Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Danggwieumja on the Healing of Full-Thickness Skin Injury in Rat

  • Kim, Bum-Hoi (Department of Anatomy, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University)
  • Received : 2011.08.04
  • Accepted : 2011.09.10
  • Published : 2011.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate the wound healing effects of Danggwieumja (DG), which is commonly used for skin inflammation, skin wound, skin pruritus, and chronic hives etc. The 1.5 cm ${\times}$ 1.5 cm full-thickness skin wound was induced to two groups, DG (n=16) and Saline (n=16) group. The DG extract and Saline were orally administrated daily for 15 days after skin wound induction. Then, the body weight of rats and the congestion indices were daily measured for 15 days after skin wound induction. The wound contractions and epithelizations were also measured. The wound contractions were daily measured for 15 days after wound induction and wound epithelizations were measured for 8 days from day 7 after wound induction. For evaluating angiogenesis, the immunoreactivities of vWF and VEGF protein were measured immunohistochemistrically on day 15. In results, although the percentage increases in mean body weight of rats in the DG and Saline groups hve no significant differences, DG extract decreased the time of wound healing and congestion around wound, and improved wound contraction and epithelization. The contraction percentage of DG group was significantly increased on day 5 (P<0.05) and day 7 (P<0.01) than that of Saline group. DG group showed significant increase of wound epithelization on day 7 (P<0.05) as compared to Saline group. Moreover, DG extract reduced the inflammation of skin dermis and promoted the growth of vascular vessels of dermis by accelerating vascular endothelial growth factor (VEGF) protein. These results suggest that DG has the beneficial effects on skin incision wound and can be the suitable wound healing agent for various surgical wounds.

Keywords

References

  1. Han, D., Kim, H.Y., Lee, H.J., Shim, I., Hahm, D.H. Wound healing activity of gamma-aminobutyric Acid (GABA) in rats. J Microbiol Biotechnol. 17(10):1661-1669, 2007.
  2. Clark, R.A. Cutaneous tissue repair: basic biologic considerations. I. J Am Acad Dermatol. 13(5):701-725, 1985. https://doi.org/10.1016/S0190-9622(85)70213-7
  3. Ermertcan, A.T., Inan, S., Ozturkcan, S., Bilac, C., Cilaker, S. Comparison of the effects of collagenase and extract of Centella asiatica in an experimental model of wound healing: an immunohistochemical and histopathological study. Wound Repair Regen. 16(5):674-681, 2008. https://doi.org/10.1111/j.1524-475X.2008.00417.x
  4. Luo, J.D., Chen, A.F. Nitric oxide: a newly discovered function on wound healing. Acta Pharmacol Sin. 26(3):259-264, 2005. https://doi.org/10.1111/j.1745-7254.2005.00058.x
  5. Singer, A.J., Clark, R.A. Cutaneous wound healing. N Engl J Med. 341(10):738-746, 1999. https://doi.org/10.1056/NEJM199909023411006
  6. Dubay, D.A., Franz, M.G. Acute wound healing: the biology of acute wound failure. Surg Clin North Am. 83(3):463-481, 2003. https://doi.org/10.1016/S0039-6109(02)00196-2
  7. Breitenbach, K.L., Bergera, J.J. Principles and techniques of primary wound closure. Prim Care. 13(3):411-431, 1986.
  8. Yildiz, S., Cimsit, M., Ilgezdi, S., Uzun, G., Gumus, T., Qyrdedi, T., Dalci, D. Hyperbaric oxygen therapy used to treat radiation injury: two case reports. Ostomy Wound Manage. 52(5):14-20, 2006.
  9. Thompson, C., Fuhrman, M.P. Nutrients and wound healing: still searching for the magic bullet. Nutr Clin Pract. 20(3):331-347, 2005. https://doi.org/10.1177/0115426505020003331
  10. Folkman, J., Shing, Y. Angiogenesis. J Biol Chem. 267(16):10931-10934, 1992.
  11. Risau, W. Mechanisms of angiogenesis. Nature. 386(6626):671-674, 1997. https://doi.org/10.1038/386671a0
  12. Li, J., Zhang, Y.P., Kirsner, R.S. Angiogenesis in wound repair: angiogenic growth factors and the extracellular matrix. Microsc Res Tech. 60(1):107-114, 2003. https://doi.org/10.1002/jemt.10249
  13. Tonnesen, M.G., Feng, X., Clark, R.A. Angiogenesis in wound healing. J Investig Dermatol Symp Proc. 5(1):40-46, 2000. https://doi.org/10.1046/j.1087-0024.2000.00014.x
  14. Ferrara, N. Role of vascular endothelial growth factor in the regulation of angiogenesis. Kidney Int. 56(3):794-814, 1999. https://doi.org/10.1046/j.1523-1755.1999.00610.x
  15. Bitto, A., Minutoli, L., Altavilla, D., Polito, F., Fiumara, T., Marini, H., Galeano, M., Calò, M., Lo Cascio P., Bonaiuto, M., Migliorato, A., Caputi, A.P., Squadrito, F. Simvastatin enhances VEGF production and ameliorates impaired wound healing in experimental diabetes. Pharmacol Res. 57(2):159-169, 2008. https://doi.org/10.1016/j.phrs.2008.01.005
  16. Roy, H., Bhardwaj, S., Ylä-Herttuala, S. Biology of vascular endothelial growth factors. FEBS Lett. 580(12):2879-2887, 2006. https://doi.org/10.1016/j.febslet.2006.03.087
  17. Nissen, N.N., Polverini, P.J., Koch, A.E., Volin, M.V., Gamelli, R.L., DiPietro, L.A. Vascular endothelial growth factor mediates angiogenic activity during the proliferative phase of wound healing. Am J Pathol. 152(6):1445-1452, 1998.
  18. Im, G.M., Jeong, H.W., Kim, H.S., Jeong, W.Y. Oriental Medical Approach on the Allergic Disease. Korean J. Oriental Physiology & Pathology. 15(5):831-839, 2002.
  19. Han, K.H., Park, E.J., Lee, H.J. A Comparative Study of Regulatory Effect of Atopic Allergic Reaction by Prescriptions (A, B). J Korean Oriental Pediatrics 19(2):13-30, 2005.
  20. Song, S.P., Son, D.B., Hwang, C.H., Song, S.H., Hwang, C.Y. Effects of Danggwieumja Administration along with Samhwangseje-gamibang on NC/Nga Atopic Mice. Korean J. Oriental Physiology & Pathology. 21(5):1210-1218, 2007.
  21. Judith, R., Nithya, M., Rose, C., Mandal, A.B. Application of a PDGF-containing novel gel for cutaneous wound healing. Life Sci. 87(1-2):1-8, 2010. https://doi.org/10.1016/j.lfs.2010.05.003
  22. Choi, K.M., Lee, C.W., Lee, M.Y. Effect of Ore Minerals on the Healing of Full-Thickness Skin Injury Model of Rat. Jounal of the Environmental Sciences. 17(7):809-816, 2008. https://doi.org/10.5322/JES.2008.17.7.809
  23. Jung, S.I., Kang, S.S., Cho, S.K., Choi, S.H. Effect of propolis on healing of full-thickness skin wound in rabbits. Korea J. Vet. Clin. Med. 17(1):62-29, 2000.
  24. Diegelmann, R.F., Evans, M.C. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 9: 283-289, 2004. https://doi.org/10.2741/1184
  25. Jacobs, S., Simhaee, D.A., Marsano, A., Fomovsky, G.M., Niedt, G., Wu, JK. Efficacy and mechanisms of vacuum-assisted closure (VAC) therapy in promoting wound healing: a rodent model. J Plast Reconstr Aesthet Surg. 62(10):1331-1338, 2009. https://doi.org/10.1016/j.bjps.2008.03.024
  26. Sugihara, A., Sugiura, K., Morita, H., Ninagawa, T., Tubouchi, K., Tobe, R., Izumiya, M., Horio, T., Abraham, N.G., Ikehara, S. Promotive effects of a silk film on epidermal recovery from full-thickness skin wounds. Proc Soc Exp Biol Med. 225(1):58-64, 2000. https://doi.org/10.1046/j.1525-1373.2000.22507.x
  27. Werner, S., Grose, R. Regulation of wound healing by growth factors and cytokines. Physiol Rev. 83(3):835-970, 2003. https://doi.org/10.1152/physrev.2003.83.3.835
  28. Diegelmann, R.F., Evans, M.C. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 9: 283-289, 2004. https://doi.org/10.2741/1184
  29. Hayashi, T., Ishida, Y., Kimura, A., Takayasu, T., Eisenmenger, W., Kondo, T. Forensic application of VEGF expression to skin wound age determination. Int J Legal Med. 118(6):320-325, 2004. https://doi.org/10.1007/s00414-004-0468-x
  30. Takamiya, M., Saigusa, K., Aoki, Y. Immunohistochemical study of basic fibroblast growth factor and vascular endothelial growth factor expression for age determination of cutaneous wounds. Am J Forensic Med Pathol. 23(3):264-267, 2002. https://doi.org/10.1097/00000433-200209000-00012