutao. First, men and women wear a white cloth called HanBok(Korean costume) and footwear made of rubber. Only men wear a blue vest. A grown up men wear a long coat when they go out. They are dressed in tractional cloth for a ceremonial occasion. The reason why they put on the HanBok(Korean costume) is a belief on our traditional cloth becomes a standard dress in the future. They believe Korea becomes a standard dress in the future. They believe Korea becomes a standard dress in the future. They believe Korea become a leading country in the world. This clothing behavior is symbol of Genjungyutao and has a role of delivery system for expressing their split. Second, In sign of being a Genjungyutao men, they let their grow hair. Cutting hair is not to be a Genjungyutao men any more. There is few reason that people in Chunghahk-dong let grow their hair : First, because of importance in TanGun's ideology, they believe Korea is a first nation in the world and can't cut their hair which has grown from TanGun (The founding father of the Koran nation) Chosun traditionally. Second, Cutting their hair runs counter to the principle of nature. Third, They value their body for confucianism. They don't want to damage their body because of body from their parents. Boys and girls braid and tie up their hair and adult tie a topknot and a chignon. Wearing a YouGun(A horsehair skullcap) at home and Kat(A Korean top hat) when going out, they express as a Genjungyutao. Hemp cloth and synthetic fibers are main clothes. Also, they wear an improved HanBok(Korean costume) these days on influence outside.

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A Study on Procurement of Construction Materials in the HwaSeong Construction Project (화성건설공사에서 자재의 조달방법 연구)

  • Kim, Kyoon-Tai
    • Journal of the Korea Institute of Building Construction
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    • v.9 no.6
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    • pp.183-188
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    • 2009
  • HwaSeong (Suwon Fortress) was built during the reign of King Jeong-jo in the Choseon Dynasty. Detailed information on the construction of HwaSeong can be found in Hwaseong Seongyeok Euigui. In this sense, it is necessary to perform a comparative analysis on the Hwaseong Seongyeok Euigui based on construction management factors. This paper aims to analyze the HwaSeong construction project from a modern construction perspective by comparing and reviewing information related to the procurement of construction materials of the time. For this, wooden and steel materials and tiles were analyzed by supply area, supply channel, type and price. From the results of the analysis, it is found that the government used both private and official channels to supply materials. In particular, except for wooden materials that could be supplied from Bongsan, the supply of materials showed a high dependency on private channels. In terms of steel, it was almost 30 percent cheaper through private channels than when purchased through the official channel. Finally, materials vulnerable to damage like tiles were manufactured from the areas near the construction site. From this fact it can be interpreted that the government had a flexible procurement system, including manufacturing on the spot and external suppliers,depending on the property of the material. It is expected these analytical results will serve as key data to understand the management factors in constructing HwaSeong.

Counter Measures of the Subway Terrorism through Case Analysis (사례분석을 통한 지하철 테러에 대한 대책)

  • Kwon, Jeong-Hoon;Kim, Tae-Hwan;Choi, Jong-Gyun
    • Korean Security Journal
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    • no.18
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    • pp.1-20
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    • 2009
  • Nowadays most nations around the world including Korea have experienced absolute shortages of available urban space. To solve various problems of the city, each nation constantly tends to extend the underground space. However there is a serious problem in making use of the underground space. Especially new terrorism coming into existence after 9.11 terror turns into the so-called ‘soft target’ which has something to do with public transportation facilities available to most people. Good examples are like these: poisonous gas attacks in Tokyo subway in 1995, Daegu subway station fire in 2003, serial bomb blast of London subway in 2005. In spite of being a concern on incidents related to the underground space it is inevitable to utilize the underground space and the tendency is growing. But Korea lags badly behind in foreign countries in this field and so seeking measures is urgently needed. Therefore the aim of this study is to note visible damages stemmed from the domestic and foreign underground space and propose more effective and adequate measures. Safety measures of terrorism are associated to minimize damage out of terrorism and they are as follows. In the first place, preparing protective equipment for saving a life from fire attacks and poisonous gas is needed urgently. In the second place, counterpart management on the spot and systematic security training should be established in order to minimize injury. In the third place, fire escapes must be provided for a rapid evacuation of potential unspecified individuals. In the fourth place, building up a network of related institutions is required for a systematic omnidirectional counterpart. Finally the Korean government ought to take fast and appropriate actions for the injured and bereaved family of the terror incident.

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A Fundamental Study for the Automatic Control System in Greenhouse Using Microcomputer(III) -A variation of temperature and humidity by the window opening ways of the even-Span type house- (마이크로컴퓨터에 의한 시설재배의 자동화에 관한 기초연구(III) -양지붕형 하우스의 창 개방방법에 따른 온.습도의 변화-)

  • 김진현;김철수;구건효;이기명
    • Journal of Biosystems Engineering
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    • v.20 no.2
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    • pp.162-172
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    • 1995
  • The ventilation in greenhouse have been important for such as adjustment of temperature, supplying of the oxygen, prevention of the overhumidity, density adjustment of $CO_2$, discharge of harmfulness gas, etc. However, the general ventilation which had been used the quantitative control method in discharge of a property of air mechanism in greenhouse, and caused mainly in waste of the heating energy and growth obstacle of the vegetable. Therefore, this study was peformed to obtain more scientific ventilation method using by analysis and measurement of the isothermal lines according to opening of window ventilation in greenhouse, and the results are summarized as follows. 1. The ventilating amount was more influenced by rather opening amount of window than the ventilating time. 2. In window ventilation, the temperature in greenhouse was mostly changed within 5 minutes after ventilating not regard to the spot of opening, after about 10 minutes temperature became to equilibrium state under the respective ventilating conditions. 3. In opening of the skylight only, isothermal lines were complicated, therefore, a tall vegetable may be possible to damage by a cold-weather from the lower central port in greenhouse. 4. Isothermal lines were a tendency to simply in opening of a side window that may be more effective ventilation in kinds of the short vegetable. 5. In conditions of internal temperature>setting temperature>external temperature, a skylight can be suitable to open 10~20cm in order to the optimum ventilation in greenhouse. 6. In conditions of internal temperature>external temperature>setting temperature, opening of all the windows or both the side windows that can be suitable in order to obtain the optimum ventilation in greenhouse. 7. An effect of ventilation was the most excellent to open of all the windows or both the side windows, and it were also found orderly excellent to open of the side window and the skylight or the skylight only, to open of the side window only. 8. Temperature was varied as the equation of T=Tc+ (To-Tc)e-at, and the ranges of (a) values were limited within 0.34~0.68. 9. A variations of humidity were similar to that of temperature, s.

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