• Molecular & Cellular Toxicology
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• 제4권1호
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• pp.11-15
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• 2008

The expression of neuronal nitric oxide synthase (nNOS) is regulated by various spliced first exons (exon 1a-1i), sharing differentially common exon 2 in diverse human tissues. The highly complex structure and regulation of human nNOS gene gave limitations of information for the precise mechanism of nNOS regulation. In the present study, we report that the repeats of polymorphic dinucleotides $(GT)^nA(TG)^n$ repeats located in just upstream to the exon 1f in human nNOS gene play suppressive role in transcription, as shown in the characteristics of Z-DNA motif in other genes. In neuronal and trophoblast cells transfected transiently with luciferase construct without dinucleotide repeats at the 5'-flanking region of exon 1f in nNOS gene, the luciferase activity was increased markedly. However, the presence of the dinucleotide repeats dramatically suppressed the luciferase activity to the basal level, and which was dependent on the length of $(GT)^n$ and $(TG)^n$ repeats. More importantly, we found the polymorphisms in the length of dinucleotide repeats in human. Furthermore, we show for the first time here that there is a significant association of the lengths of polymorphic dinucleotide $(GT)^n$ and $(TG)^n$ repeats with the risk of schizophrenia.

• 한국광학회지
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• 제21권5호
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• pp.200-205
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• 2010

본 논문에서는 포토닉 밴드갭 광섬유(Photonic Bandgap Fiber: PBGF) 사이에 중공광섬유(Hollow Optical Fiber: HOF)를 융착 접속시켜 만든 광섬유 간섭계와 넓은 모드 면적을 가지는 광자결정 광섬유(Large Mode Area-Photonic Crystal Fiber: LMA-PCF)사이에 HOF를 융착접속시켜 만든 광섬유 간섭계의 온도 및 스트레인에 대한 광학적 특성을 분석하였다. PBGF 또는 LMA-PCF와 HOF의 융착접속시 광섬유내 공기구멍을 최대한 유지하도록 융착조건을 최적화하여 접속 손실을 줄였다. PBGF와 HOF로 구성된 광섬유 간섭계의 온도 및 스트레인에 대한 민감도는 각각 15.4 pm/$^{\circ}C$와 0.24 pm/${\mu}\varepsilon$으로 측정되었으며, LMA-PCF와 HOF로 구성된 광섬유 간섭계의 온도 및 스트레인에 대한 민감도는 각각 17.4 pm/$^{\circ}C$와 0.2 pm/${\mu}\varepsilon$으로 측정되었다.

• BMB Reports
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• 제31권4호
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• pp.413-417
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• 1998

Transgenic mice containing a bovine ${\beta}-Casein/Human$ lysozyme fusion gene (pBZ) were generated in order to produce human lysozyme in their milk. The expression vector was a quadripartite fusion consisting of a 2 kb upstream DNA of the bovine ${\beta}-casein$ gene, human lysozyme gene, intron II of the rabbit ${\beta}-globin$ gene, and the polyadenylation/termination signals of SV40 DNA. Fertilized mouse zygotes were microinjected with pBZ, then transferred into the oviduct of foster mothers. Out of 20 mice born, 11 survived until postweaning and three were identified as positivetransgenic by Southern blot analysis (one male and two females). The founder mice were mated to BCFl mice to produce transgenic progeny. It was confirmed by RT-PCR and Northern blot analyses that the transgene was specifically expressed in the mammary gland of the founder mice. Furthermore, the artificial introns within the transgenic RNA was proven to be correctly spliced out as judged by RT-PCR analysis. These results indicated that transgenic mice generated in this study properly expressed the human lysozyme RNA in their mammary gland.

• Journal of Animal Science and Technology
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• 제60권10호
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• pp.25.1-25.10
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• 2018

The central dogma of gene expression propounds that DNA is transcribed to mRNA and finally gets translated into protein. Only 2-3% of the genomic DNA is transcribed to protein-coding mRNA. Interestingly, only a further minuscule part of genomic DNA encodes for long non-coding RNAs (lncRNAs) which are characteristically more than 200 nucleotides long and can be transcribed from both protein-coding (e.g. H19 and TUG1) as well as non-coding DNA by RNA polymerase II. The lncRNAs do not have open reading frames (with some exceptions), 3`-untranslated regions (3'-UTRs) and necessarily these RNAs lack any translation-termination regions, however, these can be spliced, capped and polyadenylated as mRNA molecules. The flexibility of lncRNAs confers them specific 3D-conformations that eventually enable the lncRNAs to interact with proteins, DNA or other RNA molecules via base pairing or by forming networks. The lncRNAs play a major role in gene regulation, cell differentiation, cancer cell invasion and metastasis and chromatin remodeling. Deregulation of lncRNA is also responsible for numerous diseases in mammals. Various studies have revealed their significance as biomarkers for prognosis and diagnosis of cancer. The aim of this review is to overview the salient features, evolution, biogenesis and biological importance of these molecules in the mammalian system.

• Computers and Concrete
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• 제12권2호
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• pp.131-149
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• 2013

Various types of reinforcement splicing methods have been developed and implemented in reinforced concrete construction projects for achieving the continuity of reinforcements. Due to the complicated reinforcement arrangements and the difficulties in securing bar spacing, the traditional lap splicing method, which has been widely used in reinforced concrete constructions, often shows low constructability and difficulties in quality control. Also, lap spliced regions are likely to be over-reinforced, which may not be desirable in seismic design. On the other hand, mechanical splicing methods can offer simple and clear arrangements of reinforcement. In order to utilize the couplers for the ribbed-deformed bars, however, additional screw processing at the ends of reinforcing bars is typically required, which often lead to performance degradations of reinforced concrete members due to the lack of workmanship in screw processing or in adjusting the length of reinforcing bars. On the contrary, the use of screw-ribbed reinforcements can easily solve these issues on the mechanical splicing methods, because it does not require the screw process on the bar. In this study, the mechanical coupler suitable for the screw-ribbed reinforcements has been developed, in which any gap between the reinforcements and sleeve device can be removed by grouting high-flow inorganic mortar. This study presents the uniaxial tension tests on the screw-ribbed reinforcement with the mechanical sleeve devices and the cyclic loading tests on RC columns with the developed coupler. The test results show that the mechanical sleeve connection developed in this study has an excellent splicing performance, and that it is applicable to reinforced concrete columns with a proper confinement by hoop reinforcement.

• 한국콘텐츠학회논문지
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• 제10권10호
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• pp.102-110
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• 2010

선택 스플라이싱은 유전자 발현의 중요한 과정 중 하나이다. 선택 스플라이싱이 발생함에 따라, 돌연변가 발생하여, 질병을 일으킬 수 있다. 대부분의 선택 스플라이싱 연구는 EST(Expressed Sequence Tag)를 이용한다. 그러나, EST를 이용하여 선택 스플라싱을 예측하는 데는 몇 가지 단점이 있다. EST가 저장되어 있는 라이브러리가 잘 정돈되어 있지 않거나, 잘못 열거되어 있을 경우, 실험 시 EST를 잘못 선택할 수 있다. 또한, EST가 아직 발견되지 않은 유전 서열에서는 선택 스플라이싱을 찾을 방법이 없다. 이 논문에서는 이러한 EST 기반 연구의 약점을 개선하고, 선택 스플라이싱의 탐지 및 예측의 질을 높이기 위해서, pre-mRNA에서 One-leaf One-node Tree 알고리즘을 제안한다. 이 트리는 Arabidopsis thaliana의 각 염색체에 대해서 실험되었다. 실험 결과, 모든 염색체에서 codons에 따라 일반 스플라싱과 선택 스플라싱이 다른 패턴을 가지는 것으로 나타났다. 트리 알고리즘에서 도출된 패턴으로 부터, 아직 발견되지 않은 선택 스플라싱도 예측할 수 있다.

• 한국정보통신학회논문지
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• 제20권4호
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• pp.833-839
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• 2016

본 논문에서는 효율적인 접합 영상 검출을 위한 마르코프 천이 및 동시발생 확률에 대한 선택적 특징 추출 방법을 제안한다. 제안하는 방법에서는 이산 코사인 변환 영역에서 블록간 계수의 차이를 이용하여 특징들을 구성하고, 특징들의 각 위치에서 원 영상과 접합영상의 특징 분포의 상이성을 확인하기 위해 Kullback-Leibler 수렴값을 구한다. 이를 바탕으로, 마르코프 확률 특징과 동시발생 확률 특징 가운데 해당 위치에서 가장 큰 차이값을 갖는 특징을 선택하여 최종 특징으로 선택하고, SVM 분류기를 이용하여 학습 및 테스트한 후 그 유효성을 판별한다. 실험 결과를 바탕으로 제안하는 방법이 기존의 방법보다 제한된 특징수로 높은 영상접합 조작 결과를 보임을 확인하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제17권4호
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• pp.259-265
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• 2013

The anti-apoptotic effect of (-)-epigallocatechin-3-gallate (EGCG) during unilateral testicular torsion and detorsion (TT/D) was established in our previous study. In mice, the smallest inhibitor of apoptosis, survivin, is alternatively spliced into three variants, each suggested to have a unique function. Here, we assessed how EGCG exerts its protective effect through the expression of the different survivin splice variants and determined its effect on the morphology of the seminiferous tubules during TT/D. Three mouse groups were used: sham, TT/D+vehicle and TT/D treated with EGCG. The expression of the survivin variants (140 and 40) and other apoptosis genes (p53, Bax and Bcl-2) was measured with semi-quantitative RT-PCR. Histological analysis was performed to assess DNA fragmentation, damage to spermatogenesis and morphometric changes in the seminiferous tubules. In the TT/D+vehicle group, survivin 140 expression was markedly decreased, whereas survivin 40 expression was not significantly different. In parallel, there was an increase in the mRNA level of p53 and the Bax to Bcl-2 ratio in support of apoptosis induction. Histological analyses revealed increased DNA fragmentation and increased damage to spermatogenesis associated with decreased seminiferous tubular diameter and decreased germinal epithelial cell thickness in the TT/D+vehicle group. These changes were reversed to almost sham levels upon EGCG treatment. Our data indicate that EGCG protects the testis from TT/D-induced damage by protecting the morphology of the seminiferous tubules and modulating survivin 140 expression.

• 약학회지
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• 제52권1호
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• pp.73-78
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• 2008

Immune system can protect host attacking from a variety of microorganism and virus through innate and adaptive immunities. The innate immune system can be activated by recognition of conserved carbohydrates on the cell surface of pathogen resulting in protection, immunity regulation and inflammation. Immunostimulating and anti-tumor ${\beta}$-glucan, major cell wall component of many fungi, could be recognized as pathogen associated molecular pattern (PAMP) by C-type lectin such as pathogen recognition receptor (PRR) of host innate immunity cells. In spite of many studies of basidiomycetes ${\beta}$-glucan on immunostimulation, little is known about the precise mechanism as molecular-level. Among C-type lectins, dectin-1 was cloned and reported as a ${\beta}$-glucan receptor. In this report, we demonstrated induction of cytokine gene transcription by Ganoderma lucidum ${\beta}$-glucan in the absence or presence of lipopolysaccharide (LPS) by RT-PCR analysis. The expression of murine dectin-1 (MD-1) on RAW264.7 macrophage by RT-PCR showing both the full length, 757 bp $(MD-1{\alpha})$ and alternative spliced form, 620 bp $(MD-1{\beta})$. Both $MD-1{\alpha}$ and $MD-1{\beta}$ mRNAs were induced by ${\beta $-glucan both in the absence and presence of LPS. To explore expression of inflammatory cytokines by ${\beta}$-glucan, RAW264.7 cells were treated with ${\beta}$-glucan for 12 hours. As a result, the expressions of IL-1 IL-6, IL-l0 and $TNF-{\alpha}$ were increased by ${\beta}$-glucan treatment in a dose-dependent fashion. From these results, ${\beta}$-glucan induced transcriptions of dectin-1 and immune activating cytokine genes, indicating induction of immune allertness by expressing dectin-1 and secreting inflammatory cytokines.

• 전자공학회논문지
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• 제51권5호
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• pp.160-167
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• 2014

본 논문에서는 밴포드 법칙과 컬러의 차이를 이용한 영상 접합 조작 검출 방법을 제안하고자 한다. 조작이 의심되는 영상에 대하여 먼저 컬러 변환을 시행한 후, 이산 웨이블릿 변환 및 이산 코사인 변환을 수행한다. 이상적인 밴포드 분포와 의심되는 영상에 대한 밴포드 분포의 차이를 특징으로 추출한다. 아울러 컬러 성분에 대한 밴포드 분포의 차이를 특징으로 사용한다. 본 논문의 방법은 13개의 특징만으로 우수한 접합 영상 검출 성능을 보인다. 추출된 특징 벡터를 SVM(support vector machine) 분류기를 이용하여 학습한 후 영상의 접합 여부를 판별한다. 본 논문의 방법은 기존의 방법보다 적은 수의 특징으로 높은 영상 접합 조작 결과를 보임을 확인하였다.