• 제목/요약/키워드: SolE

검색결과 385건 처리시간 0.034초

Novel mechanism of a CDH1 splicing mutation in a Korean patient with signet ring cell carcinoma

  • Kim, Sol-Lip;Ki, Chang-Seok;Kim, Kyoung-Mee;Lee, Myoung-Gun;Kim, Se-Hwa;Bae, Jae-Moon;Kim, Jong-Won
    • BMB Reports
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    • 제44권11호
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    • pp.725-729
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    • 2011
  • We report a novel mechanism of a CDH1 splicing mutation in a patient with signet ring cell carcinoma of the stomach. A 27-year-old man complaining of aggravated dyspepsia was diagnosed with signet ring cell carcinoma. Both his father and uncle had died of stomach cancer at a young age. DNA sequencing analysis of the CDH1 gene revealed a splice site mutation (c.833-2A>G). By RNA/cDNA sequencing analysis, CDH1 c.833-2A>G generated a new acceptor site within intron 6, causing the insertion of a 79-bp intronic sequence between exon 6 and 7 (r.833-79_833-1ins), and resulting in a frame shift. E-cadherin immunohistochemical staining revealed a loss of CDH1 expression. This study reveals the disease-causing mechanism of this splicing mutation, and emphasizes the need for functional studies using RNA samples for the accurate interpretation of detected splicing variant. This is the first reported case of a CDH1 mutation in a Korean patient.

학습공간으로 이용되는 대학주변 커피전문점의 조명 실태 및 평가 (The Actual State and Evaluation of Artificial Lighting on Coffee Houses Using Study Place around University)

  • 최솔지;최윤정
    • KIEAE Journal
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    • 제11권6호
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    • pp.53-62
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    • 2011
  • This study aimed at suggesting improvement of lighting environment of the coffee house using study place. To this end, a series of field investigation was conducted in four possible target coffee houses around university. The field measurement included measurement of general illuminance and tabletop illuminance, observing illumination condition, and status of artificial lighting. Also, on-site questionnaire survey was administrated to 80 users of field measurement targets about using characteristics of coffee house and user's subjective response on light environment. The results are summarized as follows: (1) According to questionnaire survey, most of users checked 'learning (study and reading)' in 'purpose of coffee house using', and 'slightly dark' was checked most in each subjective response (brightness on general space and on tabletop at daytime/night); (2) as results of measurements on general illuminance and on tabletop illuminance during daytime, only one coffee house was suitable for standard; (3) as results of measurements on illuminance during night, all target coffee houses were not met the standard; (4) as results of uniformity ratios, almost uniformities of general illuminance were not met the standard except one case. The common problems of lighting environment of coffee house were analyzed as lack of daylight illumination e.g. having low amount of sunshine from skylight, un-uniformity of insolation by floor plan and absence of window blind, and un-uniformity of artificial luminous intensity e.g. lack of the number or brightness of artificial lighting, using the indirect lighting, using only local lighting, and non-uniform arrangement of artificial lighting.

Phenothiazine계 전도성고분자의 합성 및 유기박막태양전지로의 적용 연구 (Synthesis and Photovoltaic Properties of Conducting Polymers Based on Phenothiazine)

  • 유한솔;박용성
    • 공업화학
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    • 제24권1호
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    • pp.93-98
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    • 2013
  • 본 연구에서는 고온 고압반응을 통하여 4종의 전도성 고분자 poly[(N-butyl-phenothiazine)-sulfide] (PBPS), poly[(N-hexyl-phenothiazine)-sulfide] (PHPS), poly[(N-decyl-phenothiazine)-sulfide] (PDPS), poly[(N-(2-ethylhexyl)-phenothiazine)-sulfide] (PEHPS)를 합성하였다. 각 단계의 합성된 화합물의 구조는 $^1H-NMR$을 통하여 확인하였고, UV-Vis, cyclic voltammetry, GPC를 이용하여 합성된 고분자의 물성을 확인하였다. PBPS, PHPS, PDPS, PEHPS의 최대흡수파장은 각각 338, 341, 340, 334 nm이었으며, 각 고분자의 광학적 밴드 갭은 3.11, 3.13, 3.16, 3.05 eV이었다. 유기박막태양전지로서의 적용가능성을 확인하기 위해 합성된 고분자를 전자 받개 물질인 $PC_{71}BM$과 블렌딩하여 ITO/PEDOT : PSS/polymer (PBPS, PDPS) : $PC_{71}BM$ (1 : 3, w/w)/$BaF_2$/Ba/Al 구조의 소자를 제작하였고, solar simulator로 광전변환효율을 측정하였다. PBPS의 광전변환효율은 0.076%이었고, PDPS의 광전변환효율은 0.136%이었다.

Optimization of a microarray for fission yeast

  • Kim, Dong-Uk;Lee, Minho;Han, Sangjo;Nam, Miyoung;Lee, Sol;Lee, Jaewoong;Woo, Jihye;Kim, Dongsup;Hoe, Kwang-Lae
    • Genomics & Informatics
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    • 제17권3호
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    • pp.28.1-28.9
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    • 2019
  • Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000 each for up-and down-tags) different kinds of sense-strand probes on an array. In this study, we optimized the hybridization processes of an array for fission yeast. Compared to the first version of the array (11 ㎛, 100K) consisting of three sectors with probe pairs (perfect match and mismatch), the second version (11 ㎛, 48K) could represent ~10,000 up-/ down-tags in quadruplicate along with 1,508 negative controls in quadruplicate and a single set of 1,000 unique negative controls at random dispersed positions without mismatch pairs. For PCR, the optimal annealing temperature (maximizing yield and minimizing extra bands) was 58℃ for both tags. Intriguingly, up-tags required 3× higher amounts of blocking oligonucleotides than down-tags. A 1:1 mix ratio between up- and down-tags was satisfactory. A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. A tag intensity analysis showed that tag(s) of 4,316 of the 4,526 strains tested were represented at least once; 3,706 strains were represented by both tags, 4,072 strains by up-tags only, and 3,950 strains by down-tags only. The results indicate that this microarray will be a powerful analytical platform for elucidating currently unknown gene functions.

Synthesis and Characterization of CZTS film deposited by Chemical Bath Deposition method

  • Arepalli, Vinaya Kumar;Kumar, Challa Kiran;Park, Nam-Kyu;Nang, Lam Van;Kim, Eui-Tae
    • 한국재료학회:학술대회논문집
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    • 한국재료학회 2012년도 춘계학술발표대회
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    • pp.99.1-99.1
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    • 2012
  • The thin-film photovoltaic absorbers (CdTe and $Cu(In,Ga)Se_2$) can achieve solar conversion efficiencies of up to 20% and are now commercially available, but the presence of toxic (Cd,Se) and expensive elemental components (In, Te) is a real issue as the demand for photovoltaics rapidly increases. To overcome these limitations, there has been substantial interest in developing viable alternative materials, such as $Cu_2ZnSnS_4$ (CZTS) is an emerging solar absorber that is structurally similar to CIGS, but contains only earth abundant, non-toxic elements and has a near optimal direct band gap energy of 1.4 - 1.6 eV and a large absorption coefficient of ~104 $cm^{-1}$. The CZTS absorber layers are grown and investigated by various fabrication methods, such as thermal evaporation, e-beam evaporation with a post sulfurization, sputtering, non-vacuum sol-gel, pulsed laser, spray-pyrolysis method and electrodeposition technique. In the present work, we report an alternative aqueous chemical approach based on chemical bath deposition (CBD) method for large area deposition of CZTS thin films. Samples produced by our method were analyzed by scanning electron microscopy, X-ray diffraction, transmission electron microscopy, absorbance and photoluminescence. The results show that this inexpensive and relatively benign process produces thin films of CZTS exhibiting uniform composition, kesterite crystal structure, and some factors like triethanolamine, ammonia, temperature which strongly affect on the morphology of CZTS film.

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Evaluating the Headspace Volatolome, Primary Metabolites, and Aroma Characteristics of Koji Fermented with Bacillus amyloliquefaciens and Aspergillus oryzae

  • Seo, Han Sol;Lee, Sunmin;Singh, Digar;Park, Min Kyung;Kim, Young-Suk;Shin, Hye Won;Cho, Sun A;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • 제28권8호
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    • pp.1260-1269
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    • 2018
  • Production of good Koji primarily depends upon the selection of substrate materials and fermentative microflora, which together influence the characteristic flavor and aroma. Herein, we performed comparative metabolomic analyses of volatile organic compounds (VOCs) and primary metabolites for Koji samples fermented individually with Bacillus amyloliquefaciens and Aspergillus oryzae. The VOCs and primary metabolites were analyzed using headspace solid phase microextraction (HS-SPME) followed by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). In particular, alcohols, ketones, and furans were mainly detected in Bacillus-fermented Koji (Bacillus Koji, BK), potentially due to the increased levels of lipid oxidation. A cheesy and rancid flavor was characteristic of Bacillus Koji, which is attributable to high content of typical 'off-flavor' compounds. Furthermore, the umami taste engendered by 2-methoxyphenol, (E,E)-2,4-decadienal, and glutamic acid was primarily detected in Bacillus Koji. Alternatively, malty flavor compounds (2-methylpropanal, 2-methylbutanal, 3-methylbutanal) and sweet flavor compounds (monosaccharides and maltol) were relatively abundant in Aspergillus-fermented Koji (Aspergillus Koji, AK). Hence, we argue that the VOC profile of Koji is largely determined by the rational choice of inocula, which modifies the primary metabolomes in Koji substrates, potentially shaping its volatolome as well as the aroma characteristics.

InGaZnO 박막 트랜지스터의 전기 및 광학적 특성에 대한 전자빔 조사의 영향 (Influence of Electron Beam Irradiation on the Electrical and Optical Properties of InGaZnO Thin Film Transistor)

  • 조인환;박해웅;김찬중;전병혁
    • 한국재료학회지
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    • 제27권6호
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    • pp.345-349
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    • 2017
  • The effects of electron beam(EB) irradiation on the electrical and optical properties of InGaZnO(IGZO) thin films fabricated using a sol-gel process were investigated. As the EB dose increased, the electrical characteristic of the IGZO TFTs changed from semiconductor to conductor, and the threshold voltage values shifted to the negative direction. X-ray photoelectron spectroscopy analysis of the O 1s core level showed that the relative area of oxygen vacancies increased from 14.68 to 19.08 % as the EB dose increased from 0 to $1.5{\times}10^{16}electrons/cm^2$. In addition, spectroscopic ellipsometer analysis showed that the optical band gap varied from 3.39 to 3.46 eV with increasing EB dose. From the result of band alignment, it was confirmed that the Fermi level($E_F$) of the sample irradiated with $1.5{\times}10^{16}electrons/cm^2$ was located at the closest position to the conduction band minimum(CBM) due to the increase of electron carrier concentration.

Injectable hydrogels delivering therapeutic agents for disease treatment and tissue engineering

  • Lee, Jin Hyun
    • 생체재료학회지
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    • 제22권4호
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    • pp.235-248
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    • 2018
  • Background: Injectable hydrogels have been extensively researched for the use as scaffolds or as carriers of therapeutic agents such as drugs, cells, proteins, and bioactive molecules in the treatment of diseases and cancers and the repair and regeneration of tissues. It is because they have the injectability with minimal invasiveness and usability for irregularly shaped sites, in addition to typical advantages of conventional hydrogels such as biocompatibility, permeability to oxygen and nutrient, properties similar to the characteristics of the native extracellular matrix, and porous structure allowing therapeutic agents to be loaded. Main body: In this article, recent studies of injectable hydrogel systems applicable for therapeutic agent delivery, disease/cancer therapy, and tissue engineering have reviewed in terms of the various factors physically and chemically contributing to sol-gel transition via which gels have been formed. The various factors are as follows: several different non-covalent interactions resulting in physical crosslinking (the electrostatic interactions (e.g., the ionic and hydrogen bonds), hydrophobic interactions, ${\pi}$-interactions, and van der Waals forces), in-situ chemical reactions inducing chemical crosslinking (the Diels Alder click reactions, Michael reactions, Schiff base reactions, or enzyme-or photo-mediated reactions), and external stimuli (temperatures, pHs, lights, electric/magnetic fields, ultrasounds, or biomolecular species (e.g., enzyme)). Finally, their applications with accompanying therapeutic agents and notable properties used were reviewed as well. Conclusion: Injectable hydrogels, of which network morphology and properties could be tuned, have shown to control the load and release of therapeutic agents, consequently producing significant therapeutic efficacy. Accordingly, they are believed to be successful and promising biomaterials as scaffolds and carriers of therapeutic agents for disease and cancer therapy and tissue engineering.

철강산업부산물을 사용한 방조제용 그라우트 재료 및 그 특성 (Properties of Grout Material for Seawall Using Slags from Steel Making Industry)

  • 김하석;김기석 ;백봉현;육심훈
    • 한국건설순환자원학회논문집
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    • 제10권4호
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    • pp.523-530
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    • 2022
  • 해수의 흐름이 존재하는 방조제 제체 보강공사 시 가장 큰 문제는 재료의 유출로, 콘크리트의 중력식 타설은 원활히 공극을 채우기 어렵고 굳지 않은 콘크리트의 시멘트는 해수에 분산될 확률이 높기 때문에 경화 후 품질의 신뢰성이 저하될 뿐만 아니라, 인근 환경에 악역향을 끼칠 수 있다. 이에 주입이 가능한 겔형태의 주입재가 필요하다, 본 연구에서는 급결성을 띠는 전기로환원슬래그 및 고로슬래그를 활용하여 초기 강도와 해수 침지에 따른 내구성 향상 효과를 평가하였다, 실험 결과 실리카계 약액과의 반응을 통해 흐름성을 갖는 겔형태의 주입재 제조가 가능하였으며 겔의 흐름성은 배합에 따라 105~143 mm 수준으로 현장모사 시험을 통해 외부 유출 없이 공극을 채울 수 있어 현장 적용성을 확인하였다.

위험 요인 평가를 위한 FMEA의 일반 RPN 모형과 활용에 관한 연구 (A Study on the Common RPN Model of Failure Mode Evaluation Analysis(FMEA) and its Application for Risk Factor Evaluation)

  • 조성우;이한솔;강주영
    • 품질경영학회지
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    • 제50권1호
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    • pp.125-138
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    • 2022
  • Purpose: Failure Mode and Effect Analysis (FMEA) is a widely utilized technique to measure product reliability by identifying potential failure modes. Even though FMEA techniques have been studied, the form of Risk Priority Number (RPN) used to evaluate risk priority in FMEA is still questionable because of its shortcomings. In this study, we suggest common RPN(cRPN) to resolve shortcomings of the traditional RPN and show the extensibility of cRPN. Methods: We suggest cRPN which is based on Cobb-Douglas production function, and represent the various application on weighting risk factors, weighted RPN in a mathematical way, and show the possibility of statistical approach. We also conduct numerical study to examine the difference of the traditional RPN and cRPN as well as the potential application from the analysis on marginal effects of each risk factor. Results: cRPN successfully integrates previously suggested approaches especially on the relative importance of risk factors and weighting RPN. Moreover, we analyze the effect of corrective actions in terms of econometric analysis using cRPN. Since cRPN is rely on the reliable mathematical model, there would be numerous applications using cRPN such as smart factory based on A.I. techniques. Conclusion: We propose a reliable mathematical model of RPN based on Cobb-Douglas production function. Our suggested model, cRPN, resolves various shortcomings such as consideration of the relative importance, the effect of combinations among risk factors. In addition, by adopting a reliable mathematical model, quantitative approaches are expected to be applied using cRPN. We find that cRPN can be utilized to the field of industry because it is able to be applied without modifying the entire systems or the conventional actions.