Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Expressed sequence tags analysis of immune-relevant genes in rock bream Oplegnathus fasciatus peripheral leukocytes stimulated with LPS

  • Lee, Jeong-Ho (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Noh, Jae-Koo (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Hyun-Chul (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Park, Choul-Ji (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Min, Byung-Hwa (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Choi, Sang-Jun (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Myeong, Jeong-In (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Park, Hyung-Jun (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Park, Chan-Il (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
  • Published : 2009.12.01
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Abstract

We constructed a rock bream Oplegnathus fasciatus leukocyte cDNA library and a total of 795 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 795 EST clones, 491 different ESTs showed significant homology to previously described genes while 304 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 121 different sequences were identified as putative bio-defense genes or genes associated with immune response.

Keywords

References

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