• Molecules and Cells
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• 제25권2호
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• pp.258-264
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• 2008

Deschampsia antarctica is the only monocot that thrives in the tough conditions of the Antarctic region. It is an invaluable resource for the identification of genes associated with tolerance to various environmental pressures. In order to identify genes that are differentially regulated between greenhouse-grown and Antarctic field-grown plants, we initiated a detailed gene expression analysis. Antarctic plants were collected and greenhouse plants served as controls. Two different cDNA libraries were constructed with these plants. A total of 2,112 cDNA clones was sequenced and grouped into 1,199 unigene clusters consisting of 243 consensus and 956 singleton sequences. Using similarity searches against several public databases, we constructed a functional classification of the ESTs into categories such as genes related to responses to stimuli, as well as photosynthesis and metabolism. Real-time PCR analysis of various stress responsive genes revealed different patterns of regulation in the different environments, suggesting that these genes are involved in responses to specific environmental factors.

• Clinical and Experimental Reproductive Medicine
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• 제44권1호
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• pp.52-55
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• 2017

The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39.8% was detected using the laser. In all, five mature oocytes were injected, resulting in four cases of normal fertilization (80%) on day 1. Further, two high-quality day 3 embryos were transferred, which resulted in a singleton pregnancy. Our study demonstrates that completely immotile spermatozoa are worth cryopreserving for further intracytoplasmic sperm injection, which provides a new insight into male fertility preservation in cases of completely immotile spermatozoa.

• 한국임상수의학회지
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• 제34권4호
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• pp.304-306
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• 2017

A 3-year-old Thoroughbred mare was referred to J&C Equine Hospital with gestation day 30 twin pregnancy. On transrectal ultrasonographic examination, two similar sized (28 mm) embryonic vesicles, unilaterally fixed in the uterine horn, were detected. Transvaginal ultrasound-guided aspiration (TUGA) was performed for reduction of one embryonic vesicle. Yolk sac and allantoic cavity fluids from one embryonic vesicle were aspirated by a needle guided by using transvaginal ultrasound. The mare continued normal singleton pregnancy after twin reduction and delivered a foal successfully. This is the first case described the clinical use of TUGA in Korea. Clinical use of TUGA in twin reduction after embryonic fixation is recommended for equine clinicians.

• Clinical and Experimental Reproductive Medicine
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• 제25권2호
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• pp.233-237
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• 1998

We demonstrated that the normal pregnancy and delivery by round spermatid injection (ROSI) into oocytes was achieved from nonobstructive azoospermia patient. In this case, the normal fertilization rate was 50%. All of the two pronuclear stage embryos cleaved and were transferred to the patient's uterus. A singleton pregnancy was achieved and resulted in the birth of normal female infant. This resuJt show that intracytoplasraic injection of round spermatid seems to be new treatment of nonobstmctive azoospermia male infertility. Further research is needed to evaluate the required culture conditions to induce progression of the round spermatid into a more elongated stage.

• 응용통계연구
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• 제30권4호
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• pp.513-528
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• 2017

두 표본 집단이 동일한 분포를 따르는지 비교하기 위해 분포무관 검정이 많이 사용된다. 하지만 여러 검정법을 체계적으로 비교한 연구가 존재하지 않아서 각 검정법의 특성을 고려하여 연구 상황에 맞는 검정법을 선택하기가 어려웠다. 본 연구에서는 이표본 분포 동일성 검정에 해당하는 여러 분포무관 검정법들을 소개하고 체계적인 모의실험을 통해 그 성능을 비교하고자 한다. 두 표본이 각각 (1) 위치, (2) 척도, (3) 왜도, (4) 첨도, (5) 꼬리가중치가 다른 분포에서 추출된 상황에 대해 실험하였다. 실험 결과를 바탕으로 이표본 분포 동일성 검정법 사용에 대한 실용적인 지침을 제시하려고 한다.

• Parasites, Hosts and Diseases
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• 제57권5호
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• pp.481-487
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• 2019

Mitochondrial DNA sequence variability of Spirometra erinaceieuropaei in GenBank was observed by reinvestigation of mitochondrial cox1 and cytb sequences. The DNA sequences were analyzed in this study, comprising complete DNA sequences of cox1 (n=239) and cytb (n=213) genes. The 10 complete mitochondrial DNA sequences of Spirometra species were compared with those of Korea, China and Japan. The sequences were analyzed for nucleotide composition, conserved sites, variable sites, singleton sites and parsimony-informative sites. Phylogenetic analyses was done using neighbor joining, maximum parsimony, Bayesian inference and maximum-likelihood on cox1 and cytb sequences of Spirometra species. These polymorphic sites identified 148 (cox1) and 83 (cytb) haplotypes within 239 and 213 isolates from 3 Asian countries. Phylogenetic tree topologies were presented high-level confidence values for the 2 major branches of 2 Spirometra species containing S. erinaceieuropaei and S. decipiens, and S. decipiens sub-clades including all sequences registered as S. erinaceieuropaei in cox1 and cytb genes. These results indicated that mitochondrial haplotypes of S. erinaceieuropaei and S. decipiens were found in the 3 Asian countries.

• 대한수학회보
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• 제56권5호
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• pp.1235-1255
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• 2019

In this paper, we introduce SR-additive codes as a generalization of the classes of ${\mathbb{Z}}_{p^r}{\mathbb{Z}}_{p^s}$ and ${\mathbb{Z}}_2{\mathbb{Z}}_2[u]$-additive codes, where S is an R-algebra and an SR-additive code is an R-submodule of $S^{\alpha}{\times}R^{\beta}$. In particular, the definitions of bilinear forms, weight functions and Gray maps on the classes of ${\mathbb{Z}}_{p^r}{\mathbb{Z}}_{p^s}$ and ${\mathbb{Z}}_2{\mathbb{Z}}_2[u]$-additive codes are generalized to SR-additive codes. Also the singleton bound for SR-additive codes and some results on one weight SR-additive codes are given. Among other important results, we obtain the structure of SR-additive cyclic codes. As some results of the theory, the structure of cyclic ${\mathbb{Z}}_2{\mathbb{Z}}_4$, ${\mathbb{Z}}_{p^r}{\mathbb{Z}}_{p^s}$, ${\mathbb{Z}}_2{\mathbb{Z}}_2[u]$, $({\mathbb{Z}}_2)({\mathbb{Z}}_2+u{\mathbb{Z}}_2+u^2{\mathbb{Z}}_2)$, $({\mathbb{Z}}_2+u{\mathbb{Z}}_2)({\mathbb{Z}}_2+u{\mathbb{Z}}_2+u^2{\mathbb{Z}}_2)$, $({\mathbb{Z}}_2)({\mathbb{Z}}_2+u{\mathbb{Z}}_2+v{\mathbb{Z}}_2)$ and $({\mathbb{Z}}_2+u{\mathbb{Z}}_2)({\mathbb{Z}}_2+u{\mathbb{Z}}_2+v{\mathbb{Z}}_2)$-additive codes are presented.

• Clinical and Experimental Reproductive Medicine
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• 제48권2호
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• pp.184-187
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• 2021

Radical trachelectomy is a fertility-preserving alternative to radical hysterectomy in carefully selected young women with early-stage cervical cancer. However, in cases with subsequent severe cervical stenosis, assisted reproductive techniques can be difficult. This is a case report of a 34-year-old patient who underwent robot-assisted radical trachelectomy and cerclage for early-stage (IB2) adenosquamous carcinoma. Three months after surgery, the patient underwent ovarian stimulation using a gonadotropin-releasing hormone antagonist protocol. As it was impossible to perform transcervical embryo transfer due to the almost complete absence of the cervical opening, transmyometrial embryo transfer under ultrasound guidance was performed. This resulted in a successful singleton pregnancy. This is the first case of successful pregnancy conceived by in vitro fertilization with transmyometrial embryo transfer in a patient who had previously undergone robot-assisted radical trachelectomy.

• 말소리와 음성과학
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• 제14권3호
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• pp.67-76
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• 2022

This paper presents two experiments examining different gemination behavior of English affixes. Experiment 1 focused on geminates through affixation with im-, un-, -ness, and -ly. The English group articulated geminates with longer absolute and relative durations than singletons for im-, un-, and -ness, but there was no difference for -ly. This suggests that -ly words are more likely to be perceived as whole words, and that -ly is less decomposable. Furthermore, un- geminates exhibited longer absolute and preceding vowel durations than im- geminates, suggesting that im- is more decomposable than un-. However, the Korean group produced geminates with longer absolute and relative durations than singletons for all im-, un-, -ness, and -ly, and produced comparable absolute durations of im- and un- geminates. Experiment 2 investigated different gemination behaviors of locative and negative im- prefixes. The English group showed durational contrast between geminates and singletons only for negative im-, indicating that locative im- is not easily separated from stem. However, the Korean group produced longer absolute and relative durations for geminates than for singletons for both locative and negative im-. According to the findings of Experiments 1 and 2, affix decomposability is less likely to influence Korean speakers' English affix gemination, and spellings may have a greater influence.

• Journal of Preventive Medicine and Public Health
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• 제56권4호
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• pp.348-356
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• 2023

Objectives: We investigated trends in the incidence rate of macrosomia and its association with parental nationalities using Vital Statistics data in Japan. Methods: We used singleton birth data every 5 years from 1995 to 2020. The incidence rate of macrosomia was calculated according to specific attributes (maternal age, infant's sex, parental nationalities, parity, and household occupation) over time (years). In addition, a log-binomial model was used to investigate the relationship between the incidence of macrosomia and the attributes. This study compared Korea, China, the Philippines, Brazil, and other countries with Japan in terms of parental nationalities. "Other countries" indicates countries except for Japan, Korea, China, the Philippines, and Brazil. Results: The study included 6 180 787 births. The rate of macrosomia in Japan decreased from 1.43% in 1995 to 0.88% in 2020, and the decrease was observed across all parental nationalities. The rates for Japanese parents were the lowest values among parental nationalities during the timespan investigated. Multivariate regression analysis showed that mothers from Korea, China, the Philippines, Brazil, and other countries had a significantly higher risk of macrosomia than those from Japan (risk ratio, 1.91, 2.82, 1.59, 1.74, and 1.64, respectively). Furthermore, fathers from China, the Philippines, Brazil, and other countries had a significantly higher risk of macrosomia than those from Japan (risk ratio, 1.66, 1.38, 1.88, and 3.02, respectively). Conclusions: The rate of macrosomia decreased from 1995 to 2020 in Japan for parents of all nationalities, and the risk of macrosomia incidence was associated with parental nationality.