Park, Ji-Eun;Jeong, Yeon Jae;Kim, Hye Young;Yoo, Young Hyun;Lee, Kwang Sik;Yang, Won Tae;Kim, Doh Hoon;Kim, Jong-Min
Development and Reproduction
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v.24
no.3
/
pp.231-239
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2020
Many benefits of silk protein fibroin (SPF) have been suggested in biomedical applications; and notably, significant SPF effects have been observed for metabolic syndromes that are directly linked to insulin resistance, such as type 2 diabetes mellitus (T2DM). Based on our previous findings, we believe that SPF from spiders exhibits outstanding glucose-lowering effects in diabetic BKS.Cg-m+/+Leprdb mice. In order to evaluate the dietary effects of SPF in diabetic animals, we generated several lines of transgenic rice (TR) that expresses SPF, and the feeding of TR-SPF to diabetic animals decreased blood glucose levels, but did not change insulin levels. Western blot analyses of hepatic proteins showed that AMP-activated protein kinase (AMPK) expression and phosphorylation both decreased in TR-SPF-fed groups, compared with controls. This finding suggests that the glucose-lowering effects in this diabetic animal model might be AMPK-independent. In contrast, six-transmembrane protein of prostate 2 (STAMP2) was upregulated after TR-SPF exposure. Together with STAMP2, the Akt protein phosphorylation increased after TR-SPF exposure, which indicates that STAMP2 leads to Akt phosphorylation and thus increases insulin sensitivity in hepatocytes. Importantly, the hepatic steatosis that was seen in the liver of diabetic mice was remarkably alleviated in TR-SPF-fed mice. Hepatocytes that were immunopositive for STAMP2 were overwhelmingly observed in hepatic tissues from TR-SPF-fed mice compared to the control. Taken together, these results suggest that feeding diabetic mice with TR-SPF upregulates STAMP2 expression and increases Akt phosphorylation in hepatic tissues and thus potentially alleviates insulin resistance and hepatic steatosis.
Na, Young-Wang;Jeong, Ho Jeong;Cheong, Jae Woan;Choi, Hyo Gil;Jeon, Heung Young;Kim, Do Sun;Rho, Il Rae
Horticultural Science & Technology
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v.31
no.5
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pp.648-651
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2013
A new cultivar 'Daewang' strawberry was developed by the National Institute of Horticultural & Herbal Science for forcing culture in 2010. The cultivar 'Daewang' was originated from the cross between 'Maehyang', a high firmness cultivar and 'Wongyo 3111', a high sugar content in 2006. The cultivar shows erect plant type, vigorous growth habit, early flower bud differentiation with 12-15 flowers per cluster from planting on healthy nursery. Fruits of 'Daewang' are conical type having a bright red skin color, and 16-17 g in an average weight. 'Daewang' is suitable for forcing culture as time required for dormancy breaking ranged between 50 and 100 hours. This cultivar has excellent taste for high sugar/acid ratio as sugar content of $11.1^{\circ}Brix$, acidity of 0.39% with abundant texture and can be harvested by late spring because the fruit firmness of 'Daewang' was $18.2g{\cdot}mm^{-2}$ that was about $7.9g{\cdot}mm^{-2}$ higher than $10.3g{\cdot}mm^{-2}$ of 'Akihime' cultivar. But although total yield is not significantly different from 'Akihime' cultivar, its marketable yield is remarkably higher than that of 'Akihime' cultivar. Disease and pest resistance of 'Daewang' have a tendency to sensitive powdery mildew, anthracnose and spotted spider.
A fixed-precision-level sampling plan was developed to establish control of the two-spotted spider mite, Tetranychus urticae, in two strawberry greenhouses (conventional plot, natural enemy plot). T. urticae was sampled by taking a three-leaflet leaf (1 stalk) from each plant (3 three-leaflet leaves) from each sampling position. Each leaflet was divided into three different units (1-leaflet, 2-leaflet, and 3-leaflet units) to compare relative net precision (RNP) values for selection of the appropriate sampling unit. The relative net precision values indicated that a 1-leaflet unit was more precise and cost-efficient than other units. The spatial distribution analysis was performed using Taylor's power law (TPL). Homogeneity of the TPL parameters in each greenhouse was evaluated by using the analysis of covariance (ANCOVA). A fixed-precision-level sequential sampling plan was developed using the parameters of TPL generated from the combined data of the conventional plot and natural enemy plot in a 1-leaflet sampling unit. Sequential classification sampling plans were also developed using the action threshold of 3 and 10 mites for pooled data. Using the results obtained in the independent data, simulated validation of the developed sampling plan by Resampling validation for sampling plan (RVSP) indicated a reasonable level of precision.
This study explores how folktales' motifs are used in diverse cultural contents such as literature, movies, or comics. More precisely, it is concerned with mysterious characters like Babayaga and Koshchei who appear in Russian fairytales. When the motifs of the reviewed literature are analyzed, the following three criteria are used for classification: New Writing, Rewriting, and New Version. Or course, it is New Writing that is far from the original work. Most of the collected works, including Joan Aiken's The Kingdom Under the Sea, Patricia Polacco's Babushka's Doll, Babushka Baba Yaga, and Tai?-Marc Le Thanh's BABAYAGA, belong to New Writing. The New Writing type mainly focuses on Babayaga's secluded life. In fairytales, Babayaga is depicted as the goddess of Mother Earth, the heroine of the Nature, or the ruler of the Animal Kingdom. That is why she lives in the deep and uninhabited wood. She is related to the Coming of Age ceremony. Her 'Hut on hen's legs' is an obstacle for immature protagonists to face before they come of age. Protagonists are supposed to solve the problems posed by Babayaga. If they succeed, they can get her magical help so as to complete their final mission. Babayaga is likely to appear as their antagonist. Protagonist may be subjected to a terrible ordeal created by Babayaga, and then come of age by recovering from that ordeal. Sometimes, Babayaga helps protagonists to get a grip on reality. That is why she lives in a hut in the deep and dark forest which is on the borderline between life and death. On the other hand, Marianna Mayer's Baba Yaga and Vasilisa the Brave has been classified as a controversial work between New Writing and Rewriting. It is apparently closer to New Version. However, it cannot be classified as a work of New Version because the author incorporates 'probability' into her work. Among the reviewed works, it is Korean Writer Rye, Kami's The Spider Woman's House that best reflects the essence of Babayaga. Babayaga and other characters are amusing in movies because most of the movies are animations for children. In one sense, it is positive that the scary characters in folktales are approachable to children. In other sense, however, it is regrettable that symbolic motifs are completely eliminated. In Mike Mignola's Hellboy, Japanese animations such as Index or Narutaru, on-line games such as Cabal or Vampire: The masquerade, the essence of the supernatural characters in folktales is completely eliminated, and only their 'belligerent power' stands out. It is desirable to put stories into perspective whether they are written or told. The literature property of folktales provides a special opportunity to readers. In this light, a variety of motifs have a good reason to be reborn as modern cultural contents. The bottom line is to maintain its true nature.
Rho, Il Rae;Cho, Yong Seop;Cheong, Jae Wan;Jeong, Ho Jeong
Korean Journal of Breeding Science
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v.41
no.3
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pp.319-323
/
2009
Strawberry is an out-crossed crop that is relatively sensitive to inbreeding, and as a result, most of breeding programmes in strawberry have been based on selection breeding where elite parents are selected from intercrossing. Also, pedigree breeding has been successfully employed in a number of instances to concentrate genes of interest. Therefore, in order to obtain inbred line having the genotype of excellent combining ability by selfing and incrossing to improve breeding efficiency of strawberry, elite varieties were self-pollinated. From the selfing line of "Benihoppe" cultivar, we selected "Wongyo 3111" having excellent horticultural traits such as plant vigor, sugar content and fruit hardness among these progenies. "Wongyo 3111" shows erect plant type, vigorous growth habit, early flower bud differentiation with 16~17 flowers per cluster. Fruits of "Wongyo 3111" are conical type having a bright red skin color, and 17.2 g in an average weight. Also that have a relatively more high sugar/acid ratio and firmness than check cultivars as sugar content of 11.3oBx, acidity of 0.50% and firmness of 21.7 g/$mm^2$. But although yield of "Wongyo 3111" is not significantly different from check cultivars, its marketable yield is remarkably lower than that of check cultivars because of poor fruit-setting under low temperature. Disease and pest reactions of "Wongyo 3111" are more sensitive to powery mildew, anthracnose and spotted spider mites than check cultivars. Therefore, "Wongyo 3111" is suitable for intermediate parent line better than as a cultivation cultivar.
Methanol and hexane extracts from 35 species in 27 families of herbal plants were evaluated for their acaricidal activities against two-spotted spider mite, Tetranychus urticae by leaf-dipping and spraying methods in laboratory, pot and field, respectively. Acaricidal activities were different depending on herbal plants. When T. urticae was released on leaves of Phaseolus vulgaris var. humilis that were dipped in the 1,000 ppm hexane extracts from fruits of Torreya nucifer and Daphan genkwa, seeds of Xanthium strumarium and Pharbitis nil at one minute they experienced 56.8, 47.8, 47.7, and 47.7% mortalities, respectively. Dipping time influenced acaricidal activity, that is, one minute dipping was more effective than 30 second dipping of Phaseolus vulgaris var. humilis leaves. However, extracts of X. strumarium and P. nil seeds had comparatively high activities. Concentration of hexane extract of X. strumarium and P. nil seeds revealing LC50 were 1,824 and 1,899 ppm, respectively. Extracts of both plants from hot and cold water were not effective representing <20% mortality at 1,000 ppm. However, hexane extracts of X. strumarium and P. nil seeds were effective against T. urticae on P. vulgaris var. humilis representing 76.3 and 71.3% mortalities in pot, respectively. Control effects of hexane extract of P. nil seed were 50.8 and 35.1% at 2,000 and 1,000 ppm against T. urticae on Chrysanthemum morifolium in greenhouse, respectively.
The Jurassic granitic rocks in the area of Gwangdeoksan located along the boundary between Hwacheon and Cherwon in northern Gyeonggi Massif consist of two-mica granite, garnet-bearing two-mica granite, mica-granite, and porphyritic biotite granite. These granitic rocks are calc-alkaline series and plotted in peraluminious domain in A/CNK vs. A/NK diagram. Petrographical and geochemical data indicate that the porphyritic biotite granite which intruded at the last period originated from distinct parental magma from two-mica granite, garnet-bearing two-mica granite, and mica-granite. On the basis of Rb/Sr vs. Rb/Ba diagram and Al2O3/TiO2 vs. CaO/Na2O, it is inferred the porphyritic biotite granite originated from protolith with less pelitic composition than 3 other granitic rocks. The enriched values of lithophile elements of Cs, Rb, and Ba and negative trough of Nb, P, Ti on spider diagram suggest that the peraluminous Jurassic granitic rocks in Gwangdeoksan area formed in subduction tectonic environment. Whole-rock zircon saturation thermometer indicates that the granitic rocks in the study area were melted at 692-795℃.
Proceedings of the Mineralogical Society of Korea Conference
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2001.06a
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pp.95-95
/
2001
The Yeongdong basin is one of the pull-apart basins in the southwestern part of the Korean Peninsula that has developed during Cretaceous sinistal fault movement. The bimodal igneous activities (basalts and rhyolites) in the basin appear to be closely associated with the basin development. Here, we discuss the origin of the igneous rocks using chemical and radiogenic isotope data. Basaltic (48.4-52.7 wt% SiO$_2$) and rhyolitic (70.3-70.8 wt% SiO$_2$) rocks are slightly alkalic in a total alkali-silica diagram. The rhyolitic rocks with have unusually high K$_2$O contents (5.2-6.0 wt%). The basaltic rocks show an overall pattern of within-plate basalt in a MORB-normalized spider diagram, but have distinct negative anomaly of Nb, which indicates a significant amount of crustal component in the magma. The basaltic rocks plot within the calc-alkaline basalt field in the Hf/3-Th-Ta and Y/l5-La/10-Nb/8 discrimination diagrams. The eNd(T) values of the basaltic rocks (-13.6 to 14.3) are slightly higher than those of the rhyolitic rocks (-14.1 to 15.2), and the initial Sr isotopic ratios of the former (0.7085-0.7093) are much lower than those of the latter (0.7140-0.7149). However, the initial Nd and Sr isotope ratios of the igneous rocks in the Yeongdong basin are similar to those of the nearby Cretaceous igneous rocks in the Okcheon belt. The Pb isotope ratios plot within the field of Mesozoic granitoids outside of the Gyeongsang basin in Pb-Pb correlation diagrams. Since a basaltic magma requires the mantle source, the enriched isotopic signatures and negative Nb anomaly of the basaltic rocks suggest two possibilities for their origin: enriched mantle lithospheric source, or depleted mantle source with significant amount of crustal contamination. However, we prefer the first possibility since it would be difficult for a basaltic magma to maintain its bulk composition when it is significantly contaminated with granitic crustal material. The slightly more enriched isotopic signatures of rhyolitic rocks also suggest two possibilities: differentiate of the basaltlc magma with some crustal contamination, or direct partial melting of the lower crust. Much larger exposed volume of the rhyolitic rocks, compared with the basaltic rocks, indicates the latter possibility more favorable.
The development of insecticidal soaps made by various fatty acids and organic control for insect by using of insecticidal soap(fatty acid salts) were employed. And the results were as follows: 1. To determine the input volume of potassium hydroxide for saponification of fatty acids, there were compared to individual acid value of fatty acids. in case of coconut fatty acids for saponification was 266.3 mg of potassium hydroxide(266 g/1kg of fatty acid) was calculated. 2. To make the 25% soap content by coconut fatty acids, there was required for the 266g of potassium hydroxide and 3,459 liter of soft water. Then the liquor of soap was 4,644 liter. 3. The progress of insecticidal soap made by fatty acids was accomplished indirect heating and stirring reactor (1)to make the volume of potassium hydroxide solution and warming up(90$^{\circ}$) (2)input volume of individual fatty acid (3)more than 30 min stirring reaction (4)cooling progress. 4. Insecticidal value of insecticidal soap was observed more than 92% the dilution of 50 dilution solution in consecutive 5 days of 2 treatments of the 25% soap made by coconut fatty acids on the red pepper. And insecticidal value of insecticidal soap was observed more than 94% the 100 dilution solution in consecutive 5 days of 3 treatments of the 25% soap made by coconut fatty acids on red pepper and cabbage. 5. The treatment of two times of 25% soap made by coconut fatty acids at the 50 dilution solution and 100 dilution solution in spider mite on red bean has 100% insecticidal value. 6. There was no observation phytotoxic sypmtons on red pepper in field, except for 25% soap made by carprylic acid. 7. Over the two times over 0.1% in addition of isopropyl alcohol was to improvement the insecticidal effect, but there was no effect in addition of diatomaceous earth.
Economic injury levels (EILs) and economic control threshold (ET) were estimated for the Tea red spider mite, Tetranychus kanzawai Kishida(Acari, Tetranychidae) in Rubus coreanus Miquel. T. kanzawai density increased until the early-July and thereafter decreased in all plots except the non-innoculation plot where initial density of the mite were different each 0, 5, 10, 20 and 40 adults per plant branch on May 7 in 2008. And the occurrence of the densities were increased higher innoculated density than different innoculation density. The yield was decreased with increasing initial mite density and thereby the rates of yield loss was increased with increasing initial mite density. And T. kanzawai occurrence density, yields and the rates of yield loss, where initial density of the mite were different each 0, 2, 5, 10 and 20 adults per plant branch on May 8 in 2009 were similar tendency to 2008 year results. The relationship between initial T. kanzawai densities and the yield losses was well described by a linear regression, Y = 0.6545X + 3.0425 ($R^2$ = 0.93) in 2008, Y = 0.9031X + 2.0899($R^2$ = 0.96) in 2009. Based on the relationship, the number of adults per plant branch(EILs) which can cause 5% loss of yield was estimated to be approximately 3.0 in 2008 and 3.2 in 2009. And the ET was estimated to be approximately 2.4 in 2008 and 2.6 in 2009. The relationship between initial T. kanzawai densities and occurrence density of mid-May considering the best spray timing against T. kanzawai was well described by a linear regression, Y = 0.471X + 2.495($R^2$ = 0.95) in 2008, Y = 0.9938X + 3.1858($R^2$ = 0.96) in 2009. Based on the relationship, the number of adults per leaf(ET) in mid-May which can cause 5% loss of yield was estimated to be approximately 3.6 in 2008 and 5.8 in 2009.
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