Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Acaricidal Efficacy of Herbal Extracts against Tetranychus urticae (Acarina: Tetranychidae)

점박이응애, Tetranychus urticae (Acarina: Tetranychidae)에 대한 약용식물 추출물의 살비 효과

  • Lee, Jung-Su (Plant Protection Institute) ;
  • Ham, Eun-hae (Osangkinsect) ;
  • Choo, Ho-Yul (Dept. of Applied Biology, Gyeongsang National Univ.(Insti. of Agri. and Life Sci.)) ;
  • Lee, Suk-Jun (Department of Ecological Science, Kyungpook National Univ.) ;
  • Lee, Dong-Woon (Department of Ecological Science, Kyungpook National Univ.)
  • 이정수 (식물보호연구소) ;
  • 함은혜 (오상킨섹트) ;
  • 추호렬 (경상대학교 응용생물학과(농업생명과학연구원)) ;
  • 이석준 (경북대학교 생태과학과) ;
  • 이동운 (경북대학교 생태과학과)
  • Received : 2011.10.28
  • Accepted : 2011.12.20
  • Published : 2011.12.30
⟨ Previous Next ⟩

Abstract

Methanol and hexane extracts from 35 species in 27 families of herbal plants were evaluated for their acaricidal activities against two-spotted spider mite, Tetranychus urticae by leaf-dipping and spraying methods in laboratory, pot and field, respectively. Acaricidal activities were different depending on herbal plants. When T. urticae was released on leaves of Phaseolus vulgaris var. humilis that were dipped in the 1,000 ppm hexane extracts from fruits of Torreya nucifer and Daphan genkwa, seeds of Xanthium strumarium and Pharbitis nil at one minute they experienced 56.8, 47.8, 47.7, and 47.7% mortalities, respectively. Dipping time influenced acaricidal activity, that is, one minute dipping was more effective than 30 second dipping of Phaseolus vulgaris var. humilis leaves. However, extracts of X. strumarium and P. nil seeds had comparatively high activities. Concentration of hexane extract of X. strumarium and P. nil seeds revealing LC50 were 1,824 and 1,899 ppm, respectively. Extracts of both plants from hot and cold water were not effective representing <20% mortality at 1,000 ppm. However, hexane extracts of X. strumarium and P. nil seeds were effective against T. urticae on P. vulgaris var. humilis representing 76.3 and 71.3% mortalities in pot, respectively. Control effects of hexane extract of P. nil seed were 50.8 and 35.1% at 2,000 and 1,000 ppm against T. urticae on Chrysanthemum morifolium in greenhouse, respectively.

점박이응애(Tetranychus urticae)에 대한 살비활성을 가지는 물질을 알아보기 위하여 약용식물 25과 35종의 메탄올 또는 헥산 추출물을 이용하여 잎 침지법과 살포법으로 실내와 pot, 야외에서 실험을 수행하였다. 살비활성은 약용식물 종류별로 차이를 보였다. 강남콩(Phaseolus vulgaris var. humilis) 잎을 1,000 ppm 농도의 추출물에 1분간 침지한 후 점박이응애에 대한 치사율을 조사한 결과, 비자나무(Torreya nucifera) 열매 추출물과 팥꽃나무(Daphne genkwa)와 도꼬마리(Xanthium strumarium), 나팔꽃(Pharbitis nil) 씨앗 추출물이 각각 56.8%와 47.8, 47.7, 47.7%의 살비활성을 나타내었다. 잎 침지시간을 30초로 단축시켜 점박이응애에 대한 살비효과검정 시는 1분 침지에 비하여 효과가 감소하였고, 나팔꽃과 도꼬마리의 살비 활성이 가장 높았다. 도꼬마리와 나팔꽃 씨앗 헥산 추출물의 반수치사농도는 각각 1,824 ppm과 1,899 ppm이었다. 두 식물의 1,000 ppm 온수 및 냉수 추출물은 점박이응애에 대한 살비효과가 20% 이하로 낮았다. Pot의 강낭콩에 점박이응애를 접종한 후 분무법으로 추출물을 1,000 ppm 농도로 살포한 결과 도꼬마리와 나팔꽃 씨앗 헥산추출물은 각각 76.3과 71.3%의 높은 살비활성을 나타내었다. 그리고 온실에서 국화의 점박이응애를 대상으로 나팔꽃 씨앗 헥산 추출물을 2,000 ppm과 1,000 ppm으로 처리 시에는 50.8%와 35.1%의 방제가를 보였다.

Keywords

Acknowledgement

Supported by : 농림수산식품부

References

  1. Ahn, Y. J., J. K. Yoo, J. R. Cho, J. O. Moon, and S. C. Lee. 1996. Evaluation of effectiveness of AC303630 and flucycloxuron mixture against Tetranychus urticae (Acari: Tetranychidae) under laboratory and field conditions. Appl. Entomol. Zool. 31: 67-73.
  2. Ahn, K. S., S. Y. Lee, K. Y. Lee, Y. S. Lee, and G. H. Kim. 2004. Selective toxicity of pesticides to the predatory mite, Phytoseiulus persimilis and control effects of the two-spotted spider mite, Tetranychus urticae by predatory mite and pesticide mixture on rose. Korean J. Appl. Entomol. 43: 71-79.
  3. Bae, K. H. 2000. The medicinal plants of Korea. 654 pp. Kyo-Hak Publishing Co., Ltd. Seoul.
  4. Chiasson, H., A. Bélanger, N. Bostanian, C. Vencent, and A. Poliquin. 2001. Acaricidal properties of Artemisia absinthium and Tanacetum vulgare (Asteraceae) essential oils obtained by three methods of extraction. J. Econ. Entomol. 94: 167-171. https://doi.org/10.1603/0022-0493-94.1.167
  5. Cho, J. R., Y. H. Choi, N. J. Park, and K. Y. Cho. 1993. Comparative toxicities of selected acaricides against the twospotted spider mite to establish the screening system for new acaricidal chemical compounds. Korean J. Appl. Entomol. 32: 123-128.
  6. Cho, J. R., Y. J. Kim, Y. J. Ahn, J. K. Yoo, and J. O. Lee. 1995. Monitoring of acaricide resistance in field-collected populations of Tetranychus urticae (Acari: Tetranychidae) in Korea. Korean J. Appl. Entomol. 34: 40-45.
  7. Choi, W. I., S. G. Lee, H. M. Park, and Y. J. Ahn. 2004. Toxicity of plant essential oils to Tetranychus urticae (Acari: Tetranychidae) and Phytoseiulus persimilis (Acari: Phytoseiidae). J. Econ. Entomol. 97: 553-558. https://doi.org/10.1603/0022-0493-97.2.553
  8. Croft, B. A., S. C. Hoyt, and P. H. Westigard. 1987. Spider mite on fruits, revised: organotin and acaricide resistance management. J. Econ. Entomol. 80: 304-311.
  9. Hall, F. R. and D. C. Ferree. 1975. Influence of twospotted spider mite populations on photosynthesis of apple leaves. J. Econ. Entomol. 68: 517-520.
  10. Hiremath, I. G., Y. J. Ahn, S. I. Kim, B. R. Choi, and J. R. Cho. 1995. Insecticidal and acaricidal activities of african plant extracts against the brown planthopper and two-spotted spider mite. Korean J. Appl. Entomol. 34: 200-205.
  11. Ho, C. C. 2000. Spider mite problems and control in Taiwan. Exp. Appl. Acarol. 24: 453-462. https://doi.org/10.1023/A:1006443619632
  12. Huang, K. C. 1993. The pharmacology of Chinese herbs. p. 160. CRC Press, Boca Raton.
  13. Jung, D. Y., H. Ha, H. Y. Lee, C. Kim, J. H. Lee, K. H. Bae, J. S. Kim, and S. S. Kang. 2008. Triterpenoid saponins from the seeds of Pharbitis nil. Chem. Pharm. Bull. 56: 203-206. https://doi.org/10.1248/cpb.56.203
  14. Isman, M. B. 2000. Plant essential oils for pest and disease management. Crop Protection 19: 603-608. https://doi.org/10.1016/S0261-2194(00)00079-X
  15. Kim, D. I. 1994. Ecological characteristics of Tetranychus kanzawai and Amblyseius longispinosis in integrated pest management of tea. Ph. D. dissertation, Chonnam National University.
  16. Kim, D. I., C. H. Paik, J. D. Park, S. S. Kim, and S. G. Kim. 2000. Relative toxicity of neemAzal-T/S to the predacious mite, Amblyseius womersleyi (Acari: Phytoseiidae) and the twospotted spider mite, Tetranychus urticae (Acari: Tetranychidae). Korean J. Appl. Entomol. 39: 53-58.
  17. Kim, D. I., J. D. Park, S. G. Kim, H. Kuk, M. S. Jang, and S. S. Kim. 2005a. Screening of some crude plant extracts for their acaricidal and insecticidal efficacies. J. Asia-Pacific Entomol. 8: 93-100. https://doi.org/10.1016/S1226-8615(08)60076-X
  18. Kim, D. K., C. K. Shim, D. W. Bae, Y. S. Kawk, M. S. Yang and H. K. Kim. 2002. Identification and biological characteristics of an antifungal compound extracted from cocklebur (Xanthium strumarium) against Phytophthora drechsleri. Plant Pathol. J. 18: 288-292. https://doi.org/10.5423/PPJ.2002.18.5.288
  19. Kim, E. H., H. K. Kim, and Y. J. Ahn. 2003. Acaricidal activity of clove bud oil compounds against Dermatophagoides farinae and Dermatophagoides pteronyssinus (Acari: Pyroglyphidae). J. Agric. Food Chem. 51: 885-889. https://doi.org/10.1021/jf0208278
  20. Kim, G. H., S. Chol, N. J. Park, and K. Y. Cho. 1994. Inheritance if resistance in dicofol-selected strain of the two-spotted spider mite, Tetranychus urticae Koch, and its cross resistance. Korean J. Appl. Entomol. 33: 230-236.
  21. Kim, I. T., Y. M. Park, J. H. Won, H. J. Jung, H. J. Park, J. W. Choi and K. T. Lee. 2005b. Methanol extract of Xanthium strumarium L. possesses anti-inflammatory and anti-nociceptive activities. Biol. Pharm. Bull. 28: 94-100. https://doi.org/10.1248/bpb.28.94
  22. Kim, J., S. K. Lee, J. M. Kim, Y. R. Kwon, T. H. Kim, and J. S. Kim. 2008. Effect of temperature on development and life table parameters of Tetranychus urticae Koch (Acarina: Tetranychidae) reared on eggplants. Korean J. Appl. Entomol. 47: 163-168. https://doi.org/10.5656/KSAE.2008.47.2.163
  23. Kim, J. B. 2005. Pathogen, insect and weed control effects of secondary metabolites from plant. J. Korean Soc. Appl. Biol. Chem. 48: 1-15.
  24. Kim, K. H., S. K. Ha, S. U. Choi, S. Y. Kim and K. R. Lee. 2011. Bioactive phenolic constituents from the seeds of Pharbitis nil. Chem. Pharm. Bull. 59: 1425-1429. https://doi.org/10.1248/cpb.59.1425
  25. Kim, K. H., S. U. Choi, M. W. Son and K. R. Lee. 2010. Two new phenolic amides from the seeds of Pharbitis nil. Chem. Pharm. Bull. 58: 1532-1535. https://doi.org/10.1248/cpb.58.1532
  26. Kim, S. S. and S. C. Lee. 1989. Development of arcricide resistance and cross-resistance in Tetranychus urticae (Acarina: Tetranychidae). Korean J. Appl. Entomol. 28: 146-153.
  27. Kim, Y. J., H. E. Lee, S. W. Lee, G. H. Kim, and Y. J. Kim. 1999. Toxicity of tebufenpyrad to Tetranychus urticae and Amblyseius womersleyi under laboratory and field conditions. J. Econ. Entomol. 92: 187-192.
  28. Kwon, J. H., Y. J. Ahn, H. W. Kwon, K. S. Jang, and K. Y. Cho. 1994. Larvicidal and antifeeding activities of oriental medicinal plant extracts against Plutella xylostella (Lepidoptera: Yponomeutoidae) and Spodoptera litura (Lepidoptera: Noctuidae). Korean J. Appl. Entomol. 33: 225-229.
  29. Kyung, S. H. and Y. H. Yoon, 2000. Repellent activity of methanol extracts of native plants against Aedes albopictus and Blateria gemanica. Korean J. Pestic. Sci. 4: 18-20.
  30. Lee, I. H., M. S. Seol, and J. D. Park. 2005. Repellent and pesticidal effect of Ginkgo biloba leaves extracts on the Tetranichus urticae, Aphi gossypii and Myzus persicae. J. Korean Soc. Appl. Biol. Cem. 48: 150-154.
  31. Lee, J. S., H. Y. Choo, and D. W. Lee. 2011. Nematicidal efficacy of herbal extracts against Meloidogyne hapla. Korean J. Appl. Entomol. 50%: In press.
  32. Lee, S. G., J. D. Park, C. Song, K. Y. Cho, S. G. Lee, M. K. Kim, and H. S. Lee. 2001. Insecticidal activities of various vegetable extracts against five agricultural insect pests and four stored-product insect pests. Korean J. Pestic. Sci. 5: 18-25.
  33. Lee, S. Y., J. S. Yoo, S. J. Moon, S. G. Lee, C. S. Kim, S. C. Shin, and G. H. Kim. 2003a. Fumigant and repellency effects of terpenes against the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae). Korean J. Appl. Entomol. 42: 249-255.
  34. Lee, S. Y., K. S. Ahn, C. S. Kim, S. C. Shin, and G. H. Kim. 2004. Inheritance and stability of stability of etoxazole resistance in twospotted spider mite, Tetranychus urticae, and its cross resistance. Korean J. Appl. Entomol. 43: 43-48.
  35. Lee, S. W. 1990. Studies on the pest status and integrated mite management in apple orchards. Ph. D. dissertation, Seoul National University.
  36. Lee, Y. S., M. H. Song, K. S. Ahn, K. Y. Lee, J. W. Kim, and G. H. Kim. 2003b. Monitoring of acaricide resistance in twospotted spider mite populations from rose greenhouses in Korea. J. Asia-Pacific Entomol. 5: 237-240.
  37. Ma, Y. T., M. C. Huang, F. L. Hsu and H. F. Chang. 1998. Thiazinedione from Xanthium strumarium. Phytochemistry 48: 1083-1085. https://doi.org/10.1016/S0031-9422(98)00084-3
  38. Mahmoud, A. A. 1998. Xanthanolides and xanthane epoxide derivatives from Xanthium strumarium. Planta Medica. 64: 724-727. https://doi.org/10.1055/s-2006-957566
  39. McNab, S. C. and P. H. Jerier. 1993. Flowering, fruit set, and yield response of 'Bartlett' pear to leaf-scorch damage by twospotted spider mite. J. Econ. Entomol. 86: 486-493.
  40. Mitchell, R. 1973. Growth and population dynamics of a spider mite (Tetranychus urticae K., Acarina: Tetranychidae). Ecology 54: 1349-1355. https://doi.org/10.2307/1934198
  41. Momen, F. M., S. A. A. Amer, and A. M. Refaat. 2001. Influence of mint and peppermint on Tetranychus urticae and some predacious mites of the family Phytoseiidae (Acri: Tetranychidae: Phytoseiidae). Acta Phytopathologica et Entomologica Hungarica 36: 143-153. https://doi.org/10.1556/APhyt.36.2001.1-2.17
  42. Moon, H. C., J. R. Lim, J. Kim, J. Ryu, B. R. Ko, D. H. Kim, and C. Y. Hwang. 2006. Biological control of Tetranychus urticae by Phytoseiulus persimilis in eggplant greenhouse houses. Korean J. Appl. Entomol. 45: 173-177.
  43. Park, C. G., M. H. Lee, J. K. Yoo, J. O. Lee, and B. R. Choi. 1995. Relative toxicity of abamectin to the predatory mite Amblyseius womersleyi Schicha and two spotted spider mite Tetranychus urticae Koch. Korean J. Appl. Entomol. 34: 360-367.
  44. Park, J. H., K. Y. Ryu, B. M. Lee, and H. J. Jee. 2008. Effect of COY (cooking oil and yolk mixture) on control of Tetranychus urticae. Korean J. Appl. Entomol. 47: 249-254. https://doi.org/10.5656/KSAE.2008.47.3.249
  45. Park, I. K., J. D. Park, C. S. Kim, S. C. Shin, Y. J. Ahn, S. C. Park, and S. G. Lee. 2002. Insecticidal and acaricidal activities of domestic plant extracts against five major arthropod pests. Korean J. Pestic. Sci. 6: 272-278.
  46. Park, S. M., H. J. Jung, S. H. Han, S. H. Yeo, Y. W. Kim, H. G. Ahn, H. S. Kim and T. S. Yu. 2005. Antifungal activity of extract from Xanthium strumarium L. against plant pathogenous fungi. J. Life Science. 15: 692-695. https://doi.org/10.5352/JLS.2005.15.5.692
  47. Prakash, A. and J. Rao. 1997. Botanical pesticides in agriculture. 461 pp. CRC Press, Inc. Boca Raton.
  48. Saxena, V. K. and S. K. Mondal. 1994. A xanthanolide from Xanthium strumarium. Phytochemistry 35: 1080-1082. https://doi.org/10.1016/S0031-9422(00)90678-2
  49. SAS Institute. 1999. SAS OnlineDoc, version 8. SAS Institute, Cary, NC.
  50. Shi, G. L., L. L. Zhao, S. Q. Liu, H. Cao, S. R. Clarke, and J. H. Sun. 2006. Acaricidal activities of extracts of Kochia scoparia against Tetranychus urticae, Tetranychus cinnabarinus, and Tetranychus viennensis. J. Econ. Entomol. 99: 858-863. https://doi.org/10.1603/0022-0493-99.3.858
  51. Singh, N., P. Khatri, K. C. Samantha, and R. Damor. 2010. Antipyretic activity of methanolic extract of leaves of Quisqualis indica Linn. IJPRD. 2: 122-126.
  52. Song, C., G. H. Kim, S. J. Ahn, N. J. Park, and K. Y. Cho. 1995. Acaricide susceptibilities of field-collected populations of two-spotted spider mite, Tetranychus urticae from apple orchards. Korean J. Appl. Entomol. 34: 328-333.
  53. Stacey, D. L., I. J. Wyatt, and R. J. Chambers. 1985. The effect of glasshouse red spider mite damage on the yield of tomatoes. J. Hort. Sci. 60: 517-523.
  54. Takafuji, A., A. Ozawa, H. Nemoto, and T. Gotoh. 2000. Spider mites of Japan: their biology and control. Exp. Appl. Acarol. 24: 319-335. https://doi.org/10.1023/A:1006453209154
  55. Wilson, L. T., P. J. Trichilo, and D. Gonzalez. 1991. Spider mite infestation rate and initiation: effect on cotton yield. J. Econ. Entomol. 84: 593-600.
  56. Yang, Y. C., I. K. Park, E. H. Kim, H. S. Lee, and Y. J. Ahn. 2004. Larvicidal activity of medicinal plant extracts against Aedes aegypti, Ochlerotatus togoi, and Culex pipiens pallens (Diptera : Culicidae). J. Asia-Pacific Entomol. 7: 227-232. https://doi.org/10.1016/S1226-8615(08)60220-4
  57. Yu, J. S., D. K. Seo, E. H. Kim, J. B. Han, K. S. Ahn, and G. H. Kim. 2005a. Inheritance and cross resistance of bifenazate resistance in twospotted spider mite, Tetranychus urticae. Korean J. Appl. Entomol. 44: 151-156.
  58. Yu, J. S., J. S. Bae, D. K. Shin, and G. H. Kim. 2005b. Repellency of the constituents of caraway oil, Carum carvi against Tetranychus urticae. Korean J. Appl. Entomol. 44: 161-164.