• Korean Journal of Microbiology
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• v.47 no.4
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• pp.289-294
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• 2011

We investigated the cold shock sensitivity of DEAD-box RNA helicase gene deleted strains of in Bacillus subtilis CU1065. To understand cold shock effects, cells were cultivated at $37^{\circ}C$ to log phase ($O.D_{600}$=0.5-0.6) and then temperature was shifted to $15^{\circ}C$. Cold shock slow down the growth rate of wild type and deleted strains of DEAD-box RNA helicase gene (ydbR, yfmL, yqfR, deaD). The growth rate of ydbR deleted strain is 5 times severely reduced compared to that of wild type strain (CU1065). But the growth rate of other three (yfmL, yqfR, deaD) deleted strains is nearly equal to the growth rate of wild type. Compared to $37^{\circ}C$, the amount of ydbR and yqfR mRNA transcripts are increased at the growth temperature of $15^{\circ}C$. On the other hands the mRNA transcripts of yfmL and deaD are not changed at both conditions of $37^{\circ}C$ and $15^{\circ}C$. Upon cold shock treatment ydbR mRNA transcript is clearly increased. After treatment of rifampicin (bacteria transcription inhibitor) the amount of ydbR mRNA was measured. Temperature shift from $37^{\circ}C$ to $15^{\circ}C$ and rifampicin treatment showed slowly decay of ydbR mRNA. But at $37^{\circ}C$ and rifampicin treatment ydbR mRNA is rapidly reduced. These results showed that cold shock induction of ydbR mRNA resulted from the stability of ydbR mRNA and not from the transcription induction of ydbR. In relation to these results, we found the cold box element of csp (cold shock protein gene) in 5' untranslated region of ydbR gene. Cold shock induction of ydbR is caused by the stability of ydbR mRNA like the stability of csp mRNA.

• Journal of Institute of Control, Robotics and Systems
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• v.10 no.2
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• pp.153-163
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• 2004

The control objective in hard disk drives is to move head as fast as possible to target track and position the head over the center of target track in the presence of external disturbances. The external shock or disk clamping error in manufacturing process causes the disk center to deviate from the disk rotation center. The disk shift acts on the control system as disturbance and degrades severely the performance of disk drives. In this paper, we present a new controller that compensates for the periodic disturbances very fast. The disturbance compensator is arranged in parallel with the state feedback controller. To avoid the interference with the state feedback controller, the compensator creates compensation signal without the feedback of system output until steady state. The pulse type controller is included additionally for improving the transient performance due to initial state. Finally, in order to demonstrate the superior performance of the proposed compensator. we present some experimental results using a commercially available disk drive.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.20 no.4
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• pp.376-381
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• 2011

CVT(Continuously Variable Transmission) is one of the most promising candidates for the future automobile transmission because of its continuously variable gear ratio and reduced shift shock. It is also possible to operate the power source at its high efficiency region with CVT. The CVT system consists of thrust plate, driving pulley, belt, driven pulley, and preload spring of output shaft. In this paper, the dynamic modeling of a CVT system is completed to obtain the static performances of CVT system. A simulator is implemented on Matlab(Simulink), which can analyse the static performances of a CVT system. The methods for improving the total efficiency of a motorcycle system are also proposed based on the simulation results. In this study we increase the capacity factor of CVT up to the three times of default specification.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2008.04a
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• pp.144-149
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• 2008

In this paper, vibration sources of a car-holder are identified and the car vibrations are reduced by structural modification. For vibration identification, vibration signals are measured by an accelerometer when the car is moving. From some experiments and finite element analyses, it is also found that resonances occur because the natural frequencies of the car exist in usual driving speed range. To shift the natural frequencies outside the driving speed range, the connection part is modified to increase stiffness. It is verified that considerable amount of vibration are reduced by the structural modification.

• BMB Reports
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• v.33 no.1
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• pp.75-81
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• 2000

DnaK is a major heat shock protein and known to be highly conserved in all species. Previously, the dnaK in Streptococcus pneumoniae was cloned and the immunogenic nature characterized. In this study, dnaK mutants were generated by insertion of duplication mutagenesis and their characteristics examined. They had defective growths at all temperatures ($20^{\circ}C-42^{\circ}C$)and cell divisions, and formed filaments after a temperature shift from 30 to 42. A unique feature of the dnaK mutants of S. pneumoniae, unlike those of E. coli and B. subtilis, was the growth capability at high temperature ($42^{\circ}C$) without producing the putative GroEL. Our results suggest that DnaK may serve as a regulator and/or modifier in GroEL gene expression.

• Journal of the Korea Institute of Building Construction
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• v.14 no.4
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• pp.350-358
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• 2014

The demand for the lightweight wall has increased as the structure of the multi-unit dwelling has shift to the rahmen structure. The lightweight wall is required to secure certain degree of shock resistance for the structural safety. The study performed the load analysis test for 7 small hard bodies with different masses and shapes and 5 hard materials which applied the impact load on the wall. It was found out from the experiment that different pendulum weight doubled the load maximum even though the shock energy was the same. In addition, the study compared and analyzed the weight of materials and the load of small hard bodies to propose fundamental data for the material design of the lightweight wall.

• Molecules and Cells
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• v.39 no.6
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• pp.477-483
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• 2016

Heat shock factors (Hsfs) are central regulators of abiotic stress responses, especially heat stress responses, in plants. In the current study, we characterized the activity of the Hsf gene HsfA3 in Arabidopsis under oxidative stress conditions. HsfA3 transcription in seedlings was induced by reactive oxygen species (ROS), exogenous hydrogen peroxide ($H_2O_2$), and an endogenous $H_2O_2$ propagator, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB). HsfA3-overexpressing transgenic plants exhibited increased oxidative stress tolerance compared to untransformed wild-type plants (WT), as revealed by changes in fresh weight, chlorophyll fluorescence, and ion leakage under light conditions. The expression of several genes encoding galactinol synthase (GolS), a key enzyme in the biosynthesis of raffinose family oligosaccharides (RFOs), which function as antioxidants in plant cells, was induced in HsfA3 overexpressors. In addition, galactinol levels were higher in HsfA3 overexpressors than in WT under unstressed conditions. In transient transactivation assays using Arabidopsis leaf protoplasts, HsfA3 activated the transcription of a reporter gene driven by the GolS1 or GolS2 promoter. Electrophoretic mobility shift assays showed that GolS1 and GolS2 are directly regulated by HsfA3. Taken together, these findings provide evidence that GolS1 and GolS2 are directly regulated by HsfA3 and that GolS enzymes play an important role in improving oxidative stress tolerance by increasing galactinol biosynthesis in Arabidopsis.

• Applied Chemistry for Engineering
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• v.22 no.4
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• pp.400-404
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• 2011

The activity and stability of the metal filter material Pt coated on NOx and soot oxidation were examined. The catalytic reaction test for NOx and soot were also performed independently and simultaneously. As a result, it showed the NO to $NO_2$ shift reaction with 20% conversion, NOx decomposition (about 10%) and perfect soot oxidation on the material Pt coated proceeded. Onset temperature of soot oxidation shift to lower temperature (about $30^{\circ}C$) by generated $NO_2$. The material also was less affected by thermal shock than $Pt/Al_2O_3$ or $Pt/TiO_3$ catalysts due to its stability of surface structure.

• Journal of Technology Innovation
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• v.31 no.3
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• pp.111-138
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• 2023

The purpose of this paper is to summarize the development process of major science and technology manpower policies and to derive desirable future policy directions in the policy environment of a paradigm shift in supply and demand. To this end, first, the development process of the science and technology manpower policy, which was promoted in earnest from the 2000s, with the topic of resolving the phenomenon of avoidance of science and engineering majors, was examined, focusing on the basic plan for supporting science and engineering majors. Next, we summarized the main contents and implications of the change in demand for science and technology personnel caused by digital transformation and the supply shock of demographic decline, that is, the paradigm shift in the supply and demand of science and technology talents. Based on this, the core direction of the future science and technology manpower policy was suggested to create a human-centered science and technology research ecosystem that promotes the continued growth and inflow of human resources, so that can be the foundation for the training and utilization of excellent science and technology talents.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.54-58
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• 2013

SAV0506 is an 87 residue hypothetical protein from Staphylococcus aureus strain Mu50 and also predicted to have similar function to ribosome associated heat shock protein, Hsp 15. Hsp15 is thought to be involved in the repair mechanism of erroneously produced 50S ribosome subunit. In this report, we present the sequence specific backbone resonance assignment of SAV0506. About 82.5% of all resonances could be assigned unambiguously. By analyzing deviations of the $C{\alpha}$ and $C{\beta}$ chemical shift values, we could predict the secondary structure of SAV0506. This study is an essential step towards the structural characterization of SAV0506.