• Bulletin of the Korean Chemical Society
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• 제34권2호
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• pp.645-648
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• 2013

• 한국미생물·생명공학회지
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• 제23권2호
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• pp.150-155
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• 1995

Carboxypeptidase Z(CPZ) cDNA of Absidia zychae was experssed in Saccharomyces cerevisiae. The expressed CPZ(YCPZ) was secreted about 30 mg/l into the medium and has a little higher molecular weight than the wild type CPZ in SDS-PAGE. By the result of N-terminal amino acid sequencing, YCPZ has additional 15 amino acids residues in N-terminus of CPZ. But YCPZ shows no difference with CPZ in enzyme activity and substrate specificity. For the identification of processing mechanism of YCPZ, 36-Arg was changed to 36-Thr by site specific mutagenesis. Mutant YCPZ does not processed at 36-Thr. It was, therefore, concluded that the YCPZ was processed by KEX2. According to endo F treatment, high amount of carbohydrate was N-glycosylated in YCPZ.

• 약학회지
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• 제47권5호
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• pp.266-270
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• 2003

We evaluated four commercially available methamphetamine immunoassays for their relative cross-reactivities of amphetamine analogues in human urine: Abbott TDx, Vitalab Selectra and on-site test kits (Accusign MET, SD bioline MET). High cross-reactivities were shown at designer's drugs such as methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA) and methylenedioxyethylamphetamine (MDEA) in all of the tested immunoassays. Methoxyphenamine, fenfluramine and phentermine were positive in TDx and Selectra, but were not positive in on-site test kits. Pseudoephedrine, norpseudoephedrine, ephedrine, norephedrine, MDMA, MDA, fenfluramine and phentermine were detected by gas chromatography/mass spectrometry(GC/MS) in false positive urines. Since the overall specificity of any of the devices was not 100％, we found it is important to confirm any positive screening test result, so we developed simultaneous determination of amphetamine analogues in urines. After alkalinization of the urine samples with 6-N NaOH, the analytes were extracted using ethyl acetate, derivatized with pentafluoropropyl anhydride (PFPA) prior at GC/MS analysis.

• BMB Reports
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• 제51권12호
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• pp.609-610
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• 2018

Glycosylation is one form of protein modification and plays a key role in protein stability, function, signaling regulation and even cancer. NleB and SseK are bacterial effector proteins and possess glycosyltransferase activity, even though they have different substrate preferences. NleB/SseKs transfer the GlcNAc sugar to an arginine residue of host proteins, leading to reduced $NF-{\kappa}B-dependent$ responses. By combining X-ray crystallography, NMR, molecular dynamics, enzyme kinetic assays and in vivo experiments, we demonstrated that a conserved HEN (His-Glu-Asn) motif in the active site plays a key role in enzyme catalysis and virulence. The lid-domain regulates the opening and closing of the active site and the HLH domain determines the substrate specificity. Our findings provide evidence for the enzymatic mechanism by which arginine can be glycosylated by SseK/NleB enzymes.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2021년도 가을 학술논문 발표대회
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• pp.40-41
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• 2021

Recently, particulate matter(PM) caused by internal factors such as industrialization and urbanization as well as external factors such as Asian dust is a serious problem in Korea. In particular, while the emission due to construction appears to be very serious among the internal factors, it is necessary to manage PM in consideration of the characteristics of construction sites. Accordingly, in this study, a PM management system suitable for construction sites was developed to reduce civil complaints caused by PM and to minimize damage to field workers and nearby residents by supporting the fine dust management system of the state and local governments. The factors to be considered when measuring PM due to the specificity of construction sites were considered, and the system components were developed based on the considerations. As a result, an IoT based construction site PM monitoring system (CPMS) that integrates each component was established.

• 한국지하수토양환경학회지:지하수토양환경
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• 제25권3호
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• pp.65-73
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• 2020

Exposure assessment methodology for outdoor air inhalation pathways (i.e., inhalation of volatile compounds and fugitive dust in outdoor air) was investigated. Default values of several parameters currently used in Korea (e.g., Q/C; inverse value of concentration per unit flux, and frs; soil fraction in PM10) may not be suitable and lack site-specificity, as they have been adopted from the risk assessment guidance of the United States or the Netherlands. Such limitation can be addressed to a certain degree by incorporating the volatilization factor (VF) and the particulate emission factor (PEF) with Box model. This approach was applied to an exposure assessment of a site contaminated with petroleum hydrocarbons in Korea. The result indicated that the suggested methodology led to more accurate site-specific exposure assessment for outdoor inhalation pathways. Further work to establish methodology to determine site-specific Q/C values in Korea needs to be done to secure the reliability of the exposure assessment for outdoor air inhalation pathways.

• The Plant Pathology Journal
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• 제34권3호
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• pp.191-198
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• 2018

Fast and accurate diagnosis is needed to eradicate and manage economically important and invasive diseases like fire blight. Loop-mediated isothermal amplification (LAMP) is known as the best on-site diagnostic, because it is fast, highly specific to a target, and less sensitive to inhibitors in samples. In this study, LAMP assay that gives more consistent results for on-site diagnosis of fire blight than the previous developed LAMP assays was developed. Primers for new LAMP assay (named as DS-LAMP) were designed from a histidine-tRNA ligase gene (EAMY_RS32025) of E. amylovora CFBP1430 genome. The DS-LAMP amplified DNA (positive detection) only from genomic DNA of E. amylovora strains, not from either E. pyrifoliae (causing black shoot blight) or from Pseudomonas syringae pv. syringae (causing shoot blight on apple trees). The detection limit of DS-LAMP was 10 cells per LAMP reaction, equivalent to $10^4$ cells per ml of the sample extract. DS-LAMP successfully diagnosed the pathogens on four fire-blight infected apple and pear orchards. In addition, it could distinguish black shoot blight from fire blight. The $B{\ddot{u}}hlmann$-LAMP, developed previously for on-site diagnosis of fire blight, did not give consistent results for specificity to E. amylovora and on-site diagnosis; it gave positive reactions to three strains of E. pyrifoliae and two strains of P. syringae pv. syringae. It also, gave positive reactions to some healthy sample extracts. DS-LAMP, developed in this study, would give more accurate on-site diagnosis of fire blight, especially in the Republic of Korea, where fire blight and black shoot blight coexist.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1291-1299
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• 2007

Two ${\beta}$-1,4-glucanases (DI and DIII fractions) were purified to homogeneity from the culture filtrate of a cellulolytic bacteria, Cellulomonas sp. CS 1-1, which was classified as a novel species belonging to Cellulomonas uda based on chemotaxanomic and phylogenetic analyses. The molecular mass was estimated as 50,000 Da and 52,000 Da for DI and DIII, respectively. Moreover, DIII was identified as a glycoprotein with a pI of 3.8, and DI was identified as a non-glycoprotein with a pI of 5.3. When comparing the ratio of the CMC-saccharifying activity and CMC-liquefying activity, DI exhibited a steep slope, characteristic of an endoglucanase, whereas DIII exhibited a low slope, characteristic of an exoglucanase. The substrate specificity of the purified enzymes revealed that DI efficiently hydrolyzed CMC as well as xylan, whereas DIII exhibited a high activity on microcrystalline celluloses, such as Sigmacells. A comparison of the hydrolysis patterns for pNP-glucosides (DP 2-5) using an HPLC analysis demonstrated that the halosidic bond 3 from the nonreducing end was the preferential cleavage site for DI, whereas bond 2, from which the cellobiose unit is split off, was the preferential cleavage site for DIII. The partial N-terminal amino acid sequences for the purified enzymes were $^1Ala-Gly-Ser-Thr-Leu-Gln-Ala-Ala-Ala-Ser-Glu-Ser-Gly-Arg-Tyr^{15}$-for DI and $^1Ala-Asp-Ser-Asp-Phe-Asn-Leu-Tyr-Val-Ala-Glu-Asn-Ala-Met-Lys^{15}$-for DIII. The apparent sequences exhibited high sequence similarities with other bacterial ${\beta}$-1,4-glucanases as well as ${\beta}$-1,4-xylanases.

• 한국의류산업학회지
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• 제14권5호
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• pp.701-712
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• 2012

This study examines the various expressions and immanent value of fashion and beauty style based on Craig Owens's Allegory theory. I analyzed four application elements of Borrow, Site Specificity, Accumulation of Strategy, and Hybridization in? Ladygaga's Music Videos. The results are as follows. 'Borrow' presents a kitsch style and playful Pop-art style, transformation of gender from Mini Mouse body suit, telephone headpiece, and can hair. 'Site specificity' presents the temporarity of fashion material through rebirth, aging, natural extinction from the chain over the black jump suit, crime scene tape, and skull-face makeup with masculine style. 'Accumulation of strategy' presents a futuristic chic fashion style from a layered style, retrospective fashion, repetition and duplication in Music Videos. It shows the physical beauty of an Asian warrior style in Poker Face. 'Hybridization' present Cyborg feminism and 'Will of Power' from iPOD LCD glasses and Pyro-Bra. The Pyro-Bra represents how the female body can be used as a weapon in the outfits of Lady Gaga. In addition, Immanent value is as follows. Textual interaction with high art is accomplished through a combination of contemporary social and cultural significance to understand the cultural code and to extend the value. Combined with high culture, popular music genre is accomplished through musical appreciation by a woman in fashion styling and sensual pleasures of the body as a tool to express a sublime advantage. Fashion style is accomplished by overcoming a self-transcendent body image representation. The way in which mutual coupling dismantling, destruction, and uncertainty is to re-launch the static, with a pluralistic context of Textuality.

• 한국의류산업학회지
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• 제14권4호
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• pp.519-531
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• 2012

This study comprehends the various expressions of Lady Gaga's fashion style based on Craig Owens's Allegory theory. This study analyzed four application elements of Borrow, Site Specificity, Accumulate of Strategy, and Hybridization in addition, it studied all aspects of the aesthetic value of Lady GaGa (an influential popular culture icon). It was classified in the external representation of the fashion style for the aesthetic value. The results are summarized as follows: First, 'Borrow' of the singers of the 80's music and fashion style present from her elders and visual homage to shock artists. It influenced her fans with a difference in viewpoint for a star's fashion that subsequently resulted in a deformation of form, playful kitsch style, and mixed gender. Second, 'Site specificity' presents an extreme make over through an intentional and grotesques fashion style to extend physical territory and defenseless. The results remove stereotypes and reveal deconstructive performances. Third, 'Accumulate of strategy' simultaneously presents voluptuous beauty, futurism, and avant-garde style. This shows the countercultural tendency through the random repetition of fashion images and layerd coordination. Finally, 'Hybridization' presents multiful fashion style through a collaboration with world-famous designers and cosmetic brands. She expressed a diverse and complex fashion style composed of an art form that combines a high-tech cyborg image. The aesthetic values of Lady Gaga' fashion style are 'ambivalence virtuality', 'Transcendental mixed gender', 'plural textuality', and 'unexpected play culture'.