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Suppression of DNCB-induced Dermatitis in NC/Nga Murine Model by Yanghyulyoonbu-tang  

Park, Byung-Jun (Department of Pathology, Daejeon University)
Choi, Jeong-June (Department of Pathology, Daejeon University)
Kim, Soo-Myung (Department of Anatomy Daejeon University)
Lee, Yong-Koo (Department of Internal medicine, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.2, 2007 , pp. 392-398 More about this Journal
Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by inflammatory cell infiltration in the skin. This study was performed to assess the therapeutic effects of YHYBT on the DNCB-induced dermatitis in NC/Nga mice, characterized by the onset of AD along with an increase the number of Th2 cells and dysregulation of inflammaroty mediators including cytokines and chemokines. YHYBT administration significantly reduced clinical dermatitis severity including pruritus, edema, eczematous and erythema. Histological findings indicated that the thickening of epidermis/dermis and dermal infiltration of inflammatory cells including mast cells were dramatically reduced. The suppression of dermatitis by YHYBT was accompanied by a decrease in the total number of immune cells in drained lymph node (DLN) and skin. Especially CD3+, CD4+ and CD3+CD69+ T cells in PBMC and DNL were greatly reduced. The level of IL-4 in CD3/CD28 activated splenocyte was downregulated, whereas that of IFN-'처리불가‘ was increased. Furthermore, the expression of eotaxin2 and CCR3 in skin were significanlty decreased. These data suggest that YHTBT may be effective therapeutic agents for the treatment of AD.
Keywords
Yanghyulyoonbu-tang(YHYBT); atopic dermatitis; NC/Nga mice; immune modulation; T cell; cytokines;
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1 Spergel, J.M., Mizoguchi, E., Oettgen, H., Bhan, A.K. and Geha, R.S. Roles of TH1 and TH2 cytokines in a murine model of allergic dermatitis. J Clin Invest 103(8):1103-1111, 1999   DOI   ScienceOn
2 Lester, M.R., Hofer, M.F., Gately, M., Trumble, A. and Leung, D.Y. Down-regulating effects of IL-4 and IL-10 on the IFN-gamma response in atopic dermatitis. J Immunol 154(11):6174-6181, 1995
3 Robert, C. and Kupper, T.S. Inflammatory skin diseases, T cells, and immune surveillance. N Engl J Med 341(24):1817-1828, 1999   DOI   ScienceOn
4 van der Heijden, F.L., Wierenga, E.A., Bos, J.D. and Kapsenberg, M.L. High frequency of IL-4-producing CD4+ allergen-specific T lymphocytes in atopic dermatitis lesional skin. J Invest Dermatol 97(3):389-394, 1991   DOI   ScienceOn
5 Heishi, M., Imai, Y., Katayama, H., Hashida, R., Ito, M., Shinagawa, A. and Sugita, Y. Gene expression analysis of atopic dermatitis-like skin lesions induced in NC/Nga mice by mite antigen stimulation under specific pathogen-free conditions. Int Arch Allergy Immunol 132(4):355-363, 2003   DOI   ScienceOn
6 Daniels, J. and Harper, J. The epidemiology of atopic dermatitis. Hosp Med 63(11):649-652, 2002   DOI
7 Chen, L., Martinez, O., Overbergh, L., Mathieu, C., Prabhakar, B.S. and Chan, L.S. Early up-regulation of Th2 cytokines and late surge of Th1 cytokines in an atopic dermatitis model. Clin Exp Immunol 138(3):375-387, 2004   DOI
8 이경미. 養血潤膚湯의 免疫 調節作用을 통한 항알러지 效能. 대전대학교대학원, 대전, 2007
9 Sampson, H.A. Atopic dermatitis. Ann Allergy 69(6):469-479, 1992
10 Stevens, S.R., Hanifin, J.M., Hamilton, T., Tofte, S.J. and Cooper, K.D. Long-term effectiveness and safety of recombinant human interferon gamma therapy for atopic dermatitis despite unchanged serum IgE levels. Arch Dermatol 134(7):799-804, 1998   DOI   ScienceOn
11 Yawalkar, N., Uguccioni, M., Scharer, J., Braunwalder, J., Karlen, S., Dewald, B., Braathen, L.R. and Baggiolini, M. Enhanced expression of eotaxin and CCR3 in atopic dermatitis. J Invest Dermatol 113(1):43-48, 1999   DOI   ScienceOn
12 Leung, D.Y. Atopic dermatitis: immunobiology and treatment with immune modulators. Clin Exp Immunol 107 Suppl 1: 25-30, 1997
13 Clevers, H., Alarcon, B., Wileman, T. and Terhorst, C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annu Rev Immunol 6: 629-662, 1988   DOI   ScienceOn
14 Leung, D.Y. and Soter, N.A. Cellular and immunologic mechanisms in atopic dermatitis. J Am Acad Dermatol 44(1 Suppl):S1-S12, 2001   DOI   ScienceOn
15 Bieber, T., Rieger, A., Stingl, G., Sander, E., Wanek, P. and Strobel, I. CD69, an early activation antigen on lymphocytes, is constitutively expressed by human epidermal Langerhans cells. J Invest Dermatol 98(5):771-776, 1992   DOI   ScienceOn
16 Cooper, K.D. Atopic dermatitis: recent trends in pathogenesis and therapy. J Invest Dermatol 102(1):128-137, 1994   DOI   ScienceOn
17 Renz, H., Jujo, K., Bradley, K.L., Domenico, J., Gelfand, E.W. and Leung, D.Y. Enhanced IL-4 production and IL-4 receptor expression in atopic dermatitis and their modulation by interferon-gamma. J Invest Dermatol 99(4):403-408, 1992   DOI   ScienceOn
18 Ma, W., Bryce, P.J., Humbles, A.A., Laouini, D., Yalcindag, A., Alenius, H., Friend, D.S., Oettgen, H.C., Gerard, C. and Geha, R.S. CCR3 is essential for skin eosinophilia and airway hyperresponsiveness in a murine model of allergic skin inflammation. J Clin Invest 109(5):621-628, 2002   DOI
19 Vestergaard, C., Yoneyama, H., Murai, M., Nakamura, K., Tamaki, K., Terashima, Y., Imai, T., Yoshie, O., Irimura, T., Mizutani, H. and Matsushima, K. Overproduction of Th2-specific chemokines in NC/Nga mice exhibiting atopic dermatitis-like lesions. J Clin Invest 104(8):1097-1105, 1999   DOI   ScienceOn
20 Schneider, L.C., Baz, Z., Zarcone, C. and Zurakowski, D. Long-term therapy with recombinant interferon-gamma (rIFN-gamma) for atopic dermatitis. Ann Allergy Asthma Immunol 80(3):263-268, 1998   DOI
21 Vestergaard, C., Yoneyama, H. and Matsushima, K. The NC/Nga mouse: a model for atopic dermatitis. Mol Med Today 6(5):209-210, 2000   DOI   ScienceOn