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http://dx.doi.org/10.4489/KJM.2011.39.1.016

Genetic Diversity of Korean Cylindrocarpon destructans Based on Virulence Aassay and RAPD Analysis  

Seo, Mun-Won (Department of Applied Biology, Chungnam National University)
Kim, Sun-Ick (Keumsan Ginseng & Medicinal Crop Experiment Station)
Song, Jeong-Young (Department of Applied Biology, Chungnam National University)
Kim, Hong-Gi (Department of Applied Biology, Chungnam National University)
Publication Information
The Korean Journal of Mycology / v.39, no.1, 2011 , pp. 16-21 More about this Journal
Abstract
Ginseng root rot caused by Cylindrocarpon destructans is one of the most destructive diseases of ginseng(Panax ginseng). We analyzed the features of the species through pathogenicity test and genetic diversity analysis of C. destructans in Korea, for its application as basic data to attempt for effective control. C. destructans isolated from rotted ginseng roots exhibited a variety of colonial colors on media. It was assumed that there may exist genetic diversity in the population by the diversity of pathogenicity among isolates observed when artificially inoculated into ginseng roots. Pathogenicity tests using ex vivo wound inoculation with agar mixture inoculation on ginseng roots were performed similar results as were observed appear to be useful for rapid pathogen inspection. According to RAPD analysis results, Korean C. destructans isolates formed a single genetic group which can be distinguished readily from closely related other fungi. C. destructans group was divided into two small groups. Therefore, we were able to confirm pathogenicity and genetic difference between the isolates in each of the groups of the pathogen.
Keywords
Cylindrocarpon destructans; Genetic diversity; Ginseng root rot; Pathogenicity test; RAPD analysis;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Rahman, M. and Punja Z. K. 2005. Factors influencing development of root rot on ginseng caused by Cylindrocarpon destructans. Phytopathology 95:1381-1390.   DOI   ScienceOn
2 Rohlf, F. J. 1992. NTSIS-pc, numerical taxonomy and multivariate analysis system. New York: Exeter Publishing, Ltd.
3 Samuels, G. J. and Brayford, D. 1990. Variation in Nectria radicicola and its anamorph Cylindrocarpon destructans. Mycol. Res. 94:433-442.   DOI
4 Seifert, K. A., McMullen, C. R., Yee, D., Reeleder, R. D. and Dobinson, K. F. 2003. Molecular differentiation and detection of ginseng-adapted isolates of the root rot fungus Cylindrocarpon destructans. Phytopathology 93:1533-1542.   DOI   ScienceOn
5 Zinssmeister, C. L. 1918. Ramularia Root rots of ginseng. Phytopathology 8:557-571.
6 Cho, D. H., Park, K. J., Yu, Y. H., Ohh, S. H. and Lee, H. S. 1995. Root-rot development of 2-year old ginseng (Panax ginseng C. A. Meyer) caused by Cylindrocarpon destructans (Zinssm.) Scholten in the continuous cultivation field. Korea J. Ginseng Sci. 19:175-180.   과학기술학회마을
7 Chung, H. S. and C. H. Kim. 1980. Biological Control of ginseng root rot with soil amendment. Proc. The 2nd International Ginseng Symposium 67-74.
8 Dahm, H., and Strzelczyk, E. 1987. Cellulytic and pectolytic activity of Cylindrocarpon destructans (zinns.) Scholt. isolates pathogenic and non-pathogenic to fir (Abies alba Mill) and pine (Pinus sylvestris L.). J. Phytopathology 118:76-83.   DOI
9 Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13.
10 Evans, G., Cartwright, J. B., and White, N. H. 1967. The roduction of a phytotoxin, Nectrolide, by some root-surface isolates of Cylindrocarpon radicicola, Wr. Plant and Soil 26:235-260.   DOI
11 Matsuo T. and Miyazawa., 1969. Cylindrocarpon panacis sp. nov. causing root rot of ginseng. Ann. Trans. Mycol. Soc. Japan. 11:109.
12 Hamelin, R. C., Berube, P., Gignac, M. and Bourassa, M. 1996. Identification of root rot fungi in nursery seedlings by nested multiplex PCR. Appl. Environ. Microbiol. 62:4026-4031.
13 Hildbrand, A. A. 1935. Root rot of ginseng in Ontario caused by members of the genus Ramularia. Can. J. Res. 12:82-114.   DOI
14 Mantiri, F. R., Samuels, G. J., Rahe, J. E. and Honda, B. M. 2001. Phylogenetic relationships in Neonectria species having Cylindrocarpon anamorphs inferred from mitochondrial ribosomal DNA sequences. Can. J. Bot. 79: 337-340.
15 Punja, Z. K. 1997. Fungal pathogens of American ginseng (Panax quinquefolius) in British Columbia. Can. J. Plant Pathol. 19:301-306.   DOI
16 목성균. 2000. 표준영농교본. 인삼재배, pp. 237, 농촌진흥청.
17 목성균, 박귀희. 1981. 인삼연구보고서(재배분야) pp. 263.
18 오승환. 1981. 인삼의 병해: 환경 및 기주조건과 발병과의 관계. 고려인삼학회지. 5:73-84.   과학기술학회마을
19 유성준, 조진웅, 유승헌, 조재성. 1995. 인삼 근부병균 Cylindrocarpon destructans(Zins.) Scholt. 후막포자 발아에 관하여. 한국식물병리학회지 11:182-183.
20 유연현, 오승환. 1993. 인삼 병 연구의 과거와 현재. 고려인삼학회지 17:61-68.   과학기술학회마을
21 유연현, 오승환. 1996. 인삼병. 최신고려인삼(재배편) pp. 197-244. 한국인삼연초연구원.
22 이민웅. 1974. 인삼근부병에 관한 연구(제 보). 세균류의 분포와 환경조건에 관하여. 한국미생물학회지 12:153-158.
23 전남주. 2008. 국내 인삼뿌리썩음병균(Cylindrocarpon destructans)의 유전적 다양성과 새로운 기주범위 탐색. 충남대학교 대학원 석사학위논문.
24 조재성, 김충수. 1994. 고려인삼의 답전윤환 재배에 관한 기초연구. 한국과학재단연구보고서 1-20.
25 정영륜, 김홍진, 오승환, 박규진. 1984. 인삼근부병 억제토양 및 유발토양의 근권환경 비교. 한국식물보호학회지 23:142-146.   과학기술학회마을
26 정후섭. 1979. 인삼의 병. 한국식물병리학회 창립 15주년 기념 연구 논고 pp. 107-144.
27 정후섭, 이인원. 1978. 인삼연작장해 방지책. 전매용역보고서.
28 한국식물병리학회. 2009. 한국식물병명목록 제5판.
29 홍순근, 오승환, 유연현. 1992. 인삼 토양 병해충 방제 및 농약 개발 연구. 한국인삼연초연구소 인삼연구보고서 pp. 121-160.
30 Chung, H. S. 1972. Ginseng disease in Korea. Korea Pharmacognosy 2:73-79.
31 Booth, C. 1966. The genus Cylindrocarpon. CAB Int. Mycol. Inst. Mycol. Papers 104:1-56.
32 김기황, 김상석, 오승환. 1988. 큰 검정풍뎅이 및 참검정풍뎅이 유충에 의한 인삼의 피해발생조사. 고려인삼학회지 12:47-52.