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Cytotoxicity of Methanol Extract of Edible Herbs Against L1210 Cells with the Changes of Antioxidant Enzymes Activities  

Kim, Soo-Jin (Department of Chemistry, School of Natural Science Sangmyung University)
Cho, Yong-Sun (Department of Chemistry, School of Natural Science Sangmyung University)
Park, Sie-Won (Department of Chemistry, School of Natural Science Sangmyung University)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.4, 2002 , pp. 376-383 More about this Journal
Abstract
The methanol extracts prepared from ten kinds of culinary herbs were investigated for the cytotoxcic effect againt L1210 cancer cells and the mode of action. The substantial cytotoxic effects were observed in all cases with the most prominent effect demonstrated by lemon verbena extract showing $87{\pm}4.1%$ cytotoxicity with $100{\mu}g/ml$ concentration and 3 days culture period. The cytotoxic effect was found to be dose and culture period dependent. With respect to the mechanism of the cytotoxicity, the augmented generation of $O_2{^-}ion$ and the dramatically escalated activities of antioxidant enzymes such as superoxide dismutase(SOD) and glutathione peroixdase (GPx) with addition of the herb methanol extractw suggested that there would be the involvement of reactive oxygen species (ROS) metabolism in the course of L1210 cancer cell death by the mothanol extract of the edible herbs.
Keywords
Edible herbs; L1210 cells; Cytotoxicity; Reactive oxygen species (ROS); ${O_2}^{-}\; ion$; Superoxide dismutase; Glutathione peroxidse;
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  • Reference
1 Craig, W. J. (1999) Health promoting properties of common herbs. Am J. Clin. Nutr. 70: 491S-499S
2 Caragay, A. B. (1992) Cancer preventive foods and ingredients. Food Technol. 46: 65-68
3 Elson, C. E. and Yu, S. G. (1994) The chemoprevention of cancer by mevalonate derived constituents of fruits and vegetables. J. Nutr. 124: 607-614
4 National Cancer Institute USA: Cell culture screen KB. Protocol 1600. (1972) Cancer Chemotherapy Rep. (Part 3) 3: 17-27
5 Tyler, V. E. (1994) Herbs of Choice: The therapeutic use of phytomedicinals. pp: 126-145, Haworth Press. New York
6 Rice, E. C. and Burdon, R. Free radical lipid interaction and their pahological consequences. (1993) Prog. Lipid Res. 32: 71-78   DOI   ScienceOn
7 Lee, N. K., Kim, H. J., Yang, S. J., Kim, Y. S., Choi, H. I. Shim. M. J. and Kim, T. U. (2001) The anticncer mechanism of taxol diethylenetriamine pentaacetate conjugate in HT29 human colorectal cancer cells. J. Biochem. Mol. Biol. 34: 237-245
8 Lee, K. G., Mitchell, A. E. and Shibamoto, T. (2000) Determination of antioxidant properties of aroma extracts from various beans. J. Agr. Food. Chem. 43: c
9 Zheng, G. Q., Zhang, J., Kenney, P. M. and Lam, L. K. T. (1994) Stimulation of glutathione S-transferase and inhibition of carcinogenesis in mice by celery seed oil constitutents. In: Huang M. T., Osawa, T., Ho, C. T., Rosen, R. T., eds., Food phytochemicals for cancer prevention I. Fruits and vegetables, Washington, DC: American Chemical Society pp: 230-238
10 Kikuzaki, H. and Nakatani, N. (1993) Antioxidant effects of some ginger constituents. J. Food Sci. 58: 1407-1410   DOI   ScienceOn
11 Cemtti, P. A. (1994) Oxidative stress and cancer. Lancet 344: 862-869   DOI   ScienceOn
12 Bailer, J. C. and Gornick, H. L. (1997) Cancer undefeated. N. Eng. J. Med. 336: 1569-1573   DOI   ScienceOn
13 Tian, Q., Miller, E. G., Ahmad, H., Tang, L. and Patil, B. S. (2001) Differential inhibition of human cancer cell proliferdon by citrus limonoids. Nutrition and Cancer 40: 180-184   DOI   ScienceOn
14 Shacter, E., Williams, J. A., Hinson, R. M., Senturker, S. and Lee, Y. J. (2000) Oxidative stress interferes with cancer chemotherapy : inhibition of lymphoma cell apoptosis and phagocytosis. Blood 96: 307-313
15 Cuvlier, M. E., Bercet, C. and Richard, H. (1994) Antioxidant constituents in sage(Salvia officinales) J. Agr. Food Chem. 42: 665-669   DOI   ScienceOn
16 Kagan, V. E., Kuzmenko, A. I., Tyurina, Y. Y, Shvedora, A. A., Matrsura, T. and Yalowich, J. C. (2001) Pro-oxidant and antioxidant mechanism of etoposide in HL60 cells: Role of myelopereoxidase. Cancer Res. 61: 7777-7784
17 Roberfield, M. and Calderon, P. B. (1995) Free radicals and oxidative phenomenon in biological system, pp.: 48-63, Mareel Dekkor Inc. New York
18 Boyum, A. (1968) Isolation of leukocytes from human blood. Scan. J. Clin. Invest. 21: 9-14   DOI
19 Starway, A. (1982) Attenuadve Medicine ; A guide to natural therapies, pp: 18-32, Penguin Books Ltd. Hamondworth. New York
20 Eisenberg, D. M., Kessler, R.C., Foster, C., Norlock, F. E. Calkins D. R. and Delbanco, T. L. (1993) Unconventional medicine in the United States, preference, costs and patterns of use. N. Engl. J. Med. 328: 246-252   DOI   ScienceOn
21 McCord, J. and Fridovich, I. (1969) Superoxide dismutase. An enzymatic function for erythrocuprein (heterocuprein). J. Biol. Chem. 244: 6049-6055
22 Jung, D. Y. , Ha, H. K., Kim, A. N., Lee, S. M., Min, T. J. and Park, S. W. (2000) Cytotoxicity of SD-994 from Artemisia argyi against L1210 cells with concomitant induction of antioxidant enzymes. Yakhak Hoeji 44: 213-218
23 Thayer, P. S., Himmelfarb, P. and Watts, G. L. (1971) Cytotoxicity assays with L1210 cells in vitro: comparision with L1210 and KB cells in vitro. Cancer Chemostherapy Rep. (Part 2) 2: 1-25
24 Boyum, A. (1968) Isolation of leukocytes from human blood. Scan. J. Clin. Invest. 21: 9-16   DOI
25 Jacobson, M. D. (1996) Reactive oxygen species and programmed cell death. TIBS 21: 83-89
26 Balchin, M. L. (1997) Essential oils and 'aromatherapy': their modern role in healing. J. Rus. Soc. Health 117: 324-329   DOI
27 Bucke, J. (1999) Use of aromatherapy as a complementary treatment for chronic pain. Altern. Ther. 5: 42-51
28 Lam, L. K. T., Zhang, J., Hasegawa, S. and Chut, H. A. J. (1994) Inhibition of chemically induced carcinogenesis by citrus liminoids. In: Huang, M. T., Osawa, T., Ho, C. T., Rosen, R. T., eds. Food of phytochemicals for cancer prevention I. fruits and vegetables, Washington DC : American Chemical Society. pp: 209-219
29 Burger, A., Jenkins, T., Double, J. and Bibby, M. (1999) Cellular uptake, cytotoxicity and DNA binding studies of the novel imidazolacridine antineoplatic agent C1311. Br. J. Cancer 81: 367-373   DOI   ScienceOn
30 Markesbery, W. R. (1994) Oxidative stress hypothesis in Alzheimer disease. Free Radical Biology and Med. 23: 134-141
31 Lee, T. E., Park, S. W. and Min, T. J. (1999) Antiproliferative effect of Artemisia argyi extract against J774. A cells and subcellular superoxide dismutase (SOD) activity changes. J. Biochem. Molecular. Biol. 32: 585-592
32 Tyler, V. E. (1993) The Honest Herbal, 3rd edn. pp: 57-73, Haworth Press Inc. Binghampton. New York
33 Wieseman, H. and Halliwell, B. (1996) Damage to DNA by reactive oxygen species and nitrogen species: role in inflammatory disease and progression to cancer. Biochem. J. 313: 17-29   DOI
34 Astrow, A. B. (1994) Rethinking cancer. Lancet 343: 494-498   DOI   ScienceOn
35 Evans, M. D., Griffith, H. R. and Lunec, J. (1997) Reactive oxygen species and their cytotoxic mechanisms. Advan. Motecul. Cett Biol. 20: 25-32