• KSBB Journal
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• v.13 no.1
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• pp.83-89
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• 1998

Using a cellulose macroporous microcarrier, HeLa cells were cultivated in 100mL spinner flask(Bellco Co., USA) and confluent cell laden microcarriers were subcultured by bead-to-bead cell transfer method. In macroporous mcirocarrier-HeLa system viable suspended cells played an important role in bead-to-bead cell transfer and that could be increased by use of RPMI-1640, a calcium-ion-reduced-media and high speed agitation. Successful bead-to-bead cell transfers were performed continuously three time in spinner flask. We applied this technique to produce recombinant Vaccinia virus which express $\beta$-galactosidase. Recombinant protein yield of bead-to-bead transferred culture was comparable to conventional microcarrier cultures that were inoculated by cells detached from T-flask. Although trypsinization is a useful method for subculturing microcarriers in some cases, that process adds quality control problem and handling steps to large scale cell production. There fore, bead-to-bead cell transfer technique offers another convenient and efficient scale-up method for continuous microcarrier cultures.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.44 no.2
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• pp.79-85
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• 2018

Objectives: The aim of this study was to evaluate the effects of herbal extracts on bone regeneration. Two known samples were screened. Materials and Methods: We previously established a rat calvaria defect model using a combination of collagen scaffold and herbal extracts. An 8 mm diameter trephine bur with a low-speed dental hand piece was used to create a circular calvaria defect. The experimental group was divided into 4 classifications: control, collagen matrix, Danshen with collagen, and Ge Gan with collagen. Animals in each group were sacrificed at 4, 6, 8, and 10 weeks after surgery, and bone regeneration ability was evaluated by histological examination. Results: Results revealed that both Danshen and Ge Gan extracts increased bone formation activity when used with collagen matrix. All groups showed almost the same histological findings until 6 weeks. However, after 6 weeks, bone formation activity proceeded differently in each group. In the experimental groups, new bone formation activity was found continuously up to 10 weeks. In the Danshen and Ge Gan groups, grafted materials were still present until 10 weeks after treatment, as evidenced by foreign body reactions showing multinucleated giant cells in chronic inflammatory vascular connective tissue. Conclusion: Histological analyses showed that Danshen and Ge Gan extractions increased bone formation activity when used in conjunction with collagen matrix.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.85.1-85.1
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• 2017

We develop a coronagraph to measure the coronal electron density, temperature, and speed by observing the linearly polarized brightness of solar corona with 4 different wavelengths. Through the total solar eclipse on 21 August 2017, we test an operating software of a prototype coronagraph working with two sub-systems of two motorized filter wheels and a CCD camera that are controlled by a portable embedded computer. A Core Flight System (CFS) is a reusable software framework and set of reusable software applications which take advantage of a rich heritage of successful space mission of NASA. We use the CFS software framework to develop the operating software that can control the two sub-systems asynchronously in an observation scenario and communicate with a remote computer about commands, housekeeping data through Ethernet. The software works successfully and obtains about 160 images of 12 filter sets (4 bandpass filters and 3 polarization angles) during the total phase of the total solar eclipse. For the future, we can improve the software reliability by testing the software with a sufficient number of test cases using a testing framework COSMOS. The software will be integrated into the coronagraph for balloon-borne experiments in 2019.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.8
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• pp.645-651
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• 2015

The objective of this study is to experimentally investigate the effect of design parameter on the mass ratio of a central-driven ejector. The design parameters are the primary nozzle area and distance ratios, diffuser exit-area ratio and mixing-tube length ratio. The experimental setup was an open-loop continuous circulation system which has a movable nozzle ejector, an electric motor-pump, a water tank, a control panel and high-speed camera unit. We calculated the mass ratio using the measured primary and suction-flow rates with the experimental parameter of primary water-flow rate or pressure. The results showed that the mass ratio increased with the primary nozzle distance ratio and mixing tube length ratio, while the mass ratio decreased with the primary nozzle-area ratio and diffuser exit-area ratio.

• The Journal of the Korea Contents Association
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• v.12 no.12
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• pp.14-24
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• 2012

DVC (Distributed Video Coding) techniques need feedback channel for parity bit control to achieve the good RD performances, however, this causes the DVC system to have high decoding latency. In order to implement in real time environments and to accelerate commercializations, many research works have been focusing on the development of fast video decoding algorithm. As one of the real time implementations, this paper deals with a novel DVC scheme suitable for some application areas where source statistics such as motion information can be provided to the encoder side from the decoder side. That is, this paper propose a fast distributed video decoding scheme to improve the decoding speed by using the feedback of motion information derived in SI generation. Through computer simulations, it is shown that the proposed method outperforms the conventional fast DVC decoding schemes.

• Journal of the Korean Society for Precision Engineering
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• v.30 no.7
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• pp.762-768
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• 2013

This study investigated the effect of whole-body vibration on muscle function and muscular reaction in the knee joint. We recruited thirty healthy subjects and divided them into a training group, who experienced whole-body vibration, and a control group, who did not. The training group performed whole-body vibration exercises for 30 min per day, 3 days a week, for 8 weeks. We measured knee joint torque to estimate muscle strength and reaction, using BIODEX System 3. Knee joint peak torque and total work performed increased significantly in the training group, and muscle acceleration time decreased. These results suggest that stimulation by whole-body vibration can improve muscle strength and reaction by improving muscle tone and increasing blood temperature and flow speed in muscular fiber. Our results also indicate that 4 weeks of exercise with whole-body vibration is required to improve the reaction response, and six weeks to improve strength.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.39 no.1
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• pp.130-139
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• 2016

The purpose of this paper is to analyze the problems and the sources of defective products and draw improvement plans in a small plastic boat manufacturing process using TOC (Theory Of Constraints) and statistical analysis. TOC is a methodology to present a scheme for optimization of production process by finding the CCR (Capacity Constraints Resource) in the organization or the all production process through the concentration improvement activity. In this paper, we found and reformed constraints and bottlenecks in plastic boat manufacturing process in the target company for less defect ratio and production cost by applying DBR (Drum, Buffer, Rope) scheduling. And we set the threshold values for the critical process variables using statistical analysis. The result can be summarized as follows. First, CCRs in inventory control, material mix, and oven setting were found and solutions were suggested by applying DBR method. Second, the logical thinking process was utilized to find core conflict factors and draw solutions. Third, to specify the solution plan, experiment data were statistically analyzed. Data were collected from the daily journal addressing the details of 96 products such as temperature, humidity, duration and temperature of heating process, rotation speed, duration time of cooling, and the temperature of removal process. Basic statistics and logistic regression analysis were conducted with the defection as the dependent variable. Finally, critical values for major processes were proposed based on the analysis. This paper has a practical importance in contribution to the quality level of the target company through theoretical approach, TOC, and statistical analysis. However, limited number of data might depreciate the significance of the analysis and therefore it will be interesting further research direction to specify the significant manufacturing conditions across different products and processes.

• Journal of Advanced Research in Ocean Engineering
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• v.4 no.3
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• pp.115-123
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• 2018

This study was carried using the new approach method to design appropriately the Loading Arm length and the alarm setting according to ship movements on Loading and Unloading marine Berth. The quasi-static mooring analysis was performed to estimate 110,000DWT ship's movements based on environmental conditions such as wind, current and wave. The mooring motion of the ship is very important to determine the loading arm scope, and in this case, the operation condition is performed on the ship without considering the damaged condition of the mooring line because the ship movement in case of damage is larger than intact, and all operations are stopped, the loading arm being released due to control system. From the result of mooring analysis, motion displacements, velocities and accelerations were simulated. They were used to simulate the maximum drifting speeds and distances. The maximum drifting speeds were checked to be satisfied within drifting speed limits. The total maximum drifting distances were simulated with alarm steps of the new approach method. Finally, the loading arm envelopes using the total maximum drifting distances were completed. Therefore, it was confirmed that the new approach method for loading arm envelopes and alarm settings was appropriate from the above results. In the future, it will be necessary to perform the further advanced dynamic mooring analysis instead of the quasi-static mooring analysis and to use the precise computer program analysis for various environments and ship movement conditions.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.12
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• pp.117-124
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• 2009

In this paper, we have developed communication modules for ubiquitous transportation sensor network (u-TSN). The developed module can be used for intelligent transportation services. The developed systems are based on IEEE 802.11a and IEEE 802.11g technologies for vehicle and infrastructure systems, respectively. We have found that the throughput for the developed systems is at maximum around 15 Mbps. It is reduced to 10 Mbps at a long distance and high speed condition. The performance is enough to support traffic control services in dense traffic condition.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.6
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• pp.891-898
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• 2012

The study for the passenger's comfortableness of vehicles and the arousal of car drivers has been done widely. On the other hand, there are few studies for the locomotive engineers. Human error means that the mistakes made by human, recently it receives attention in the field of safety engineering and human engineering. Comparing the operating condition of train with car, because of the simplification of the visual stimulus, the arousal level on the train goes down easily. The arousal level down makes judgement down, the accident risk from human error is getting bigger. In this study, we measured bio-signals(ECG, EDA, PPG, respiration and EEG) from 6 locomotive engineers to evaluate their arousal state while they operated the train. Also we recorded the 3 axes acceleration signal showing the vibration state of train. Also, the existence of tunnels were simultaneously measured. At the station section where the train speed goes down, the size of vector's sum decreases because of reduced vibration. Beta component in EEG tends to increase at the entering point of each station and tunnel. It is due to the arousal reaction and tension growth. The mean SCR(skin conductance response) was more increased in neutral section. As the button control movement (body movement) increases in the neutral section, it is appeared that SCR increase. RR interval tends to gradually increase during train operation for 1 hour 40 minutes. However, It tends to sharply decrease at the stop station because strong concentration needed to stop train on the exact point. The engineer's arousal reaction can be checked through analysing the bio-signal change during train operation. Therefore, if this analysing result is adopted to the sleepiness prevention caution system, it will be useful for the safety train operation.