• International Journal of Industrial Entomology and Biomaterials
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• 제25권1호
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• pp.75-79
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• 2012

Low-melting-temperature agarose gel solution, as a novel porogen was combined with a silk fibroin solution to generate interconnected porous networks. The porosity of the resultant silk fibroin-agarose scaffolds was greater than that of the scaffolds generated with agarose and deionized water. The porosities of silk fibroin scaffolds containing agarose gel at 0.5%, 1.0%, 1.5%, 2.0% [w/v] were 110.9%, 111.7%, 120.9%, and 123.0%, respectively. Lastly, the internal space generated in scaffolds after dissolution of the agarose gel provides a good environment for cell growth and movement within the scaffold.

• Biodesign
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• 제5권3호
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• pp.122-125
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• 2017

Receptor for advanced glycation end products (RAGE) is one of the single transmembrane domain containing receptors and causes various inflammatory diseases including diabetes and atherosclerosis. RAGE extracellular domain has three consecutive IgG-like domains (V-C₁-C₂ domain) which interact with various soluble ligands including heparan sulfate or HMGB1. Studies have shown that each ligand induces different oligomeric forms of RAGE which results in a ligand-specific signal transduction. The structure of mouse RAGE bound to heparan sulfate has been previously determined but the electron density map of heparan sulfate was too ambiguous that the exact position of heparin sulfate could not be defined. Furthermore, the complex structure of human RAGE and heparin sulfate still remains elusive. Therefore, to determine the structure, human RAGE was overexpressed using bacterial expression system and crystallized using the sitting drop method in the condition of 0.1 M sodium acetate trihydrate pH 4.6, 8 % (w/v) polyethylene glycol 4,000 at 290 K. The crystal diffracted to 3.6 Å resolution and the space group is C121 with unit cell parameters a= 206.04 Å, b= 68.64 Å, c= 98.73 Å, α= 90.00°, β= 90.62°, γ= 90.00°.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제22권3호
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• pp.356-359
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• 2000

Superior orbital fissure syndrome is characterized by ophthalmoplegia, ptosis of the eye, reflex dilation of the pupil, and anesthesia of the upper eyelid and forehead. This syndrome may be the result of craniofacial fractures as well as neoplasms of the retrobulbar space, hematomas in the orbital muscle cone and retrobulbar space, and hematoma and infection of the cavernous sinus. A case of superior orbital fissure syndrome is described.

• 환경생물
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• 제31권4호
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• pp.333-339
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• 2013

임상의 동일 공간에 생육하고 있는 제비꽃속 세 종(고깔제비꽃, 왜제비꽃, 남산제비꽃)의 영양생장적 특징과 광량의 관계를 통해 각 종의 전략자적 특징을 알아보았다. 본 연구는 광주광역시에 위치한 무등산의 다섯개 지점으로부터 1994년, 1995년, 2000년에 중점 조사가 수행되었고, 2007년과 2010년 추가 조사가 진행되었다. 각 종의 집단 내 개체수의 변동, 개체의 건중량과 엽수, 조도에 따른 종의 분포 유형 분석을 통해 세 종이 임상의 광양에 의해 서로 다른 생태적 지위를 얻고 있음을 알 수 있었다. 고깔제비꽃은 K-전략자로서, 왜제비꽃은 r-전략자로서, 남산제비꽃은 두 종의 중간에서 그 생태적 지위를 차지하는 것으로 해석되었다.

• 한국실내디자인학회논문집
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• 제28호
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• pp.44-50
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• 2001

This study propose the new concept of residence by analyzing the change of residence through the characteristic of popularity and degree of development of technology on home network information appliance for intelligent home. Accordingly, Cyber village represented as home automation and extend to information of society. it encourage need of information and multimedia of home. It expect home information infrastructure for accepting informations, which make smart home to linked home-working home-learning home-treatment. home-shopping and home-banking. The system of intelligent home is the intelligence of human-biology in the side of environmental friendly and multi-function. it distinguish the system of security, controlling system of inside environment, supporting system of house-working, automatic controlling, house working. Future house require to meet demand of young generation, such as small residental space, the multi-functional space, the flexible space, making mood for dual income couple and of single as intelligent home. Accordingly, basic purpose which are pleasantness, the safe and the convenience the mobile multi-function as well as networking with controlling of temperature, security, health-test, home-entertainment, home-office and consider environment together.

• BMB Reports
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• 제49권8호
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• pp.443-448
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• 2016

The arginylation branch of the N-end rule pathway is a ubiquitin-mediated proteolytic system in which post-translational conjugation of Arg by ATE1-encoded Arg-tRNA-protein transferase to N-terminal Asp, Glu, or oxidized Cys residues generates essential degradation signals. Here, we characterized the ATE1^−/− mice and identified the essential role of N-terminal arginylation in neural tube development. ATE1-null mice showed severe intracerebral hemorrhages and cystic space near the neural tubes. Expression of ATE1 was prominent in the developing brain and spinal cord, and this pattern overlapped with the migration path of neural stem cells. The ATE1^−/− brain showed defective G-protein signaling. Finally, we observed reduced mitosis in ATE1^−/− neuroepithelium and a significantly higher nitric oxide concentration in the ATE1^−/− brain. Our results strongly suggest that the crucial role of ATE1 in neural tube development is directly related to proper turn-over of the RGS4 protein, which participate in the oxygen-sensing mechanism in the cells.

• BMB Reports
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• 제47권12호
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• pp.666-672
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• 2014

The Wnt signaling pathway is well known to play major roles in skeletal development and homeostasis. In certain aspects, fracture repair mimics the process of bone embryonic development. Thus, the importance of Wnt signaling in fracture healing has become more apparent in recent years. Here, we summarize recent research progress in the area, which may be conducive to the development of Wnt-based therapeutic strategies for bone repair.

• Journal of Microbiology and Biotechnology
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• 제32권8호
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• pp.967-975
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• 2022

Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (MAGs) of the Komagataeibacter, which is a predominant genus in KMC, to understand their roles in the KMC after exposure to Mars-like conditions (outside the International Space Station) based on functional genetic elements. We constructed three MAGs: K. hansenii, K. rhaeticus, and K. oboediens. Our results showed that (i) K. oboediens MAG functionally more complex than K. hansenii, (ii) K. hansenii is a keystone in KMCs with specific functional features to tolerate extreme stress, and (iii) genes related to the PPDK, betaine biosynthesis, polyamines biosynthesis, sulfate-sulfur assimilation pathway as well as type II toxin-antitoxin (TA) system, quorum sensing (QS) system, and cellulose production could play important roles in the resilience of KMC after exposure to Mars-like stress. Our findings show the potential mechanisms through which Komagataeibacter tolerates the extraterrestrial stress and will help to understand minimal microbial composition of KMC for space travelers.

• Biodesign
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• 제7권1호
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• pp.24-27
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• 2019

Pnc1 converts nicotinamide to nicotinic acid to generate NAD⁺ through the Preiss-Handler pathway that is one of the NAD⁺-salvage pathway. By reducing levels of nicotinamide, an inhibitor of the NAD⁺-dependent histone deacetylase Sir2, yeast Pnc1 contributes gene silencing. In this study, to understand the structural features and molecular mechanism of nicotinamidase Pnc1, we overexpressed, purified, and crystallized the N-terminally His₆-tagged Pnc1 protein from Kluyveromyces lactis and obtained X-ray diffraction data at a resolution of 2.2 Å. The crystals of the K. lactis Pnc1 (KlPnc1) belonged to space group P2₁2₁2₁ with unit cell parameters a=38.5, b=77.3, c=83.3, and α=β=γ= 90°. There is one molecule in the asymmetric unit.

• 한국산림과학회지
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• 제90권5호
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• pp.593-599
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• 2001

다변량분석법(주성분분석법, PCA)을 이용하여 무척추동물 군집에 백강균 처리가 미치는 영향을 검정하였다. 좌표공간내의 군집들간의 거리를 이용하여, 군집구조에 미치는 요인들의 영향을 통계 검정하였다. 백강균 처리는 낙엽과 토양의 무척추동물 군집에 유의한 영향을 주지 않았다.