Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Expressed Sequence Tags Analysis of Black Rockfish (Sebastes schlegeli) Peripheral Leukocytes Stimulated with Con A/PMA or LPS

  • Baeck, Gun-Wook (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Kim, Ju-Won (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Kim, Ki-Hyuk (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Jun, Kwan-Yong (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • An, Geun-Hee (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Park, Chan-Il (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
  • Published : 20080800
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Abstract

We constructed a black rockfish (Sebastes schlegeli) leukocyte cDNA library and a total of 386 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 386 EST clones, 199 different ESTs showed significant homology to previously described genes while 97 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 38 different sequences were identified as putative bio-defense genes or genes associated with immune response.

Keywords

References

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