• 대한본초학회지
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• 제31권5호
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• pp.41-46
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• 2016

Objectives : The Skin is composed of multiple layers, including the epidermis, dermis, and hypodermis. It provides a vital barrier structure that protects vertebrates from external environmental antigens, solvents, ultraviolet light, microorganisms, toxins, and weather conditions. Although several biological effects of Mori Follium have been reported, beneficial effects of Mori Follium in skin health remain unclear. In this study, we prepared water extract of Mori Follium (MLE) and evaluated the effects on melanin accumulation and expression levels of skin fibril-related proteins.Methods : The cytotoxicities of MLE in B16F10 melanoma and human skin fibroblasts (HSF) were examined by MTT assay. Inhibitory effect of MLE on the α-MSH- and IBMX-induced melanosis in B16F10 melanoma was examined. The expression levels of fibronectin, collagen 1α2, and CCN2 in MLE-treated HSF were analyzed by reverse transcription-polymer chain reaction (RT-PCR) and western blotting.Results : The MLE treatment for 24 h did not affect to the B16F10 and HSF at concentrations of 1, 10, 50, 100, 200, 400 and 800 ㎍/ml. The MLE treatment for 72 h significantly and dose dependently suppressed melanin accumulation in B16F10 melanoma. In addition, the MLE treatment up-regulated expression levels of skin fibril-related genes such as fibronectin, collagen 1α2, and CCN2 in HSF. Our western blot analysis revealed MLE-induced up-regulation of skin fibril-related genes required the activation of CCN2 protein.Conclusions : In conclusion, these findings suggest that the MLE could be used in development of cosmetic natural material of maintaining healthy skin.

• 대한한의학회지
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• 제37권4호
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• pp.1-9
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• 2016

Objectives: The aim of this study was to solve skin moisturizing action mechanism issues of pomegranate concentrated solution (PCS) and dried pomegranate concentrate powder (PCP), at least partially. Materials and methods: In this study, ceramide and $TGF-{\beta}1$ contents with $TGF-{\beta}1$ mRNA expressions were analysis on the skin tissue homogenate samples after 56 days of continuous oral administration of PCS 1, 2, and 4 ml/kg, and PCP 100, 200 and 400 mg/kg. Results: Noticeable and dose-dependent increases of skin $TGF-{\beta}1$ contents and mRNA expressions were demonstrated in all PCP and PCS treated mice as compared with intact vehicle control, but no significant changes on the skin ceramide contents were demonstrated in all PCP and PCS treated mice as compared with intact vehicle control, in the current study. In addition, PCP 200 mg/kg showed similar increases of the skin $TGF-{\beta}1$ contents and mRNA expressions as compared to those of PCS 4 ml/kg. Conclusions: The presented results suggested that in vivo skin moisturizing effects of PCP and PCS after oral administration through up regulation of hyaluronan synthesis demonstrated in our previous results, may be possibly mediated by modulation of $TGF-{\beta}1$ expressions at least partially, without critical influences on the skin ceramide contents.

• Environmental Analysis Health and Toxicology
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• 제23권1호
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• pp.63-65
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• 2008

The tissue distribution of $TiO_2$, nanopaprticles was investigated in mice after oral administration, and skin treatment. Male mice were treated with the dose of 5 g/kg of $TiO_2$ for three consecutive days and sacrificed at 24 hours after the last administration. As results, the orally administered $TiO_2$ nanoparticels were shown to be distributed in the testis, lung, and brain at 24 hours after the last treatment. Kidney does not seem to be the main target of $TiO_2$ nanoparticle distribution. It means that $TiO_2$ nanoparticles (17 nm) are easily absorbed through entero-gastric system and may cause toxicity in brain, lung, and reproductive organs. The distribution of skin treatment showed the same pattern like oral administration.

• 고신대학교 의과대학 학술지
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• 제33권2호
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• pp.245-251
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• 2018

Prophylactic antibiotics that are used to prevent post-operative infection can commonly cause anaphylactic reactions during anesthesia. It is therefore necessary to perform a skin test before antibiotics are administered in order to diagnose and prevent anaphylactic reactions. However, the results of the antibiotic skin test can differ according to the drug, dose, and reagent concentration. We report a case of anaphylactic shock with bronchospasm and cardiovascular collapse immediately following administration of the prophylactic cefazedone after induction of general anesthesia for laparoscopic cholecystectomy.

• 대한방사선치료학회지
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• 제17권1호
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• pp.33-40
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• 2005

목적 : 3D conformal, IMRT와 같은 치료기술의 개발로 인하여 CT를 이용한 최신의 치료들이 적용되어지고 있는 것이 지금의 현실이지만, 유방암 환자에서는 일반 CT의 hole size의 제약으로 인하여 일부의 경우는 기존의 환자고 정기구들이 사용되어지지 못하고 있다. 또한 simulation 시에 수술한 팔을 장시간 올리고 있는 자세를 유지함으로써 환자들이 pain을 호소하고, SCL (supraclavicular Imphnodes) 치료 시에 skin reaction의 문제점이 제기되었다. 이에 본원에서는 이러한 문제점을 개선하기 위하여 Ewha Breast device (EBD)를 자체 제작하여 사용하였으며, 이번 논제에서는 EBD의 제작과 유용성의 평가에 대하여 논하고자 한다. 대상 및 방법 : CT data를 이용하여 머리의 크기를 분석하였으며, 일반 CT의 hole size, 자세의 안정성, SCL field에서의 피부의 겹침(fold) 등을 고려하여 아크릴로 device를 제작하였으며 기존의 device를 사용하였을 경우와 set-up의 재현성, RTP상의 dose분포, SCL field에서의 skin reaction 등을 비교 분석하여 EBD의 유용성에 대하여 평가하였다. 결과 : 제작한 EBD를 사용 시 CT simulator가 아닌 hole size가 작은 일반 CT에서도 팔의 부딪힘이 없이 자세를 유지할 수 있었고, 위로 올린 팔의 pain도 많이 감소하였다. 그리고, 기존의 device와 비교하였을 경우에 set-up의 재현성에서 큰 차이를 보이지 않았으며, RTP상에서는 3D segment field를 이용한 plan으로 device의 tilting이 되지 않은 문제점은 해결할 수 있었으나 기존보다 팔을 많이 위로 올리지 않는 관계로 axillary region에서 이전보다 약$10\%$ 정도의 선량이 더 들어감을 알 수 있었다. SCL field에서의 skin reaction은 접힌 부분(fold)을 펼치기 위해 팔을 조정하여 위치를 잡는 관계로 이전보다 월등히 적은 skin reaction이 발생함을 알 수 있었다. 결론 : 비용이 저렴한 EBD의 제작으로 유방암 환자에서 CT를 이용한 3D 치료가 용이하게 되었으며, simulation 시에 팔의 pain을 경감시킬 수 있었고, skin reaction의 감소 등으로 치료에 EBD를 유용하게 사용할 수 있었다. 앞으로 EBD를 사용하여 나타난 device의 문제점 등을 보완 수정한다면 치료에 더 많은 도움이 되리라 생각된다.

• 대한디지털의료영상학회논문지
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• 제14권2호
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• pp.15-22
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• 2012

The purpose of this study was to measure the absorbed dose and calculate the effective dose for cone beam computed tomography (CBCT) and panorama units and to estimate usefulness of x-ray protective. Rando phantom and glass dosimeters were used for dosimetry. The absorbed doses were measured at 15 organs and 14 remainder from correspond to ICRP 2007 recommendations. The absorbed dose was highest in salivary glands as measured CBCT 2.420mGy, panorama 0.307mGy. Absorbed dose in another organs were high in order of thyroid, brain, skin, esophagus. The effective dose was CBCT 0.100mSv, panorama 0.011mSv and effective dose of panorama was higher than that of CBCT by 10 times. In case of wearing x-ray protective, reducing effective dose of CBCT by 0.066mSv (66%) and panorama by 0.008mSv (72%). Effective dose were reduced by radiological shielding but it needs further optimization studies, where dosimetric data are analyzed in combination with image quality with keep the patients' exposure as low as possible.

• 대한방사선치료학회지
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• 제17권1호
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• pp.57-71
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• 2005

목적 : 전자선을 이용하여 전신피부를 치료할 경우, 조사되는 피부선량과 정확도를 열형광 선량계(TLD)와 반도체(Diode) 검출기를 이용하여 평가하고자 한다. 대상 및 방법 : 본원에 내원한 다발성 발적을 보이는 균상식육종(Mycosis fungoides) 환자를 대상으로 스텐포드 테크닉(Stanford technique)을 시행하였다. 선형가속기의 6MeV 전자선을 SSD 300 cm에서 0.8 cm의 아크릴 스포일러를 사용하여 6개 자세로 각 자세 당 gantry각도 $64^{\circ}.\;90^{\circ}.\;116^{\circ}$로 각각 조사하면서 열형광 선량계와 반도체검출기를 이용하여 환자피부선량과 출력을 동시에 $5{\sim}6$회 측정하였다. 결과 : 열형광선량계의 측정결과 종양선량과 오차는 조사중심부(C.A) $+\;6\%$, 대퇴부(thigh) $+\;8\%$, 배꼽(umbilicus) $+\;4\%$, 종아리(calf) $-\;8\%$, 두정부(vertex) $-\;74.4\%$, 액와부(deep axillae) $-\;10.2\%$, 항문(anus)과 고환(testis) $-\;87\%$, 발바닥(sole) $-\;86\%$로 나타났다. 손톱과 발톱은 4 mm납으로 차폐하여 $+\;4\%$로 나타났다. 반도체검출기의 측정결과 선량오차는 환자조사중심부에서 $-\;4.5\%{\sim}+\;5\%$, 스포일러에 부착한 경우 $-\;1.1\%{\sim}+\;1\%$로 나타났다. 결론 : 열형광선량계의 측정결과, 전신피부의 선량오차는 $+\;8\%{\sim}-\;8\%$로 허용오차인 ${\pm}10\%$의 범위를 만족시키는 선량분포를 보였으며, 저선량 부위에는 여러 개의 열형광선량계로 측정하여 추가선량과 조사야 크기를 결정하여 두정부, 회음부, 양쪽 발바닥에 추가조사 하였다. 스포일러에서 측정한 전자선출력은 안정되었고, 환자조사 중심부에서 측정한 반도체 검출기의 선량오차는 검출기 부착위치재현과 환자자세재현 등에 의해 기인된 것으로 사료된다.

• 혜화의학회지
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• 제28권2호
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• pp.41-47
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• 2019

Objectives : Atopic dermatitis is an irritable skin disease accompanying rash and itching leading to impaired skin barrier. ATO-ALL is an ethanol extract of natural products comprising 12 herbs and effective on atopic dermatitis. In this study, we aimed to propose that the effect of ATO-ALL on skin regeneration in human keratinocyte cell line, HaCaT cells. Methods : To evaluate the skin regenerating effects of ATO-ALL, scratch wound healing assay, bromodeoxyuridine (BrdU) assay, and propidum iodide (PI) assay were performed using cultured HaCaT cell line. Result : Scratch wound healing assay showed that ATO-ALL was able to enhance the gap filling activity more than 2-fold at 7 ppm concentration compared with control group. BrdU assay demonstrated that ATO-ALL treatment increased the de novo cell proliferation in a dose-dependent manner. Finally, PI assay indicated that the cell cycle of HaCaT cells was modulated by ATO-ALL treatment. Conclusions : These results suggested that ATO-ALL may have skin regenerating effects by increasing cell proliferation via cell cycle regulation. Taken together, ATO-ALL is supposed to have a potential on regeneration of damaged skin or functional disease including atopic dermatitis.

• 대한한의학회지
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• 제32권2호
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• pp.1-13
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• 2011

Background and Objective: Coptidis Rhizoma is a medicinal herb known for its antioxidant and anti-inflammatory effect. The purpose of this study was to examine the effects of CR on the experimental burn elicitation in vitro and in vivo. Material and Methods: In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase (SOD) activity was measured. In vitro, the RAW 264.7 macrophage cells were treated with lipopolysaccharides for experimental inflammation. iNOS mRNA expression was observed after CR-treatment. In order to know effects on the skin regeneration in the burned mice, we counted the nitric oxide (NO) in blood. We also observed the histological structure in the epidermal basal layer and the dermal section, and we studied changes of angiogenesis in the capillaries surrounding the basal layer and dermal papilla. The changes of transcription of iNOS mRNA (inducible nitric oxide synthase mRNA) and changes of NF-${\kappa}$B (nuclear factor ${\kappa}$B) p65 positive reaction were also observed to investigate the changes of the stress in the skin. Results: The results indicated that CR has significant effects on the antioxidant effect on skin cells of mice after burn elicitation by increasing SOD activity in the in vitro test. It seemed that CR decreased the amount of NF-${\kappa}$B which induced the iNOS mRNA dose-dependently and suppress activating NO and angiogenesis. Furthermore, CR facilitated the process of skin recovery after experimental burn. Conclusion: CR can be applied for burned skin via antioxidant effect and skin regeneration.

• 대한본초학회지
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• 제34권2호
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• pp.75-82
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• 2019

Objectives : In this study, various biological effects of Curcuma longa L. have been studied, however, beneficial effect of Curcuma longa L. in skin health remain still unclear. In this study, Curcuma longa L. water extract (CLE) was prepared. Inhibitory effect of CLE on melanin accumulation of B16F10 cells and expression levels of skin fibril-related proteins of human skin fibroblasts (HSF) were evaluated. Methods : The cytotoxic effect of CLE in B16F10 cells and HSF were examined by MTT assay. Inhibitory effect of CLE on the ${\alpha}-MSH-$ and IBMX-induced melanin accumulation and tyrosinase activity were evaluated in B16F10 cells. The expression levels of connective tissue growth factor (CCN2), Smad2, procollagen $1{\alpha}2$, collagen $1{\alpha}2$, and fibronectin in CLE-treated HSF were analyzed by western blotting. Results : The CLE treatment (concentrations 10 to $400{\mu}g/ml$) for 72 h did not affect to the B16F10 viability. However, 200 and $400{\mu}g/ml$ of CLE treatment for 24 h showed cytotoxic effect in HSF. Therefore, the concentrations 10, 50, and $100{\mu}g/ml$ of CLE were chosen in this study. The CLE treatment for 72 h dose dependently and significantly suppressed melanin accumulation and tyrosinase activity of B16F10 cells. In addition, the CLE treatment up-regulated expression levels of skin fibril-related proteins such as CCN2, Smad2, procollagen $1{\alpha}2$, collagen $1{\alpha}2$, and fibronectin. Conclusions : In conclusion, these results suggest that the CLE could be used as a natural material for skin health.