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Multiplex Polymerase Chain Reaction Assay for Simultaneous Detection of Candida albicans and Candida dublinensis  

Lim, Young-Hee (Department of Clinical Laboratory Science, College of Health Science, Korea University, Sungbuk-ku, Seoul)
Lee, Do-Hyun (Department of Clinical Pathology, Korea University Kuro Hospital, Seoul, South Korea)
Publication Information
Journal of Microbiology / v.40, no.2, 2002 , pp. 146-150 More about this Journal
Abstract
A multiplex polymerase chain reaction (PCR) assay was developed for the identification of two Candida species-albicans and dubliniensis. Three sets of primers were selected from different genomic sequences to specifically amplify a 516 bp fragment within the tops gene, specific for several species of the genus Candida (CCL primers); a 239 bp fragment within the $\alpha$INT1 gene, specific for Candida albicans (CAL primers); and a 175 bp fragment within the ALSD1 gene, specific for Candida dubliniensis (CDL primers). Using the primers in conjunction (multiplex PCR), we were able to detect both C. albicans and C. dubliniensis and to differentiate between them. The detection limit of the PCR assay was approximately 10 cells per milliliter of saline. Thus, this multiplex PCR assay can be applied for differentiation of C. albicans and C. dubliniensis from clinical specimens.
Keywords
Candide albicans; Candida dubliniensis; multiplex PCR;
Citations & Related Records

Times Cited By Web Of Science : 3  (Related Records In Web of Science)
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