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http://dx.doi.org/10.7852/ijie.2012.25.1.075

Development of a Porous Scaffold-Manufacturing Method by Blending Silk Fibroin and Agarose Polymer Solutions  

Park, Seung-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association)
Kweon, Hae-Yong (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association)
Goo, Tae-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association)
Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association)
Jo, You-Young (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association)
Choi, Gwang-Ho (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.25, no.1, 2012 , pp. 75-79 More about this Journal
Abstract
Low-melting-temperature agarose gel solution, as a novel porogen was combined with a silk fibroin solution to generate interconnected porous networks. The porosity of the resultant silk fibroin-agarose scaffolds was greater than that of the scaffolds generated with agarose and deionized water. The porosities of silk fibroin scaffolds containing agarose gel at 0.5%, 1.0%, 1.5%, 2.0% [w/v] were 110.9%, 111.7%, 120.9%, and 123.0%, respectively. Lastly, the internal space generated in scaffolds after dissolution of the agarose gel provides a good environment for cell growth and movement within the scaffold.
Keywords
Silk fibroin; Agarose hydrogel solution; Scaffold; Tissue engineering;
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1 Sofia S, McCarthy MB, Gronowicz G, Kaplan DL (2001) Functionalized silk-based biomaterials for bone formation. J. Biomed. Mater Res 54, 139-148.   DOI   ScienceOn
2 Soliman S, Sant S, Nichol JW, Khabiry M, Traversa E, Khademhosseini A (2011) Controlling the porosity of fibrous scaffolds by modulating the fiber diameter and packing density. J. Biomed. Mater Res. Part A 96A, 566?574.   DOI   ScienceOn
3 Virgilio N, Desjardins P, Pepin MF, L'Esperance G, Favis BD (2005) High contrast imaging of interphases in ternary polymer blends using focused ion beam preparation and atomic force microscopy. Macromolecules 38, 2368.   DOI   ScienceOn
4 Virgilio N, Desjardins P, L'Esperance G, Favis BD (2009) In situ measure of interfacial tensions in ternary and quaternary immiscible polymer blends demonstrating partial wetting. Macromolecules 42, 7518e29.   DOI   ScienceOn
5 Virgilio N, Marc-Aurele C, Favis BD (2009) Novel self-assembling close-packed droplet array at the interface in ternary polymer blends. Macromolecules 42, 3405e16.   DOI   ScienceOn
6 Virgilio N, Sarazin P, Favis BD (2010) Towards ultraporous poly(L-lactide) scaffolds from quaternary immiscible polymer blends. Biomaterials 31, 5719-5728.   DOI   ScienceOn
7 Horiuchi S, Matchariyakul N, Yase K, Kitano T (1997) Morphology development through an interfacial reaction in ternary immiscible polymer blends. Macromolecules 30, 3664e70.   DOI   ScienceOn
8 Kim UJ, Park J, Kim HJ, Wada M, Kaplan DL (2005) Threedimensional aqueous-derived biomaterial scaffolds from silk fibroin. Biomaterials 26, 2775-2785.   DOI   ScienceOn
9 Makaya K, Terada S, Ohgo K, Asakura T (2009) Comparative study of silk fibroin porous scaffolds derived from salt/water and sucrose/hexafluoroisopropanol in cartilage formation. J Biosci Bioeng 108, 68-75.   DOI   ScienceOn
10 Mathew P, Nitya G, Selvamurugan N, Nair SV, Furuike T, Tamura H, Jayakumur R (2010) Preparation and characterization of chitosan-gelatin/nanohydroxyapatite composite scaffolds for tissue engineering applications. Carbohydrate Polymers 80, 687-694.   DOI   ScienceOn
11 Roy X, Sarazin P, Favis BD (2006) Ultraporous nanosheath materials by layer-bylayer deposition onto co-continuous polymer-blend templates. Adv Mat 18, 1015e9.
12 Sarazin P, Favis BD (2003) Morphology control in co-continuous poly(L-lactide)/polystyrene blends: a route towards highly structured and interconnected porosity in poly(L-lactide) materials. Biomacromolecules 4, 1669-1679.   DOI   ScienceOn
13 Sarazin P, Roy X, Favis BD (2004) Controlled preparation and properties of porous poly(L-lactide) obtained from a co-continuous blend of two biodegradable polymers. Biomaterials 25, 5965-5978.   DOI   ScienceOn