• Title/Summary/Keyword: Single source

Search Result 2,214, Processing Time 0.032 seconds

Isolation and Characterization of Marine Bacterial Strain SH-1 Producing Agar-Degrading Enzymes (한천 분해효소를 생산하는 해양 미생물 SH-1의 분리 및 특성 분석)

  • Lee, Jae-Hag;Lee, Soon-Youl
    • Microbiology and Biotechnology Letters
    • /
    • v.42 no.4
    • /
    • pp.324-330
    • /
    • 2014
  • A marine bacterial strain producing agar-degrading enzymes was isolated from a mud flat in Jeboo-do (Korea) using a selective artificial sea water (ASW) agar plate containing agar as the sole carbon source. The isolate, designated as SH-1, was gram-negative, aerobic, and motile with single polar flagellum. 16S rRNA gene sequence similarity analysis showed the isolate SH-1 had the highest homology (96.5%) to marine bacterium Neiella marina J221. Cells could grow at $28-37^{\circ}C$ but not at $42^{\circ}C$, and the agarase activity of the cell culture supernatant was higher when grown at $28^{\circ}C$ than when grown at $37^{\circ}C$. Cells could grow when concentrations of 1-5% (w/v) NaCl were added to the growth media with the best growth observed at 3% NaCl, and the agardegrading enzyme activity of the cell culture supernatant was best when grown at 3% NaCl-containing growth media under the conditions we examined. The crude enzyme prepared from 48-h culture broth of strain SH-1 exhibited an optimum pH and temperature for agar-degrading activity at 7.0 and $40^{\circ}C$, respectively. Zymogram analysis of the crude supernatant and cell extract showed that strain SH-1 produced at least 3 agar-degrading enzymes with molecular weights of 15, 35, and 52 KD. Thinlayer chromatography (TLC) analysis also suggested that HS-1 produces ${\beta}$-agarase to degrade agarose to neoagarooligosaccharides.

EM Responses of Buried Conductive Pipes Calculated by 3-D Finite Element Method (3차원 FEM 모델링에 의한 수평 도전성 관로의 전자기 반응 특성)

  • Chung Ho-Joon;Jung Hyun-Key;Park Yeong-Sue;Jo Chul-Hyun
    • Geophysics and Geophysical Exploration
    • /
    • v.3 no.2
    • /
    • pp.48-52
    • /
    • 2000
  • We have calculated and analyzed the electromagnetic responses of buried conductive pipes due to a horizontal magnetic dipole source on the pound using a three-dimensional (3-D) finite element method to provide useful guidelines for designing electromagnetic pipe locator and for field operation of the system. For single buried pipe, the horizontal component and the horizontal difference of the vertical component of magnetic field show peaks above the pipe. When comparing the width of response curves of both cases around the peak, horizontal difference of vertical component of magnetic field shows much narrower peak, 2 times narrower at a half of maximum amplitude, than that of horizontal component of magnetic field. Accordingly, we can pinpoint the horizontal location of pipe on the ground more accurately by measuring the horizontal difference of vertical component of magnetic fold. Moreover, it will have a merit in determining the depth of pipe, because the equation for depth estimation is defined just above the pipe. When there are two buried pipes separated by two meters with each other, the response of horizontal difference of vertical component of magnetic field has two separate peaks each of which is located above the pipe whereas horizontal magnetic field response has only one peak above the pipe just below the transmitter. Thus, when there exist more than a buried pipe, measuring the horizontal difference of vertical magnetic field can effectively detect not only the pipe under transmitter but also adjacent ones. The width of response curves also indicates higher resolving ability of horizontal difference of vertical component of magnetic field.

  • PDF

A Study on the Efficient Algorithm for Converting Range Matching Rules into TCAM Entries in the Packet Filtering System (패킷 필터링 시스템에서 범위 규칙의 효율적 TCAM 엔트리 변환 알고리즘 연구)

  • Kim, Yong-Kwon;Cho, Hyun-Mook;Choe, Jin-Kyu;Lee, Kyou-Ho;Ki, Jang-Geun
    • Journal of IKEEE
    • /
    • v.9 no.1 s.16
    • /
    • pp.19-30
    • /
    • 2005
  • Packet classification is defined as the action to match the packet with a set of predefined rules. One of classification is to use Ternary Content Addressable Memory hardware search engine that has faster than other algorithmic methods. However, TCAM has some limitations. One of them is that TCAM can not perform range matching efficiently. A range has to be expanded into prefixes to fit the boundary. In general, the number of expansion could be up to 2w-2, where w is the width of the field. For example, if two range fields with 16 bits are used, there could be up to $30\;{\times}\;30\;=\;900$ expansions for a single rule. In this paper, we describe the novel algorithm for converting range matching rules into TCAM entry efficiently. The number of maximum entry is 2w-4 when using the algorithm. Furthermore, it has also benefit about the negation range. In the result of experimentation, the new scheme practically reduces 14 percent in case that searched fields are source port and destination port number.

  • PDF

The Effect of Information Conditions on Mental Health among Elderly (노인의 정보기기 접근 수준이 정신건강 영역에 미치는 영향)

  • Lee, Yoon-Jung
    • Journal of Digital Convergence
    • /
    • v.11 no.10
    • /
    • pp.17-29
    • /
    • 2013
  • The major aim of this research is to examine the effect of computer and internet literacy and cellular phone possession on depression and suicidal ideation among elderly. This study used data of 2011 national survey results on the elderly life conditions. To determine the effectiveness of computer and internet literacy and cellular phone possession, a total of 6,774 respondents over 60 years of age was selected. The SPSS package was used to analyze the data. Multiple linear regression and logit analysis was run to verify influence of information conditions(computer and internet literacy and cellular phone possession) on depression and suicidal ideation. The results are as follows. First, the elder who is male, younger, has higher education and economic level and lives with spouce is in good information conditions. On the contrary to this, the elder who is female, older, low level of education and economic, single and lives with grandchildren is in information minority group. They have high level of depression and rate of suicidal ideation. Second, computer and internet literacy and cellular phone possession associate with level of depression significantly. Third, computer and internet literacy do not associate with suicidal ideation significantly. The results of this study provide significant source to plan informatization policy and welfare services for socially isolated older people.

Effects of the Light Source of LEDs on the Physiological and Flowering Response of Endangered Plant Silene capitata Kom. (LED광질에 따른 분홍장구채(Silene capitata Kom.)의 생리 및 개화 반응)

  • Park, Jae Hoon;Lee, Eung Pill;Lee, Soo In;Jang, Rae Ha;An, Kyung Ho;You, Young Han
    • Korean Journal of Environment and Ecology
    • /
    • v.30 no.5
    • /
    • pp.821-828
    • /
    • 2016
  • We examed physiological and flowering response of S. capitata, the endangered plant in Korea, under LED light conditions in plant factory to cultivate artificially for conservation. We cultivated S. capitata and measured its physiological responses and the number of flowers under red, blue, white, red+far-red mixed, red+blue mixed, and red+blue+white mixed light. The results showed that its photosynthetic rate and chlorophyll content were recorded relatively high in red+blue+white and red+blue mixed light respectively. Transpiration rate and stomatal conductance appeared relatively high in the white single light while water use efficiency was no difference. Photochemical efficiency of photochemical photosystem II by minimum and maximum chlorophyll fluorescence was the highest in the red+blue+white mixed light condition than other ones. The number of flowers of S. capitata was at its peak under the red light or red+far-red mixed light. Therefore, we conclude that the most efficient way to grow for flowering of S. capitata is to provide red light or red+far-red mixed light in the plant factory.

Seismic exploration for understanding the subsurface condition of the Ilwall-dong housing construction site in Pohang-city, Kyongbook (경북 포항시 일월동 택지개발지구의 지반상태 파악을 위한 탄성파탐사)

  • Seo, Man Cheol
    • Journal of the Korean Geophysical Society
    • /
    • v.2 no.1
    • /
    • pp.45-56
    • /
    • 1999
  • Seismic refracrion and reflection surveys were conducted along an E-W trending track of 482 m long in Ilwall-dong, Pohang. End-on spread was employed as source-receiver configuration with 2 m for both geophone interval and offset. Seismic data were acquired using 24 channels at every shot fired every 2 m along the track. Refraction data were interpreted using equations for multi-horizontal layers. Reflection data were processed in the sequence of trace edit, gain control, CMP sorting, NMO correction, mute, common offset gathering, and filtering to produce a single fold seismic section. There are two layers in shallow subsurface of the study area. Upper layer has the P-wave velocities ranging from 267 to 566 m/s and is interpreted as a layer of unconsolidated sediments. Lower layer has P-wave velocities of 1096-3108 m/s and is interpreted as weathered rock to hard rock. Most of the lower layer classified as soft rock. Upper layer has lateral variations in both P-wave velocity and thickness. The upper layer in the eastern part of the seismic line is 3-5 m thick and has P-wave velocity of 400 m/s in average. The upper layer in the western part is 8-10 m thick and has P-wave velocity of 340 m/s in average. The eastern part is interpreted as unconsolidated beach sand, while the western part is interpreted as infilled soil to develop a construction site. Three fault systems of high angle are imaged in seismic reflection section. It is interpreted that the area between these fault systems are relatively safe. Large buildings should be located in the safe ground condition of no fault and footings should be designed to be in the basement rock of 3-10 m deep below the surface.

  • PDF

Culture Conditions for Hydrogen Production of Enterobacter cloacae YJ-1 (수소생산을 위한 Enterobacter cloacae YJ-1의 배양조건)

  • Lee Ki-Seok;Kang Chang-Min;Chung Seon-Yong
    • KSBB Journal
    • /
    • v.19 no.6 s.89
    • /
    • pp.446-450
    • /
    • 2004
  • We investigated the effective culture conditions of anaerobic bacteria, Enterobacter cloacae YJ-1 on hydrogen production. It was cultured with 60 mL of working volume at $35^{\circ}C$, 120 rpm for 40 h. With culture time, hydrogen production and cell growth increased, but residual glucose and pH decreased. When the $2\%$ of glucose was used as single carbon source, hydrogen production was 975.1 mL/L. To enhance hydrogen productivity, mixed carbon sources of glucose and sucrose were added. The maximum hydrogen production was earned at the mixing ratio of 25:75, and it was 1319.5 mL/L. When we added 50 mM of phosphate to protect the pH drop in culture broth, hydrogen production increased 1.3 times more than that of initial concentration. The organic nitrogen sources were more effective than inorganic nitrogen for hydrogen production. Among organic nitrogen, yeast extract was the most effective and its hydrogen production was 1691.3 mL/L. Among 9 of mineral sources, Ferric citrate and $NaMoO_4$ were especially effective, and their productions were 1782.3 mL/L and 1784.8 mL/L, respectively.

Design and Performance Evaluation of Small Size Counting and Imaging Gamma Probe System (소형 계수용 및 영상용 감마프로브 시스템의 설계와 성능평가)

  • Yang, Myo-Geun;Kwark, Cheol-Eun;Sim, yong-Geol;Kim, Hee-Joung;Choi, Yong;Chung, Jung-Key;Lee, Myung-Chul;Koh, Chang-Soon
    • Journal of Biomedical Engineering Research
    • /
    • v.18 no.3
    • /
    • pp.291-299
    • /
    • 1997
  • As a microimaging device detecting gamma rays emitted from small lesions or tumors during operation, the intraoperative surgical probe has been proposed and is now under development. We have designed a multipurpose portable gamma prove system and evaluated the performance both for the absolute counting purpose of residual radioactivities and for the localizing capability of gamma events using the NaI(Tl) crystal and two types of photomultiplier tubes(PMTs). Counting efficiencies in the range of routine clinical use of radiation dose were measured using the assembly of single channel PMTs and 0.5 inch thick NaI(Tl) crystal of 1 inch diameter. The positioning of gamma events for imaging purpose requires the multiple channel PMTs with appropriate positioning electronics. We have designed a simple and reliable positioning circuit based on the concept of modified Anger. In preliminary experiments using the multiple channel PMT of 3 inch diameter and the dim lighth source, we were able to trace and localize the correct position with reduced positioning error by the use of two multiplier/divider chipset and simplified peripherals. The energy resolutions for the counting gamma probe measured as full width at half maximum(FWHM) for Cs-137, F-18, Tc-99m were 12%, 13%, and 36%, respectively. The spatial resolution for the imaging gamma probe measured as FWHM for green LED was 2.9 mm. The results indicate that the currently developing probe is very promising and could be very useful for many applications in nuclear medicine. Future studies will include developing collimators, improving interface hardwares, and evaluating the system with clinical data.

  • PDF

The optical CT output signal characteristic according to temperature change (온도변화에 따른 광CT의 출력 특성)

  • Son, Hyun-Mok;Ahn, Mi-Kyoung;Heo, Soon-Young;Jeon, Jea-Il;Park, Won-Zoo;Lee, Kwang-Sik;Kim, Jung-Bae;Kim, Min-Soo
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
    • /
    • 2004.05a
    • /
    • pp.29-33
    • /
    • 2004
  • In this paper, we took the basic experiment in order to explore the characteristics of optical CT(optical current transformer) for measuring high current in a superhigh voltage condition using faraday effect and wrote that. We used the 1,310[nm] Laser Diode for the source of light and PIN-Photodiode for receiver. The transmission line of light was composed of the single-mode fiber of 30[m] which could maintain the state of polarization in the optical fiber. The range of current was from 400[A] to 1300[A]. In addition, the temperature ranged from $20[^{\circ}C}]\;to\;50[^{\circ}C]$. In a same experiment condition, a power magnitude increases in proportion as input current is increasing and temperature become low. The maximum ratio of error in temperature of $50[^{\circ}C]$ appears 0.15[%] and the 0.16[%], 1.24[%] and 0.07[%] is ratio of error in respectively $40[^{\circ}C],\;30[^{\circ}C],\;and\;20[^{\circ}C]$.

  • PDF

Electrical Properties of Al3+ and Y3+ Co-doped SnO2 Transparent Conducting Films (Al3+와 Y3+ 동시치환 SnO2 투명전극 박막의 전기적 특성)

  • Kim, Geun-Woo;Seo, Yong-Jun;Sung, Chang-Hoon;Park, Keun-Young;Cho, Ho-Je;Heo, Si-Nae;Koo, Bon-Heun
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.25 no.10
    • /
    • pp.805-810
    • /
    • 2012
  • Transparent conducting oxides (TCOs) have wide range of application areas in transparent electrode for display devices, Transparent coating for solar energy heat mirrors, and electromagnetic wave shield. $SnO_2$ is intrinsically an n-type semiconductor due to oxygen deficiencies and has a high energy-band gap more than 3.5 eV. It is known as a transparent conducting oxide because of its low resistivity of $10^{-3}{\Omega}{\cdot}cm$ and high transmittance over 90% in visible region. In this study, co-doping effects of Al and Y on the properties of $SnO_2$ were investigated. The addition of Y in $SnO_2$ was tried to create oxygen vacancies that increase the diffusivity of oxygen ions for the densification of $SnO_2$. The addition of Al was expected to increase the electron concentration. Once, we observed solubility limit of $SnO_2$ single-doped with Al and Y. $\{(x/2)Al_2O_3+(x/2)Y_2O_3\}-SnO_2$ was used for the source of Al and Y to prevent the evaporation of $Al_2O_3$ and for the charge compensation. And we observed the valence changes of aluminium oxide because generally reported of valence changes of aluminium oxide in Tin - Aluminium binary system. The electrical properties, solubility limit, densification and microstructure of $SnO_2$ co-doped with Al and Y will be discussed.