Journal of Haehwa Medicine (혜화의학회지)

Research Institute of Korean Medicine (대전대학교 한의학연구소)
권호 표지

The effects of ATO-ALL on skin regeneration in human epidermal keratinocytes, HaCaT cells

ATO-ALL이 인간유래 피부 각질형성세포인 HaCaT 세포에서 피부재생에 미치는 효과

  • Shin, Younmin (Laboratory of Molecular Medicine, College of Korean Medicine, Daejeon University) ;
  • Lee, Won-Kil (Laboratory of Molecular Medicine, College of Korean Medicine, Daejeon University) ;
  • Kim, Seung Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Choi, Jeong June (Laboratory of Molecular Medicine, College of Korean Medicine, Daejeon University)
  • 신윤민 (대전대학교 한의과대학 분자의학실험실) ;
  • 이원길 (대전대학교 한의과대학 분자의학실험실) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 최정준 (대전대학교 한의과대학 분자의학실험실)
  • Received : 2019.08.26
  • Accepted : 2019.09.23
  • Published : 2019.09.21
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Abstract

Objectives : Atopic dermatitis is an irritable skin disease accompanying rash and itching leading to impaired skin barrier. ATO-ALL is an ethanol extract of natural products comprising 12 herbs and effective on atopic dermatitis. In this study, we aimed to propose that the effect of ATO-ALL on skin regeneration in human keratinocyte cell line, HaCaT cells. Methods : To evaluate the skin regenerating effects of ATO-ALL, scratch wound healing assay, bromodeoxyuridine (BrdU) assay, and propidum iodide (PI) assay were performed using cultured HaCaT cell line. Result : Scratch wound healing assay showed that ATO-ALL was able to enhance the gap filling activity more than 2-fold at 7 ppm concentration compared with control group. BrdU assay demonstrated that ATO-ALL treatment increased the de novo cell proliferation in a dose-dependent manner. Finally, PI assay indicated that the cell cycle of HaCaT cells was modulated by ATO-ALL treatment. Conclusions : These results suggested that ATO-ALL may have skin regenerating effects by increasing cell proliferation via cell cycle regulation. Taken together, ATO-ALL is supposed to have a potential on regeneration of damaged skin or functional disease including atopic dermatitis.

Keywords

References

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