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The effects of ATO-ALL on skin regeneration in human epidermal keratinocytes, HaCaT cells  

Shin, Younmin (Laboratory of Molecular Medicine, College of Korean Medicine, Daejeon University)
Lee, Won-Kil (Laboratory of Molecular Medicine, College of Korean Medicine, Daejeon University)
Kim, Seung Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University)
Choi, Jeong June (Laboratory of Molecular Medicine, College of Korean Medicine, Daejeon University)
Publication Information
Journal of Haehwa Medicine / v.28, no.2, 2019 , pp. 41-47 More about this Journal
Abstract
Objectives : Atopic dermatitis is an irritable skin disease accompanying rash and itching leading to impaired skin barrier. ATO-ALL is an ethanol extract of natural products comprising 12 herbs and effective on atopic dermatitis. In this study, we aimed to propose that the effect of ATO-ALL on skin regeneration in human keratinocyte cell line, HaCaT cells. Methods : To evaluate the skin regenerating effects of ATO-ALL, scratch wound healing assay, bromodeoxyuridine (BrdU) assay, and propidum iodide (PI) assay were performed using cultured HaCaT cell line. Result : Scratch wound healing assay showed that ATO-ALL was able to enhance the gap filling activity more than 2-fold at 7 ppm concentration compared with control group. BrdU assay demonstrated that ATO-ALL treatment increased the de novo cell proliferation in a dose-dependent manner. Finally, PI assay indicated that the cell cycle of HaCaT cells was modulated by ATO-ALL treatment. Conclusions : These results suggested that ATO-ALL may have skin regenerating effects by increasing cell proliferation via cell cycle regulation. Taken together, ATO-ALL is supposed to have a potential on regeneration of damaged skin or functional disease including atopic dermatitis.
Keywords
ATO-ALL; Skin; keratinocyte; wound healing; atopic dermatitis;
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