• Journal of Food Hygiene and Safety
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• v.16 no.4
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• pp.251-257
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• 2001

Listeria monocytogenes and L. ivanovii are important food-pathogens for human and animal. The diagnostic of Listeria in food using culture medium requires time and laborwork, because there are many other non-pathogenic species like L. innocua, L welshimeri, L. seeligeri and L. grayi in Genus Listeria. For these reasons, Lismix multiplex PCR method was developed as a rapid method for the detection and identification of Listeria. In this study we developed a practical system of Lis-mix PCR detection for the application to food samples and new developed Siw-mix III PCR system overall 69 listerial strains were successful species-identified and confirmed. Also, the Siw-mix III PCR system allows the species-specific identification among L. ivanovii, L. welshimeri and L seeligeri in a single PCR.

• Food Science and Biotechnology
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• v.16 no.1
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• pp.99-103
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• 2007

The purpose of this study was to develop a simple detection method, possibly at the species-level, that allows for large-scale screening of bifidobacteria. Human fecal samples were plated on MRS-raffinose agar containing cysteine and neomycin sulfate, serving as selective pressure for bifidobacteria, and 0.003%(w/v) bromocresol green. All of the test strains grew well on this medium at $37{\pm}1^{\circ}C$, forming white colonies surrounded by yellow halos, which presented a sharp contrast against the green background. In this disc assay, the required incubation time to develop a yellowish zone varied with the species of Bifidobacterium that was tested, allowing for differential counts and easy identification at the species-level: 10-14 hr for B. bifidum, 20-22 hr for B. catenulatum and B. infantis. and 24-25 hr for B. longum and B. breve. No apparent color was observed for B. angulatum and B. adolescentis 28 hr after inoculation. To evaluate the results of pH indicator-based identification, individual isolates were subjected to a colony-PCR experiment with genus-specific primers. The amplified products from the isolates were in good accordance with those from the reference strains at a level of 95% agreement. These results suggest that the present method could be conveniently applied to cell counts, as well as to the preliminary identification of bifidobacteria from a variety of sample types including human feces, dairy products, and commercial probiotic supplements.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.1-14
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• 2022

Moths have a well-developed sex pheromone communication system. Male moths exhibit an extremely sensitive and selective sex pheromone detection system so that they can detect the sex pheromone produced by conspecific females and locate them for successful mating. Using the pheromone detection system, male moths display characteristic stereotypic behavioral responses, flying upwind to follow intermittent filamentous pheromone strands in pheromone plume. The chemical composition of female sex pheromone in moths, typically comprised of multiple compounds, is species-specific. Male moths contain specialized pheromone receptor neurons on the antennae to detect conspecific sex pheromone accurately, and distinguish it from the pheromones produced by other species. The signals from pheromone receptor neurons are integrated and induce relevant behavior from the male moths. Male moths also contain olfactory sensory neurons in pheromone sensilla, specialized for pheromone-related behavioral antagonist compounds, which can enhance discrimination between conspecific and heterospecific pheromones. Here we review reports on the sex pheromone detection system in male moths and their related responses, and suggest future research direction.

• Mycobiology
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• v.32 no.3
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• pp.123-127
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• 2004

Sweet persimmons have been increasingly cultivated in the southern part of Korea. However, anthracnose disease caused by Colletotrichum species is one of the major hindrances in cultivation and productions. In this study, we used polymerase chain reaction(PCR) to detect Colletotrichum species with the AFLP(amplified fragment length polymorphism) method. In AFLP, we used E3(5'-GACTGCGTACCAATTCTA-3') and M1(5'-GATGAGTCCTGAGTAACAG-3') primer combination and, as a result, 262 bp segment was observed in Colletotrichum species only. Specific PCR primers were designed from the sequence data and used to detect the presence of the fungus in genomic DNA isolated from symptomless sweet persimmon plants. Based on sequence data for specific segments, Co.B1(5'-GAGAGAGTAGAATTGCGCTG-3') and Co.B2(5'-CTACCATTCTTCTA GGTGGG-3') were designed to detect Colletotrichum species. The 220 bp segment was observed in Colletotrichum species only, but not in other fungal and bacterial isolates.

• Mycobiology
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• v.37 no.4
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• pp.317-319
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• 2009

In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'- GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.

• Journal of Microbiology and Biotechnology
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• v.20 no.9
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• pp.1266-1275
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• 2010

Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderma species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared with the other isolates of Trichoderma species.

• The Korean Journal of Ecology
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• v.10 no.1
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• pp.17-22
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• 1987

This survey was carried out in four areas in southern Korea from May 1983 through April 1986. A total of 1, 717 house rats was caught by trapping technique throughout the 4 areas. The total of 1, 535 trombiculid mites (4 genera, 7 species) was found only among Rattus norvegicus. Six species were observed in Seoul and Yangsuri areas, and 5 species in Seongnam area. No trombiculid mites were found in the rats caught in Inchon area. The highest infection rate of trombiculid mites (25%) was recorded in the rats caught in Seongnam area. And Leptotrombidium palpale was abundant species in general (detection rate; 72.8%) In seasonal variation, the hightes number of trombiculid population was observed from October through December in all the areas surveyed, and especially Euschongastia koreaensis apperaed more in autumn and winter than in any other season. The values of specie diversity and evenness of trombiculid mites found in Yangsuri were comparatively higher than those from the other areas.

• Korean Journal of Animal Reproduction
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• v.12 no.1
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• pp.48-50
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• 1988

Flehmen is well-known response which often occurs during the process of courtship in most mammals. Recent studies with domestic ruminants suggest that the flehmen may be involved in the mechanism of transferring some pheromonal substances to vomeronasal organ. Thus, variety of its significancehas been supposed, besides that male animals may use it for estrus detection. In this experiment, 8 male, 3 female and3 castrated goats of Saanen and its hybrid were used to ascertain whether urine from alien species can induce flehmen as that from same species. Urine was collected from twenty species consisting of 15 mammals, 3 birds and 2 reptiles and frozen until use. Mostly urine was sprayed to the nose of goats, but some coagulated ones were sniffed. Duration of flehmen was scored to four ranks as 0, 1-19, 20-39 and >40 sec. Each urine sample induced the response in any goats. However, much difference in the in tensity was found between the samples and according to the reproductive state of the receptor goats. Although individual difference was manifest, male goats generally showed more intense response than did female. Castrated goats showed the intermediate pattern. Administration of antiandrogen to the male goat tended to reduce the response. The results indicate that flehmen in the goat could occur for the urine of alien species as that of same species and the androgen may be one of the factors regulating the response.

• Natural Product Sciences
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• v.19 no.1
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• pp.15-19
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• 2013

Analysis of flavonoid contents in the fruits of Acanthopanax species (A. chiisanensis, A. divaricatus, A. koreanum, A. senticosus, and A. sessiliflorus) was conducted by high performance liquid chromatography. A Discovery$^{(R)}$ C18 ($4.6{\times}250$ mm, 5 ${\mu}m$) column was used with a gradient mobile phase of water and acetonitrile (90 : 10 to 60 : 40 for 60 min) and UV detection was conducted at 350 nm. The contents of rutin, hyperin, quercetin, afzelin, and kaempferol were 0.063~0.540, 0.494~7.480, 0.584~0.704, 0.388~0.567, 0.190~0.471 mg/g, respectively, in the fruits of Acanthopanax species. Total content of flavonoids in the fruits of Acanthopanax species was highest in those of A. chiisanensis. Furthermore, hyperin was the most abundant compound in the fruits of Acanthopanax species. Consequently, our results demonstrate that the fruits of Acanthopanax species containing flavonoids have promising potential as a new income source of agriculture and industry in medicinal natural products, health supplements, and beverages.

• Food Science and Preservation
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• v.16 no.6
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• pp.965-970
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• 2009

PCR technology has been widely used to detect and quantify microbial pathogens in foodstuffs, because the technique is rapid, sensitive, and selective. In this study, detection of contaminating pathogenic bacteria on shells of chicken eggs was performed using both a commercial multiplex polymerase chain reaction (PCR) kit and a viable count method employing a selective medium. The PCR kit was capable of detecting Campylobacter jejuni, Escherichia coli O157:H7, Staphylococcus aureus, Bacillus cereus, Vibrio parahaemolyticus, Listeria monocytogenes, Yersinia enterocolitica, Salmonella species, and Shigella species. Using the PCR method, five bacterial species were detected from 30 samples (33.3%) of 90 batches of eggs commercially available in a market. PCR products from B. cereus, S. aureus, L. monocytogenes, Y. enterocolitica, and E. coli O157:H7 were detected, and the numbers and frequencies of positive samples were 17 (18.8%), 12 (13.3%), 15 (16.6%), 16 (17.7%),and 4 (4.4%), respectively. None of any Salmonella species, C. jejuni, V. parahaemolyticus, or Shigella species was detected in this study. The results of PCR testing were confirmed using a typical viable count method employing a selective medium. We suggest that the multiplex polymerase chain reaction (mPCR) assay is a rapid and reliable method for detection of pathogenic bacteria contaminating eggs.