• Title/Summary/Keyword: Sigma factor

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Cloning and Nucleotide Sequence Analysis of the rpoH Gene from Methylovorus sp. Strain SS1 DSM11726 (Methylovorus sp. Strain SS1 DSM11726으로부터 rpoH 유전자의 클로닝과 염기서열 분석)

  • Eom, Chi-Yong;Song, Seung-Eun;Park, Mi-Hwa;Kim, Young-Min
    • Microbiology and Biotechnology Letters
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    • v.35 no.3
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    • pp.177-183
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    • 2007
  • Using complementation of RpoH deficient E. coli strain A7448, the rpoH gene encoding heat shock sigma factor 32 (${\sigma}^{32}$) from Methylovorus sp. strain SS1 DSM11726 was cloned and sequenced. Sequence analysis of a stretch of 1,796-bp revealed existence of an open reading frame encoding a polypeptide of 284 amino acid (32,006 dalton). Deduced amino acid sequence of the Methylovorus sp. strain SS1 RpoH showed that 59.6%, 39.1% and 51.4% identities with those of Nitrosomonas europaea (${\beta}$-proteobacteria), Agrobacterium tumefaciens ($\alpha$-proteobacteria) and E. coli (${\gamma}$-proteobacteria). The expression level of the functional ortholog of RpoH of Methylovorus sp. strain SS1 was increased transiently after heat induction, further indicating that it functions as a heat shock sigma factor.

Temperature Dependence of the Electrical Activation Energy and Defect Density in Undoped Amorphous Germanium (a-Ge : H) (비정질 게르마늄(a-Ge : H)의 전기전도 활성화에너지 및 결함밀도의 온도의존성)

  • Jo, U-Seong;Yu, Jong-Hun
    • Korean Journal of Materials Research
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    • v.5 no.6
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    • pp.639-643
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    • 1995
  • The temperature dependence of the dark conductivity was studied on undoped hydrogenated amorphous germanium (a-Ge : H) over the range from 297 to 423 K. The pre-exponential factor $\sigma$$\_$0/ and activation energy E$\_$C/-E$\_$F/ are determined by an Arrhenius plot. The Arrhenius plot of the electrical conductivity shows a kink around the kink temperature and then is composed of two exponential functions. The obtained statistical shift ${\gamma}$$\_$F/ was about 8.65${\times}$10$\^$-3/eV/K and the pre-exponential factor $\sigma$$\_$0/ was about 2$\Omega$$\^$-1/cm$\^$-1/. A temperature dependent defect density is numerically calculated from the conductivity data. A change of the defect density is observed in the factor of about two in the range of the experimental temperature.

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Computer Simulation of Ionic Conductivity Application to Glassy Solid Electrolytes by Monte-Carlo Method (Monte Carlo 방법에 의한 유리 고체전해질의 이온전도도에 관한 전산 모사)

  • 최진삼;서양곤;강은태
    • Journal of the Korean Ceramic Society
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    • v.31 no.3
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    • pp.241-248
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    • 1994
  • The ionic conductivity in glassy systems were calculated as functions of temperature and ion concentration using Monte-Carol method considering interaction between neighbouring ion-site occupancies, {{{{ rho }}'s. Also the vacancy availability factor, V, the effective jump frequency factor, W, and the charge correlation factor, fc, have been investigated. The Arrhenius plot could be obtained from the ln {{{{ sigma }}T vs. 1/T* plots and was in exellent agreement with the experimental observations. The effects of the various types of potential well on the ionic conductivity have been considered. The activation energy Eg for ion motion in the glass was 1.3│ε│from the ln {{{{ sigma }}T vs. 1/T* plots.

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Method determining level of Noise Factor of Taguchi Method under various probability distribution (다양한 확률분포 하에서 다구찌 기법의 잡음인자 수준 정하는 기법)

  • Ree, Sang-Bok
    • Journal of Korean Society for Quality Management
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    • v.37 no.4
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    • pp.10-15
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    • 2009
  • In this paper, we propose new methods which is to determine level of noise factor. Even Taguchi give level of noise factor which is best(or maximum) and worst(or minimum) condition, we give level of noise factor which is representative value by observing noise factor frequency. Sometimes level of noise factor is given one, two and three. We know this method is more fit in real fields.

Study on The System Suitability Test for Alcohols Separation by GCOTC (GCOTC에 의한 알코올류 분리를 위한 시스템 적합성에 관한 연구)

  • Oh, Doe Seok;Kim, Sung Hwa;Lee, Seul;Choi, Jae Gu
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.27 no.2
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    • pp.123-129
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    • 2017
  • Objectives: The purpose of this study is to enhance the application of analytical method of polar solvents(alcohols) by GCOTC (gas chromatography open tubular column) through the system suitability test(SST) to estimate the whole chromatographic system performance(integral part). Methods: To perform the SST, carried out repeatability(n=6) as analytical method of polar solvents by GCOTC, got the retention time($t_R$), standard deviation(${\sigma}_{n-1}$) of $t_R$, baseline width($w_b=4{\sigma}_{n-1}$) and calculated dead time($t_m$) by $v_m=d^2{\pi}L(f/4)$ and $v_m=t_m$ x flow rate. Results: In this experiment, obtained the basic data, there were $t_m=2$ min, methanol($t_R=3.569$, ${\sigma}_{n-1}=0.01$, $w_b=0.04$), ethanol ($t_R=3.892$, ${\sigma}_{n-1}=0.004$, $w_b=0.016$), isopropanol($t_R=4.209$, ${\sigma}_{n-1}=0.004$, $w_b=0.016$). By using these data, calculated the corrected retention time($t_R{^{\prime}}$), capacity factor(k), separation factor(${\alpha}$), number of theoretical plate(n) and resolution($R_s$) for SST and got the good results. Conclusions: Through the SST, could reconfirm the whole chromatographic performance system(integral part) for analytical method of polar solvents by GCOTC. Therefore, this analytical method expect to be widely applied at the related areas.

Sigma Delta Decimation Filter Design for High Resolution Audio Based on Low Power Techniques (저전력 기법을 사용한 고해상도 오디오용 Sigma Delta Decimation Filter 설계)

  • Au, Huynh Hai;Kim, SoYoung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.49 no.11
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    • pp.141-148
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    • 2012
  • A design of a 32-bit fourth-stage decimation filter decimation filter used in sigma-delta analog-to-digital (A/D) converter is proposed in this work. A four-stage decimation filter with down-sampling factor of 512 and 32-bit output is developed. A multi-stage cascaded integrator-comb (CIC) filter, which reduces the sampling rate by 64, is used in the first stage. Three half-band FIR filters are used after the CIC filter, each of which reduces the sampling rate by two. The pipeline structure is applied in the CIC filter to reduce the power consumption of the CIC. The Canonic Signed Digit (CSD) arithmetic is used to optimize the multiplier structure of the FIR filter. This filter is implemented based on a semi-custom design flow and a 130nm CMOS standard cell library. This decimation filter operates at 98.304 MHz and provides 32-bit output data at an audio frequency of 192 kHz with power consumption of $697{\mu}W$. In comparison to the previous work, this design shows a higher performance in resolution, operation frequency and decimation factor with lower power consumption and small logic utilization.

Improvement of Synthesis Construction Plan through 6sigma (6시그마를 통한 종합시공계획서 작성 프로세스 개선)

  • Baek, Ho-Jin;Yoo, Chang-Kyu;Lee, Yoon-Sun;Kim, Jae-Jun
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2007.11a
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    • pp.613-617
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    • 2007
  • To achieve project successfully in domestic construction site, pre-management of risk is important factor. Synthesis Construction Document made early construction is representative as means of pre-management of risk. But, Synthesis Construction Document is not working properly for pre-management of risk because the level is low as Synthesis Construction Document is made formally and the time is too late to pre manage the risk for various factor. A epoch-making method that differ with existing need to solve this deep-rooted problem of domestic construction site. So, this study is analyzing to achieve subject to heighten level Synthesis Construction Document and shorten creation period with "S"construction company(introducing 6sigma by tool of management innovation 6 sigma)'s data and offering an opportunity that heightens effectiveness and goes forward and aims possibility about construction site suitability of 6sigma by confirming Pilot Test.

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Unscented Kalman Filter with Multiple Sigma Points for Robust System Identification of Sudden Structural Damage (다중 분산점 칼만필터를 이용한 급격한 구조손상 탐지 기법 개발)

  • Se-Hyeok Lee;Sang-ri Yi;Jin Ho Lee
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.36 no.4
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    • pp.233-242
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    • 2023
  • The unscented Kalman filter (UKF), which is widely used to estimate the states of nonlinear dynamic systems, can be improved to realize robust system identification by using multiple sigma-point sets. When using Kalman filter methods for system identification, artificial noises must be appropriately selected to achieve optimal estimation performance. Additionally, an appropriate scaling factor for the sigma-points must be selected to capture the nonlinearity of the state-space model. This study entailed the use of Bouc-Wen hysteresis model to examine the nonlinear behavior of a single-degree-of-freedom oscillator. On the basis of the effects of the selected artificial noises and scaling factor, a new UKF method using multiple sigma-point sets was devised for improved robustness of the estimation over various signal-to-noise-ratio values. The results demonstrate that the proposed method can accurately track nonlinear system states even when the measurement noise levels are high, while being robust to the selection of artificial noise levels.

On Reliability and Comparison of $J_{Rice}$-Resistance considering Optimal Strength Ratio and $J_{\delta}$-Resistance Curves converted from CTOD using Appropriate Strength chosen according to Strain Hardening Level (강도비를 적용한 Rice-저항곡선과 변형경화를 고려한 $J_{\delta}$-저항곡선과의 비교)

  • 장석기
    • Journal of Advanced Marine Engineering and Technology
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    • v.26 no.1
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    • pp.59-67
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    • 2002
  • The comparison of $J_{Rice}$-resistance considering a few strength ratio in Rice J-integral formula and $J_{\delta}$-resistance curves converted from experimental CTOD using appropriate strength chosen according to strain hardening level, n=10.6 (A533B steel) and n=8.1 (BS4360 steel) is carried out. The optimal dimensionless strength ratio like the factor of revision, (see full text)reflecting strain hardening level in Rice\`s experimental formula is found out and the reliability of appropriate reference strength chosen according to strain hardening level in different materials is investigated through doing that CTOD is transformed from $J_{\delta}$-integral using relationship between J-integral and CTOD. The results are as follows; 1) The optimal factor of revision is when m equals to 3 in (see full text) for Rice's and the above optimal factor of revision multiplies by coefficient, η in Rice's experimental formula instead of n=2, 2) and the pertinent reference strength for high strain hardening material like BS4360 steel is ultimate strength, $\sigma_{u}$ and for material like A533B steel is ultimate-flow strength, $\sigma_{u-f}$. The incompatible of the behavior of both experimental J-resistance curves using Rice's formula and CTOD-resistance curves for A533B and BS4360 steel by Gordon, et al., could be corrected using the optimal factor of revision in Rice\`s and the pertinent reference strength in J=$m_{j}$${\times}$$\sigma_{i}$${\times}$CTOD.

Semi-Empirical MO Calculations on ${\pi}$-Nonbonded and ${\sigma}$-Conjugative Interactions (반경험적 분자궤도함수 계산법에 의한 ${\pi}$-비결합 및 ${\sigma}$-컨쥬게이션 상호작용에 관한 연구)

  • Ikchoon Lee;Young Gu Cheun;Kiyull Yang;Wang Ki Kim
    • Journal of the Korean Chemical Society
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    • v.26 no.4
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    • pp.195-204
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    • 1982
  • Semi-empirical MO calculations, EHT, CNDO/2, MINDO/3, and MNDO met hods, were performed on various geometries of n-butane, n-alkyl radical and tetramethylene diracal (triplet) in order to compare eigenvalue and eigenvector properties with those obtained by STO-3G method. All methods predicted the same relative order of stabilities of various geometries for n-butane; geometrical preferences were found to be dominated by one-electron factor, ${\pi}$-orbital energy changes being more impotant in the semi-empirical methods. The hyperconjugative energy changes accompanying structural changes from $(n-{\sigma}{\ast})_{trans}$ to (n-{\sigma}{\ast})cis were underestimated in the EHT, CNDO/2 and MINDO/3, whereas those were overestimated in the MNDO. The net destabilizing effect of $(n-{\sigma}{\ast})_{trans}$ structure was mainly due to the large internuclear energy involved in the structure. Through-space interaction between $n_1$ and $n_2$ orbitals of diradical caused energy gap narrowing of ${\Delta}E_{sp}$ and ${\Delta}{\varepsilon}={\varepsilon}_0$-${\varepsilon}_{av}$; through-space interaction had opposing effect to that of through-bond interaction. Due to the less severe neglect of differential overlaps in the MNDO, this energy gap narrowing effect appeared amplified in the MNDO. In general orbital properties were found to be reproduced satisfactorily, but eigenvalue properties were not, in all the semi-empirical methods especially when ${\sigma}-{\sigma}{\ast}$ and n-$n-{\sigma}{\ast}$interactions were involved.

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