• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.5
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• pp.887-893
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• 2011

The purpose of this study was to investigate the wound healing effects of Danggwieumja (DG), which is commonly used for skin inflammation, skin wound, skin pruritus, and chronic hives etc. The 1.5 cm ${\times}$ 1.5 cm full-thickness skin wound was induced to two groups, DG (n=16) and Saline (n=16) group. The DG extract and Saline were orally administrated daily for 15 days after skin wound induction. Then, the body weight of rats and the congestion indices were daily measured for 15 days after skin wound induction. The wound contractions and epithelizations were also measured. The wound contractions were daily measured for 15 days after wound induction and wound epithelizations were measured for 8 days from day 7 after wound induction. For evaluating angiogenesis, the immunoreactivities of vWF and VEGF protein were measured immunohistochemistrically on day 15. In results, although the percentage increases in mean body weight of rats in the DG and Saline groups hve no significant differences, DG extract decreased the time of wound healing and congestion around wound, and improved wound contraction and epithelization. The contraction percentage of DG group was significantly increased on day 5 (P<0.05) and day 7 (P<0.01) than that of Saline group. DG group showed significant increase of wound epithelization on day 7 (P<0.05) as compared to Saline group. Moreover, DG extract reduced the inflammation of skin dermis and promoted the growth of vascular vessels of dermis by accelerating vascular endothelial growth factor (VEGF) protein. These results suggest that DG has the beneficial effects on skin incision wound and can be the suitable wound healing agent for various surgical wounds.

• KSBB Journal
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• v.23 no.1
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• pp.8-17
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• 2008

There are many different approaches to healing of acute and chronic ulcer and large skin defect, such as burn. Currently available wound covers fall into two categories. Permanent covering, such as autografts, and temporary ones, such as allograft including de-epidermized cadaver skin, bioartificial skin, xenografts, and synthetic dressings. Autologous skin grafting in the form of split- or full-thickness skin is still the good standard. Following on from developments in the 1980s involving the use of cultured keratinocyte grafts in wound healing, the last decade has been great progress in the fabrication of composite bioartificial skin grafts. However, two bottleneck on producing cultured bioartificial skin, whether of the simple epithelial cell sheet type, or the more complex composite type, continue to be the generation of sufficient keratinocytes cheaply and quickly and develop biocompatible dermal scaffolds. This article covers the development, clinical application, and current research directions associated with bioartificial skin.

• Journal of Sasang Constitutional Medicine
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• v.24 no.4
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• pp.1-16
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• 2012

Objectives : We aimed to analyze the meanings of the energy and fluid metabolism in the Discourse on Viscera and Bowels of Donguisusebowon, and to find the clues for the explanation of the pathology and symptomatology of Taeeumin. Methods : The Discourse on Viscera and Bowels of Donguisusebowon was reviewed and examined for relevant information on the energy and fluid metabolism from the structural and the functional point of view respectively. And, based on the derived meanings of the energy and fluid metabolism, the pathology and symptomatology of Taeeumin were analyzed. Results and Conclusions : 1. The meanings of the energy and fluid metabolism can be explained by the different attributes of the energy and fluid produced from the esophagus and the small intestine, and the different function of exhaling-dispersing and inhaling-concentrating in the different tract of circulation such as Lung affiliation (esophagus, skin, ear and lung) and Liver affiliation (small intestine, flesh, nose and liver). 2. The Exterior disease of Taeeumin starts with the weakness of exhaling-dispersing function at the skin, and leads to the dysfunction of the esophagus and the lung sequently. The dysfunction of the lung aggravates that of the skin and the esophagus. 3. The Interior disease of Taeeumin begins with excess of the inhaling-concentrating functions at the flesh and the small intestine, and leads to the dysfunction of the lung, which induces the dysfunction of exhaling-concentration at the skin and esophagus. And, this disparities between exhaling-dispersing and inhaling-concentrating functions exasperate the problem at the flesh and the small intestine.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.5
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• pp.1099-1107
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• 2007

This study was investigated the anti-allergic effect of SJSBT on DNCB induced atopic dermatitis in NC/Nga mice. We summerized as the follow. SJSBT significantly decreased the clinical manifestations of atopic dermatitis including itching, dryness, edema, hemorrhage, and lichenification in dose dependent manner. SJSBT markedly suppressed invasion and edema of leukocytes and mast cell in dorsal skin and ear tissue. SJSBT significantly reduced the number of CD11b+/Gr-1 cell compared with positive control, but it had no affect the number of CD3+ and CCR3+/CD3+ cell. SJSBT markedly suppressed the expression of cytokine and chemokine such as IL-6, $TNF-{\alpha}$, eotaxin and CCR3 compared with positive control group. SJSBT significantly decreased the invasion of CD4+ and CCR3+ cell in ear and dorsal skin tissue compared with positive control group by using immunohistochemical staining. Taken together, these findings suggested that SJSBT has an anti-allergic activity and this might be useful for the clinical application to treat allergic diseases such as atopic dermatitis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.2
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• pp.392-398
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• 2007

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by inflammatory cell infiltration in the skin. This study was performed to assess the therapeutic effects of YHYBT on the DNCB-induced dermatitis in NC/Nga mice, characterized by the onset of AD along with an increase the number of Th2 cells and dysregulation of inflammaroty mediators including cytokines and chemokines. YHYBT administration significantly reduced clinical dermatitis severity including pruritus, edema, eczematous and erythema. Histological findings indicated that the thickening of epidermis/dermis and dermal infiltration of inflammatory cells including mast cells were dramatically reduced. The suppression of dermatitis by YHYBT was accompanied by a decrease in the total number of immune cells in drained lymph node (DLN) and skin. Especially CD3+, CD4+ and CD3+CD69+ T cells in PBMC and DNL were greatly reduced. The level of IL-4 in CD3/CD28 activated splenocyte was downregulated, whereas that of IFN-'처리불가‘ was increased. Furthermore, the expression of eotaxin2 and CCR3 in skin were significanlty decreased. These data suggest that YHTBT may be effective therapeutic agents for the treatment of AD.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.5
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• pp.1343-1346
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• 2003

Monitoring the skin temperature changes of the palm during video endoscopic thoracic sympathicotomy has been used as a measure of the success of the surgery for palmar hyperhidrosis. So we measured palmar skin temperature on both sides after administration of herbal medicine to learn whether we can use it as a indicator of herbal medicine effect, and to see it's mechanism. Seven patients (15 to 19 years old) with palmar hyperhidrosis underwent administration of herbal medicine. The palmar skin temperature was measured by comparing the changes of skin temperature before and after administration of herbal medicine for palmar hyperhidrosis. After the administration, temperature of the left palm was elevated(before. 26.16±2.13℃ vs. after. 30.62±1.84℃, p<0.05), and temperature of the right palm was also elevated(before. 26.14±2.28℃ vs. after. 30.91 ±1.67℃, p<0.05). These results suggest that the administration of herbal medicine is not only the symptoms of hyperhidrosis abolished but also the skin temperature of palm is elevated.

• Korean Journal of Veterinary Pathology
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• v.3 no.1
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• pp.45-50
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• 1999

Canine dermatosis associated with zinc deficiency naturally occurred in three districts of Kyungpook. A diagnosis was made by clinical findings, clinico-pathological examinations, skin biopsy, and therapeutic tests. The result was diagnosed as a zinc-deficient dermatosis. The disease occurred in rapidly growing Tosa-dog hybrids 5 to 7 months old. Skin lesions included erytherma, alopecia, crusting, scaling around the mouth, eyes, legs and tail. Clinico-pathological findings were decreased serum zinc levels, slightly increased white blood cells, and secondary Staphylococcus intermedius infection of the crusts. The skin biopsies for histopathological examination revealed marked parakeratotic hyperkeratosis. The patients with skin lesions were successfully treated by zinc methonine(Zinpro) medication In conclusion, serum zinc level and skin biopsy are very helpful in diagnosis of canine zinc-deficient dermatosis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.6
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• pp.1062-1067
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• 2010

The purpose of this study was to estimate skin care effect of Miso Facial Rejuvenation Acupuncture with the facial skin photographing system and ultrasonography. 4 Patients who made first visits to Daegu Oriental Hospital of Daegu Hanny University from August 7, 2009 to August 12, 2009 were each given five-times treatments of Miso Facial Rejuvenation Acupunture. The facial skin photography of each participant was checked two times-pretreatment, posttreatment-using the facial skin photographing system and ultrasonography. The squares and counts of 2parts of facial winkles and thickness of masseter muscle were measured for each one. The squares and counts of facial wrinkles were decreased. The thickness of masseter muscle were decreased. On this study, we could evaluate the wrinkle treatment effects and reduction of masseter muscle of Miso Facial Rejuvenation Acupuncture by an objective method. Miso Facial Rejuvenation Acupuncture has notable effect in wrinkle treatment and reduction of masseter muscle. As though we had not wide experience in this treatment, more research is needed.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.30 no.4
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• pp.308-315
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• 2004

Aim: The aim of this study was to investigate the mechanism of promoted skin wound healing in skin defects with primary cultured oral mucosal keratinocytes. Materials and methods: Thirty adult female nude mice weighing $20{\pm}2g$ were used for the experiment. Primary cultured and suspended oral mucosal keratinocytes, labeled with BrdU, were scattered onto $1.5cm{\times}1.5cm$ sized full thickness skin defects in the experimental group(N=15), and no grafts were placed the control group(N=15). They were sacrificed at 3 days, 1 week and 2 weeks after the treatment respectively. Histological examination of each wounds were performed to review the healing progress on measuring the length from the wound margin to regenerating epithelial front. The role of keratinocytes were assessed by double immunohistochemical staining with Anti-BrdU and Anti-cytokeratin AE1/3. Results: In the experimental group the wound was completely covered with regenerating epithelia in 2 weeks, but partially regenerated in the control group. The immunohistochemical studies unexpectedly reveal that most of regenerating epithelial cells were induced from marginal epithelium of the margin, not from the scattered keratinocytes. Conclusion: We could successfully confirm that graft of primary cultured oral mucosal keratinocytes promotes the regeneration of skin defects.

• Journal of fish pathology
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• v.30 no.2
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• pp.97-105
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• 2017

Parasitic leeches could directly (through causing poor growth, anemia and wound in the fish) and indirectly (by predisposition of the fish to secondary bacterial and fungal infections) affects their hosts. In the present study, fishes that were attacked by leeches in natural and experimental environment were studied. Pathologic samples were obtained from damages at the site of leech bite, as well as kidney and liver of the fish. Histopathological examination revealed numerous lesions at the site of leech bite including tissue demolition, detachment at the site of leech bite in the epidermis of epithelial tissue in the skin, destructed nucleus in epithelial cells of the skin plus necrosis in the damaged skin and weak inflammatory penetration to acute necrotic damages along with piercing dermis layer. Pathologic lesions in the kidney included some changes such as proliferation by increasing glomerular cells and membrane cells in capillary vein of the kidney, blood cell necrosis in kidney with infiltration of white blood cells mainly mononuclear and less polymorphonuclear which are the symptoms of anemia due to blood feeding and sucking by leeches. There was also a chronic kidney infection probably originated from another part of body such as skin. Moreover, leeches caused hemorrhagic anemia due to blood consumption of the hosts, which led to observation of immature red blood cells. Also results showed that diseases induced by leeched in fish could be acute or chronic, which depends on size of fish, species of leech and severity of infection.