• Molecules and Cells
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• v.25 no.2
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• pp.258-264
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• 2008

Deschampsia antarctica is the only monocot that thrives in the tough conditions of the Antarctic region. It is an invaluable resource for the identification of genes associated with tolerance to various environmental pressures. In order to identify genes that are differentially regulated between greenhouse-grown and Antarctic field-grown plants, we initiated a detailed gene expression analysis. Antarctic plants were collected and greenhouse plants served as controls. Two different cDNA libraries were constructed with these plants. A total of 2,112 cDNA clones was sequenced and grouped into 1,199 unigene clusters consisting of 243 consensus and 956 singleton sequences. Using similarity searches against several public databases, we constructed a functional classification of the ESTs into categories such as genes related to responses to stimuli, as well as photosynthesis and metabolism. Real-time PCR analysis of various stress responsive genes revealed different patterns of regulation in the different environments, suggesting that these genes are involved in responses to specific environmental factors.

• Journal of Korea Multimedia Society
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• v.23 no.2
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• pp.127-134
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• 2020

In this paper, we propose a map alignment method for maps generated by point-line monocular SLAM. In the proposed method, the information of feature lines as well as feature points extracted from multiple maps are fused into a single map. To this end, the proposed method first searches for similar areas between maps via Bag-of-Words-based image matching. Thereafter, it calculates the similarity transformation between the maps in the corresponding areas to align the maps. Finally, we merge the overlapped information of multiple maps into a single map by removing duplicate information from similar areas. Experimental results show that maps created by different users are combined into a single map, and the accuracy of the fused map is similar with the one generated by a single user. We expect that the proposed method can be utilized for fast imagery map generation.

• Journal of fish pathology
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• v.22 no.3
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• pp.353-366
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• 2009

We constructed a rock bream Oplegnathus fasciatus leukocyte cDNA library and a total of 795 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 795 EST clones, 491 different ESTs showed significant homology to previously described genes while 304 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 121 different sequences were identified as putative bio-defense genes or genes associated with immune response.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.1
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• pp.115-121
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• 2012

The bumblebee Bombus terrestris is widely used in greenhouses to pollinate crops. Here, we report the molecular cloning and characterization of chymotrypsin inhibitor and chitin-binding protein homologs from B. terrestris. Two cDNAs encoding chymotrypsin inhibitor (Bt-CI) and chitin-binding protein (Bt-CBP) homologs were cloned from B. terrestris. Gene sequence analysis showed that Bt-CI gene consists of three exons encoding 75 amino acids, including a predicted 20-amino acid signal peptide, while Bt-CBP consists of two exons encoding 78 amino acids, including a predicted 26-amino acid signal peptide. The mature Bt-CI and Bt-CBP peptides contain ten and six conserved cysteine residues, respectively. Database searches using the deduced sequences of Bt-CI and Bt-CBP showed similarity to those from B. impatiens (96% peptide sequence identities). Bt-CI and Bt-CBP were expressed in both the venom gland and fat body of B. terrestris worker bees. The recombinant Bt-CI and Bt-CBP peptides were expressed in baculovirus-infected insect cells. Taken together, our findings describe the molecular characterization of Bt-CI and Bt-CBP from B. terrestris.

• Molecules and Cells
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• v.26 no.4
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• pp.387-395
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• 2008

A novel Tc1-like transposable element has been identified as a new DNA transposon in the mud loach, Misgurnus mizolepis. The M. mizolepis Tc1-like transposon (MMTS) is comprised of inverted terminal repeats and a single gene that codes Tc1-like transposase. The deduced amino acid sequence of the transposase-encoding region of MMTS transposon contains motifs including DDE motif, which was previously recognized in other Tc1-like transposons. However, putative MMTS transposase has only 34-37% identity with well-known Tc1, PPTN, and S elements at the amino acid level. In dot-hybridization analysis used to measure the copy numbers of the MMTS transposon in genomes of the mud loach, it was shown that the MMTS transposon is present at about $3.36{\times}10^4$ copies per $2{\times}10^9$ bp, and accounts for approximately 0.027% of the mud loach genome. Here, we also describe novel MMTS-like transposons from the genomes of carp-like fishes, flatfish species, and cichlid fishes, which bear conserved inverted repeats flanking an apparently intact transposase gene. Additionally, BLAST searches and phylogenetic analysis indicated that MMTS-like transposons evolved uniquely in fishes, and comprise a new subfamily of Tc1-like transposons, with only modest similarity to Drosophila melanogaster (foldback element FB4, HB2, HB1), Xenopus laevis, Xenopus tropicalis, and Anopheles gambiae (Frisky).

• Journal of fish pathology
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• v.21 no.2
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• pp.129-137
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• 2008

We constructed a black rockfish (Sebastes schlegeli) leukocyte cDNA library and a total of 386 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 386 EST clones, 199 different ESTs showed significant homology to previously described genes while 97 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 38 different sequences were identified as putative bio-defense genes or genes associated with immune response.

• Journal of KIISE:Databases
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• v.35 no.5
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• pp.379-390
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• 2008

Due to the increase of the amount of image data, the demand for searching similar images is continuously increasing. Therefore, many researches about the content-based image retrieval (CBIR) are conducted to search similar images effectively. In CBIR, it uses image contents such as color, shape, and texture for more effective retrieval. However, when we apply CBIR to satellite images which are complex and pose the difficulty in using color information, we can have trouble to get a good retrieval result. Since it is difficult to use color information of satellite images, we need image segmentation to use shape information by separating the shape of an object in a satellite image. However, because satellite images are complex, image segmentation is hard and poor image segmentation results in poor retrieval results. In this paper, we propose a new approach to search similar images without image segmentation for satellite images. To do a similarity search without image segmentation, we define a similarity of an image by considering SIFT keypoint descriptors which doesn't require image segmentation. Experimental results show that the proposed approach more effectively searches similar satellite images which are complex and pose the difficulty in using color information.

• The Plant Pathology Journal
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• v.22 no.3
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• pp.260-264
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• 2006

Talaromyces flavus mediates the transmission of Apple stem grooving virus (ASGV) to several host plants. The ASGV-F carried by T.flavus was partially purified from the fungus. Based on sequence analysis and homology searches, this is closely related to other ASGV strains isolated from host plants. The partially purified viral coat protein (CP) was separated on a 12% SDS-polyacrylamide gel and analyzed by Western blotting with an ASGV anti-serum. A single band at 28 kDa reacted with the ASGV anti-serum. The deduced amino acid sequence of the ORF-l showed conserved domains, including an NTP-binding helicase motif, GFAGSGKT. The amino acid sequences of the helicase and CP showed strong homology to other ASGV strains (98%). All ASGV isolated from plants and fungi had salt bridges composed of the CP and the GFAGSGKT motif of the helicase, which are commonly conserved in plant viruses. These results suggest that ASGV-F is one of ASGV strains isolated from T.flavus based on sequence similarity as well as the serological analysis of CP.

• Journal of Society of Preventive Korean Medicine
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• v.17 no.3
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• pp.31-46
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• 2013

Objective : To find a collaboration strategy between western medicine and traditional Korean medicine (KM), this study aims to figure out the changes in the perception and attitude of medical doctors toward KM through systematic review. Method : Systematic literature searches were performed on six Korean databases. Studies were categorized according to the respondents and question items and analyzed by the context of questions, similarity of respondents and measurement scale. And we analyzed the changes of response regarding to medical doctors' and medical students' perspective and attitude to KM. Results : Eighteen survey studies including attitude of medical doctors and medical students toward KM were selected, which were conducted from 1993 to 2011. Although the attitude toward KM did not show any positive change, medical doctors have had more interest in acupuncture than herbal medicine and appreciated KM's treatment effect for musculoskeletal disease. In spite of little KM education experience, they had intentions for acupuncture education at least. Many medical doctors have listed the unscientific aspect as a major reason why they cannot trust KM. Medical doctors working for cooperative practice showed more positive attitude than other medical doctors and medical students had more positive attitude in general than medical doctors Conclusion : Though the growth of KM service and cooperative practice since 1990s, medical doctors' attitude toward KM seems to become more negative. To improve their attitude, making scientific evidences for KM is required as well as giving more education and treatment experience.

• Journal of KIISE
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• v.44 no.5
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• pp.553-558
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• 2017

With the rapid growth of Big Data, research on extracting meaningful information is being pursued by both academia and industry. Especially, data characteristics derived from analysis, and researcher intention are key factors for search algorithms to obtain accurate output. Therefore, reflecting both data characteristics and researcher intention properly is the final goal of data analysis research. The data analyzed properly can help users to increase loyalty to the service provided by company, and to utilize information more effectively and efficiently. In this paper, we explore various methods of document-evaluation, so that we can improve the accuracy of searching article one of the most frequently searches used in real life. We also analyze the experiment result, and suggest the proper manners to use various methods.