• 한국식품영양과학회지
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• 제45권4호
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• pp.510-517
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• 2016

본 연구는 싸리나무 잎에서 분리한 flavonoid 화합물인 eriodictyol의 항산화 활성을 평가하기 위해 hydrogen peroxide로 산화적 스트레스를 유도한 HepG2 세포에서 eriodictyol 화합물의 처리가 SOD-1, SOD-2, CAT 및 GPx의 유전자 발현에 미치는 영향을 분석하였으며, 또한 간 기능 지표효소인 GOT, LDH 및 GGT 활성을 분석하였다. 그리고 세포 내 활성산소종 생성 억제 효능을 분석하기 위하여 DCFH-DA assay를 실시하여 eriodictyol 화합물의 기능성 소재로서의 가능성을 알아보고자 본 실험을 하였다. Eriodictyol 화합물의 세포독성을 확인하기 위하여 HepG2 세포주를 이용하여 실시한 결과 eriodictyol 화합물을 $10{\sim}50{\mu}g/mL$의 농도로 처리한 모든 실험군에서 약 98% 이상의 세포생존율을 나타내었다. 항산화 효소 유전자 발현량을 통한 산화스트레스 억제 효과를 분석하기 위하여 HepG2 세포주에 hydrogen peroxide를 처리하여 산화스트레스가 증가시킨 조건에서 eriodictyol 화합물을 처리하여 SOD-1, SOD-2, CAT 및 GPx 발현량을 분석한 결과 eriodictyol 화합물의 처리 농도가 증가할수록 hydrogen peroxide 처리에 의해 감소한 SOD-1, SOD-2, CAT 및 GPx 발현량이 유의적으로 증가하는 것을 확인할 수 있었다. 간 기능 지표효소 활성을 측정하기 위해 GOT, LDH 및 GGT 활성을 분석한 결과 hydrogen peroxide로 단독 처리한 대조군과 eriodictyol 화합물을 처리한 군을 비교하였을 때 eriodictyol 화합물을 처리한 군에서 hydrogen peroxide 처리에 의해 증가한 GOT, LDH 및 GGT 활성이 유의적으로 감소하였다. HepG2 세포주에 eriodictyol 화합물을 처리하여 세포 내 활성산소종 생성에 미치는 영향을 DCFH-DA assay로 확인한 결과 eriodictyol 화합물의 농도가 증가함에 따라 세포내 활성산소종의 생성을 억제하는 것을 확인할 수 있었다. 따라서 본 실험을 통하여 eriodictyol 화합물은 산화적 스트레스로부터 항산화 효소 활성을 증가시키며, 활성산소종의 생성을 억제하는 효과를 확인할 수 있었다. 또한 간 기능 지표효소의 활성을 감소시켜 세포 보호 효과를 나타내어 항산화 활성 및 세포 보호 효과를 나타내는 기능성 소재로써 이용 가능성이 높을 것으로 판단되며, 후속연구를 통해 싸리나무 유래 eriodictyol 화합물의 세포 내 항산화 단백질 발현에 미치는 영향 및 동물실험을 통한 항산화 효과를 검증하는 것이 필요할 것으로 생각된다.

• 한국유기농업학회지
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• 제23권4호
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• pp.939-950
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• 2015

The purpose of this study was to estimate the growth promoting effects and improvement of antioxidant activity of the soybean sprouts treated with Chlorella sp. culture solution. The soybean sprout treated with 0.1% and 0.2% Chlorella sp. culture solution was significantly increased the length (more than 43.0%), the thickness (more than 0.5~0.7 mm), fresh weight (more than 2.9~3.7 g) compared to non-treated control in vitro. In organic soybean sprouts farm, the 0.2% chlorella culture solution applied to mass culture of soybean sprout and the fresh weight of soybean sprouts increased by more than 25% and the yield was very high as 598.33% compared to untreated control. In addition of sensory test, there is no fishy odor and better crunchy texture and nutty flavor for the treatment soybean sprouts compared to untreated soybean sprouts. Particularly, free-radical scavenging activity (DPPH) and superoxide dismutase activity (SOD) of the soybean sprouts were significantly increased more than 26.1% and 40.4%, respectively by treated with 0.1% and 0.2% Chlorella culture solution. Consequently, the treatment of chlorella culture solution to grow soybean sprouts is also promoting quality and antioxidant activity as well as promoting the growth of sprouts. Therefore, chlorella is considered to be worth as functional materials for high-quality sprouts grown.

• Asian-Australasian Journal of Animal Sciences
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• 제29권6호
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• pp.814-822
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• 2016

This study was conducted to determine and compare the effects of yeast yeast culture (YC) and vitamin E (VE) supplementation on endotoxin absorption and antioxidant status in lactating dairy goats suffering from heat stress (HS). Three first lactation Saanen dairy goats (body weight $30{\pm}1.5kg$) were surgically fitted with indwelling catheters in the portal vein, mesenteric vein and carotid artery, and were randomly assigned to a $3{\times}3$ Latin square design. Dietary treatments were the basal diet, and the basal diet supplemented with either 100 IU VE or 30 g YC. Goats were kept in temperature and humidity-controlled room at $35^{\circ}C$ from 8:00 to 20:00 and at $24^{\circ}C$ from 20:00 till the next morning at 8:00. The relative humidity was kept at 55%. HS increased dairy goats' rectum temperature and respiration frequency (p<0.01). HS reduced plasma flux rate of milk goats (p<0.01), but the plasma flux rate increased when the animal was under the conditions of the thermo-neutral period (p<0.01). The VE supplementation lowered dairy goats' rectum temperature during thermo-neutral period (p<0.01). Meanwhile, no significant differences were observed between the control and YC treatment in rectum temperature and respiration frequency (p>0.05). Dietary supplementation of VE and YC reduced heat stressed dairy goats' endotoxin concentration of the carotid artery and portal vein (p<0.01). However, the endotoxin concentration of the YC treatment was higher than that of the VE treatment (p<0.01). Both VE and YC supplementation decreased heat stressed dairy goats' absorption of endotoxin in portal vein (p<0.01). The endotoxin absorption of YC treatment was higher than the VE treatment (p<0.01). The addition of VE and YC decreased dairy goats' superoxide dismutase (SOD) concentration during HS and the whole experiment period (p<0.01). The addition of VE lowered SOD concentration during thermo-neutral period (p<0.01). Likewise, the addition of VE and YC lowered dairy goats' malonaldehyde (MDA) concentration during HS and the whole experimental period, and the MDA concentration in the VE treatment was lower than the YC treatment (p<0.05). The addition of VE decreased MDA concentration during thermo-neutral period. On the contrast, the addition of VE increased dairy goats total antioxidant potential (TAP) concentration during HS, thermo-neutral and the whole experimental period (p<0.01). The addition of YC increased TAP concentration only during HS period (p<0.01). It is concluded that both VE and YC are useful in alleviating HS of dairy goats by weakening endotoxin absorption and promoting antioxidant capacity. Compared with YC, VE is much more powerful in easing dairy goats HS.

• International Journal of Advanced Culture Technology
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• 제8권1호
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• pp.226-235
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• 2020

In this paper, we present to evaluate physiological activity of Robinia pseudo-acacia leaf and its skin penetration using liposome and cell penetrating peptide. After extraction with Robinia pseudo-acacia leaf using the distilled water and supercritical, various physiological activities were examined. In antioxidants experiments, the total concentration of polyphenol compounds was determined to be 56.88 mg/g in hydrothermal extract, 45.07 mg/g in supercritical extract. The DPPH radical scavenging ability at 1,000 ㎍/mL was 33.97% in supercritical extract. The scavenging effect on SOD experiment at 500 ㎍/mL was 76.41% in supercritical extract. In the antimicrobial experiments, the hydrothermal extract had no effect, but supercritical extract represented maximum clear zone of 14.00 mm in Staphylococcus aureus strain. Liposome containing the RSE (Robinia pseudo-acacia leaf supercritical extract) reduced particle size and stabilized zeta potential. In the epidermal permeability experiment, it was confirmed that the permeation of liposome containing the RSE and cell penetrating peptides was remarkable.

• 생약학회지
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• 제33권4호통권131호
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• pp.376-383
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• 2002

The methanol extracts prepared from ten kinds of culinary herbs were investigated for the cytotoxcic effect againt L1210 cancer cells and the mode of action. The substantial cytotoxic effects were observed in all cases with the most prominent effect demonstrated by lemon verbena extract showing $87{\pm}4.1%$ cytotoxicity with $100{\mu}g/ml$ concentration and 3 days culture period. The cytotoxic effect was found to be dose and culture period dependent. With respect to the mechanism of the cytotoxicity, the augmented generation of $O_2{^-}ion$ and the dramatically escalated activities of antioxidant enzymes such as superoxide dismutase(SOD) and glutathione peroixdase (GPx) with addition of the herb methanol extractw suggested that there would be the involvement of reactive oxygen species (ROS) metabolism in the course of L1210 cancer cell death by the mothanol extract of the edible herbs.

• International Journal of Advanced Culture Technology
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• 제6권4호
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• pp.97-108
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• 2018

The present study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): group I-normal group, group II-DSS control group, group III-DSS + sulfasalazine (30 mg/kg), group IV-DSS + sulfasalazine (60 mg/kg), group V-DSS + sulfasalazine (30 mg/kg) + Radish Extract mixture (30 mg /kg) (SRE). DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling moleculesthrough suppression both mitogen-activated protein kinases(MAPK) and nuclear factor-kappa B ($NF-{\kappa}B$) signaling pathways, and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase. This is the first report that Radish extract mixture combined with sulfasalazine protects against experimental UC via the inhibition of both inflammation and apoptosis, very similar to the standard-of-care sulfasalazine.

• 한국식생활문화학회지
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• 제36권5호
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• pp.522-529
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• 2021

This study was conducted to analyze the antioxidant and antidiabetic activities of acorns according to the types of Quercus serrata Thunb (QST) and Q. acutissima Carruther (QAC). The total polyphenol contents of the extracts from QST and QAC were 220.59 and 320.96 mg GAE/g, respectively. The content of total polyphenol of QAC was higher than that of QAC (p<0.001). DPPH (2,2 Diphenyl 1 picrylhydrazyl) radical scavenging activity, reducing power and superoxide dismutase (SOD)-like activity were increased in a concentration-dependent manner by both acorn extracts, and QAC showed high activity in all antioxidant experiments (p<0.05). The inhibitory activities of α-glucosidase and α-amylase were also increased in a concentration-dependent manner, and QAC showed higher inhibitory activity than QST (p<0.05). Our study indicates that QST and QAC are functional food materials with high antioxidant and antidiabetic activities. In addition, QAC has a higher physiological activity than QST.

• 한국식품저장유통학회지
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• 제22권4호
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• pp.567-576
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• 2015

본 연구는 산지에 자생하는 참취와 밭에서 재배하는 참취의 품질과 기능성을 평가하기 위하여 에탄올 추출물의 항산화 물질 및 항산화성에 대하여 조사하였다. 참취의 에탄올 추출물의 수율은 블랜칭 건조한 시료에서 높았고, 폴리페놀 함량은 야생 및 재배 블랜칭 건조한 시료에서 각각 35.59, 26.83 mg/g으로 야생 조에서 높은 함량을 보였다. 전자공여능은 농도가 증가함에 따라 모든 군에서 활성이 증가했고 블랜칭 건조가 가장 높은 전자공여능을 보였다. SOD 유사활성능은 야생 참취는 생채에서 높은 활성을 보였고, 재배 참취는 블랜칭 건조에서 높았다. 아질산염 소거능 pH 1.2에서는 생채와 재배 추출물의 농도가 높을수록 높은 소거능을 보였고, 재배보다 생채 참취 추출물이 더 높았다. Xanthine oxidase 저해효과, tyrosinase 저해효과 측정 결과에서는 추출물의 농도가 증가할수록 저해효과가 높았으며 야생 참취에서 높은 저해활성을 보였다. 환원력은 추출물의 농도가 증가함에 따라 환원력은 증가함을 보였고 블랜칭 건조가 높은 환원력을 보였으며 야생 참취 추출물에서 높은 환원력을 나타내었다. 이상의 결과에서 참취에탄올 추출물의 항산화 활성은 재배 참취의 추출물에 비해 야생 참취의 추출물에서 높았다. 참취 추출물의 항산화 활성은 생채 및 블랜칭 건조 참취 추출물의 항산화 활성이 자연건조 참취 추출물에 비해 전반적으로 높았다. 따라서 참취는 영양성과 항산화 활성이 우수하여 항산화 및 노화예방에 우수한 식품이며, 참취를 이용한 다양한 가공식품의 개발은 국민 건강과 더불어 참취의 소비확대 및 부가가치 창출에 이바지할 수 있을 것으로 기대된다.

• 대한의생명과학회지
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• 제16권4호
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• pp.265-269
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• 2010

Puer tea is a traditional beverage originating from Yunnan area of China. We have analyzed 11 different commercial tea brands provided by Daboo Culture and Art Center. This study was carried out to evaluate the contents of polyphenols, antibacterial activity, antioxidantive ability and physiological activities of extracts from Puer tea. The electron donating ability was ranged from 57.26~99.16% and SOD-like activity was ranged from 1.4~10.4%. The inhibitory effect on the growth of cancer cell lines was examined by MIT assay. The Puer tea extract exhibited the greatest inhibitory effect at the concentration of 2% for all cancer cells tested.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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• pp.12-20
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• 1998

Primary neuronal culture was studied for observing effect of ginsenoside Rgl (Rgl) on serum-free medium induced apoptosis. Results showed that Rgl at concentration of 1 umol$.$ L-1 and 10 umol$.$L-1 could inhibit apoptosis, decrease intracellular calcium concentration in cultured cortical neurons, enhance SOD activity in both aged rat cortex and cultured cortical neurons, scavenge cytotoxic oxygen free radicals, decrease NO content and NOS activity in aged rat cortex and cultured cortical neurons, increase bel-2 gene expression in rat brain. These results provided new data for elucidating the anti-aging effect of Rgi. Rgl is considered to be a useful drug for treatment of Alzheimer's disease and brain aging.