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http://dx.doi.org/10.5657/fas.2009.12.3.227

Abundance of the Toxic Dinoflagellate Alexandrium catenella in Jinhae Bay, Korea as Measured by Specific Real-time PCR Probe  

Park, Tae-Gyu (Ecology and Oceanography Division, National Fisheries Research and Development Institute (NFRDI))
Kang, Yang-Soon (Ecology and Oceanography Division, National Fisheries Research and Development Institute (NFRDI))
Park, Young-Tae (Ecology and Oceanography Division, National Fisheries Research and Development Institute (NFRDI))
Publication Information
Fisheries and Aquatic Sciences / v.12, no.3, 2009 , pp. 227-235 More about this Journal
Abstract
The marine toxic dinoflagellate Alexandrium catenella has been implicated in numerous paralytic shellfish poisoning (PSP) events in many countries. Due to difficulties in rapidly identifying A. catenella, field-based study of this species has been problematic. The present study developed a TaqMan format A. catenella-specific probe for real-time PCR assay (specific to Korean genotype) based on LSU rDNA sequence information for studying geographic and temporal distribution of the species in surface sediments and water columns of Jinhae Bay, Korea. The field survey from 2007 to 2008 revealed that A. catenella occurred in most seasons at low densities, mostly below 1 cell $mL^{-1}$, and was more abundant in spring (maximum cell density of 2 cells $mL^{-1}$) when shellfish exceed the quarantine toxin level for PSP toxins in Jinhae Bay.
Keywords
Alexandrium catenella; Dinoflagellate; PSP toxins; Real-time PCR probe;
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