International Journal of Advanced Culture Technology

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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Antimicrobial Effect of Supercritical Robinia pseudo-acacia Leaf Extracts and Its Transdermal Delivery System with Cell Penetrating Peptide

  • Heo, Soo Hyeon (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Park, Su In (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Lee, Jinseo (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Kim, Miok (Department of Beauty Design, Shin Ansan Univ.) ;
  • Shin, Moon Sam (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.)
  • Received : 2020.01.18
  • Accepted : 2020.02.20
  • Published : 2020.03.31
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Abstract

In this paper, we present to evaluate physiological activity of Robinia pseudo-acacia leaf and its skin penetration using liposome and cell penetrating peptide. After extraction with Robinia pseudo-acacia leaf using the distilled water and supercritical, various physiological activities were examined. In antioxidants experiments, the total concentration of polyphenol compounds was determined to be 56.88 mg/g in hydrothermal extract, 45.07 mg/g in supercritical extract. The DPPH radical scavenging ability at 1,000 ㎍/mL was 33.97% in supercritical extract. The scavenging effect on SOD experiment at 500 ㎍/mL was 76.41% in supercritical extract. In the antimicrobial experiments, the hydrothermal extract had no effect, but supercritical extract represented maximum clear zone of 14.00 mm in Staphylococcus aureus strain. Liposome containing the RSE (Robinia pseudo-acacia leaf supercritical extract) reduced particle size and stabilized zeta potential. In the epidermal permeability experiment, it was confirmed that the permeation of liposome containing the RSE and cell penetrating peptides was remarkable.

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References

  1. D.W. Thayer, G. Boyd, A. Kim, J.B. Fox and H.M. Farrel, "Fate of gamma-irradiated Listeria monocytogenes during refrigerated storage on raw of cooked turkey breast meat", Journal of Food Protection, Vol. 61, No. 8, pp. 979-987, 1998. DOI: https://doi.org/10.4315/0362-028X-61.8.979
  2. S.I. Kreydiyyeh and J. Usta, "Diuretic effect and mechanism of action of parsley", Journal of Ethnopharmacology, Vol. 79, No. 3, pp. 353-357, 2002. DOI: https://doi.org/10.1016/S0378-8741(01)00408-1
  3. C.S. Kim, K.S. Ahn, K.C. Perk, B.S. Jeon, J.T. Lee and J.W. Park, "Isolation of antioxidative compound from Scutellaria baicalensis", Korean Journal of Medicinal Crop Science, Vol. 14, No. 4, pp. 212-216, 2006. DOI: http://kmbase.medric.or.kr/KMID/1098420060140040212
  4. A.D. Banham, M.M. Standish and J.C. Watkins, "Diffusion of univalent ions across the lamellae of swollen phospholipids", Journal of Molecular Biology, Vol. 13, No. 1, pp. 238-252, 1965. DOI: https://doi.org/10.1016/s0022-2836(65)80093-6
  5. L.D. Mayer, M.B. Bally, K.J. Hope and P.R. Cullis, "Techniques for encapsulating bioacticve agents into liposomes", Chemistry and Physics of Lipids, Vol. 40, pp. 333-345, 1986. DOI: https://doi.org/10.1016/0009-3084(86)90077-0
  6. A. Gabizon, F.H.D. Roerdink and Kroon, "Liposomes as a drug delivery system in cancer chemotheraphy", Horizons Biochemistry and Biophysics, Vol. 9, pp. 185-211, 1989.
  7. E. Mayhew, R. Lazo, W.J. Vail, J. King and A.M. Green, "Characterization of liposomes prepared using a micro emulsifier", Biochimica et Biophysica Acta, Vol. 775, pp. 169-174, 1984. DOI: https://doi.org/10.1016/0005-2736(84)90167-6
  8. E. Mayhew, S. Conroy, J. King, R. Razo, G. Nikolopoulus, A. Sciliano and W.J. Vail, "High-pressure continuous-flow system for drug entrapment in liposomes", Methods in Enzymology, Vol. 149, pp. 64-77, 1987. DOI: https://doi.org/10.1016/0076-6879(87)49044-7
  9. P.A. Wender, D.J. Mitchell, K. Pattabiraman, E.T. Pelkey, L. Steinman and J.B. Rothbard, "The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: peptoid molecular transporters", Proceedings of the National Academy of Sciences, Vol. 97, pp. 13003-13008, 2000. DOI: https://doi.org/10.1073/pnas.97.24.13003
  10. G.M. An, S.I. Park, and M.S. Shin, "Cosmetic efficacy of Red Pinus densiflora and its epidermis penetration with polymer micelle and cell penetrating peptide", The Internal Journal of Advanced Cultural Technology, Vol. 7, No. 3, pp. 10-24, 2019. DOI: https://doi.org/10.17703/IJACT.2019.7.3.10
  11. O. Folin and W. Denis, "On phosphotungstic-phosphomolybdic compounds as color reagents", Journal of Biological Chemistry, Vol. 12, pp. 239-243, 1912. https://doi.org/10.1016/S0021-9258(18)88697-5
  12. M. S. Blois, "Antioxidant determinations by the use of a stable free radical", Nature, Vol. 181, pp. 1199-1200, 1958. DOI: https://doi.org/10.1038/1811199a0
  13. S. Marklund and G. Marklund, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", European Journal of Biochemistry, Vol. 47, No. 3, pp. 469-474, 1974. DOI: https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  14. M.O. Ko, H.J. Kang, J.H. Hwang and K.W. Yang, "Screening of the antibacterial effects by ethanol extracts from natural plant in Jeju against Propionibacterium acnes", Journal of Society of Cosmetic Scientists of Korea, Vol. 44, No. 1, pp. 59-66, 2018. DOI: https://doi.org/10.15230/SCSK.2018.44.1.59
  15. S. Shin, "Biological activities on phenolic compounds of Japanese anise (Illicium anisatum L) extracts", The Internal Journal of Advanced Cultural Technology, Vol. 7, No. 3, pp. 120-125, 2019. DOI: https://doi.org/10.17703/IJACT.2019.7.3.120
  16. J.G. Kim and J.H. Son, "The quantity of superoxide dismutase (SOD) in fresh royal jelly", Korea Beekeeping Association, Vol. 11, No. 1, pp. 8-12, 1996.
  17. G.M. An, S.I. Park, M.G. Kim and M.S. Shin, "Antioxidant, antimicrobial and anti-inflammatory effects of Anemarrhena asphodeloides extracts using supercritical Extraction", Journal of Investigative Cosmetology, Vol. 14, No. 4, pp. 455-462, 2018. DOI: https://doi.org/10.15810/jic.2018.14.4.007
  18. S.H. Heo, S.I. Park, G.M. An, M.G. Kim and M.S. Shin, "Physiological activity of Robinia pseudo acacia leaf extracts and enhancement of skin permeation using polymer micelles and cell penetrating peptide", The Journal of the Convergence on Culture Technology, Vol. 5 No. 3, pp. 271-282, 2019 DOI : https://doi.org/10.17703/JCCT.2019.5.3.271