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Molecular Genetic Classification of Phytophthora Species and P. infestans-specific Marker Selection by RAPD Fingerprinting (Phytophthora species의 분자유전학적 분류 및 RAPD fingerprinting을 이용한 P. infestans-specific 분자마커의 선발)

  • Kim, Kyoung-Su;Shin, Whan-Sung;Kim, Hee-Jong;Woo, Su-Jin;Ham, Young-Il;Shin, Kwan-Yong;Lee, Jeong-Oon;Kim, Byung-Sup;Shim, Jae-Ouk;Lee, Min-Woong;Lee, Youn-Su
    • The Korean Journal of Mycology
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    • v.27 no.6 s.93
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    • pp.394-398
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    • 1999
  • Taxonomic and genetic analysis of Phytophthora species belonging to six different morphological groups (GI, GII, GIII, GIV, GV, GVI) was conducted using RAPD method. Amplified fragments ranged $0.3{\sim}3.2$ kb in their molecular weights. Among total of 145 bands, there were 109 polymorphic bands. Seven isolates of P. infestans showed high similarities of $0.92{\sim}0.99$, and P. infestans isolate 3 from potato showed similarities of $0.93{\sim}0.95$ compared with other P. infestans. Among isolates of P. capsici, similarities of $0.77{\sim}0.86$ were observed and they were grouped in 80% level. P. cinnamomi and P. cryptogea isolates which belonging to group GVI showed very similar RAPD fingerprinting pattern. Primers OPA-04, OPA-17, OPA-18, OPA-19, and OPB-12 showed high level of differences among the tested isolates in major bands and molecular weights. The similarity between the isolates was 0.67. P. megasperma and P. sojae in group GV showed similarity of 0.65. These two isolates showed big differences in single major band in reactions with primers OPA-08, OPA-17, and OPA-19. Phytophthora-specific and P. infestans-specific molecular markers were also selected with one of the random primers tested. In reaction with primer OPA-20, all the genus Phytophthora showed common band at 600 bp, and all the P. infestans isolates showed specific band at 680 bp. These markers can be useful for identification of Phytophthora speices or P. infestans. As a result, P. infestans isolated from tomato and/or potato can easily be differentiated from other Phytophthora species with this primer.

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Tissue Culture Method as a Possible Tool to Study Herbicidal Behaviour and Herbicide Tolerance Screening (조직배양(組織培養) 방법(方法)을 이용(利用)한 제초제(除草劑) 작용성(作用性) 및 제초제(除草劑) 저항성(抵抗性) 검정방법(檢定方法) 연구(硏究))

  • Kim, S.C.;Lee, S.K.;Chung, G.S.
    • Korean Journal of Weed Science
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    • v.6 no.2
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    • pp.174-190
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    • 1986
  • A series of laboratory and greenhouse experiments were conducted to find out the possibility of tissue culture and cell culture methods as a tool to study herbicidal behaviour and herbicide tolerance screening from 1985 to 1986 at the Yeongnam Crop Experiment Station. For dehulled-rice culture, pure agar medium was the most appropriate in rice growth campared to other media used for plant tissue culture method. All the media but the pure agar medium resulted in growth retardance by approximately 50% and this effect was more pronounced to root growth than shoot growth. Herbicidal phytotoxicity was enhanced under light condition for butachlor, 2.4-D, and propanil while this effect was reversed for DPX F-5384 and CGA 142464, respectively. And also, herbicides of butachlor, chlornitrofen, oxadiazon, and BAS-514 resulted in more phytotoxic effect when shoot and root of rice were exposed to herbicide than root exposure only while other used herbicides exhibited no significant difference between two exposure regimes. Similar response was obtained from Echinochloa crusgalli even though the degree of growth retardance was much greater. Particularly, butachlor, 2.4-D, chlornitrofen, oxadiaxon, pyrazolate and BAS-514 totally inhibited chlorophyll biosynthesis even at the single contact of root. Apparent cultivar differences to herbicide were observed at the young seedling culture method and dehulled rice cultivars were more tolerant in DPX F-5384, NC-311, pyrazolate and pyrazoxyfen, respectively. For derant than other types or rice cultivar in butachlor, pretilachlor, perfluidone and oxadiazon while Tongil-type rice cultivars were more tolerant in DPXF-5384, NC-311, Pyrazolate and Pyrazoxyfen, respectively. For dehulled rice culture, on the other hand, Japonica-type rice cultivar was less tolerant to herbicides of butachlor, propanil, chlornitrofen and oxadiazon that was reversed trend to young seedling culture test. Cultivar differences were also exhibited within same cultivar type. In general, relatively higher tolerant cultivars were Milyang 42, Cheongcheongbyeo, Samgangbyeo, Chilseoungbyeo for Tongil-type, Somjinbyeo for Japonica-type and IR50 for Indica-type, respectively. The response of callus growth showed similar to dehulled rice culture method in all herbicides regardless of property variables. However, concentration response was much sensitive in callus response. The concentration ranges of $10^{-9}M-10^(-8)M$ were appropriate to distinguish the difference between herbicides for E. crusgalli callus growth. Among used herbicides, BAS-514 was the most effective to E. crusgalli callus growth. Based on the above results, tissue culture method could be successfully used as a tool for studying herbicidal behaviour and tolerance screening to herbicide.

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Scanning Determination & Observation Features by Sex shown in the Process of Acquiring Visual Information - With the Object of Subway Station Hall Space - (시각정보획득과정에 나타난 주사판정과 성별 주시특성 - 지하철 홀 공간을 대상으로 -)

  • Kim, Jong-Ha;Choi, Gae-Young
    • Korean Institute of Interior Design Journal
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    • v.23 no.6
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    • pp.115-124
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    • 2014
  • This study has carried out scanning tests in order to figure out the features of scanning search by sex of space users, with the result of which the validity of data has been estimated. In this research, the scanning patterns were set up for verifying the typology of scanning paths and then the reason for determining scanning paths and the validity of estimation method were reviewed. Since the observation features depends on sex, the analysis of visual activities for acquiring any information in a space will reveal the intention and purpose of space users. The findings by analyzing the features of scanning pattern by sex which were found at the determination of scanning patterns can be defined as the followings. First, for estimating the process of space-information search, the movement distance at each point of continuative-observation data from the angle of eye-movement has been extracted, on the ground of which the fixation and movement of eye have been defined for the establishment of scanning-cut characteristics. Second, the scanning times were estimated for the extraction of effective observation data that would be used for comparative analysis, which showed that men had more data (3,398.2/64.4%) than women (2,998.2/55.6%). This enables the acknowledgment that the scanning cut of men was relatively less, which indicates that men will acquire more information on space than women in the process of observing any space. Third, men's scanning times (58.0 times/2.02 seconds) were less than those of women (71.9 times/1.39 seconds) while the scanning time of the former was longer than that of the latter, which shows the feature that it takes longer for men than women in scanning while the scanning times of the former is less than those of the latter. Fourth, the observation features can be determined that the combination of this result with the predominance character by sex for a general viewpoint to be employed indicates that while men employ mixed-scanning for observation activities to acquire space-information spending for longer time, women, by concentrated-scanning, focus on a single point for shorter time or stay at one location for a considerably long time for space-information acquirement.

Studies on the Potato Virus X and Potato Leaf Roll Virus for Disease-free Seed Potato Production (무병종서 생산을 위한 감자X바이러스 및 엽권바이러스에 관한 연구)

  • Jhung-Il Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.7 no.1
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    • pp.31-63
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    • 1969
  • A series of experiment was carried out to study on the production of disease-free seed potatoes at the Alpine Experiment Station from 1960 to 1968, which initiated a study of comparison on degeneration of plain warm region and high altitude products and the effect of latent potato virus X (PVX) and potato leaf roll virus(PLRV) on degeneration. Particular observations were made on some aspect of the nature of potato virus disease and its control such as concentrations of PVX, range of host plants, physical properties such as concentrations of PYX, range of host plants, physical properties and carrying effect of insects, by investigating 9 different areas of the main potato producing regions (Kimhae, Taegu, Choongju, Taejoen, Suwon, Kwangju, Chonju, Cheju and Chinju). Highly purified anti-serum was separated and tested for control of the virus disease and also various method of prevention and control of PLRV were observed, using cultivation of sprouted seed tubers, early harvesting method, and systemic chemicals. The results obtained are summarized as follows; 1. Potato yield in the plain region decreased by 32.8~66.3% in the first year cultivation of seed potatoes from colder region, and the rate of virus infection was 92.9 to 95.4%. 2. Plants of three families including, 20 species were susceptible to the PVX, and among the plants Salvia officinalis of a habits only was the carrier while the symptom of Digitalis purpurea of Screphulariaceae was masked. Necrosis and ring spot was occurred in most pJants of the Solanaceae and ring spot symptom also was observed in Nicotiana tabacum L. var. White Burley and in N. glutinosa. 3. The 8$C_2$ strain of virus had the following physical properties; thermal inactivation point, 68-$72^{\circ}C$ : dilution inactivation point, above 1, 000, 000 dilution: ageing in vitro, 240-360 days: and ageing in dry plant tissue, 30 days. 4. Myzus persicae and Oxya spp. did not transmit the 8$C_2$ strain of potato virus. 5. Virus was purified through the ammonium sulphate isolating method, and higher titer value, 1/2048 was obtained through anti-serum test. 6. Inhibition Chenopodiacae on the virus infection of potato was remarkable, and inhibition of local lesion host also was observed. 7, By earlier planting of sprouted seed tubers, growth period could be prolonged by 10 to 12 days. 8. Earlier harvest decreased much the rate of virus infection of seed potatoes. 9. According to the results of aphid control trial using systemic soil insecticides at Kangnung and Taekwanlyung, PSP 204, Disyston and Thimet was effective to aphid control. In particular, control effect of twice treatments of PSP 204 was great. 10. Treatmental effect of those chemicals lasted about 60-70 days. However, single foliar application of emulsified chemicals was not effective to potato virus control. 11. The effect of PSP 204, Disyston, and Thimet on the control of potato leaf roll virus was great, particularly in the case of two treatments of PSP 204, at Kangnung as well as at Taekwanlyung. Higher negative correlationship between the control effect of potato leaf roll virus and potato yield was observed showing the value r=-0.85 at Kangnung, and r=-0.87 at Taekwanlyung. 12. Differences in the control effects among PSP 204, Disyston, and Thimet was not noticed.

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New Variety 'Johyun' of Coix lacryma-jobi var. mayuen Stapf with Early Maturity and Short Plant Height (율무 조숙 단간 다수성 신품종 '조현')

  • Jang, Jung-Hee;Yi, Eun-Seob;Choi, Byeong-Yeol;Kim, In-Jae;Park, Jung-Soo;Kim, Sung-Kee;Kim, Hee-Dong
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.3
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    • pp.122-125
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    • 2005
  • ‘Johyun’ was a new adlay variety which was developed from three way crosses of (Suwon-6xOkayama)x UCN300-25 at the Northern Agricultural Research Station, Gyeonggi Province ARES. This variety had purple sheath and green leaf blade in primitive growth stage and green leaf and culm in heading stage. Colors of pistil and seed coat were milky-white and dark-brown, respectively. Plant height of Johyun was 179 cm and shortened 22 cm less than Yulmoo-1 which was tested by check variety. Johyun shortened 8 days of heading date and 15 days of ripening date compared with Yulmoo-1. Yield of Johyun was 320 kg/10a of hulled grains in three regional yield trials and 18% higher than Yulmoo-1. This variety was adaptable to single cropping in central and northern region of Korea.

Spatio-Temporal Distribution Analysis of One-Person Household - The Case of Busan City - (1인가구의 시공간적 분포 분석 - 부산시를 사례로 -)

  • Yoo, Chang-Ju;Nam, Kwang-Woo
    • Journal of the Korean Association of Geographic Information Studies
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    • v.17 no.2
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    • pp.59-71
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    • 2014
  • At present, Korean one-person households have been continuously increased in spite of the reduction of total population. The increasement of one-person household has become a social and institutional issue. It is necessary to response socially and economically to not only changes of housing demand but also the disadvantaged classes such as the socially weak and single elderly household from the national level. In this respect, this research examined the spatial distribution (such as the increasing area, high-density area, and majority area) of one-person household with census data in the city of Busan. The clusters of one-person households were selected by focusing on the spatial distributions by time series changes of 2000, 2005, and 2010 and considering their housing characteristics. In terms of policy efficiency, the clusters of one-person households to be supported by priority were derived by analyzing the census data from 6066 output areas in the city of Busan. As a result, lots of one-person households of juniors were distributed around the university town, office facility, and station service area. Lots of one-person households at middle-aged class were distributed in Busan's original downtown and mountain-side road. Generalizing these characteristics, cluster analysis was conducted. As a result, one-person household dense area in Busan could be classified into four types. This research should be utilized as a counterplan for increasing the housing demand of one-person household or basic data for supporting small housing supply policies in the future.

Response Analysis of RC Bridge Piers due In Multiple Earthquakes (연속지진하중에 의한 철근콘크리트 교량 교각의 응답해석)

  • Lee Do-Hyung;Jeon Jong-Su;Park Tae-Hyo
    • Journal of the Korea Concrete Institute
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    • v.16 no.3 s.81
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    • pp.357-367
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    • 2004
  • In this paper, the effect of cumulative damage for reinforced concrete bridge piers subjected to both single and multiple earthquakes is investigated. For this purpose, selected are three set of accelerograms one of which represents the real successive input ground motions, recorded at the same station with three months time interval. The analytical predictions indicate that piers are in general subjected to a large number of inelastic cycles and increased ductility demand due to multiple earthquakes, and hence more damage in terms of stiffness degradation is expected to occur. In addition, displacement ductility demand demonstrates that inelastic seismic response of piers can significantly be affected by the applied input ground motion characteristics. Also evaluated is the effect of multiple earthquakes on the response with shear. Comparative studies between the cases with and without shear indicate that stiffness degradation and hence reduction in energy dissipation capacity of piers are pronounced due to the multiple earthquakes combined with shear. It is thus concluded that the effect of multiple earthquakes should be taken into account for the stability assessment of reinforced concrete bridge piers.

Rearing Experiment of Coho Salmon, Oncorhynchus kisutch, in Seawater Cage. I. Comparison of Growth Between Underyearling and Age One (해상 가두리에서의 은연어 양식실험 I. 종묘 나이에 따른 성장비교)

  • Kim Pyong-Kih;Myoung Jung-Goo;Kim Jong-Man;Huh Hyung Tak;Kim Hyung Bae
    • Journal of Aquaculture
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    • v.3 no.2
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    • pp.127-133
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    • 1990
  • Growth Comparisons of two age groups of coho salmon, Oncorhynchus kisutch, underyearling and age one, were made at Chungmu Experimental Fish Culture Station from December 17, 1988 to April 26, 1989. During the first 66 days of experimental period, the fish of underyearling group grew from 167.0 g to 633.1 g in average weight and the feed coefficient (FC) and daily growth rate (DGR) were 1.1 and $2.0\%$, respectively. In the same period, the fish of age one group grew from 396.0 g to 854.6 g in average weight and the FC and DGR were 1.1 and $2.0\%$, respectively. During the second 63 days, the fish of underyearling and age one group grew up to 1171.9 g and 1239.7 g respectively. FCs of these two groups were 1.5 and 2.6, respectively and DGRs were $1.0\%$ and $0.7\%$, respectively. Underyearling fish consumed more feed and performed better FC and DGR than age one fish. FC and DGR sharply decreased as the fish weight increased. Therefore under-yearling fish of this species seems better as seeds for seawater cage culture. For this purpose it is necessary to increase smolting rate in the underyearling group fish within a single season.

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Uncertainty Assessment of Single Event Rainfall-Runoff Model Using Bayesian Model (Bayesian 모형을 이용한 단일사상 강우-유출 모형의 불확실성 분석)

  • Kwon, Hyun-Han;Kim, Jang-Gyeong;Lee, Jong-Seok;Na, Bong-Kil
    • Journal of Korea Water Resources Association
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    • v.45 no.5
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    • pp.505-516
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    • 2012
  • The study applies a hydrologic simulation model, HEC-1 developed by Hydrologic Engineering Center to Daecheong dam watershed for modeling hourly inflows of Daecheong dam. Although the HEC-1 model provides an automatic optimization technique for some of the parameters, the built-in optimization model is not sufficient in estimating reliable parameters. In particular, the optimization model often fails to estimate the parameters when a large number of parameters exist. In this regard, a main objective of this study is to develop Bayesian Markov Chain Monte Carlo simulation based HEC-1 model (BHEC-1). The Clark IUH method for transformation of precipitation excess to runoff and the soil conservation service runoff curve method for abstractions were used in Bayesian Monte Carlo simulation. Simulations of runoff at the Daecheong station in the HEC-1 model under Bayesian optimization scheme allow the posterior probability distributions of the hydrograph thus providing uncertainties in rainfall-runoff process. The proposed model showed a powerful performance in terms of estimating model parameters and deriving full uncertainties so that the model can be applied to various hydrologic problems such as frequency curve derivation, dam risk analysis and climate change study.

Genetic Diversity of Korean Cylindrocarpon destructans Based on Virulence Aassay and RAPD Analysis (병원성 검정 및 RAPD 분석에 의한 국내 인삼뿌리썩음병균(Cylindrocarpon destructans)의 유전적 다양성)

  • Seo, Mun-Won;Kim, Sun-Ick;Song, Jeong-Young;Kim, Hong-Gi
    • The Korean Journal of Mycology
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    • v.39 no.1
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    • pp.16-21
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    • 2011
  • Ginseng root rot caused by Cylindrocarpon destructans is one of the most destructive diseases of ginseng(Panax ginseng). We analyzed the features of the species through pathogenicity test and genetic diversity analysis of C. destructans in Korea, for its application as basic data to attempt for effective control. C. destructans isolated from rotted ginseng roots exhibited a variety of colonial colors on media. It was assumed that there may exist genetic diversity in the population by the diversity of pathogenicity among isolates observed when artificially inoculated into ginseng roots. Pathogenicity tests using ex vivo wound inoculation with agar mixture inoculation on ginseng roots were performed similar results as were observed appear to be useful for rapid pathogen inspection. According to RAPD analysis results, Korean C. destructans isolates formed a single genetic group which can be distinguished readily from closely related other fungi. C. destructans group was divided into two small groups. Therefore, we were able to confirm pathogenicity and genetic difference between the isolates in each of the groups of the pathogen.