• 제목/요약/키워드: Skin contents

검색결과 637건 처리시간 0.026초

생강나무 추출물의 항산화 활성과 미백효과 (Antioxidant Activities and Whitening Effect from Lindera obtusiloba BL. Extract)

  • 방채영;원은경;박권우;이광원;정세영
    • 약학회지
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    • 제52권5호
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    • pp.355-360
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    • 2008
  • In this study we investigated antioxidant activity of against several free radicals and skin whitening effect of 70% ethanol extract (leaf extracts and branch/stem mixed) of Lindera obtusiloba BL. Antioxidant activity was assessed by DPPH, superoxide radical and hydroxyl radical assays. The Lindera obtusiloba BL. extract had antioxidant activity dose dependently with an ${IC}_{50}$ value of 243.14 and 181.10 ${\mu}g$/ml for DPPH, 165.77 and >1500 ${\mu}g$/ml for non-enzymatic system of superoxide radical assay, 35.47 and >100 ${\mu}g$/ml for enzymatic system of superoxide radical assay, 1.21 mg/ml for hydroxyl radical assay. In addition we tested tyrosinase inhibition activity and melanin contents on B16 melanoma F10. B16 melanoma cell was treated by such sample as 1, 5, 10 and 50 ${\mu}g$/ml for 72 hr and tyrosinase inhibition was tested. Melanogenesis was inhibited to 22% at the dose of 50 ${\mu}g$/ml and tyrosinase was inhibited to 45.2% at the same dose. In conclusion Lindera obtusiloba BL had potent antioxidant activity and inhibitory activity of tyrosinase and melanin formation. It could be developed as the health functional food and functional cosmetic resources.

수치에 의한 황금의 성분변화 (Change of Flavonoid Composition in Scutellaria baicalensis by Processing)

  • 김장희;양기숙;김태희
    • 약학회지
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    • 제43권1호
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    • pp.16-22
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    • 1999
  • Scutellaria baicalensis Georgi (Labiatae) has been extensively used in treatment of diarrhea, conjunctivitis, gastritis, enteritis and skin disease. As instructed in old description, the Scutellariae Radix should sometimes be processed before use. To 노디 light on the changes of chemical compositions by processing as well as processing method-activity relationships, Scutellariae Radix was heated at 16$0^{\circ}C$-20$0^{\circ}C$ for 30 min. in furnace or soaked with 20% EtOH (SPE) and boy's urine(SPU), respectively, which are one of processing methods and then heated at 17$0^{\circ}C$ for 30 min. To isolate the chemical components, Scutellariae Radix with/without processing were extracted with EtOH and EtOH extract was fractionated with ether, ethyl acetate and butanol to give respective fractions. Ether and EtOAc fractions obtained from the processed drug with urine (SPU) were subjected to chromatography to obtainsix components, oroxylin A, Wogonin, chrysin, baicalein, baicalein 7-O-$\beta$-D-glucopyranoside and $\beta$-sitosterol 3-O-$\beta$-D-glucopyranoside. All the isolated compounds were identified by means of physicochemical and spectroscopic methods (IR, $^1H-NMR,{\;}^13C-NMR$, Mass). By HPLC determination, the changes of the contents for each isolated components from SPE and SPU samples were observed. It was found that the content of nonglycosidic flavones such as oroxylin A, wogonin, chrysin and baicalein was increased markedly, whereas the content of baicalin and baicalein 7-O-$\beta$-D-glucopyranoside was significantly decreased in both samples as compared with those of Scutellariae Radix. When the sample was soaked with boy's urine, the total amount of nonglycosidic flavones was higher than that of processing with 20% EtOH.

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옻나무 추출물 첨가에 따른 영지버섯의 가나도마난디올 생합성 증대 및 멜라닌 생성 저해효과 (Enhancement of Ganodermanondiol and Anti-melanogenesis Effect of Ganoderma lucidum by Rhus verniciflua Extract Supplementation)

  • 정용운;김홍일;김종현;박영진
    • 대한화장품학회지
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    • 제43권4호
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    • pp.365-371
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    • 2017
  • 본 연구는 옻나무 추출물의 첨가가 영지버섯 미백활성 물질인 가나도마난디올의 생합성에 미치는 영향을 조사하기 위하여 수행하였다. 옻나무 열수 추출물을 1, 5, 10, 15%의 비율로 영지버섯 균사발효 시 첨가하고 발효 균사 추출물의 HPLC분석을 수행한 결과, 가나도마난디올 생합성이 첨가하지 않은 대조군에 비해 유의적으로 증가함을 확인하였다. 또한, 옻나무 열수 추출물이 첨가되어 발효된 영지 균사 추출물의 B16F10 세포주에 대한 멜라닌 생합성 저해능을 평가한 결과 첨가하지 않은 대조군에 비해 유의적으로 저해능이 증가함을 확인하였고, MTT분석을 통해 B16F10 세포주에 대한 세포생존율에도 영향을 미치지 않은 것을 확인하였다. 결과적으로 옻나무 추출물이 영지버섯균사의 미백활성 물질인 가나도마난디올 생합성을 촉진하여 향후 옻나무 추출물 첨가 영지버섯균사 발효 추출물이 효과적인 화장품 성분으로 사용 가능할 것으로 사료된다.

참당귀로부터 분리한 Demethylsuberosin의 멜라닌 생성 억제 효과 (Inhibitory Effects on Melanin Production of Demethylsuberosin Isolated from Angelica gigas Nakai)

  • 김유아;박성하;김보윤;김아현;박병준;김진준
    • 생약학회지
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    • 제45권3호
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    • pp.209-213
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    • 2014
  • The anti-melanogenic substance was isolated from the root of Angelica gigas Nakai by silica gel column chromatography, preparative HPLC and TLC. As a result of the structure analysis by mass, $^1H$-NMR, and $^{13}C$-NMR spectrometry, the compound was identified as demethylsuberosin. Demethylsuberosin reduced melanin contents of B16F1 melanoma cells in a dose-dependent manner and decreased to about 74% at a concentration $5{\mu}g/ml$. Demethylsuberosin inhibited the expression in microphthalmia associated transcription factor (MITF), tyrosinase, tyrosinase related protein-1 (TRP-1), and tyrosinase related protein-2 (TRP-2) in melanocytes. These results suggest that the whitening activity of demethylsuberosin may be due to the inhibition of the melanin synthesis by down-regulation of MITF, tyrosinase, TRP-1 and TRP-2 expression. Thus, our results provide evidence that demethylsuberosin might be useful as a potential skin-whitening agent.

사매 에탄올 추출물의 항산화 및 항염증 효과 (Antioxidative and Anti-inflammatory Effect of Ethanol Extract from Duchesnea chrysantha)

  • 이덕재;전인화;김현수;조일영;장선일
    • 동의생리병리학회지
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    • 제26권1호
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    • pp.59-66
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    • 2012
  • Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, including atopic dermatitis. The present study was undertaken to investigate the antioxidative and anti-inflammatory activities of the extract of Duchesnea chrysantha (DCE). DEC was prepared by extracting with 80% ethanol. Total flavonoids and polyphenols were measured by a colorimetric assay. The free radical scavenging activity of the extract was analyzed by the DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and Griess reagent assay. An oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The level of prostaglandin $E_2$ ($PGE_2$) was measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were measured by Western blot analysis. Total flavonoid and polyphenol contents of DCE were included $24.73{\pm}0.45$ and $178.77{\pm}2.65$, respectively. DCE significantly increased electron donating ability (DPPH), nitrite scavenging (NO) and ABTS reducing activity in dose dependant. We investigated the anti-inflammatory effects of DCE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. DCE significantly suppressed NO and prdstaglandin $E_2$ ($PGE_2$) in dose dependant. Furthermore, the levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with DCE in a dose dependent manner. These results suggest that DEC may has value as natural product with its high quality functional components, antioxidative and anti-inflammatory activities.

Ethyl oleate 처리에 의한 'Merlot' 포도 과피의 안토시아닌 함량과 해부학적 변화 (Anatomical Changes and Anthocyanin Contents of the Exocarp by Ethyl Oleate Treatment on 'Merlot' Grapes)

  • 이별하나;권용희;신경희;박희승
    • 원예과학기술지
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    • 제28권3호
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    • pp.370-373
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    • 2010
  • 'Merlot' 포도 품종의 수확전 ethyl oleate 처리는 외표피와 아표피의 두께를 감소시키며 착색을 현저히 향상시킨다. 무처리구의 과피 두께는 $126-189{\mu}m$인데 반하여 처리 과실의 과피두께는 $90-107{\mu}m$로 조사되었으며, 이러한 외표피와 아표피 층의 두께 감소는 처리 후 외표피와 아표피를 구성하는 세포의 빠른 노화에 의하여 세포가 죽거나 탈수가 나타나기 때문인 것으로 조사되었다. 처리 후에 빠르게 과피 표면의 왁스층이 녹은 듯한 형태를 보이며 이는 착색을 증진시키는 것으로 관찰되었으며, 전체적인 안토시아닌 함량 역시 증가하는 것으로 조사되었다. 반면에 각각의 안토시아닌 함량에 있어서는 메톡시기와 결합하거나 유기산과 결합한 안토시아닌이 증가한 반면 수산기와 결합한 안토시아닌은 감소하는 경향이었다.

히솝 추출물의 항산화 효과 및 멜라닌 생성 저해효과 (Antioxidant and Anti-Melanogenic Activities of Hyssopus officinalis Extracts)

  • 신서연;김하늘;강세원;조홍석;김은지;박선화;박경목
    • 대한화장품학회지
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    • 제42권2호
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    • pp.195-201
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    • 2016
  • 히솝(Hyssopus officinalis)은 통화식물목 꿀풀과의 여러해살이풀로 방부제, 기침, 거담제 등 일반적으로 방향족 허브와 약용식물로 사용되어왔다. 본 연구에서는 항산화 및 미백소재의 개발을 위해 히솝을 열수와 주정으로 추출하여 각 추출물의 in vitro 상에서의 항산화 효과 및 B16F10 melanoma 세포에서 멜라닌 생성 저해효과를 평가하였다. DPPH와 ABTS assay 시험법을 이용하여 항산화 효과를 측정한 결과, 히솝 추출물의 항산화 활성이 모두 농도 의존적으로 증가하였다. 또한 히솝 추출물은 tyrosianse 활성을 저해시켰으며, B16F10 세포에서 UVB로 증가된 활성산소와 ${\alpha}$-MSH로 유도된 멜라닌 또한 감소시키는 효과를 보였다. 멜라닌 생합성에 관여하는 유전자의 발현에 미치는 영향을 알아보기 위해 RT-PCR을 실시한 결과 히솝 추출물에 의해 MITF, tyrosinase, TRP-2의 발현이 감소되는 것을 확인할 수 있었다. 이러한 결과에 따라 히솝 추출물은 미백개선을 위한 화장품 소재로서 이용 가능성이 높을 것으로 사료된다.

여드름균(Cutibacterium acnes)에 대한 해조류 추출물의 항균효과 (In vitro Antimicrobial Activities of Edible Seaweeds Extracts Against Cutibacterium acnes)

  • 이명석;임미진;이정민;이대성;김영목;엄성환
    • 한국수산과학회지
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    • 제54권1호
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    • pp.111-117
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    • 2021
  • The study was aimed at developing an alternative therapeutic agent against acne vulgaris, one of the most common skin diseases, to meet the continuing demand for new therapies. Acne vulgaris is often associated with the acne-causing bacteria such as Cutibacterium acnes. To investigate the safety of agents against acne vulgaris, we evaluated the potential antibacterial activities of edible seaweeds against C. acnes in Korea. Forty-one edible seaweeds, including the brown, green, and red varieties, were selected for the antibacterial test. In comparison with other seaweeds, 70% ethanolic extracts of brown seaweeds, such as Cladophora wrightiana var. minor, Eisenia bicyclis, Ecklonia cava, Ishige foliacea, Ishige okamurae, Sargassum filicinum, and Sargassum miyabei Yendo, exhibited potential antibacterial activity against C. acnes with minimum inhibitory concentrations ranging between 64 and 128 ㎍/mL. To investigate the active anti-acne agents and to enhance our understanding of the antibacterial activities against C. acnes, further solvent-fractionation experiments are warranted. The findings imply that brown seaweeds can be a potential source of natural agents against acne vulgaris.

지누아리(Grateloupia filicina) 추출물의 항산화 및 항염능 (Antioxidant and Anti-inflammatory Activity of Grateloupia filicina extract)

  • 유미영;이상현
    • 융합정보논문지
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    • 제12권1호
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    • pp.119-126
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    • 2022
  • 본 연구에서는 최적의 조건에서 추출된 지누아리 추출물의 항산화능과 항염능을 확인하였다. 다양한 ethanol 수용액으로 지누아리의 유용물질을 추출하여 DPPH, ABTS, nitrite 라디컬 소거능을 측정하고, 폴리페놀 및 플라보노이드 함량을 분석하였다. 20% ethanol 수용액으로 추출한 경우에 라디컬 소거능이 가장 우수하였고, 폴리페놀과 플라보노이드의 함량 또한 가장 높았다. 이로부터 20% ethanol 수용액이 지누아리의 항산화 및 항염 물질을 효율적으로 추출하였음을 확인하였다. 한편, RAW 264.7 세포주를 이용하여 지누아리 추출물의 세포독성과 항염능을 확인하였다. 지누아리 추출물은 200 ㎍/mL의 농도에서 독성을 나타내지 않았고, 이 농도에서 50% 이상의 NO 생성 억제능을 나타냈다. 이러한 결과로 지누아리 추출물은 항산화 및 항염 특성을 지니는 기능성 화장품 원료로 사용 가능함을 확인하였다.

Optimization of the extraction process of high levels of chlorogenic acid and ginsenosides from short-term hydroponic-cultured ginseng and evaluation of the extract for the prevention of atopic dermatitis

  • Lee, Tae Kyung;Lee, Ji Yun;Cho, Yeon-Jin;Kim, Jong-Eun;Kim, Seo Yeong;Park, Jung Han Yoon;Yang, Hee;Lee, Ki Won
    • Journal of Ginseng Research
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    • 제46권3호
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    • pp.367-375
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    • 2022
  • Background: Short-term hydroponic-cultured ginseng (sHCG), which is 1-year-old ginseng seedlings cultivated for 4 weeks in a hydroponic system, is a functional food item with several biological effects. However, the optimal extraction conditions for sHCG, and the bioactivity of its extracts, have not been evaluated. Methods: Chlorogenic acid (CGA) and ginsenoside contents were evaluated in sHCG, white ginseng (WG), and red ginseng (RG) using high-performance liquid chromatography. Response surface methodology (RSM) was used to optimize the extraction conditions (temperature and ethanol concentration) to maximize the yield of dry matter, CGA, and four ginsenosides (Re, Rg1, Rb1, and Rd) from sHCG. The optimal extraction conditions were applied to pilot-scale production of sHCG extracts. The expression levels of tumor necrosis factor (TNF)-α/interferon (IFN)-γ-induced thymic and activation-regulated chemokines (TARC/CCL17) were measured after treatment with sHCG, WG, and RG extracts, and the effects of their bioactive compounds (CGA and four ginsenosides) on human skin keratinocytes (HaCaTs) were evaluated. Results: CGA and four ginsenosides, which are bioactive compounds of sHCG, significantly inhibited TNF-α/IFN-γ-induced TARC/CCL17 expression. The optimal sHCG extraction conditions predicted by the RSM models were 80 ℃ and 60% ethanol (v/v). The sHCG extracts produced at the pilot scale under optimal conditions greatly alleviated TNF-α/IFN-γ-induced TARC/CCL17 production compared with WG and RG extracts. Conclusions: Pesticide-free sHCG extracts, which contain high levels of CGA and the ginsenosides Re, Rg1, Rb1, and Rd as bioactive compounds, may have therapeutic potential for atopic diseases.