Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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THE INDUCTIVE CAPACITY OF PRIMARY CULTURED ORAL MUCOSAL KERATINOCYTES IN SKIN WOUND HEALING OF ATHYMIC NUDE MICE

배양된 구강점막 각화상피세포가 누드마우스 피부 창상 치유에 미치는 효과

  • Kim, Hyun-Sil (Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry, Brain Korea 21 project for Medical Science) ;
  • Kim, Nam-Hee (Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry, Brain Korea 21 project for Medical Science) ;
  • Kim, Jin (Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry, Brain Korea 21 project for Medical Science) ;
  • Cha, In-Ho (Department of Oral and Maxillofacial Surgery, Oral Cancer Research Institute, Yonsei University College of Dentistry, Brain Korea 21 project for Medical Science)
  • 김현실 (연세대학교 치과대학 구강병리학교실, 구강종양연구소, 두뇌한국 21 의과학사업단) ;
  • 김남희 (연세대학교 치과대학 구강병리학교실, 구강종양연구소, 두뇌한국 21 의과학사업단) ;
  • 김진 (연세대학교 치과대학 구강병리학교실, 구강종양연구소, 두뇌한국 21 의과학사업단) ;
  • 차인호 (연세대학교 치과대학 구강악안면외과학교실, 구강종양연구소, 두뇌한국 21 의과학사업단)
  • Published : 2004.08.31
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Abstract

Aim: The aim of this study was to investigate the mechanism of promoted skin wound healing in skin defects with primary cultured oral mucosal keratinocytes. Materials and methods: Thirty adult female nude mice weighing $20{\pm}2g$ were used for the experiment. Primary cultured and suspended oral mucosal keratinocytes, labeled with BrdU, were scattered onto $1.5cm{\times}1.5cm$ sized full thickness skin defects in the experimental group(N=15), and no grafts were placed the control group(N=15). They were sacrificed at 3 days, 1 week and 2 weeks after the treatment respectively. Histological examination of each wounds were performed to review the healing progress on measuring the length from the wound margin to regenerating epithelial front. The role of keratinocytes were assessed by double immunohistochemical staining with Anti-BrdU and Anti-cytokeratin AE1/3. Results: In the experimental group the wound was completely covered with regenerating epithelia in 2 weeks, but partially regenerated in the control group. The immunohistochemical studies unexpectedly reveal that most of regenerating epithelial cells were induced from marginal epithelium of the margin, not from the scattered keratinocytes. Conclusion: We could successfully confirm that graft of primary cultured oral mucosal keratinocytes promotes the regeneration of skin defects.

Keywords

References

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