The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Skin tissue homogenate analysis for ceramide and TGF-β1 contents with TGF-β1 mRNA expressions after treatment of pomegranate concentrated solution and dried pomegranate concentrate powder in mice

  • Hu, Jin-Ryul (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University) ;
  • Choi, Beom-Rak (Research Institute, HL Science Co., Ltd.) ;
  • Park, Hye-Rim (Research Institute, HL Science Co., Ltd.) ;
  • Sung, Mi-Sun (Research Institute, HL Science Co., Ltd.) ;
  • Yi, Hae-Yeon (Research Institute, HL Science Co., Ltd.) ;
  • Kang, Su-Jin (The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University) ;
  • Ku, Sae-Kwang (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Young-Joon (The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University)
  • Received : 2016.08.15
  • Accepted : 2016.12.15
  • Published : 2016.12.31
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Abstract

Objectives: The aim of this study was to solve skin moisturizing action mechanism issues of pomegranate concentrated solution (PCS) and dried pomegranate concentrate powder (PCP), at least partially. Materials and methods: In this study, ceramide and $TGF-{\beta}1$ contents with $TGF-{\beta}1$ mRNA expressions were analysis on the skin tissue homogenate samples after 56 days of continuous oral administration of PCS 1, 2, and 4 ml/kg, and PCP 100, 200 and 400 mg/kg. Results: Noticeable and dose-dependent increases of skin $TGF-{\beta}1$ contents and mRNA expressions were demonstrated in all PCP and PCS treated mice as compared with intact vehicle control, but no significant changes on the skin ceramide contents were demonstrated in all PCP and PCS treated mice as compared with intact vehicle control, in the current study. In addition, PCP 200 mg/kg showed similar increases of the skin $TGF-{\beta}1$ contents and mRNA expressions as compared to those of PCS 4 ml/kg. Conclusions: The presented results suggested that in vivo skin moisturizing effects of PCP and PCS after oral administration through up regulation of hyaluronan synthesis demonstrated in our previous results, may be possibly mediated by modulation of $TGF-{\beta}1$ expressions at least partially, without critical influences on the skin ceramide contents.

Keywords

References

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