• Fisheries and Aquatic Sciences
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• v.12 no.3
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• pp.227-235
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• 2009

The marine toxic dinoflagellate Alexandrium catenella has been implicated in numerous paralytic shellfish poisoning (PSP) events in many countries. Due to difficulties in rapidly identifying A. catenella, field-based study of this species has been problematic. The present study developed a TaqMan format A. catenella-specific probe for real-time PCR assay (specific to Korean genotype) based on LSU rDNA sequence information for studying geographic and temporal distribution of the species in surface sediments and water columns of Jinhae Bay, Korea. The field survey from 2007 to 2008 revealed that A. catenella occurred in most seasons at low densities, mostly below 1 cell $mL^{-1}$, and was more abundant in spring (maximum cell density of 2 cells $mL^{-1}$) when shellfish exceed the quarantine toxin level for PSP toxins in Jinhae Bay.

• Mycobiology
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• v.46 no.3
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• pp.205-214
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• 2018

The order Mucorales, the largest in number of species within the Mucoromycotina, comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of undiscovered taxa in Korea, two novel mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were isolated from specific habitats including freshwater and fecal samples, respectively. On the basis of their morphological characteristics and sequence analyses of internal transcribed spacer and large subunit ribosomal DNA, the CNUFC-GWD3-9 and CNUFC-EGF1-4 isolates were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively. It is ecologically, pathologically, and mycologically significant to find such rare zygomycetous fungi in such specific habitats.

• International Journal of Oral Biology
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• v.36 no.2
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• pp.79-82
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• 2011

The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strainspecificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.

• Journal of Microbiology
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• v.40 no.2
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• pp.146-150
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• 2002

A multiplex polymerase chain reaction (PCR) assay was developed for the identification of two Candida species-albicans and dubliniensis. Three sets of primers were selected from different genomic sequences to specifically amplify a 516 bp fragment within the tops gene, specific for several species of the genus Candida (CCL primers); a 239 bp fragment within the $\alpha$INT1 gene, specific for Candida albicans (CAL primers); and a 175 bp fragment within the ALSD1 gene, specific for Candida dubliniensis (CDL primers). Using the primers in conjunction (multiplex PCR), we were able to detect both C. albicans and C. dubliniensis and to differentiate between them. The detection limit of the PCR assay was approximately 10 cells per milliliter of saline. Thus, this multiplex PCR assay can be applied for differentiation of C. albicans and C. dubliniensis from clinical specimens.

• Journal of Ecology and Environment
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• v.42 no.4
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• pp.278-284
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• 2018

Plant functional traits have been shown to be useful to understand how and why ecosystems and their components vary across environmental heterogeneity or gradients. This study investigated how plant functional (leaf) traits vary according to an elevation-associated environmental gradient. Environmental gradients (mean annual temperature and precipitation) were quantified, and leaf traits (leaf area, specific leaf area, leaf nitrogen, leaf phosphorus, leaf carbon, and leaf C/N ratio) of the understory woody plant species Acer pseudosieboldianum were examined across an elevational gradient ranging from 600 to 1200 m in a Baegunsan Mountain in Gwangyang-si, Jeollanam-do, South Korea. The results showed that mean annual temperature and precipitation decreased and increased along with elevation, respectively. Leaf area of the plant species decreased slightly with increasing elevation, while specific leaf area did not differ significantly. Leaf nutrients (nitrogen, phosphorus, and carbon concentrations) were higher at high elevations, but leaf C/N ratio decreased with elevation.

• Animal cells and systems
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• v.13 no.2
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• pp.187-203
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• 2009

Neustonic calanoid copepods were collected at the South Sea of Korea using a David-Hempel neuston net. Four Pantel/apsis species (P. armata Giesbrecht, P. rega/is Dana, P. vil/asa Brady and P. yamadae Mori) were identified and the former three species were first recorded in the Korea. We present redescription of the four species with SEM micrographs of female genitalia. Female genital structure is species-specific and can be very useful for species classification in the genus Pantel/apsis. Also we discuss zoogeography of Pantel/apsis species and provide with a key to the species hitherto known in the Korean waters.

• Journal of Korean Society of Forest Science
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• v.4 no.1
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• pp.39-41
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• 1965

(1) The durability of fifteen species of hard woods grown in Kangwon-do in relation to Polystictus Versecolor L. Fr. and Irpex consors Berk was studied. (2) The least affected hard wood species by Polystutus Versecolor L. Fr. were Q. serrata, C. crenata, M. amurensis, F. rhynchophylla and J. mandschrica; the moderatly affected species were Q. Variabilis, U. Davidiana var japonica, B. latiforia, C. controversa, P. padus and A. mono; and the most highly affected species were P. serrulata var. spondanea, S. obassia, T. amurensis and B. schmidtii. The least affected hard wood species by Irpex consors Berk, were U. Davidiana var japonica, C. crenata, M. amurensis, F. rhynchophylla, and J. mandshurica; the moderatly affected species were Q. Valiabilis, Q. serrata, P. serrulata var. spondanea, C. controversa, and A. mono; and the most highly affected species were B. latiforia, P. padus, S. obassia, T. amurensis, and J-mandshurica. (3) No difference was obscured on the durabilities on effected by kind of fungus. (4) Correlationship between specific gravity and durability of the woods was not significant.

• Parasites, Hosts and Diseases
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• v.25 no.2
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• pp.207-209
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• 1987

A systematic mosquito taxonomic study in Korea was established by Lee in 1971. Since then, many species have been recorded as new to Korea. However, much of this taxonomic information has been distributed in a number of scientific publications. This fork attempts to combine this information into a single document, as well as to synthesize revised taxonomic synonyms of species cccurring in Korea. Four species, Culex vora.x, Culex kangi, Aedes kobayashii and Culiseta kanayamensis listed in our previous paper, are synonyms of Culex kalifaxii, Cutex jackseni, Aedes alehtorowi and Culiseta bergrothi, respectively. Culex annulus is a misidentified species of Culex pseudovishnui. The subspecies Cutes pipiens quinquefassiatus and subgenus Manscnia (Ccquillettidia) were treated as full species and genus respectively in a catalog of the mosquitoes of the world. However, not all current authors agree to those specific and generic treatment. Based on available publications the total number of mosquito species recorded in Korea is now 52 representing 9 genera. Forty-five of these 52 are confirmed species with preserved specimen representation. As collection of specimen continues, there is a possibility that representative of 7 unconfirmed species will be found. 1 hope this paper will provide useful information for entomology workers who are interested in mosquito taxonomy in Korea.

• Journal of Species Research
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• v.10 no.1
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• pp.57-62
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• 2021

A new species, Miscanthus wangpicheonensis of section Kariyasua, is described and illustrated from the morphological characteristics based on a type specimen collected from Gyeongsangbuk-do. This new species is similar to Miscanthus sacchariflorus (Maxim.) Hack., and M. longiberbis (Hack.) Nakai. The characters which distinguish the above two species are short leaves and racemes, callus hairs 1-1.5-times longer than spikelet, and well-developed awns of spikelet. The specific epithet, "wangpicheonensis", is based on the name of the locality where the new species is found. We assume this new species originated from the hybridization of Miscanthus sacchariflorus (Maxim.) Hack., and M. longiberbis (Hack.) Nakai. Miscanthus wangpicheonensis is found in only one locality in Uljin-gun, Gyeongsangbuk-do, Republic of Korea.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1107-1112
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• 2012

Outbreaks of foodborne diseases associated with Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae frequently occur in countries having a dietary habit of raw seafood consumption. For rapid identification of different Vibrio species involved in foodborne diseases, whole-cell protein pattern analysis for 13 type strains of 12 Vibrio species was performed using SDS-PAGE analysis. Pathogenic Vibrio species such as V. parahaemolyticus, V. vulnificus, V. cholerae, V. alginolyticus, V. fluvialis, and V. mimicus were included in the 12 Vibrio species used in this study. Each of the 12 Vibrio species showed clearly specific band patterns of its own. Two different strains of V. parahaemolyticus showed two different SDS-PAGE whole-cell protein patterns, giving the possibility of categorizing isolated strains in the same V. parahaemolyticus species into two subgroups. The 36 Vibrio isolates collected from sushi restaurants in Busan were all identified as V. parahaemolyticus by comparing their protein patterns with those of Vibrio type strains. The identified isolates were categorized into two different subgroups of V. parahaemolyticus. The whole-cell protein pattern analysis by SDS-PAGE can be used as a specific, rapid, and simple identification method for Vibrio spp. involved in foodborne diseases at the subspecies level.