DOI QR코드

DOI QR Code

Antioxidant and Anti-inflammatory Activity of Grateloupia filicina extract

지누아리(Grateloupia filicina) 추출물의 항산화 및 항염능

  • Yu, Mi Young (Department of Biological Engineering, Konkuk University) ;
  • Lee, Sang Hyun (Department of Biological Engineering, Konkuk University)
  • 유미영 (건국대학교 생물공학과) ;
  • 이상현 (건국대학교 생물공학과)
  • Received : 2021.10.06
  • Accepted : 2022.01.20
  • Published : 2022.01.28

Abstract

In this study, the antioxidant ability, anti-inflammatory ability and whitening effect of the extracted Grateloupia filicina under optimal conditions were confirmed. The useful substances of Grateloupia filicina were extracted with various ethanol aqueous solutions to measure DPPH, ABTS, and nitrite radical elimination capabilities, and the polyphenol and flavonoid contents were analyzed. When extracted with a 20% ethanol aqueous solution, the radical elimination ability was the best, and the content of polyphenol and flavonoids was also the highest. From this, it was confirmed that the 20% ethanol aqueous solution efficiently extracted the antioxidant and anti-inflammatory substances of Grateloupia filicina. Meanwhile, the cytotoxicity and anti-inflammatory properties of the Grateloupia filicina extract were confirmed using RAW 264.7 cell lines. Grateloupia filicina extract did not show toxicity at a concentration of 200 ㎍/mL, and showed NO production inhibitory capacity of 50% or more at this concentration. As a result, it was confirmed that the Grateloupia filicina extract can be used as a functional cosmetic raw material having antioxidant and anti-inflammatory properties.

본 연구에서는 최적의 조건에서 추출된 지누아리 추출물의 항산화능과 항염능을 확인하였다. 다양한 ethanol 수용액으로 지누아리의 유용물질을 추출하여 DPPH, ABTS, nitrite 라디컬 소거능을 측정하고, 폴리페놀 및 플라보노이드 함량을 분석하였다. 20% ethanol 수용액으로 추출한 경우에 라디컬 소거능이 가장 우수하였고, 폴리페놀과 플라보노이드의 함량 또한 가장 높았다. 이로부터 20% ethanol 수용액이 지누아리의 항산화 및 항염 물질을 효율적으로 추출하였음을 확인하였다. 한편, RAW 264.7 세포주를 이용하여 지누아리 추출물의 세포독성과 항염능을 확인하였다. 지누아리 추출물은 200 ㎍/mL의 농도에서 독성을 나타내지 않았고, 이 농도에서 50% 이상의 NO 생성 억제능을 나타냈다. 이러한 결과로 지누아리 추출물은 항산화 및 항염 특성을 지니는 기능성 화장품 원료로 사용 가능함을 확인하였다.

Keywords

References

  1. J. W. Back & K. H. Lee. (2014). The Present of Convention on Biological Diversity Maritime Agenda. Korean journal of environmental biology, 32(4), 397-402. DOI : 10.11626/KJEB.2014.32.4.397
  2. J. S. Lee. (2008). Chemistry and Utilization of Algae. pp. 16-45. Hyoil, Seoul, Korea.
  3. S. J. Kim, K. S. Lee, S. H. Mo, J. B. Park, J. G. Oh, Y. J. Jeong, T. G. Kwon & T. G. Lee. (2013). Phenolic Contents and Antioxidant Activities of Six Edible Seaweeds. Journal of the Korea Academia-Industrial cooperation Society, 14(6), 3081-3088. DOI : 10.5762/KAIS.2013.14.6.3081
  4. C. Deville, M. Gharbi, G. Dandrifosse & O. Peulen. (2007). Study on the effects of laminarin, a polysaccharide from seaweed, on gut characteristics. Journal of the Science of Food and Agriculture, 87(9), 1717-1725. DOI : 10.1002/jsfa.2901
  5. Q. Wu, X. P. Fu, L. C. Sun, Q. Zhang, G. M. Liu, M. J. Cao & Q. F. Cai. (2015). Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R-phycoerythrin from red algae Bangia fusco-purpurea. International Journal of Food Science & Technology, 50(6), 1445-1451. DOI : 10.1111/ijfs.12775
  6. D. Lee, M. Nishizawa, Y. Shimizu & H. Saeki. (2017). Anti-inflammatory effects of dulse (Palmaria palmata) resulting from the simultaneous water-extraction of phycobiliproteins and chlorophyll a. Food Research International, 100, 514-521. DOI : 10.1016/j.foodres.2017.06.040
  7. S. Afreen & T. Fatma. (2018). Extraction, purification and characterization of phycoerythrin from Michrochaete and its biological activities. Biocatalysis and agricultural biotechnology, 13, 84-89. DOI : 10.1016/j.bcab.2017.11.012
  8. U. Panich, T. Onkoksoong, K. Kongtaphan, K. Kasetsinsombat, P. Akarasereenont & A. Wongkajornsilp. (2011). Inhibition of UVA-mediated melanogenesis by ascorbic acid through modulation of antioxidant defense and nitric oxide system. Archives of pharmacal research, 34(5), 811-820. DOI : 10.1007/s12272-011-0515-3
  9. E. Kohl, J. Steinbauer, M. Landthaler & R. M. Szeimies. (2011). Skin ageing. Journal of the European academy of dermatology and venereology, 25(8), 873-884. DOI : 10.1111/j.1468-3083.2010.03963.x
  10. Y. Athukorala, K. W. Lee, C. Song, C. B. Ahn, T. S. Shin, Y. J. Cha ... & Y. J. Jeon. (2003). Potential antioxidant activity of marine red alga Grateloupia filicina extracts. Journal of Food Lipids, 10(3), 251-265. DOI : 10.1111/j.1745-4522.2003.tb00019.x
  11. R. R. Sonani, N. K. Singh, J. Kumar, D. Thakar & D. Madamwar. (2014). Concurrent purification and antioxidant activity of phycobiliproteins from Lyngbya sp. A09DM: An antioxidant and anti-aging potential of phycoerythrin in Caenorhabditis elegans. Process Biochemistry, 49(10), 1757-1766. DOI : 10.1016/j.procbio.2014.06.022
  12. B. M. Kim, J. Y. Jeon, Y. B. Park & I. H. Jeong. (2006). Antioxidative Activity of Methanolic Extracts from Seaweeds. Journal of the Korean Society of Food Science and Nutrition, 35(8), 1097-1101. https://doi.org/10.3746/JKFN.2006.35.8.1097
  13. M. R. Jo, D. J. Lee & S. G. Yu. (2012). Radical Scavenging Activity of Ethanol Extracts and Solvent Partitioned Fractions from Various Red Seaweeds. Ocean and Polar Research, 34(4), 445-451. DOI : 10.4217/OPR.2012.34.4.445
  14. S. M. Ahn, Y. K. Hong, G. S. Kwon & H. Y. Sohn. (2011). Evaluation of antioxidant and nitrite scavenging activity of seaweed extracts. Journal of Life Science, 21(4), 576-583. DOI : 10.5352/JLS.2011.21.4.576
  15. C. S. Kwak, S. A. Kim & M. S. Lee. (2005). The Correlation of Antioxidative Effects of 5 Korean Common Edible Seaweeds and Total Polyphenol Content. Journal of the Korean Society of Food Science and Nutrition, 34(8), 1143-1150. DOI : 10.3746/jkfn.2005.34.8.1143
  16. C. Park & H. Yoon. (2019). Anti-Inflammatory and Antioxidative Effects of Gracilaria textorii Ethanol Extract in LPS-PG-Stimulated Human Gingival Fibroblast-1 Cells. Journal of The Korean Society of Integrative Medicine, 7(4), 61-69. DOI : 10.15268/ksim.2019.7.4.061
  17. D. Salvemini, Z. Q. Wang, P. S. Wyatt, D. M. Bourdon, M. H. Marino, P. T. Manning & M. G. Currie. (1996). Nitric oxide: a key mediator in the early and late phase of carrageenan-induced rat paw inflammation. British journal of pharmacology, 118(4), 829-838. DOI : 10.1111/j.1476-5381.1996.tb15475.x
  18. A. S. Michel, M. M. Mestdagh & M. A. V. Axelos. (1997). Physico-chemical properties of carrageenan gels in presence of various cations. International Journal of Biological Macromolecules, 21(1-2), 195-200. DOI : 10.1016/S0141-8130(97)00061-5