• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.1
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• pp.27-34
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• 2016

Garlic has drawn attention as a food material for its anti-oxidative and anti-inflammatory properties as well as for prevention and treatment of cancer. In order to increase efficiency, various aging methods for garlic have been attempted. In particular, thermally processed garlic is known to have higher biological activities due to its various chemical changes during heat treatment. Therefore, in this study, we investigated the anti-oxidative effects of garlic extracts aged at low temperature ($60{\sim}70^{\circ}C$). In the results, 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis (3-ethylbenzo-thiazoline-6-sulfonate) radical scavenging activities and ferric reducing ability of low temperature-aged garlic (LTAG) were similar to those of raw garlic. LTAG also showed decreased lipopolysaccharide (LPS)-induced production of reactive oxygen species, although there were not significant differences among samples. In addition, xanthine oxidase activity was inhibited by LTAG; the 15 days and $60^{\circ}C$ extract showed outstanding inhibition compared with the others. To understand the molecular mechanisms behind the anti-oxidative activity of LTAG, we performed quantitative real-time PCR analysis. The 30 days and $70^{\circ}C$ extract upregulated mRNA expression of antioxidant enzymes such as Cu/Zn-superoxide dismutase (SOD), Mn-SOD, glutathione peroxidase, and catalase in LPS-stimulated RAW 264.7 cells. This result indicates that LTAG can be a functional food as a nature antioxidant and antioxidant substance.

• Journal of Korean Society of Forest Science
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• v.80 no.4
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• pp.436-444
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• 1991

To increase both the production of mushrooms and the usages of the residues (used media) for roughage, the pinchips(PC) and sawdusts(SD) of three Korean oak species were tested as cultivating media for Lentinus edodes with varing the composition of the media. The results are as follows. 1. Organic acids(tannic acid and citric acid), which were added in the PC medium to enrich the quality of mushrooms, did not increase the growths of the mycelia and the quality. 2. The mushrooms were able to be harvested from six months after beginning the cultivation. No significant differences were found among the qualities of the mushrooms produced by various treatments. 3. The contents of crude proteins(1.82-4.55%) in the used media were higher than those in woods (c.a. 0.7%), and the total digestible nutrients (44.0-46.0%) in the used medium were not much different from those in rice straws (c.a.48.0%). 4. The contents of some essential amino acids (methionine in the used medium of the tannic acid added PC or S.D. and isoleucine, phenylalanine and lysine in the used medium of the untreated PC or SD) were higher than those in the control (raw media). The contents of a few other essential amino acids (threonine, valine and arginine) were lower in the used media. Most nonessential amino acids in the used media, particularly in the used bed-log, appeared to decrease than those in the control.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.849-854
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• 2010

This study was conducted to investigate the variation of seed and germination characteristics among populations and among individuals within populations of Abies koreana Wilson distributed in Korea. Cones was collected from 4 natural populations and their seven seed qualities as well as three germination behaviors was measured and tested. Statistical analysis showed that there were significant differences among populations as well as among individuals within populations in 8 traits except for percentage of sound seeds and seed vigor. Coefficients of variation in percentage of sound seeds, germination percentage and germination rate is relatively high (43.8~57.2%) compared to other traits (11.0~28.3%). The germination of seed started on the seventeen day after sowing and it was completed within seventy-nine days. The final seed germination capacity was found high except for Mt. Duckyu (8.5%) and varied from 39.7% to 47.7%. Mean germination time ranged between 38.1 and 43.6 days and germination rate varied from 1.98 to 1.39 ea./days. As a result of simple correlation analysis between seed characteristics and germination characteristics, the percentage of sound seeds showed a significant positive correlation with the germination percentage and the germination rate but showed a negative correlation with the mean germination time.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.81-86
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• 2008

This study describes conditions for plant regeneration from protoplasts-derived from embryogenic callus of satsuma mandarin. Plants were generated via somatic embryogenesis. Protoplasts isolated directly from nucellar callus induced from immature ovule of satsuma mandarin cv. Okitsu (Citrus unshiu Marc.) were cultured in 0.6M $BH_3$ medium. Cell division and plating efficiency were affected by protoplast culture method. The liquid over solid method was the most effective for formation of microcalli. Most of microcalli grew rapidly and transferred onto embryoid formation medium. Optimum embryoid formation medium was MT medium containing 1.5 g/L malt extract, 0.146 M sucrose and the medium for plantlet regeneration was MS medium containing 0.09M sucrose, 1.0 mg/L $GA_3$. No differences were noticed in growth habits and leaf characters such as shape, thickness, and colour between protoplast-derived plants and nucellar seedlings. This plant regeneration system from protoplasts-derived from embryogenic callus provides an alternative way for producing new scion and rootstock cultivar from citrus species which can not be crossed.

• Journal of Life Science
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• v.20 no.10
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• pp.1538-1543
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• 2010

This study was conducted to examine the genetic diversity and population structure of 17 Brassica juncea populations in Korea. The technique of random amplified polymorphic DNA (RAPD) produced 60 polymorphic loci and 18 monomorphic loci. In a simple measure of intraspecies variability by the percentage of polymorphic bands, the Jindo population of Cheonnam showed the highest (29.5%). The cultivar exhibited the lowest variation (12.8%). Mean number of alleles per locus (A) and the effective number of alleles per locus ($A_E$) were 1.221 and 1.167, respectively. As the typical populations of this species were small, isolated, and patchily distributed in their natural populations, they maintained a low level of genetic diversity of fourteen primers. On a per locus basis, total genetic diversity values ($H_T$) and interlocus variation in the within-population genetic diversity ($H_S$) were 0.347 and 0.141, respectively. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) was 0.589. This indicated that about 58.9% of the total variation was among populations. The estimate of gene flow, based on $G_{ST}$, was very low among Korean populations of B. juncea ($N_m$=0.617). These results suggest that the geological distance dispersal of wild B. juncea is the best event. RAPD markers are very effective in classifying natural population levels of B. juncea in Korea.

• Journal of Life Science
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• v.13 no.5
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• pp.732-739
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• 2003

The purpose of this study was to determine if the addition of spermatozoa into the culture medium could influence the nuclear maturation of denuded porcine germinal vesicle (GV) oocytes in vitro. Cumulus-oocyte complexes were collected from follicles of 3 to 5 mm in diameter, The cumulus and corona cells were removed from oocytes. Porcine denuded oocytes were cultured in tissue culture medium containing spermatozoa. After 48 h culture, oocytes were examined for the evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II (M II). The proportion of oocytes reaching M II stage was significantly (P<0.01) increased in the oocytes cultured in media containing spermatozoa compared to those in media without spermatozoa $(31.9\pm1.8%\; vs\; 14.9\pm1.0%)$.No differences in the rates of M II were observed among the different period of spermatozoa exposure nor among the spermatozoa from different species. The proportion of oocytes reaching M II stage was significantly different between high and low concentrations of spermatozoa. The present study suggests that mammalian spermatozoa contain a substance(s) that improves nuclear maturation in vitro of GV oocytes. Enhancing effect of spermatozoa for oocytes maturation in vitro is a highly dose-dependent.

• Journal of the Korean Chemical Society
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• v.17 no.3
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• pp.174-181
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• 1973

Dichlorodiacetatotitanium(IV) dissolves in alcohols with chemical reaction. Such solvolytic reaction of $TiCl_2(OAc)_2$with various alcohols has been studied by means of solution NMR spectroscopy and chemical analysis of the isolated products. The reaction of $TiCl_2(OAc)_2$ with primary alcohols has shown to be a quantitative two-step ligand substitution process as shown in the following: $TiCl_2(OAc)_2+ROH{\to}TiCl_2(OAc)_2(OR)+AcOH$ $TiCl_2(OAc)_2(OR)+ROH{\to}TiCl_2(OAc)_2+AcOH$The molecular form initially soluble in organic solvents has been found to be the monosubstituted species $TiCl_2(OAc)(OR)$. Alcoholysis with t-butyl alcohol has shown remarkable differences. When the mole ratio of t-butyl alcohol to $TiCl_2(OAc)_2$ is less than 1/2, the following reaction is dominant. $TiCl_2(OAc)_2+t-ButOH{\to}TiCl_2(OAc)_2+t-ButCl$However, at higher mole ratio another substitution process resembling the first step reaction with primary alcohols is competitively accompanied. The reaction with t-butyl alcohol also differs from that with primary alcohols in that only one either of the two chloro-or acetato-ligands in $TiCl_2(OAc)_2$ is subjected to substitution.

• Journal of agricultural medicine and community health
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• v.4 no.1
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• pp.62-70
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• 1979

In order to evaluate the present status of hook-worm infection in the rural areas in Korea, stool examinations were undertaken in several selected rural villages in each year of 1970 and 1977. The methods employed were cellophane thick smear technique and brine floatation technique in 3,454 specimens collected from 7 different rural villages for the prevalence rate of hookworm infection and Stoll's dilution egg counting technique for the intensity of hookworm infection. The incidences of Ancylostoma duodenale and Necator americanus infections were determind by polyethylene tube coproculture and by the evacuated adult worms after treatment with anthelmintics to the patients who were infected by hookworms. The results are as follows : 1) In 1970, 323(18.6%) out of 1,737 specimens collected from 4 different rural areas were positive for hook worm infection and the ranges of prevalence rates in those suveyed areas were shown as 6.9 to 38.2 per cent. On the other hand, 144(8.2%) out of 1,717 specimens collected from 3 different rural areas in 1977 were shown as positive for hookworm infection and 7.5 to 10.6 per cent of prvalence rates were shown in the surveyed areas. 2) In the age group under 10 years, there was no infection of hookworm in the studied areas in 1977, however in 1970 the prevalence of the hookworm infection was shown as very low in the age group of 5~9. On the whole, the hookworm prevalence steadily increased from the age of 15 in the studied areas in 1970, however in 1977 the prevalence increased from the age of 20 to the older age groups. 3) There was not observed much differences in the intensity of hookworm infection among the studied areas in 1970 and 1977. The mean EPG in each studied areas were shown under 1,000 EPG and mean numbers of evacuated worms per infected persons were counted as 9.4 to 15. 1. 4) Both A.duodenale and N.americanus have been found in all studied areas but the incidences and st-ate of distribution of two species of hookworn were shown as different in each localities.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.9 no.4
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• pp.164-172
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• 2004

Water temperature, salinity, the phytoplankton community and population of a marine photosynthetic ciliate, Mesodinium rubrum (=Myrionecta rubra), were monitored every 0.5-2 weeks in Gomso Bay and the Geum River Estuary from September 1999 to December 2000. Patterns of temporal variation of the M. rubrum population and phytoplankton community were compared with each other in relation to the differences in temporal fluctuation patterns of the water temperature and salinity in the two study areas. Higher population densities and more frequent blooms of M. rub rum in the Geum River Estuary than those in Gomso Bay could be due to the relatively higher nutrient input by freshwater influx in the Geum River Estuary. In the Geum River Estuary which experience more abrupt and irregular fluctuations of salinity, M. rubrum with its greater tolerance to salinity change exhibited increased dominance while neritic diatoms such as Skeletonema costatum, Asterionellopsis glacialis, A. kariana, Chaetoceros debilis, Eucampia zodiacus, Paralia sulcata, Thalassiosira pacifica, T. nordenskioeldii showed decreased dominance compared with those in Gomso Bay. Thus, it is possible that M. rubrum replaces the dominant diatom species in coastal waters where artificial modification of coast lines as in the case of Saemankeum Reclamation Project should increase the frequency as well as the absolute scale of freshwater discharges.

• Parasites, Hosts and Diseases
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• v.35 no.4
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• pp.239-244
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• 1997

Genus Metasonimus has been a subject of taxonomic debates for several years. In morphological aspects, M. vokogawci has been thought to have three subtypes, namely Yokogawa type (M. yokogawai in strict sense) , Miyata type and Koga type. But dif- ferences in the intestinal pathology induced by these subtypes have not been studied yet. In this study we compared the pathological reactions inducted by M. yokoBnwai and Metcgonimus Miyata type using proliferating cell nuclear antigen (PCNA) index. Metacercariae (Mc) of M. yokogcuani were collected by artificial digestion of Plecoslossw oLtiveLis and Mc of Metngonimus Miyata type were collected from Zacco pEntvpus. Three hundreds Mc of each species were infected orally to ICR mice. The mice were sacrificed at 3, 6. 10. 16 and 23 days after infection and the small intestines were resected into three portions (proximal. middle, and distal) . Immunohistochemical staining for PCNA was done using PC-10 (DAKO-PCNA, CA, USA) The PCNA indices in M. Wokosnwci infected group on the 6th and 23rd day after infection were lower than in the control and Miyata type infect ed groups (p < 0.05) from all of the three intestinal regions. On the other hand, the control group and Ifetngonimus Miyata type infected group did not make any differences in PCNA indices. The villus/crypt WIC) ratio was also decreased significantly in M. Wokogcwni infected mice but not in Metagonimus Miyata type infected ones. It is suggested that M. wokogawci induce villous atrophy through a decrease in the cell proliferation at the crypt. The results of this study suggested that M. WokoBnwai induce more serious intestinal pathology than Metcsonimuf Miyata type.