YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Cross-reactivity of Amphetamine Analogues in Various Immunoassays

면역분석법에서 암페타민류의 교차 반응성

  • 박미정 (국립과학수사연구소 법과학부) ;
  • 최화경 (국립과학수사연구소 법과학부) ;
  • 최상길 (국립과학수사연구소 법과학부) ;
  • 손행자 (국립과학수사연구소 법과학부) ;
  • 임미애 (국립과학수사연구소 법과학부) ;
  • 정희선 (국립과학수사연구소 법과학부)
  • Published : 2003.10.01
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Abstract

We evaluated four commercially available methamphetamine immunoassays for their relative cross-reactivities of amphetamine analogues in human urine: Abbott TDx, Vitalab Selectra and on-site test kits (Accusign MET, SD bioline MET). High cross-reactivities were shown at designer's drugs such as methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA) and methylenedioxyethylamphetamine (MDEA) in all of the tested immunoassays. Methoxyphenamine, fenfluramine and phentermine were positive in TDx and Selectra, but were not positive in on-site test kits. Pseudoephedrine, norpseudoephedrine, ephedrine, norephedrine, MDMA, MDA, fenfluramine and phentermine were detected by gas chromatography/mass spectrometry(GC/MS) in false positive urines. Since the overall specificity of any of the devices was not 100％, we found it is important to confirm any positive screening test result, so we developed simultaneous determination of amphetamine analogues in urines. After alkalinization of the urine samples with 6-N NaOH, the analytes were extracted using ethyl acetate, derivatized with pentafluoropropyl anhydride (PFPA) prior at GC/MS analysis.

Keywords

References

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