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Antioxidative and Anti-inflammatory Effect of Ethanol Extract from Duchesnea chrysantha  

Lee, Deok-Jae (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
Jeon, In-Hwa (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
Kim, Hyeon-Soo (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
Cho, Il-Young (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
Jang, Seon-Il (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.26, no.1, 2012 , pp. 59-66 More about this Journal
Abstract
Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, including atopic dermatitis. The present study was undertaken to investigate the antioxidative and anti-inflammatory activities of the extract of Duchesnea chrysantha (DCE). DEC was prepared by extracting with 80% ethanol. Total flavonoids and polyphenols were measured by a colorimetric assay. The free radical scavenging activity of the extract was analyzed by the DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and Griess reagent assay. An oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The level of prostaglandin $E_2$ ($PGE_2$) was measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were measured by Western blot analysis. Total flavonoid and polyphenol contents of DCE were included $24.73{\pm}0.45$ and $178.77{\pm}2.65$, respectively. DCE significantly increased electron donating ability (DPPH), nitrite scavenging (NO) and ABTS reducing activity in dose dependant. We investigated the anti-inflammatory effects of DCE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. DCE significantly suppressed NO and prdstaglandin $E_2$ ($PGE_2$) in dose dependant. Furthermore, the levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with DCE in a dose dependent manner. These results suggest that DEC may has value as natural product with its high quality functional components, antioxidative and anti-inflammatory activities.
Keywords
Duchesnea chrysantha; ethanol extract; antioxidant; anti-inflammation;
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