• Title/Summary/Keyword: Shock Stability

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Supersonic flow bifurcation in twin intake models

  • Kuzmin, Alexander;Babarykin, Konstantin
    • Advances in aircraft and spacecraft science
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    • v.5 no.4
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    • pp.445-458
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    • 2018
  • Turbulent airflow in channels of rectangular cross section with symmetric centerbodies is studied numerically. Shock wave configurations formed in the channel and in front of the entrance are examined. Solutions of the unsteady Reynolds-averaged Navier-Stokes equations are obtained with finite-volume solvers of second-order accuracy. The solutions demonstrate an expulsion/swallowing of the shocks with variations of the free-stream Mach number or angle of attack. Effects of the centerbody length and thickness on the shock wave stability and flow bifurcation are examined. Bands of the Mach number and angle of attack, in which there exist non-unique flow fields, are identified.

Prediction of Dynamic Stability Derivatives Using Unsteady Euler Equations (비정상 Euler 방정식을 이용한 동안정 미계수 예측)

  • Park Soo Hyung;Kim Yoonsik;Kwon Jang Hyuk
    • 한국전산유체공학회:학술대회논문집
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    • 2001.05a
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    • pp.78-84
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    • 2001
  • A dual-time stepping algorithm combined with a parallelized multigrid DADI method is presented to predict the dynamic damping coefficients. The Basic Finner model is chosen to validate the prediction capability of the present unsteady Euler method. The linearity of the pitch- and roll-damping coefficients is shown in the low angular rates and the interesting large drop and stiff increment in transonic region for roll-damping coefficients are explained in detail. Through the analysis for the pressure distributions at Mach number 1.0 to 1.2, the sudden drop results from the normal shock and the stiff increment of roll-damping reflects the transition of the normal shock to the oblique shock. The results also show that the Euler equations can give the damping coefficients with a comparable accuracy.

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Cold Shock Response and Low Temperature Stable Transcript of DEAD-box RNA Helicase in Bacillus subtilis (DEAD-box RNA Helicase 유전자가 결핍된 Bacillus subtilis의 저온 충격 반응성과 저온 안정성 전사물)

  • Oh, Eun-Ha;Lee, Sang-Soo
    • Korean Journal of Microbiology
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    • v.47 no.4
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    • pp.289-294
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    • 2011
  • We investigated the cold shock sensitivity of DEAD-box RNA helicase gene deleted strains of in Bacillus subtilis CU1065. To understand cold shock effects, cells were cultivated at $37^{\circ}C$ to log phase ($O.D_{600}$=0.5-0.6) and then temperature was shifted to $15^{\circ}C$. Cold shock slow down the growth rate of wild type and deleted strains of DEAD-box RNA helicase gene (ydbR, yfmL, yqfR, deaD). The growth rate of ydbR deleted strain is 5 times severely reduced compared to that of wild type strain (CU1065). But the growth rate of other three (yfmL, yqfR, deaD) deleted strains is nearly equal to the growth rate of wild type. Compared to $37^{\circ}C$, the amount of ydbR and yqfR mRNA transcripts are increased at the growth temperature of $15^{\circ}C$. On the other hands the mRNA transcripts of yfmL and deaD are not changed at both conditions of $37^{\circ}C$ and $15^{\circ}C$. Upon cold shock treatment ydbR mRNA transcript is clearly increased. After treatment of rifampicin (bacteria transcription inhibitor) the amount of ydbR mRNA was measured. Temperature shift from $37^{\circ}C$ to $15^{\circ}C$ and rifampicin treatment showed slowly decay of ydbR mRNA. But at $37^{\circ}C$ and rifampicin treatment ydbR mRNA is rapidly reduced. These results showed that cold shock induction of ydbR mRNA resulted from the stability of ydbR mRNA and not from the transcription induction of ydbR. In relation to these results, we found the cold box element of csp (cold shock protein gene) in 5' untranslated region of ydbR gene. Cold shock induction of ydbR is caused by the stability of ydbR mRNA like the stability of csp mRNA.

Spacing Method Suitable for Flexible Cholesteric LCDs

  • Yoon, Hyun-Sub;Min, Kwan-Sik;Kwon, Soon-Bum
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07a
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    • pp.484-487
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    • 2005
  • In order to find out LC cell spacing method suitable for flexible cholesteric LCDs with high mechanical stability, we carried out bending and mechanical shock tests by using various spacing techniques: bead spacer, photo spacer, polymer wall with bead spacer and polymer wall with photo spacer. As a result, it was found that the spacing method using polymer wall incorporated with photo spacer is the best for flexible cholesteric LCDs in terms of mechanical stability. The investigation is discussed in detail.

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Development of Encapsulated Media for Ammonia Removal (암모니아 가스 제거를 위한 포괄고정화 담체 개발)

  • Jeong, Mi-young;Namgung, Hyeong Kyu;Song, Ji Hyeon;Hwang, Sun-Jin
    • Journal of Korean Society on Water Environment
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    • v.25 no.2
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    • pp.306-310
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    • 2009
  • Packed-bed reactor for removing ammonia was tested at different loading rates. Nitrifiers for ammonia removing was encapsulated in gel media which consisted of polyethlene glycol, alginate and activated carbon. The removal efficiency was nearly 100% when ammonia loading was $12g/m^3/hr$, and the maximum elimination capacity (EC) achieved on this study was $18g/m^3/hr$. The initial microbial portion of nitrifiers in the media was about 82% and it was increased to more than 90% at the end of the operation. Short-term shock loading test was carried out to survey the stability of the media. The inlet loading rates were varied from 2 to $20g/m^3/hr$. The packed-bed reactor overcame the shock loading i.e. removal efficiency recovered rapidly from about 80% to almost 100% within 6 hrs. The results of Live/Dead cell test showed that nitrifiers maintained there activity in the encapsulated media during the test and also against ammonia shock load.

Thermal Durability of Al2TiO5-Mullite Composites and Its Correlation with Microstructure

  • Kim, Hyung-Chul;Lee, Dong-Jin;Kweon, Oh-Seong;Kim, Ik-Jin
    • Journal of the Korean Ceramic Society
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    • v.42 no.8 s.279
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    • pp.532-536
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    • 2005
  • Thermal shock resistance of structural ceramics is a property that is difficult to quantity, and as such is usually expressed in terms of a number of empirical resistance parameters. These are dependant on the conditions imposed, but one method that can be used is the examination of density, Young's modulus and thermal expansion retention after quenching. For high temperature applications, long-annealing thermal durability, cycle thermal stability and residual mechanical properties are very important if these materials are to be used between $1000^{\circ}C$ and $1300^{\circ}C$. In this study, an excellent thermal shock-resistant material based on $Al_2TiO_5-mullite$ composites of various compositions was fabricated by sintering reaction from the individual oxides and adjusting the composition of $Al_2O_3TiO_2/SiO_2$ ratios. The characterization of the damage induced by thermal shock was done by measuring the evolution of the Young's modulus using ultrasonic analysis, density and thermal expansion coefficients.

Expression Profile of Heat Shock Protein Gene Transcripts (HSP70 and HSP90) in the Nerve Ganglia of Pacific abalone, Haliotis discus hannai Exposed to Thermal Stress

  • Sukhan, Zahid Parvez;Kho, Kang Hee
    • Journal of Marine Life Science
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    • v.5 no.2
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    • pp.92-98
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    • 2020
  • Heat shock proteins (HSPs) are highly conserved cellular proteins that contribute to adaptive responses of organisms to a variety of stressors. In response to stressors, cellular levels of HSPs are increased and play critical roles in protein stability, folding and molecular trafficking. The mRNA expression pattern of two well-known heat shock protein transcripts, HSP70 and HSP90 were studied in two tissues of nerve ganglia, cerebral ganglion and pleuropedal ganglion of Pacific abalone (Haliotis discus hannai). It was observed that both HSP70 and HSP90 transcripts were upregulated under heat stress in both ganglion tissues. Expression level of HSP70 was found higher than HSP90 in both ganglia whereas cerebral ganglion showed higher expression than pleuropedal ganglion. The HSP70 and HSP90 showed higher expression at Day-1 after exposed to heat stress, later decreased at Day-3 and Day-7 onwards. The present result suggested that HSP70 and HSP90 synthesize in nerve ganglion tissues and may provide efficient protection from stress.

Purification and Structural Characterization of Cold Shock Protein from Listeria monocytogenes

  • Lee, Ju-Ho;Jeong, Ki-Woong;Kim, Yang-Mee
    • Bulletin of the Korean Chemical Society
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    • v.33 no.8
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    • pp.2508-2512
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    • 2012
  • Cold shock proteins (CSPs) are a family of proteins induced at low temperatures. CSPs bind to single-stranded nucleic acids through the ribonucleoprotein 1 and 2 (RNP 1 and 2) binding motifs. CSPs play an essential role in cold adaptation by regulating transcription and translation via molecular chaperones. The solution nuclear magnetic resonance (NMR) or X-ray crystal structures of several CSPs from various microorganisms have been determined, but structural characteristics of psychrophilic CSPs have not been studied. Therefore, we optimized the purification process to obtain highly pure Lm-Csp and determined the three-dimensional structure model of Lm-Csp by comparative homology modeling using MODELLER on the basis of the solution NMR structure of Bs-CspB. Lm-Csp consists of a ${\beta}$-barrel structure, which includes antiparallel ${\beta}$ strands (G4-N10, F15-I18, V26-H29, A46-D50, and P58-Q64). The template protein, Bs-CspB, shares a similar ${\beta}$ sheet structure and an identical chain fold to Lm-Csp. However, the sheets in Lm-Csp were much shorter than those of Bs-CspB. The Lm-Csp side chains, E2 and R20 form a salt bridge, thus, stabilizing the Lm-Csp structure. To evaluate the contribution of this ionic interaction as well as that of the hydrophobic patch on protein stability, we investigated the secondary structures of wild type and mutant protein (W8, F15, and R20) of Lm-Csp using circular dichroism (CD) spectroscopy. The results showed that solvent-exposed aromatic side chains as well as residues participating in ionic interactions are very important for structural stability. Further studies on the three-dimensional structure and dynamics of Lm-Csp using NMR spectroscopy are required.

Effects of Porosity on Durability in a Porous Nozzle for Continuous Casting (연속주조용 Porous Nozzle의 기공율이 내구성에 미치는 영향)

  • Yoon, Sanghyeon;Cho, Mun-Kyu;Jeong, Doo Hoa;Lee, Heesoo
    • Korean Journal of Metals and Materials
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    • v.48 no.7
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    • pp.625-629
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    • 2010
  • This study investigates the effects of porosity on the thermal stability and the thermal shock resistance of a porous nozzle used for blowing an inert gas. The samples of $Al_2O_3-SiO_2-ZrO_2$ system, which had the apparent porosity of 16~30% and bulk density of $2.6{\sim}3.2g/cm^3$, were prepared by adding different graphite contents (5, 10, 20 wt%) as a pore-forming agent. The thermal shock test was conducted at ${\Delta}T=500$, 1000, and $1400^{\circ}C$ also and the thermal stability was also carried out at 1550, 1600, and $1650^{\circ}C$ for 5 hrs. The specimen contained 10 wt% graphite had uniform pore size distribution, whereas the specimen with 20 wt% graphite showed non-uniform pore size distribution. As a result of thermal shock test, the specimen containing 10 wt% graphite appears to have higher mechanical strength than the other specimens (5, 20 wt% graphite). Both the 5 wt% and 20 wt% graphite specimens developed a non-uniform pore size distribution and cracks that were generated by intensive thermal stress.