Journal of Marine Life Science (한국해양생명과학회지)

The Korean Society of Marine Life Science (한국해양생명과학회)
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Expression Profile of Heat Shock Protein Gene Transcripts (HSP70 and HSP90) in the Nerve Ganglia of Pacific abalone, Haliotis discus hannai Exposed to Thermal Stress

  • Sukhan, Zahid Parvez (Department of Fisheries Sciences, Faculty of Aquatic Biology, Chonnam National University) ;
  • Kho, Kang Hee (Department of Fisheries Sciences, Faculty of Aquatic Biology, Chonnam National University)
  • Received : 2020.11.24
  • Accepted : 2020.12.14
  • Published : 2020.12.16
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Abstract

Heat shock proteins (HSPs) are highly conserved cellular proteins that contribute to adaptive responses of organisms to a variety of stressors. In response to stressors, cellular levels of HSPs are increased and play critical roles in protein stability, folding and molecular trafficking. The mRNA expression pattern of two well-known heat shock protein transcripts, HSP70 and HSP90 were studied in two tissues of nerve ganglia, cerebral ganglion and pleuropedal ganglion of Pacific abalone (Haliotis discus hannai). It was observed that both HSP70 and HSP90 transcripts were upregulated under heat stress in both ganglion tissues. Expression level of HSP70 was found higher than HSP90 in both ganglia whereas cerebral ganglion showed higher expression than pleuropedal ganglion. The HSP70 and HSP90 showed higher expression at Day-1 after exposed to heat stress, later decreased at Day-3 and Day-7 onwards. The present result suggested that HSP70 and HSP90 synthesize in nerve ganglion tissues and may provide efficient protection from stress.

Keywords

Acknowledgement

This research was a component of a project titled 'Development of technology for abalone aquaculture using sperm cryopreservation (Grant No. 2018-2129)' funded by the Ministry of Oceans and Fisheries, Republic of Korea.

References

  1. Batulan Z, Taylor DM, Aarons RJ, Minotti S, Doroudchi MM, Nalbantoglu J, Durham HD. 2006. Induction of multiple heat shock proteins and neuroprotection in a primary culture model of familial amyotrophic lateral sclerosis. Neurobiol Dis 24: 213-225. https://doi.org/10.1016/j.nbd.2006.06.017
  2. Bittner GD. 1988. Long term survival of severed distal axonal stumps in vertebrates and invertebrates. Am Zool 28: 1165-1179. https://doi.org/10.1093/icb/28.4.1165
  3. Bittner GD. 1991. Long-term survival of anucleate axons and its implications for nerve regeneration. Trends Neurosci 14: 188-193. https://doi.org/10.1016/0166-2236(91)90104-3
  4. Bohush A, Bieganowski P, Filipek A. 2019. HSP90 and Its Co-Chaperones in Neurodegenerative Diseases. Int J Mol Sci 20: 4976. https://doi.org/10.3390/ijms20204976
  5. Breitburg D, Levin LA, Oschlies A, Gregoire M, Chavez FP, Conley DJ, Jacinto GS. 2018. Declining oxygen in the global ocean and coastal waters. Science 359: 6371.
  6. Cheng P, Liu X, Guofan Zhang G, Jianguo He J. 2007. Cloning and expression analysis of a HSP70 gene from Pacific abalone (Haliotis discus hannai). Fish Shellfish Immu 22: 77-87. https://doi.org/10.1016/j.fsi.2006.03.014
  7. Choi KS, Kim BH, Lim HK. 2015. Current status of abalone aquaculture in Korea. In: World Aquaculture 2015 - Meeting Abstract. [online] https://www.was.org/meetings/ShowAbstract.aspx?Id=35639
  8. Craig EA, Weissman JS, Horwich AL. 1994. Heat shock proteins and molecular chaperones: mediators of protein conformation and turnover in the cell. Cell 78: 365-372. https://doi.org/10.1016/0092-8674(94)90416-2
  9. Ding J, Li L, Wu F, Zhang G. 2016. Effect of chronic temperature exposure on the immunity of abalone, Haliotis discus hannai. Aquac Res 47: 2861-2873. https://doi.org/10.1111/are.12736
  10. Foster NL, Lukowiak K, Henry TB. 2015. Time-related expression profiles for heat shock protein gene transcripts (HSP40, HSP70) in the central nervous system of Lymnaea stagnalis exposed to thermal stress. Commun Integr Biol 8: e1040954. https://doi.org/10.1080/19420889.2015.1040954
  11. Gordon H, Cook P. 2004. World abalone fisheries and aquaculture update: supply and market dynamics. J Shellfish Res 23: 935-939.
  12. Hara M, Sekino M. 2005. Genetic difference between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus populations: microsatellite-based population analysis in Japanese abalone. Fish Sci 71: 754-766. https://doi.org/10.1111/j.1444-2906.2005.01025.x
  13. Jolly C, Morimoto RI. 2000. Role of the heat shock response and molecular chaperones in oncogenesis and cell death. J Natl Cancer Inst 92: 1564-1572. https://doi.org/10.1093/jnci/92.19.1564
  14. Jolly ER, Liang S, Yu X, Wood D. 2018. HSP70, HSP90A, and HSP90B are differentially regulated in response to thermal, osmotic and hypoxic stressors. Ann Exp Mol Biol 1: 000101.
  15. Kang HY, Lee YJ, Song WY, Kim TI, Lee WC, Kim TY, Kang CK. 2019. Physiological responses of the abalone Haliotis discus hannai to daily and seasonal temperature variations. Sci Rep 9: 1-13.
  16. Keppler BR, Grady AT, Jarstfer MB. 2006. The biochemical role of the heat shock protein 90 chaperone complex in establishing human telomerase activity. J Biol Chem 281: 19840-19848. https://doi.org/10.1074/jbc.M511067200
  17. Liang S, Yu X, Wood D, Jolly ER. 2013. Hsp70 and HSP90 are differentially expressed in crayfish muscle and neurons after heat stress. Res Rep Bio 4: 41-51
  18. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25: 402-408. https://doi.org/10.1006/meth.2001.1262
  19. Lv DH. 1978. A study on the Haliotidae from the coast of China. Studia Marina Sinic 14: 89-100.
  20. Magrane J, Smith RC, Walsh K, Querfurth HW. 2004. Heat shock protein 70 participates in the neuroprotective response to intracellularly expressed beta-amyloid in neurons. J Neurosci 24: 1700-1706. https://doi.org/10.1523/JNEUROSCI.4330-03.2004
  21. Manzerra P, Brown IR. 1992. Expression of heat shock genes (HSP70) in the rabbit spinal cord: localization of constitutive and hyperthermia-inducible mRNA species. J Neurosci Res 31: 606-615. https://doi.org/10.1002/jnr.490310404
  22. Manzerra P, Brown IR. 1996. The neuronal stress response: nuclear translocation of heat shock proteins as an indicator of hyperthermic stress. Exp Cell Res 229: 35-47. https://doi.org/10.1006/excr.1996.0341
  23. Mateus AP, Power DM, Canario AVM. 2017. Chapter 8-Stress and Disease in Fish. In Fish Diseases, Jeney G, Ed., Academic Press: London, UK, pp 187-220.
  24. Mayer MP, Bukau B. 2005. HSP70 chaperones: cellular functions and molecular mechanism. Cell Mol Life Sci 62: 670-684. https://doi.org/10.1007/s00018-004-4464-6
  25. Nam BH, An, CM, Kim DG, Kong HJ, Kang JH, Moon JY, Park JY, Kim WJ, Kim YO, Yoon J, Park CJ. 2017. Genome sequence of pacific abalone (Haliotis discus hannai): The first draft genome in family Haliotidae. GigaScience 6: gix014.
  26. Park CJ, Kim SY. 2013. Abalone Aquaculture in Korea. J Shellfish Res 32: 17-19. https://doi.org/10.2983/035.032.0104
  27. Pratt WB. 1997. The role of the HSP90-based chaperone system in signal transduction by nuclear receptors and receptors signaling via MAP kinase. Annu Rev Pharmacol Toxicol 37: 297-326. https://doi.org/10.1146/annurev.pharmtox.37.1.297
  28. Rochelle JM, Grossfeld RM, Bunting DL, Tytell M, Dwyer BE, Xue ZY. 1991. Stress protein synthesis by crayfish CNS tissue in vitro. Neurochem Res 16: 533-542. https://doi.org/10.1007/BF00974871
  29. Sarge KD, Murphy SP, Morimoto RI. 1993. Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress. Mol Cell Bio l13: 1392-1407.
  30. Sidera K, Samiotaki M, Yfanti E, Panayotou G, Patsavoudi E. 2004. Involvement of cell surface HSP90 in cell migration reveals a novel role in the developing nervous system. J Biol Chem 279: 45379-45388. https://doi.org/10.1074/jbc.M405486200
  31. Spees JL, Chang SA, Snyder MJ, Chang ES. 2002. Thermal acclimation and stress in the American lobster, Homarus americanus: equivalent temperature shifts elicit unique gene expression patterns for molecular chaperones and polyubiquitin. Cell Stress Chaperones 7: 97-106. https://doi.org/10.1379/1466-1268(2002)007<0097:TAASIT>2.0.CO;2
  32. Sukhan ZP, Sharker MR, Kho KH. 2020. Molecular cloning, in silico characterization and expression analysis of axonemal protein 66.0 in Pacific abalone, Haliotis discus hannai. Eur Zool J 87: 648-658. https://doi.org/10.1080/24750263.2020.1821800
  33. Turturici G, Sconzo G, Geraci F. 2011. HSP70 and its molecular role in nervous system diseases. Biochem Res Int 2011: 618127.
  34. Walsh D, Li Z, Wu Y, Nagata K. 1997. Heat shock and the role of the heat shock proteins during neural plate induction in early mammalian CNS and brain development. Cell Mol Life Sci 53: 198-211. https://doi.org/10.1007/PL00000592
  35. Wang N, Whang I, Lee JS, Lee J. 2011. Molecular characterization and expression analysis of a heat shock protein 90 gene from disk abalone (Haliotis discus). Mol Biol Rep 38: 3055-3060. https://doi.org/10.1007/s11033-010-9972-x
  36. Wegele H, Muller L, Buchner J. 2004. HSP70 and HSP90- a relay team for protein folding. Rev Physiol Biochem Pharmacol 151: 1-44. https://doi.org/10.1007/s10254-003-0021-1
  37. Zhang W, Wu C, Mai K, Chen Q, Xu W. 2011. Molecular cloning, characterization and expression analysis of heat shock protein 90 from Pacific abalone, Haliotis discus hannai Ino in response to dietary selenium. Fish Shelfish Immun 30: 280-286. https://doi.org/10.1016/j.fsi.2010.10.019