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http://dx.doi.org/10.5010/JPB.2008.35.1.081

Plant regeneration from protoplasts-derived from embryogenic callus of Citrus  

An, Hyun-Joo (Citrus Experiment Station, NISA, RDA)
Lee, Dong-Hoon (Citrus Experiment Station, NISA, RDA)
Lee, Ji-Hyun (Citrus Experiment Station, NISA, RDA)
Choi, Young-Hun (Citrus Experiment Station, NISA, RDA)
Kang, Byoung-Cheorl (Dept. of Plant Science, CALS, Seoul National University)
Park, Hyo-Guen (Pepper & Breeding Institute, Seoul National University)
Publication Information
Journal of Plant Biotechnology / v.35, no.1, 2008 , pp. 81-86 More about this Journal
Abstract
This study describes conditions for plant regeneration from protoplasts-derived from embryogenic callus of satsuma mandarin. Plants were generated via somatic embryogenesis. Protoplasts isolated directly from nucellar callus induced from immature ovule of satsuma mandarin cv. Okitsu (Citrus unshiu Marc.) were cultured in 0.6M $BH_3$ medium. Cell division and plating efficiency were affected by protoplast culture method. The liquid over solid method was the most effective for formation of microcalli. Most of microcalli grew rapidly and transferred onto embryoid formation medium. Optimum embryoid formation medium was MT medium containing 1.5 g/L malt extract, 0.146 M sucrose and the medium for plantlet regeneration was MS medium containing 0.09M sucrose, 1.0 mg/L $GA_3$. No differences were noticed in growth habits and leaf characters such as shape, thickness, and colour between protoplast-derived plants and nucellar seedlings. This plant regeneration system from protoplasts-derived from embryogenic callus provides an alternative way for producing new scion and rootstock cultivar from citrus species which can not be crossed.
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