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http://dx.doi.org/10.6116/kjh.2016.31.5.41.

Effect of Mori Follium Extract on the Melanogenesis and Skin Fibril Matrix  

Kwon, O Jun (Gyeongbuk Institute For Regional Program Evaluation)
Publication Information
The Korea Journal of Herbology / v.31, no.5, 2016 , pp. 41-46 More about this Journal
Abstract
Objectives : The Skin is composed of multiple layers, including the epidermis, dermis, and hypodermis. It provides a vital barrier structure that protects vertebrates from external environmental antigens, solvents, ultraviolet light, microorganisms, toxins, and weather conditions. Although several biological effects of Mori Follium have been reported, beneficial effects of Mori Follium in skin health remain unclear. In this study, we prepared water extract of Mori Follium (MLE) and evaluated the effects on melanin accumulation and expression levels of skin fibril-related proteins.Methods : The cytotoxicities of MLE in B16F10 melanoma and human skin fibroblasts (HSF) were examined by MTT assay. Inhibitory effect of MLE on the α-MSH- and IBMX-induced melanosis in B16F10 melanoma was examined. The expression levels of fibronectin, collagen 1α2, and CCN2 in MLE-treated HSF were analyzed by reverse transcription-polymer chain reaction (RT-PCR) and western blotting.Results : The MLE treatment for 24 h did not affect to the B16F10 and HSF at concentrations of 1, 10, 50, 100, 200, 400 and 800 ㎍/ml. The MLE treatment for 72 h significantly and dose dependently suppressed melanin accumulation in B16F10 melanoma. In addition, the MLE treatment up-regulated expression levels of skin fibril-related genes such as fibronectin, collagen 1α2, and CCN2 in HSF. Our western blot analysis revealed MLE-induced up-regulation of skin fibril-related genes required the activation of CCN2 protein.Conclusions : In conclusion, these findings suggest that the MLE could be used in development of cosmetic natural material of maintaining healthy skin.
Keywords
Mori Follium; melanogenesis; melanin; skin; depigmentation;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Shon MS, Kim RH, Kwon OJ, Roh SS, Kim GN. Beneficial roel and function of fisetin in skin health via regulation of the CCN2/TGF-β signaling pathway. Food Sci Biotechnol. 2016 ; 25 : 133-41.   DOI
2 Alexander PK, John TC, Asa HB. 3D nanomechanical evaluations of dermal structures in skin. J Mech Behav Biomed. 2016 ; 57 : 14-23.   DOI
3 Mariana D, Argirova, Beryl JO. Activation of protein-bound copper ions during early glycation: study on two proteins. Arch Biochem Biophys. 2003 ; 420 : 176-184.   DOI
4 Esther B, Henk FJH, Len R. Nutritional skin care: health effects of micronutrients and fatty acids1-3. Am J Clin Nutr. 2001 ; 73 : 853-64.   DOI
5 Magnus SA, Reinhild S, Lise HC, Ursula M. Tumor necrosis factor-α-accelerated degradation of type Ⅰ collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo. Eur J Cell Biol. 2015 ; 94 : 12-21.   DOI
6 Ana PCS, Carissa AG, Eduardo AGR, Renan FT, Ludmila FV, Tuania SC, Maria CTC, Antonio LBP, Jean NS. Infrared LED light therapy influences the expression of fibronectin and tenascin in skin wounds of malnourished rats-A preliminary study. Acta Histochem. 2014 ; 116 : 1185-91.   DOI
7 Liu LK, Chou FP, Chen YC, Ho HH, Wang CJ. Effects of mulberry (Morus alba L.) extracts on lipid homeostasis in vitro and in vivo. J Agric Food Chem. 2009 ; 57(16) : 7605-11.   DOI
8 Usui T, Takase M, Kaji Y, Suzuki K, Ishida K, Tsuru T, Miyata K, Kawabata M, Yamashita H. Extracellular matrix production regulation by TGF-beta in corneal endothelial cells. Invest Ophthal Vis Sci. 1988 ; 39 : 1981-9.
9 Samad F, Yamamoto K, Pandey M, Loskutoff DJ. Elevated expression of transforming growth factorbeta in adipose tissue from obese mice. Mol Med. 1997 ; 3 : 37-48.
10 Naowaboot J, Pannangpetch P, Kukonqviriyapan V, Kongyingyoes B, Kukonqviriyapan U. Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract in streptozotocin-induced chronic diabetic rats. Plant Foods Hum Nutr. 2009 ; 64 : 116-21.   DOI
11 Yoneta A, Yamashita T, Jin YH, Kondo S, Jimbow K. Ectopic expression of tyrosinase increases melanin synthesis and cell death following UVB irradiation in fibroblasts from familial atypical multiple mole and melanoma (FAMMM) patients. Melanoma Res. 2004 ; 14 : 387-94.   DOI
12 Jang MJ, Rhee SJ. Hypoglycemic effects of pills made of mulberry leaves and silkworm powder in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr. 2004 ; 33(10) : 1611-7.   DOI
13 Kim SY, Ryu KS, Lee WC, Ku HO, Lee HS, Lee KR. Hypoglycemic effect of mulberry leaves with anaerobic treatment in alloxan-induced diabetic mice. Kor J Pharmacogn. 1999 ; 30(2) : 123-9.
14 Kim J, Lee CW, Kim EK, Lee SJ, Park NH, Kim HS, Kim HK, Char K, Jang YP, Kim JW. Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts. J Ethnopharmacol. 2011 ; 137 : 427-33.   DOI
15 Urabe K, Aroca P, Tsukamoto K, Mascagna D, Paulumbo A, Prata G, Hearing VJ. The inherent cytotoxicity of melanin precursors. Biochim Biophys Acta. 1994 ; 1221 : 272-8.   DOI
16 Samad F, Yamamoto K, Pandey M, Loskutoff DJ. Elevated expression of transforming growth factorbeta in adipose tissue from obese mice. Mol Med. 1997 ; 3 : 37-48.
17 Kameyama K, Takemura T, Hamada Y, Sakai C, Kondoh S, Nishi-yama S. Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1 (TRP1), DOPAchrome tautomerase (TRP2) and a melanogenic inhibitor. J Invest Dermatol. 1993 ; 100 : 126-32.   DOI
18 Kim MJ, Ryu MJ. Inhibition of melanogenesis and anti-UV properties Reynoutria elliptica. Kor J Aesthet Cosmetol. 2012 ; 10 : 961-8.
19 Kang JR, Yoo SM. A study on effect on skin whitening action in Taraxanum platycarpum factions. Kor J Aesthet Cosmetol. 2009 ; 7 : 199-205.