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http://dx.doi.org/10.4489/MYCO.2009.37.4.317

Development of Specific Primer for Tricholoma matsutake  

Kim, Jang-Han (Department of Life Science, Graduate School, Dongguk University)
Han, Yeong-Hwan (Department of Life Science, Dongguk University)
Publication Information
Mycobiology / v.37, no.4, 2009 , pp. 317-319 More about this Journal
Abstract
In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'- GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.
Keywords
ITS; Tricholoma matsutake; Tricholomataceae;
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