• Title/Summary/Keyword: Sequence characteristics

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Characteristics of Microorganisms Contaminating Seafood Cooking Drips Exposed to Gamma Irradiation (감마선 조사된 수산 자숙액의 오염 미생물군 특성)

  • Choi, Jong-Il;Kim, Yeon-Joo;Kim, Jae-Hun;Chun, Byung-Soo;Ahn, Dong-Hyun;Kwon, Joong-Ho;Hwang, Young-Jung;Byun, Myung-Woo;Lee, Ju-Woon
    • Food Science and Preservation
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    • v.16 no.2
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    • pp.286-291
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    • 2009
  • Microorganisms in seafood cooking drips were counted and identified. Total viable cell counts were 6.40 and 3.10 log CFU/g in cooking drips of Hizikia fusiformis and Thunnus thynnus, respectively. However, microbial populations fell with increased irradiation doses. In H. fusiformis cooking drips, a 5-log reduction in total aerobic bacteria was obtained by irradiation with 5 kGy. In T. thynnus cooking drips, however, contaminating microorganisms were more resistant to gamma irradiation and only a 1-log reduction was seen. DNA sequence analysis showed that the principal contaminating microorganisms in H. fusiformis and T. thynnus cooking drips were Lactobacillus and Bacillus species, respectively. Therefore, the high irradiation resistance of T. thynnus cooking drips microbes may result from spore formation by Bacillus species.

Comparison and Performance analysis of Wavelet OFDM system and FD-OFDM (웨이블릿 OFDM 시스템과 FD-OFDM 시스템 성능 비교 분석)

  • Lee, Junseo;Kim, Ji-Hoon;Kim, Whanwoo
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.7
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    • pp.34-42
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    • 2013
  • In this paper, we compare the performance of wavelet OFDM (Orthogonal Frequency Division Multiplexing) and FD-OFDM(Frequency diversity OFDM) system with conventional OFDM system. Wavelet OFDM system uses wavelet transform rather than Fourier transform and contains intermediate characteristics of CDM (Code Division Multiplexing) and OFDM. In wavelet OFDM system, inter-symbol interference (ISI) can be suppressed effectively and adjacent channel interference can be also minimized well. In FD-OFDM system, each parallel branch symbol is multiplied by the orthogonal sequence and distributed into all sub-carriers. Then, each sub-carrier transmits information composed of the symbol components of all parallel branches in the given frame. FD-OFDM contains the frequency diversity characteristic and, therefore, FD-OFDM can be robust to the narrowband interference. For the comparison among different systems, BER (Bit-Error Rate) performances are evaluated in the presence of narrow-band interference and a harmonic noise channel. From the evaluation results, compared to the conventional OFDM, wavelet OFDM and FD-OFDM shows better robustness against the interference and, especially, wavelet OFDM is the most robust in harmonic noise channel.

Investigation of Fatigue Damage of the Mooring Lines for Submerged Floating Tunnels Under Irregular Waves (불규칙 파랑 중 해중 터널 계류선의 단기 피로 손상 분석)

  • Kim, Seungjun;Won, Deok Hee
    • Journal of Korean Society of Steel Construction
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    • v.29 no.1
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    • pp.49-60
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    • 2017
  • As well as the strength check, fatigue life check is also mainly required for designing mooring lines of the floating structures. In general, forces which induce dynamic structural response significantly affect to fatigue design of the mooring lines. So, waves are mainly considered as the governing loading for fatigue design of the mooring lines. In this study, characteristics of the fatigue damage of the mooring lines for submerged floating tunnels (SFT) under irregular waves are investigated. For this study time domain hydrodynamic analysis is used to obtain motion of the tunnel and tension and stresses of the mooring lines under the specific environmental conditions. Also, the Rainflow-counting method, the Palmgren-Miner's rule, and S-N curves for floating offshore structures presented by DNV recommendation is applied to calculate the fatigue damage due to the fluctuating stresses. Referring to the design plactice of the tendon pipes for TLP (tension-leg platform), which is very similar structural system to SFT, it is assumed that a 100 year return period wave attacks the SFT systems during 48 hours and the fatigue damages due to the environmental loading are calculated. Following the analysis sequence, the effects of the tunnel draft, spacing and initial inclination angle of the mooring lines on the fatigue damage under the specific environmental loadings are investigated.

Drawing Elements of Inquiry in Field Geology and Analyzing Field Geology Education in Previous Studies (야외 지질학 탐구 요소 추출 및 지질 답사 교육 문헌 분석)

  • Jung, Chanmi;Shin, Donghee
    • Journal of The Korean Association For Science Education
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    • v.37 no.3
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    • pp.465-481
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    • 2017
  • This study is a research synthesis analyzing how field geology education is conducted in domestic and foreign countries in the recent 20 years and how it reflects the characteristics of authentic geologic inquiry. For these purposes, we first drew five elements of inquiry in field geology (observation, representation, abductive reasoning, spatial thinking, and diachronic thinking) considering the field geologists' actual research method as well as its pedagogical significance in science education. We developed analysis criteria for field geology education. The 53 cases were analyzed based on each element of inquiry in field geology and its sub-elements, and also the tendency of overall elements. As a result, observation and representation were included in most cases, but there appeared less frequency in order of abductive reasoning, spatial thinking, and diachronic thinking. For observation, the ratio of purposive observation and autonomous observation is high. For representation, both visualizing and linguistic type of representation and free-form representation appear frequently. For abductive reasoning, the step of generating hypothesis is often included and the hypothesis tends to be about the geological formatting process. For spatial thinking, type of self-location and perception of the spatial configuration of the structure appear at a high rate. For diachronic thinking, type of stratigraphic sequence is the most frequent. The proportions of the cases including three or more elements of inquiry in field geology consist 87% of the total. We suggested implications for improving geological fieldwork as authentic science inquiry in the future.

A Study on the HEVC Video Encoder PMR Block Design (HEVC 비디오 인코더 PMR 블록 설계에 대한 연구)

  • Lee, Sukho;Lee, Jehyun
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.12
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    • pp.141-146
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    • 2016
  • HEVC/H.265 is the latest joint video coding standard proposed by ITU-T SG 16 WP and ISO/IEC JTC 1/SC29/WG 11. In H.265, pictures are divided into a sequence of coding tree units(CTUs), and the CTU further is partitioned into multiple CUs to adapt to various local characteristics. Its coding efficiency is approximately two times high compared to previous standard H.264/AVC. However according to the size of extended CU(coding unit) and transform block, the hardware size of PMR(prediction/mode decision/reconstruction) block within video encoder is about 4 times larger than previous standard. In this study, we propose a new less complex hardware architecture of PMR block which has the most high complexity within encoder without any noticeable PSNR loss. Using this simplified block, we can shrink the overall size the H.265 encoder. For FHD image, it operates at clocking frequency of 300 MHz and frame rate of 60 fps. And also for the test image, the Bjøntegaard Delta (BD) bit rate increase about average 30 % in PMR prediction block, and the total estimated gate count of PMR block is around 1.8 M.

A Framework of Recognition and Tracking for Underwater Objects based on Sonar Images : Part 2. Design and Implementation of Realtime Framework using Probabilistic Candidate Selection (소나 영상 기반의 수중 물체 인식과 추종을 위한 구조 : Part 2. 확률적 후보 선택을 통한 실시간 프레임워크의 설계 및 구현)

  • Lee, Yeongjun;Kim, Tae Gyun;Lee, Jihong;Choi, Hyun-Taek
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.3
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    • pp.164-173
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    • 2014
  • In underwater robotics, vision would be a key element for recognition in underwater environments. However, due to turbidity an underwater optical camera is rarely available. An underwater imaging sonar, as an alternative, delivers low quality sonar images which are not stable and accurate enough to find out natural objects by image processing. For this, artificial landmarks based on the characteristics of ultrasonic waves and their recognition method by a shape matrix transformation were proposed and were proven in Part 1. But, this is not working properly in undulating and dynamically noisy sea-bottom. To solve this, we propose a framework providing a selection phase of likelihood candidates, a selection phase for final candidates, recognition phase and tracking phase in sequence images, where a particle filter based selection mechanism to eliminate fake candidates and a mean shift based tracking algorithm are also proposed. All 4 steps are running in parallel and real-time processing. The proposed framework is flexible to add and to modify internal algorithms. A pool test and sea trial are carried out to prove the performance, and detail analysis of experimental results are done. Information is obtained from tracking phase such as relative distance, bearing will be expected to be used for control and navigation of underwater robots.

Full-Length Enriched cDNA Library Construction from Tissues Related to Energy Metabolism in Pigs

  • Lee, Kyung-Tai;Byun, Mi-Jeong;Lim, Dajeong;Kang, Kyung-Soo;Kim, Nam-Soon;Oh, Jung-Hwa;Chung, Chung-Soo;Park, Hae-Suk;Shin, Younhee;Kim, Tae-Hun
    • Molecules and Cells
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    • v.28 no.6
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    • pp.529-536
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    • 2009
  • Genome sequencing of the pig is being accelerated because of its importance as an evolutionary and biomedical model animal as well as a major livestock animal. However, information on expressed porcine genes is insufficient to allow annotation and use of the genomic information. A series of expressed sequence tags of 5' ends of five full-length enriched cDNA libraries (SUSFLECKs) were functionally characterized. SUSFLECKs were constructed from porcine abdominal fat, induced fat cells, loin muscle, liver, and pituitary gland, and were composed of non-normalized and normalized libraries. A total of 55,658 ESTs that were sequenced once from the 5′ ends of clones were produced and assembled into 17,684 unique sequences with 7,736 contigs and 9,948 singletons. In Gene Ontology analysis, two significant biological process leaf nodes were found: gluconeogenesis and translation elongation. In functional domain analysis based on the Pfam database, the beta transducin repeat domain of WD40 protein was the most frequently occurring domain. Twelve genes, including SLC25A6, EEF1G, EEF1A1, COX1, ACTA1, SLA, and ANXA2, were significantly more abundant in fat tissues than in loin muscle, liver, and pituitary gland in the SUSFLECKs. These characteristics of SUSFLECKs determined by EST analysis can provide important insight to discover the functional pathways in gene networks and to expand our understanding of energy metabolism in the pig.

Dose Verification of Intensity Modulated Radiation Therapy with Beam Intensity Scanner System

  • Vahc, Young-Woo;Park, Kwangyl;Ohyun Kwon;Park, Kyung-Ran;Lee, Yong-Ha;Yi, Byung-Yong;Kim, Sookil
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.248-251
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    • 2002
  • The intensity modulated radiation therapy (IMRT) with a multileaf collimator (MLC) requires the conversion of a radiation fluence map into a leaf sequence file that controls the movement of the MLC during radiation treatment of patients. Patient dose verification is clinically one of the most important parts in the treatment delivery of the radiation therapy. The three dimensional (3D) reconstruction of dose distribution delivered to the target helps to verify patient dose and to determine the physical characteristics of beams used in IMRT. A new method is presented for the pretreatment dosimetric verification of two dimensional distributions of photon intensity by means of Beam Intensity Scanner System (BISS) as a radiation detector with a custom-made software for dose calculation of fluorescence signals from scintillator. The scintillator is used to produce fluorescence from the irradiation of 6MV photons on a Varian Clinac 21EX. The BISS reproduces 3D- relative dose distribution from the digitized fluoroscopic signals obtained by digital video camera-based scintillator(DVCS) device in the IMRT. For the intensity modulated beams (IMBs), the calculations of absorbed dose are performed in absolute beam fluence profiles which are used for calculation of the patient dose distribution. The 3D-dose profiles of the IMBs with the BISS were demonstrated by relative measurements of photon beams and shown good agreement with radiographic film. The mechanical and dosimetric properties of the collimating of dynamic and/or step MLC system alter the generated intensity. This is mostly due to leaf transmission, leaf penumbra and geometry of leaves. The variations of output according to the multileaf opening during the irradiation need to be accounted for as well. These phenomena result in a fluence distribution that can be substantially different from the initial and calculative intensity modulation and therefore, should be taken into account by the treatment planning for accurate dose calculations delivered to the target volume in IMRT.

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Alternaria Leaf Spot Caused by Alternaria mali on Black Chokeberry in Korea (Alternaria mali에 의한 아로니아 점무늬낙엽병)

  • Hahm, Soo-Sang;Kwon, Mi-Kyung;Kim, Byung-Ryun;Han, Kwang-Seop;Nam, Yun-Gyu
    • Research in Plant Disease
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    • v.22 no.1
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    • pp.50-54
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    • 2016
  • In early June 2014, leaf spot symptoms were observed on black chokeberry (Aronia melanocarpa) in Yesan-gun and Geumsan-gun, Chungcheongnam-do in Korea. The initial symptoms on leave surfaces were brown small-circular spots with a yellow halo lesion, and gradually the small spots were fused, all of infected leaves dropped eventually. A fungus were isolated from the initial lesion, and cultured on potato dextrose agar. Colony color on upper surface of plate varied from olive gray to charcoal gray. Size of conidia mostly extend to $19-50{\times}5-9{\mu}m$ in nature and $20-59{\times}8-13{\mu}m$ in culture, with 3-8 transverse septa and usually no longitudinal septum or only 1 longitudinal septum in 1-3 of the transverse compartments, and also have a short or long beak. Pathogenicity was investigated using wounded or unwounded black chokeberry and apple leaves. After 7 days of inoculation, leaf spots were similar to the symptoms naturally occurred in the field. On the basis of mycological characteristics, pathogenicity, and ITS rDNA sequence analysis, this fungus was identified as Alternaria mali. This is the first report of Alternaria leaf spot on black chokeberry caused by A. mali in Korea.

Stalk Rot Caused by Fusarium thapsinum on Sorghum at Organic Paddy-upland Rotation System in Korea (유기농 답전윤환지 Fusarium thapsinum에 의한 수수 줄기썩음병)

  • Kim, Byung-Ryun;Han, Kwang-Seop;Hahm, Soo-Sang;Kang, Young-Sik;Park, In-Hee;Yoon, Seong Tak
    • Research in Plant Disease
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    • v.22 no.4
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    • pp.293-296
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    • 2016
  • In late June 2016, stalk rot symptoms were observed on five vatieties of sorghum (Sorghum bicolar) at organic paddy-upland rotation system in Anseong city, Korea. The initial symptom on stalk surfaces was red color with a dark red spot lesion. A fungus was isolated from the initial lesion, and cultured on potato dextrose agar. Size of microconidia mostly extend to $5-19{\times}2-{\mu}m$ in culture, with 0-1 septa and macroconidia extend to $29-52{\times}3-4{\mu}m$ with 4-6 septa. Pathogenicity was investigated using conidial suspension spray to seedling of sorghum. After 3 days of inoculation, the dark red lesion was produced on stalks. On the basis of mycological characteristics, pathogenicity, and internal transcribed spacer (ITS) rDNA sequence analysis, this fungus was identified as Fusarium thapsinum. This is the first report of stalk rot on sorghum caused by F. thapsinum in Korea.